8xow - Revision history

OCA at 19:59, 29 May 2024

OCA — Wed, 29 May 2024 19:59:02 GMT

←Older revision		Revision as of 19:59, 29 May 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/FII_LAMBD FII_LAMBD] Plays a role in virion assembly by joining the head and the tail at the last step of morphogenesis. Six FII proteins bind the DNA-filled capsid after W binding, in turn binding the pre-assembled tail by interacting with U protein.<ref>PMID:4413519</ref> <ref>PMID:4616093</ref> <ref>PMID:5022189</ref>		[https://www.uniprot.org/uniprot/FII_LAMBD FII_LAMBD] Plays a role in virion assembly by joining the head and the tail at the last step of morphogenesis. Six FII proteins bind the DNA-filled capsid after W binding, in turn binding the pre-assembled tail by interacting with U protein.<ref>PMID:4413519</ref> <ref>PMID:4616093</ref> <ref>PMID:5022189</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The portal protein of tailed bacteriophage plays essential roles in various aspects of capsid assembly, motor assembly, genome packaging, connector formation, and infection processes. After DNA packaging is complete, additional proteins are assembled onto the portal to form the connector complex, which is crucial as it bridges the mature head and tail. In this study, we report high-resolution cryo-electron microscopy (cryo-EM) structures of the portal vertex from bacteriophage lambda in both its prohead and mature virion states. Comparison of these structures shows that during head maturation, in addition to capsid expansion, the portal protein undergoes conformational changes to establish interactions with the connector proteins. Additionally, the independently assembled tail undergoes morphological alterations at its proximal end, facilitating its connection to the head-tail joining protein and resulting in the formation of a stable portal-connector-tail complex. The B-DNA molecule spirally glides through the tube, interacting with the nozzle blade region of the middle-ring connector protein. These insights elucidate a mechanism for portal maturation and DNA translocation within the phage lambda system. IMPORTANCE: The tailed bacteriophages possess a distinct portal vertex that consists of a ring of 12 portal proteins associated with a 5-fold capsid shell. This portal protein is crucial in multiple stages of virus assembly and infection. Our research focused on examining the structures of the portal vertex in both its preliminary prohead state and the fully mature virion state of bacteriophage lambda. By analyzing these structures, we were able to understand how the portal protein undergoes conformational changes during maturation, the mechanism by which it prevents DNA from escaping, and the process of DNA spirally gliding.
		+
		+	Structural morphing in the viral portal vertex of bacteriophage lambda.,Gu Z, Wu K, Wang J J Virol. 2024 May 14;98(5):e0006824. doi: 10.1128/jvi.00068-24. Epub 2024 Apr 25. PMID:38661364<ref>PMID:38661364</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 8xow" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:04, 10 April 2024

OCA — Wed, 10 Apr 2024 08:04:31 GMT

←Older revision		Revision as of 08:04, 10 April 2024
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-	'''Unreleased structure'''

-	~~The entry~~ 8xow is ~~ON HOLD until Paper Publication~~	+	==Mature virion portal of bacteriophage lambda==
-		+	<StructureSection load='8xow' size='340' side='right'caption='[[8xow]], [[Resolution\|resolution]] 3.32Å' scene=''>
-	~~Authors~~: ~~Wang~~, J.W., Gu, Z.W.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[8xow]] is a 36 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_Lambda Escherichia phage Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8XOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8XOW FirstGlance]. <br>
-	~~Description~~: ~~Mature~~ virion ~~portal~~ of ~~bacteriophage lambda~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.32Å</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8xow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8xow OCA], [https://pdbe.org/8xow PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8xow RCSB], [https://www.ebi.ac.uk/pdbsum/8xow PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8xow ProSAT]</span></td></tr>
-	[[Category: ~~Wang, J.W~~]]	+	</table>
-	[[Category: Gu~~, Z.W~~]]	+	== Function ==
		+	[https://www.uniprot.org/uniprot/FII_LAMBD FII_LAMBD] Plays a role in virion assembly by joining the head and the tail at the last step of morphogenesis. Six FII proteins bind the DNA-filled capsid after W binding, in turn binding the pre-assembled tail by interacting with U protein.<ref>PMID:4413519</ref> <ref>PMID:4616093</ref> <ref>PMID:5022189</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia phage Lambda]]
		+	[[Category: Large Structures]]
		+	[[Category: Gu ZW]]
		+	[[Category: Wang JW]]

OCA at 10:30, 24 January 2024

OCA — Wed, 24 Jan 2024 10:30:41 GMT

←Older revision		Revision as of 10:30, 24 January 2024
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8xow is ON HOLD	+	The entry 8xow is ON HOLD until Paper Publication

	Authors: Wang, J.W., Gu, Z.W.		Authors: Wang, J.W., Gu, Z.W.

OCA: Protected "8xow" [edit=sysop:move=sysop]

OCA — Wed, 10 Jan 2024 09:31:16 GMT

Protected "8xow" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:31, 10 January 2024

OCA: New page: '''Unreleased structure''' The entry 8xow is ON HOLD Authors: Wang, J.W., Gu, Z.W. Description: Mature virion portal of bacteriophage lambda Category: Unreleased Structures [[Categ...

OCA — Wed, 10 Jan 2024 09:31:14 GMT

New page: '''Unreleased structure''' The entry 8xow is ON HOLD Authors: Wang, J.W., Gu, Z.W. Description: Mature virion portal of bacteriophage lambda Category: Unreleased Structures [[Categ...

New page

'''Unreleased structure'''

The entry 8xow is ON HOLD

Authors: Wang, J.W., Gu, Z.W.

Description: Mature virion portal of bacteriophage lambda
[[Category: Unreleased Structures]]
[[Category: Wang, J.W]]
[[Category: Gu, Z.W]]