9est - Revision history

OCA at 03:50, 9 February 2016

OCA — Tue, 09 Feb 2016 03:50:04 GMT

←Older revision		Revision as of 03:50, 9 February 2016
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	<StructureSection load='9est' size='340' side='right' caption='[[9est]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='9est' size='340' side='right' caption='[[9est]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[9est]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sus_scrofa Sus scrofa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9EST OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9EST FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[9est]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9EST OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9EST FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IBR:(2-BROMOETHYL)(2-FORMYL-4-AMINOPHENYL)+ACETATE'>IBR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IBR:(2-BROMOETHYL)(2-FORMYL-4-AMINOPHENYL)+ACETATE'>IBR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9est FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9est OCA], [http://www.rcsb.org/pdb/explore.do?structureId=9est RCSB], [http://www.ebi.ac.uk/pdbsum/9est PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9est FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9est OCA], [http://pdbe.org/9est PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=9est RCSB], [http://www.ebi.ac.uk/pdbsum/9est PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~ELA1_PIG ELA1_PIG~~]] Acts upon elastin.	+	[[http://www.uniprot.org/uniprot/CELA1_PIG CELA1_PIG]] Acts upon elastin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=9est ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 9est" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Pancreatic elastase]]		[[Category: Pancreatic elastase]]
-	[[Category: ~~Sus scrofa~~]]	+	[[Category: Pig]]
	[[Category: Meyer, E F]]		[[Category: Meyer, E F]]
	[[Category: Powers, J C]]		[[Category: Powers, J C]]
	[[Category: Radhakrishnan, R]]		[[Category: Radhakrishnan, R]]

OCA at 04:13, 25 December 2014

OCA — Thu, 25 Dec 2014 04:13:09 GMT

←Older revision		Revision as of 04:13, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9est FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9est OCA], [http://www.rcsb.org/pdb/explore.do?structureId=9est RCSB], [http://www.ebi.ac.uk/pdbsum/9est PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9est FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9est OCA], [http://www.rcsb.org/pdb/explore.do?structureId=9est RCSB], [http://www.ebi.ac.uk/pdbsum/9est PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ELA1_PIG ELA1_PIG]] Acts upon elastin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:20, 18 December 2014

OCA — Thu, 18 Dec 2014 08:20:15 GMT

←Older revision		Revision as of 08:20, 18 December 2014
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-	[[~~Image~~:9est.~~png\|left~~\|200px]]	+	==STRUCTURAL STUDY OF PORCINE PANCREATIC ELASTASE COMPLEXED WITH 7-AMINO-3-(2-BROMOETHOXY)-4-CHLOROISOCOUMARIN AS A NONREACTIVATABLE DOUBLY COVALENT ENZYME-INHIBITOR COMPLEX==
		+	<StructureSection load='9est' size='340' side='right' caption='[[9est]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[9est]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9EST OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9EST FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IBR:(2-BROMOETHYL)(2-FORMYL-4-AMINOPHENYL)+ACETATE'>IBR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9est FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9est OCA], [http://www.rcsb.org/pdb/explore.do?structureId=9est RCSB], [http://www.ebi.ac.uk/pdbsum/9est PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/es/9est_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The complex of porcine pancreatic elastase (PPE) with 7-amino-3-(2-bromoethoxy)-4-chloroisocoumarin, a potent mechanism-based inhibitor, was crystallized and the crystal structure determined at 1.9-A resolution with a final R factor of 17.1%. The unbiased difference Fourier electron density map showed continuous density from O gamma of Ser 195 to the benzoyl carbonyl carbon atom and from N epsilon 2 of His 57 to the carbon atom at the 4-position of the isocoumarin ring in the inhibitor. This suggested unambiguously that the inhibitor was doubly covalently bound to the enzyme. It represents the first structural evidence for irreversible binding of an isocoumarin inhibitor to PPE through both Ser 195 and His 57 in the active site. The PPE-inhibitor complex is only partially activated in solution by hydroxylamine and confirms the existence of the doubly covalently bound complex along with the acyl enzyme. The benzoyl carbonyl oxygen atom of the inhibitor is not situated in the oxyanion hole formed by the amide (greater than NH) groups of Gly 193 and Ser 195. The complex is stabilized by the hydrogen-bonding interactions in the active site (from the N epsilon 2 of Gln 192 to the bromine atom in the inhibitor and the amino group at the 7-position of the isocoumarin ring to the carbonyl oxygen of Thr 41) and by van der Waals interactions. The inhibition rates of several 7-substituted 4-chloro-3-(bromoalkoxy)isocoumarins toward PPE were measured.(ABSTRACT TRUNCATED AT 250 WORDS)

-	~~{{STRUCTURE_9est\| PDB=9est \| SCENE= }}~~	+	Structural study of porcine pancreatic elastase complexed with 7-amino-3-(2-bromoethoxy)-4-chloroisocoumarin as a nonreactivatable doubly covalent enzyme-inhibitor complex.,Vijayalakshmi J, Meyer EF Jr, Kam CM, Powers JC Biochemistry. 1991 Feb 26;30(8):2175-83. PMID:1998677<ref>PMID:1998677</ref>

-	~~===STRUCTURAL STUDY OF PORCINE PANCREATIC ELASTASE COMPLEXED WITH 7-AMINO-3-(2-BROMOETHOXY)-4-CHLOROISOCOUMARIN AS A NONREACTIVATABLE DOUBLY COVALENT ENZYME-INHIBITOR COMPLEX===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_1998677}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[9est]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Sus_scrofa Sus scrofa]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=9EST OCA].~~	+

	==See Also==		==See Also==
	*[[Elastase\|Elastase]]		*[[Elastase\|Elastase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:001998677<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Pancreatic elastase]]		[[Category: Pancreatic elastase]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
-	[[Category: Meyer, E F.]]	+	[[Category: Meyer, E F]]
-	[[Category: Powers, J C.]]	+	[[Category: Powers, J C]]
-	[[Category: Radhakrishnan, R.]]	+	[[Category: Radhakrishnan, R]]

OCA at 12:32, 20 October 2012

OCA — Sat, 20 Oct 2012 12:32:51 GMT

←Older revision		Revision as of 12:32, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[9est]] is a 1 chain structure ~~of [[Elastase]]~~ with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9EST OCA].	+	[[9est]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9EST OCA].

	==See Also==		==See Also==

OCA at 09:04, 26 July 2012

OCA — Thu, 26 Jul 2012 09:04:39 GMT

←Older revision		Revision as of 09:04, 26 July 2012
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	[[Image:9est.png\|left\|200px]]		[[Image:9est.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_9est", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_9est\| PDB=9est \| SCENE= }}		{{STRUCTURE_9est\| PDB=9est \| SCENE= }}

	===STRUCTURAL STUDY OF PORCINE PANCREATIC ELASTASE COMPLEXED WITH 7-AMINO-3-(2-BROMOETHOXY)-4-CHLOROISOCOUMARIN AS A NONREACTIVATABLE DOUBLY COVALENT ENZYME-INHIBITOR COMPLEX===		===STRUCTURAL STUDY OF PORCINE PANCREATIC ELASTASE COMPLEXED WITH 7-AMINO-3-(2-BROMOETHOXY)-4-CHLOROISOCOUMARIN AS A NONREACTIVATABLE DOUBLY COVALENT ENZYME-INHIBITOR COMPLEX===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_1998677}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 1998677 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_1998677}}		{{ABSTRACT_PUBMED_1998677}}

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	==See Also==		==See Also==
-	*[[Elastase]]	+	*[[Elastase\|Elastase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~1998677~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:001998677</ref><references group="xtra"/>
	[[Category: Pancreatic elastase]]		[[Category: Pancreatic elastase]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]

OCA: Protected "9est" [edit=sysop:move=sysop]

OCA — Sat, 25 Dec 2010 18:03:10 GMT

Protected "9est" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:03, 25 December 2010

OCA at 18:03, 25 December 2010

OCA — Sat, 25 Dec 2010 18:03:08 GMT

←Older revision		Revision as of 18:03, 25 December 2010
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-	{{Seed}}
	[[Image:9est.png\|left\|200px]]		[[Image:9est.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
-	~~9EST~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9EST OCA].	+	[[9est]] is a 1 chain structure of [[Elastase]] with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9EST OCA].
		+
		+	==See Also==
		+	*[[Elastase]]

	==Reference==		==Reference==
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	[[Category: Powers, J C.]]		[[Category: Powers, J C.]]
	[[Category: Radhakrishnan, R.]]		[[Category: Radhakrishnan, R.]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 7 08:04:53 2010''

OCA at 03:59, 7 July 2010

OCA — Wed, 07 Jul 2010 03:59:23 GMT

←Older revision		Revision as of 03:59, 7 July 2010
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	9EST is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9EST OCA].	+	9EST is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9EST OCA].

	==Reference==		==Reference==
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	[[Category: Pancreatic elastase]]		[[Category: Pancreatic elastase]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
-	[[Category: ~~Meyerjunior~~, E F.]]	+	[[Category: Meyer, E F.]]
	[[Category: Powers, J C.]]		[[Category: Powers, J C.]]
	[[Category: Radhakrishnan, R.]]		[[Category: Radhakrishnan, R.]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Feb 17 06~~:14:~~36 2009~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 7 08:04:53 2010''

OCA at 04:14, 17 February 2009

OCA — Tue, 17 Feb 2009 04:14:37 GMT

←Older revision		Revision as of 04:14, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	9EST is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9EST OCA].	+	9EST is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9EST OCA].

	==Reference==		==Reference==
-	Structural study of porcine pancreatic elastase complexed with 7-amino-3-(2-bromoethoxy)-4-chloroisocoumarin as a nonreactivatable doubly covalent enzyme-inhibitor complex., Vijayalakshmi J, Meyer EF Jr, Kam CM, Powers JC, Biochemistry. 1991 Feb 26;30(8):2175-83. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/1998677 1998677]~~	+	<ref group="xtra">PMID:1998677</ref><references group="xtra"/>
	[[Category: Pancreatic elastase]]		[[Category: Pancreatic elastase]]
-	[[Category: Single protein]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Meyerjunior, E F.]]		[[Category: Meyerjunior, E F.]]
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	[[Category: Radhakrishnan, R.]]		[[Category: Radhakrishnan, R.]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri Jul 11 12~~:53:~~08 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 06:14:36 2009''

OCA at 09:53, 11 July 2008

OCA — Fri, 11 Jul 2008 09:53:09 GMT

←Older revision		Revision as of 09:53, 11 July 2008
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-	[[Image:9est.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:9est.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_9est\| PDB=9est \| SCENE= }}		{{STRUCTURE_9est\| PDB=9est \| SCENE= }}

-	~~'''~~STRUCTURAL STUDY OF PORCINE PANCREATIC ELASTASE COMPLEXED WITH 7-AMINO-3-(2-BROMOETHOXY)-4-CHLOROISOCOUMARIN AS A NONREACTIVATABLE DOUBLY COVALENT ENZYME-INHIBITOR COMPLEX~~'''~~	+	===STRUCTURAL STUDY OF PORCINE PANCREATIC ELASTASE COMPLEXED WITH 7-AMINO-3-(2-BROMOETHOXY)-4-CHLOROISOCOUMARIN AS A NONREACTIVATABLE DOUBLY COVALENT ENZYME-INHIBITOR COMPLEX===


-	~~==Overview==~~	+	<!--
-	The ~~complex of porcine pancreatic elastase (PPE) with 7-amino-3-(2-bromoethoxy)-4-chloroisocoumarin~~, ~~a potent mechanism-based inhibitor~~, ~~was crystallized and~~ the ~~crystal structure determined at 1.9-A resolution with a final R factor of 17.1%. The unbiased difference Fourier electron density map showed continuous density from O gamma of Ser 195~~ to the ~~benzoyl carbonyl carbon atom and from N epsilon 2 of His 57 to the carbon atom~~ at ~~the 4-position of the isocoumarin ring in the inhibitor~~. ~~This suggested unambiguously that the inhibitor was doubly covalently bound to the enzyme~~. It represents the first structural evidence for irreversible binding of an isocoumarin inhibitor to PPE through both Ser 195 and His 57 in the active site. The PPE-inhibitor complex is only partially activated in solution by hydroxylamine and confirms the existence of the doubly covalently bound complex along with the acyl enzyme. The benzoyl carbonyl oxygen atom of the inhibitor is not situated in the oxyanion hole formed by the amide (greater than NH) ~~groups of Gly 193 and Ser 195. The complex~~ is ~~stabilized by~~ the hydrogen-bonding interactions in the active site (from the N epsilon 2 of Gln 192 to the bromine atom in the inhibitor and the amino group at the 7-position of the isocoumarin ring to the carbonyl oxygen of Thr 41) and by van der Waals interactions. ~~The inhibition rates of several 7~~-~~substituted 4~~-~~chloro-3-(bromoalkoxy)isocoumarins toward PPE were measured.(ABSTRACT TRUNCATED AT 250 WORDS)~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_1998677}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 1998677 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_1998677}}

	==About this Structure==		==About this Structure==
Line 26:		Line 30:
	[[Category: Powers, J C.]]		[[Category: Powers, J C.]]
	[[Category: Radhakrishnan, R.]]		[[Category: Radhakrishnan, R.]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 22~~:52:47 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Jul 11 12:53:08 2008''