9gaa - Revision history

OCA at 18:20, 20 September 2023

OCA — Wed, 20 Sep 2023 18:20:07 GMT

←Older revision		Revision as of 18:20, 20 September 2023
Line 1:		Line 1:

	==PRECURSOR OF THE T152A MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM==		==PRECURSOR OF THE T152A MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM==
-	<StructureSection load='9gaa' size='340' side='right' caption='[[9gaa]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='9gaa' size='340' side='right'caption='[[9gaa]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[9gaa]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_13253 Atcc 13253~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GAA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=9GAA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[9gaa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GAA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9GAA FirstGlance]. <br>
-	</td></tr><tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"~~><span class~~=~~'plainlinks'~~>~~[http://en.wikipedia.org/wiki/N(4)~~-~~(beta-N-acetylglucosaminyl)-L-asparaginase N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase]~~, ~~with EC number~~ [~~http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5~~.1~~.26 3.5.1.26] </span>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=9gaa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9gaa OCA], [~~http~~://pdbe.org/9gaa PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=9gaa RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/9gaa PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=9gaa ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9gaa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9gaa OCA], [https://pdbe.org/9gaa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9gaa RCSB], [https://www.ebi.ac.uk/pdbsum/9gaa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9gaa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ASPG_ELIMR ASPG_ELIMR]] Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins. Requires that the glycosylated asparagine moiety is not substituted on its N-(R1) and C- (R2) terminus.	+	[https://www.uniprot.org/uniprot/ASPG_ELIMR ASPG_ELIMR] Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins. Requires that the glycosylated asparagine moiety is not substituted on its N-(R1) and C- (R2) terminus.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:

	==See Also==		==See Also==
		+	*[[Asparaginase 3D structures\|Asparaginase 3D structures]]
	*[[Glycosylasparaginase\|Glycosylasparaginase]]		*[[Glycosylasparaginase\|Glycosylasparaginase]]
	== References ==		== References ==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 13253~~]]	+	[[Category: Elizabethkingia meningoseptica]]
-	[[Category: ~~Guo, H C~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Xu, Q]]~~	+	[[Category: Guo H-C]]
-	~~[[Category: Autoproteolysis]]~~	+	[[Category: Xu Q]]
-	~~[[Category: Glycosylasparaginase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Mutant]]~~	+
-	~~[[Category: N~~-~~terminal nucleophile hydrolase~~]]	+
-	[[Category: ~~Precursor~~]]	+

OCA at 07:06, 14 March 2018

OCA — Wed, 14 Mar 2018 07:06:13 GMT

←Older revision		Revision as of 07:06, 14 March 2018
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ga/9gaa_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ga/9gaa_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 19:53, 11 August 2016

OCA — Thu, 11 Aug 2016 19:53:47 GMT

←Older revision		Revision as of 19:53, 11 August 2016
Line 1:		Line 1:
		+
	==PRECURSOR OF THE T152A MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM==		==PRECURSOR OF THE T152A MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM==
	<StructureSection load='9gaa' size='340' side='right' caption='[[9gaa]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='9gaa' size='340' side='right' caption='[[9gaa]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[9gaa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Elizabethkingia_meningoseptica Elizabethkingia meningoseptica~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9GAA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[9gaa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_13253 Atcc 13253]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9GAA FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.26 3.5.1.26] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.26 3.5.1.26] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9gaa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9gaa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=9gaa RCSB], [http://www.ebi.ac.uk/pdbsum/9gaa PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9gaa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9gaa OCA], [http://pdbe.org/9gaa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=9gaa RCSB], [http://www.ebi.ac.uk/pdbsum/9gaa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=9gaa ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ASPG_ELIMR ASPG_ELIMR]] Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins. Requires that the glycosylated asparagine moiety is not substituted on its N-(R1) and C- (R2) terminus.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 14:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=9gaa ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 24:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 9gaa" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 31:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Elizabethkingia meningoseptica~~]]	+	[[Category: Atcc 13253]]
	[[Category: Guo, H C]]		[[Category: Guo, H C]]
	[[Category: Xu, Q]]		[[Category: Xu, Q]]

OCA at 08:45, 5 January 2015

OCA — Mon, 05 Jan 2015 08:45:45 GMT

←Older revision		Revision as of 08:45, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[9gaa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9GAA FirstGlance]. <br>		<table><tr><td colspan='2'>[[9gaa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9GAA FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.26 3.5.1.26] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.26 3.5.1.26] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9gaa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9gaa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=9gaa RCSB], [http://www.ebi.ac.uk/pdbsum/9gaa PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9gaa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9gaa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=9gaa RCSB], [http://www.ebi.ac.uk/pdbsum/9gaa PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[Glycosylasparaginase\|Glycosylasparaginase]]
	== References ==		== References ==
	<references/>		<references/>
Line 29:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Elizabethkingia meningoseptica]]		[[Category: Elizabethkingia meningoseptica]]
-	[[Category: Guo, H C.]]	+	[[Category: Guo, H C]]
-	[[Category: Xu, Q.]]	+	[[Category: Xu, Q]]
	[[Category: Autoproteolysis]]		[[Category: Autoproteolysis]]
	[[Category: Glycosylasparaginase]]		[[Category: Glycosylasparaginase]]

OCA: Protected "9gaa" [edit=sysop:move=sysop]

OCA — Thu, 05 Jun 2014 09:17:51 GMT

Protected "9gaa" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:17, 5 June 2014

OCA at 09:17, 5 June 2014

OCA — Thu, 05 Jun 2014 09:17:49 GMT

←Older revision		Revision as of 09:17, 5 June 2014
Line 1:		Line 1:
-	~~{{Seed}}~~	+	==PRECURSOR OF THE T152A MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM==
-	[[~~Image~~:9gaa.~~png\|left~~\|200px]]	+	<StructureSection load='9gaa' size='340' side='right' caption='[[9gaa]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[9gaa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9GAA FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.26 3.5.1.26] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9gaa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9gaa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=9gaa RCSB], [http://www.ebi.ac.uk/pdbsum/9gaa PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ga/9gaa_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A variety of proteins, including glycosylasparaginase, have recently been found to activate functions by self-catalyzed peptide bond rearrangements from single-chain precursors. Here we present the 1.9 A crystal structures of glycosylasparaginase precursors that are able to autoproteolyze via an N --> O acyl shift. Several conserved residues are aligned around the scissile peptide bond that is in a highly strained trans peptide bond configuration. The structure illustrates how a nucleophilic side chain may attack the scissile peptide bond at the immediate upstream backbone carbonyl and provides an understanding of the structural basis for peptide bond cleavage via an N --> O or N --> S acyl shift that is used by various groups of intramolecular autoprocessing proteins.

-	~~<!--~~	+	Structural insights into the mechanism of intramolecular proteolysis.,Xu Q, Buckley D, Guan C, Guo HC Cell. 1999 Sep 3;98(5):651-61. PMID:10490104<ref>PMID:10490104</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_9gaa"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_9gaa\| PDB=9gaa \| SCENE= }}~~	+

-	~~===PRECURSOR OF THE T152A MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_10490104}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 10490104 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_10490104}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~9GAA is a 2 chains structure of sequences from [http:~~/~~/en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GAA OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:10490104<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Elizabethkingia meningoseptica]]		[[Category: Elizabethkingia meningoseptica]]
	[[Category: Guo, H C.]]		[[Category: Guo, H C.]]
Line 29:		Line 33:
	[[Category: Autoproteolysis]]		[[Category: Autoproteolysis]]
	[[Category: Glycosylasparaginase]]		[[Category: Glycosylasparaginase]]
		+	[[Category: Hydrolase]]
	[[Category: Mutant]]		[[Category: Mutant]]
	[[Category: N-terminal nucleophile hydrolase]]		[[Category: N-terminal nucleophile hydrolase]]
	[[Category: Precursor]]		[[Category: Precursor]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:32:31 2009''

OCA at 16:32, 17 February 2009

OCA — Tue, 17 Feb 2009 16:32:32 GMT

←Older revision		Revision as of 16:32, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	9GAA is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GAA OCA].	+	9GAA is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GAA OCA].

	==Reference==		==Reference==
-	~~Structural insights into the mechanism of intramolecular proteolysis., Xu Q, Buckley D, Guan C, Guo HC, Cell. 1999 Sep 3;98(5):651-61.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/10490104 10490104]~~	+	<ref group="xtra">PMID:10490104</ref><references group="xtra"/>
	[[Category: Elizabethkingia meningoseptica]]		[[Category: Elizabethkingia meningoseptica]]
-	[[Category: Single protein]]
	[[Category: Guo, H C.]]		[[Category: Guo, H C.]]
	[[Category: Xu, Q.]]		[[Category: Xu, Q.]]
Line 34:		Line 33:
	[[Category: Precursor]]		[[Category: Precursor]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri Jul 11 12~~:56:~~09 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:32:31 2009''

OCA at 09:56, 11 July 2008

OCA — Fri, 11 Jul 2008 09:56:09 GMT

←Older revision		Revision as of 09:56, 11 July 2008
Line 1:		Line 1:
-	[[Image:9gaa.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:9gaa.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_9gaa\| PDB=9gaa \| SCENE= }}		{{STRUCTURE_9gaa\| PDB=9gaa \| SCENE= }}

-	~~'''~~PRECURSOR OF THE T152A MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM~~'''~~	+	===PRECURSOR OF THE T152A MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM===


-	~~==Overview==~~	+	<!--
-	~~A variety of proteins~~, ~~including glycosylasparaginase~~, ~~have recently been found~~ to ~~activate functions by self-catalyzed peptide bond rearrangements from single-chain precursors. Here we present~~ the 1.~~9 A crystal structures of glycosylasparaginase precursors that are able to autoproteolyze via an N --> O acyl shift~~. ~~Several conserved residues are aligned around~~ the ~~scissile peptide bond that is in a highly strained trans peptide bond configuration~~. The structure illustrates how a nucleophilic side chain may attack the scissile peptide bond at the immediate upstream backbone carbonyl and provides an understanding of the structural basis for peptide bond cleavage via an N --~~> O or N --> S acyl shift that is used by various groups of intramolecular autoprocessing proteins.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_10490104}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 10490104 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_10490104}}

	==About this Structure==		==About this Structure==
Line 29:		Line 33:
	[[Category: N-terminal nucleophile hydrolase]]		[[Category: N-terminal nucleophile hydrolase]]
	[[Category: Precursor]]		[[Category: Precursor]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 22~~:52:52 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Jul 11 12:56:09 2008''

OCA at 19:52, 4 May 2008

OCA — Sun, 04 May 2008 19:52:54 GMT

←Older revision		Revision as of 19:52, 4 May 2008
Line 1:		Line 1:
	[[Image:9gaa.gif\|left\|200px]]		[[Image:9gaa.gif\|left\|200px]]

-	~~{{Structure~~	+	<!--
-	~~\|PDB= 9gaa \|SIZE=350\|CAPTION= <scene name='initialview01'>9gaa</scene>~~, ~~resolution 2~~.~~1Å~~	+	The line below this paragraph, containing "STRUCTURE_9gaa", creates the "Structure Box" on the page.
-	~~\|SITE=~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND=~~	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	~~\|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/N~~(4)-(~~beta-N-acetylglucosaminyl~~)~~-L-asparaginase N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase]~~, ~~with EC number [http://www~~.~~brenda~~-~~enzymes.info/php/result_flat.php4?ecno=3.5.1.26 3.5.1.26] </span~~>	+	or leave the SCENE parameter empty for the default display.
-	\|~~GENE~~=	+	-->
-	\|~~DOMAIN~~=	+	{{STRUCTURE_9gaa\| PDB=9gaa \| SCENE= }}
-	~~\|RELATEDENTRY=~~	+
-	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9gaa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9gaa OCA], [http://www.ebi.ac.uk/pdbsum/9gaa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=9gaa RCSB]</span>	+
-	}}	+

	'''PRECURSOR OF THE T152A MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM'''		'''PRECURSOR OF THE T152A MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM'''
Line 24:		Line 21:
	Structural insights into the mechanism of intramolecular proteolysis., Xu Q, Buckley D, Guan C, Guo HC, Cell. 1999 Sep 3;98(5):651-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10490104 10490104]		Structural insights into the mechanism of intramolecular proteolysis., Xu Q, Buckley D, Guan C, Guo HC, Cell. 1999 Sep 3;98(5):651-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10490104 10490104]
	[[Category: Elizabethkingia meningoseptica]]		[[Category: Elizabethkingia meningoseptica]]
-	[[Category: N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase]]
	[[Category: Single protein]]		[[Category: Single protein]]
	[[Category: Guo, H C.]]		[[Category: Guo, H C.]]
	[[Category: Xu, Q.]]		[[Category: Xu, Q.]]
-	[[Category: ~~autoproteolysis~~]]	+	[[Category: Autoproteolysis]]
-	[[Category: ~~glycosylasparaginase~~]]	+	[[Category: Glycosylasparaginase]]
-	[[Category: ~~mutant~~]]	+	[[Category: Mutant]]
-	[[Category: n-terminal nucleophile hydrolase]]	+	[[Category: N-terminal nucleophile hydrolase]]
-	[[Category: ~~precursor~~]]	+	[[Category: Precursor]]
-		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:52:52 2008''
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31 05~~:46:03 2008''	+

OCA at 02:46, 31 March 2008

OCA — Mon, 31 Mar 2008 02:46:04 GMT

←Older revision		Revision as of 02:46, 31 March 2008
Line 5:		Line 5:
	\|SITE=		\|SITE=
	\|LIGAND=		\|LIGAND=
-	\|ACTIVITY= [http://en.wikipedia.org/wiki/N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.26 3.5.1.26]	+	\|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.26 3.5.1.26] </span>
	\|GENE=		\|GENE=
		+	\|DOMAIN=
		+	\|RELATEDENTRY=
		+	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9gaa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9gaa OCA], [http://www.ebi.ac.uk/pdbsum/9gaa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=9gaa RCSB]</span>
	}}		}}

Line 31:		Line 34:
	[[Category: precursor]]		[[Category: precursor]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Mar ~~20 19~~:16:16 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:46:03 2008''