OCA at 05:32, 17 April 2024

OCA — Wed, 17 Apr 2024 05:32:40 GMT

←Older revision		Revision as of 05:32, 17 April 2024
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 9ewo is ~~ON HOLD~~	+	==Mpro from SARS-CoV-2 with R4A R298A double mutations==
-		+	<StructureSection load='9ewo' size='340' side='right'caption='[[9ewo]], [[Resolution\|resolution]] 3.00Å' scene=''>
-	~~Authors~~: ~~Plewka~~, J., ~~Lis~~, K., ~~Chykunova~~, Y., ~~Czarna~~, A., ~~Kantyka~~, T., ~~Pyrc, K~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[9ewo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9EWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9EWO FirstGlance]. <br>
-	~~Description~~: ~~Mpro~~ from ~~SARS~~-~~CoV~~-2 ~~with R4A R298A double mutations~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.002Å</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[Category: ~~Plewka, J~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ewo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ewo OCA], [https://pdbe.org/9ewo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ewo RCSB], [https://www.ebi.ac.uk/pdbsum/9ewo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ewo ProSAT]</span></td></tr>
-	[[Category: Chykunova, Y]]	+	</table>
-	[[Category: ~~Pyrc, K~~]]	+	== Function ==
-	[[Category: Lis, K]]	+	[https://www.uniprot.org/uniprot/R1A_SARS2 R1A_SARS2] Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.[UniProtKB:P0C6X7] Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.[UniProtKB:P0C6X7] May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.[UniProtKB:P0C6X7] Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B signaling.[UniProtKB:P0C6X7] Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.[UniProtKB:P0C6X7] Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Also able to bind an ADP-ribose-1''-phosphate (ADRP).[UniProtKB:P0C6X7] Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.[UniProtKB:P0C6X7] Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[UniProtKB:P0C6X7] Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[UniProtKB:P0C6X7] May participate in viral replication by acting as a ssRNA-binding protein.[UniProtKB:P0C6X7] Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.[UniProtKB:P0C6X7]
-	[[Category: ~~Kantyka, T~~]]	+	__TOC__
-	[[Category: ~~Czarna, A~~]]	+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Chykunova Y]]
		+	[[Category: Czarna A]]
		+	[[Category: Kantyka T]]
		+	[[Category: Lis K]]
		+	[[Category: Plewka J]]
		+	[[Category: Pyrc K]]

OCA: Protected "9ewo" [edit=sysop:move=sysop]

OCA — Wed, 10 Apr 2024 06:56:54 GMT

Protected "9ewo" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:56, 10 April 2024

OCA: New page: '''Unreleased structure''' The entry 9ewo is ON HOLD Authors: Plewka, J., Lis, K., Chykunova, Y., Czarna, A., Kantyka, T., Pyrc, K. Description: Mpro from SARS-CoV-2 with R4A R298A dou...

OCA — Wed, 10 Apr 2024 06:56:53 GMT

New page: '''Unreleased structure''' The entry 9ewo is ON HOLD Authors: Plewka, J., Lis, K., Chykunova, Y., Czarna, A., Kantyka, T., Pyrc, K. Description: Mpro from SARS-CoV-2 with R4A R298A dou...

New page

'''Unreleased structure'''

The entry 9ewo is ON HOLD

Authors: Plewka, J., Lis, K., Chykunova, Y., Czarna, A., Kantyka, T., Pyrc, K.

Description: Mpro from SARS-CoV-2 with R4A R298A double mutations
[[Category: Unreleased Structures]]
[[Category: Plewka, J]]
[[Category: Chykunova, Y]]
[[Category: Pyrc, K]]
[[Category: Lis, K]]
[[Category: Kantyka, T]]
[[Category: Czarna, A]]

9ewo - Revision history

OCA at 05:32, 17 April 2024

OCA: Protected "9ewo" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 9ewo is ON HOLD Authors: Plewka, J., Lis, K., Chykunova, Y., Czarna, A., Kantyka, T., Pyrc, K. Description: Mpro from SARS-CoV-2 with R4A R298A dou...