Abrin - Revision history

Karsten Theis at 15:42, 3 September 2020

Karsten Theis — Thu, 03 Sep 2020 15:42:50 GMT

←Older revision		Revision as of 15:42, 3 September 2020
Line 1:		Line 1:
-	<scene name='77/778334/Alpha_chain/1'></scene>
	==Abrin==		==Abrin==
	<StructureSection load='1ABR' size='340' side='right' caption='Glycosylated abrin (PDB code [[1abr]])' scene=''>		<StructureSection load='1ABR' size='340' side='right' caption='Glycosylated abrin (PDB code [[1abr]])' scene=''>

Michal Harel at 08:12, 25 February 2019

Michal Harel — Mon, 25 Feb 2019 08:12:03 GMT

←Older revision		Revision as of 08:12, 25 February 2019
Line 18:		Line 18:
	</StructureSection>		</StructureSection>

-	==3D structures of abrin==
-	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
-
-	[[1abr]], [[5z37]] – IlAbr-A – Indian licorice<br />
-	[[5z3i]] – IlAbr-A + adenine<br />
-	[[5z3j]] – IlAbr-A + nicotinamide<br />
	== References ==		== References ==
	<references/>		<references/>

	[[Category:Topic Page]]		[[Category:Topic Page]]

Michal Harel at 08:10, 25 February 2019

Michal Harel — Mon, 25 Feb 2019 08:10:48 GMT

←Older revision		Revision as of 08:10, 25 February 2019
Line 12:		Line 12:
	== Bioterrorism/Prevention ==		== Bioterrorism/Prevention ==
	Abrin is very similar to ricin because of its structure and functions. While abrin hasn’t been used for bioterrorism, there have been multiple occasions of ricin misuse. For example, in London of 1978, Georgi Markov was attacked by a man with an umbrella, who was employed by the Bulgarian secret service. The umbrella was designed to release a piece ricin which ended up lodging into Markov's skin. Markov died three days after being exposed to the toxin of ricin <ref>"Facts About Abrin.” Centers for Disease Control and Prevention, Centers for Disease Control and Prevention, 18 Nov. 2015, emergency.cdc.gov/agent/abrin/basics/facts.asp.</ref>. Other acts of bioterrorism have included envelopes with the exposure to ricin being mailed to government officials including the President. Since then a vaccine has been created to suppress the effects of ricin poisoning. However, there is still a potential for the use of abrin in a bioterrorism attack. As of right now, they are in development of a vaccine and have learned in one study that a rATB vaccine will possibly rid the symptoms of abrin poisoning<ref>doi:10.1080/21645515.2015.1008879</ref>.		Abrin is very similar to ricin because of its structure and functions. While abrin hasn’t been used for bioterrorism, there have been multiple occasions of ricin misuse. For example, in London of 1978, Georgi Markov was attacked by a man with an umbrella, who was employed by the Bulgarian secret service. The umbrella was designed to release a piece ricin which ended up lodging into Markov's skin. Markov died three days after being exposed to the toxin of ricin <ref>"Facts About Abrin.” Centers for Disease Control and Prevention, Centers for Disease Control and Prevention, 18 Nov. 2015, emergency.cdc.gov/agent/abrin/basics/facts.asp.</ref>. Other acts of bioterrorism have included envelopes with the exposure to ricin being mailed to government officials including the President. Since then a vaccine has been created to suppress the effects of ricin poisoning. However, there is still a potential for the use of abrin in a bioterrorism attack. As of right now, they are in development of a vaccine and have learned in one study that a rATB vaccine will possibly rid the symptoms of abrin poisoning<ref>doi:10.1080/21645515.2015.1008879</ref>.
		+
		+	==3D structures of abrin==
		+	[[Abrin 3D structures]]

	</StructureSection>		</StructureSection>

Jason Telford at 19:02, 7 January 2019

Jason Telford — Mon, 07 Jan 2019 19:02:55 GMT

←Older revision		Revision as of 19:02, 7 January 2019
Line 7:		Line 7:
	Abrin is referred to as a type-2 ribosome-inactivating protein (RIP). The protein contains two chains:<scene name='77/778334/A_chain/1'> α</scene> and <scene name='77/778334/Abrin_b_chain/1'>β</scene> which are connected by a disulfide bond. Both of the chains are <scene name='77/778334/Water/1'>water soluble</scene>. The water molecules hydrolyze the N-C glycosidic bond. The β chain allows the α chain to enter a cell because it attaches to the carbohydrate receptors. The β is composed of the following amino acids: Ile-Val-Glu-Lys-Ser-Lys-Ile-Ser-Ser-Ser-Arg-Tyr-Glu-Pro-Thr. The α chain is known as an N-glycosidase <ref name="Yu">DOI:10.1093/abbs/gmw023</ref>. Once the α chain is in a cell, the chain removes adenine bases and links to the 28S rRNA<ref name="Wooten">DOI: 10.1007/s13181-013-0377-9</ref>. The attachment causes the ribosome to be incompetent when trying to connect to an elongation factor leading to an inhibition of protein synthesis.		Abrin is referred to as a type-2 ribosome-inactivating protein (RIP). The protein contains two chains:<scene name='77/778334/A_chain/1'> α</scene> and <scene name='77/778334/Abrin_b_chain/1'>β</scene> which are connected by a disulfide bond. Both of the chains are <scene name='77/778334/Water/1'>water soluble</scene>. The water molecules hydrolyze the N-C glycosidic bond. The β chain allows the α chain to enter a cell because it attaches to the carbohydrate receptors. The β is composed of the following amino acids: Ile-Val-Glu-Lys-Ser-Lys-Ile-Ser-Ser-Ser-Arg-Tyr-Glu-Pro-Thr. The α chain is known as an N-glycosidase <ref name="Yu">DOI:10.1093/abbs/gmw023</ref>. Once the α chain is in a cell, the chain removes adenine bases and links to the 28S rRNA<ref name="Wooten">DOI: 10.1007/s13181-013-0377-9</ref>. The attachment causes the ribosome to be incompetent when trying to connect to an elongation factor leading to an inhibition of protein synthesis.
	== Medical Potential ==		== Medical Potential ==
-	Abrin has remained a key interest in a treatment for cancer. In one study, scientists wanted to see if abrin would decrease the number of colon cancer cells in vitro and vivo models. One experiment showed that purified abrin can influence cell cycle arrest and apoptosis. The study states abrin “significantly increased p21 mRNA expression and decreased PCNA, cyclin B1, Ki67 mRNA expression" <ref name = "Yu"> which induced a halt in cell growth. For example, p21 can inhibit the CDK2 which regulates the checkpoints of the cell cycle; meaning, if there is an increased level of p21, more CDK2s will be affected. It also stated that abrin can enhance Bcl-2 which causes cytochrome c to be released. With this data, the study suggested that abrin could act as an anticancer treatment for colon cancer; however, a further experiment would have to test abrin’s impact on other cells such as erythrocytes, neurons, or dendritic cells <ref name = "Wooten">.	+	Abrin has remained a key interest in a treatment for cancer. In one study, scientists wanted to see if abrin would decrease the number of colon cancer cells in vitro and vivo models. One experiment showed that purified abrin can influence cell cycle arrest and apoptosis. The study states abrin “significantly increased p21 mRNA expression and decreased PCNA, cyclin B1, Ki67 mRNA expression" <ref name = "Yu"/> which induced a halt in cell growth. For example, p21 can inhibit the CDK2 which regulates the checkpoints of the cell cycle; meaning, if there is an increased level of p21, more CDK2s will be affected. It also stated that abrin can enhance Bcl-2 which causes cytochrome c to be released. With this data, the study suggested that abrin could act as an anticancer treatment for colon cancer; however, a further experiment would have to test abrin’s impact on other cells such as erythrocytes, neurons, or dendritic cells <ref name = "Wooten"/>.
	== Negative Effects/Treatment ==		== Negative Effects/Treatment ==
	If an entire rosary pea is ingested, the person will have mild to no symptoms. However, if the shell breaks, the effects of abrin will be released. Abrin is able to induce apoptosis and cell cycle arrest, ingestion of the protein abrin is lethal. If abrin is consumed or inhaled into the human system, symptoms will include difficulty breathing, vomiting, diarrhea, fever, and organ failure. The lethal dose of abrin is 0.1-1 micrograms <ref> doi:10.12659/AJCR.892917</ref> Someone who has been poisoned by abrin will need fluids, a ventilator, and activated charcoal. Another method for suppressing the symptoms of abrin poisoning is to use CRRT and hemoperfusion. CRRT purifies the blood for a day’s duration. CRRT can destroy toxins up to 1-20kDa in size. Hemoperfusion, which is more effective than CRRT can filter the blood and cause a decrease in levels of the toxin. While these methods have the potential for successfully clearing a toxin, it is still essential for those that have been exposed to a toxin to seek medical help, fast and efficiently<ref>doi:10.1097/MD.0000000000007423</ref>.		If an entire rosary pea is ingested, the person will have mild to no symptoms. However, if the shell breaks, the effects of abrin will be released. Abrin is able to induce apoptosis and cell cycle arrest, ingestion of the protein abrin is lethal. If abrin is consumed or inhaled into the human system, symptoms will include difficulty breathing, vomiting, diarrhea, fever, and organ failure. The lethal dose of abrin is 0.1-1 micrograms <ref> doi:10.12659/AJCR.892917</ref> Someone who has been poisoned by abrin will need fluids, a ventilator, and activated charcoal. Another method for suppressing the symptoms of abrin poisoning is to use CRRT and hemoperfusion. CRRT purifies the blood for a day’s duration. CRRT can destroy toxins up to 1-20kDa in size. Hemoperfusion, which is more effective than CRRT can filter the blood and cause a decrease in levels of the toxin. While these methods have the potential for successfully clearing a toxin, it is still essential for those that have been exposed to a toxin to seek medical help, fast and efficiently<ref>doi:10.1097/MD.0000000000007423</ref>.

Jason Telford at 19:01, 7 January 2019

Jason Telford — Mon, 07 Jan 2019 19:01:28 GMT

←Older revision		Revision as of 19:01, 7 January 2019
Line 7:		Line 7:
	Abrin is referred to as a type-2 ribosome-inactivating protein (RIP). The protein contains two chains:<scene name='77/778334/A_chain/1'> α</scene> and <scene name='77/778334/Abrin_b_chain/1'>β</scene> which are connected by a disulfide bond. Both of the chains are <scene name='77/778334/Water/1'>water soluble</scene>. The water molecules hydrolyze the N-C glycosidic bond. The β chain allows the α chain to enter a cell because it attaches to the carbohydrate receptors. The β is composed of the following amino acids: Ile-Val-Glu-Lys-Ser-Lys-Ile-Ser-Ser-Ser-Arg-Tyr-Glu-Pro-Thr. The α chain is known as an N-glycosidase <ref name="Yu">DOI:10.1093/abbs/gmw023</ref>. Once the α chain is in a cell, the chain removes adenine bases and links to the 28S rRNA<ref name="Wooten">DOI: 10.1007/s13181-013-0377-9</ref>. The attachment causes the ribosome to be incompetent when trying to connect to an elongation factor leading to an inhibition of protein synthesis.		Abrin is referred to as a type-2 ribosome-inactivating protein (RIP). The protein contains two chains:<scene name='77/778334/A_chain/1'> α</scene> and <scene name='77/778334/Abrin_b_chain/1'>β</scene> which are connected by a disulfide bond. Both of the chains are <scene name='77/778334/Water/1'>water soluble</scene>. The water molecules hydrolyze the N-C glycosidic bond. The β chain allows the α chain to enter a cell because it attaches to the carbohydrate receptors. The β is composed of the following amino acids: Ile-Val-Glu-Lys-Ser-Lys-Ile-Ser-Ser-Ser-Arg-Tyr-Glu-Pro-Thr. The α chain is known as an N-glycosidase <ref name="Yu">DOI:10.1093/abbs/gmw023</ref>. Once the α chain is in a cell, the chain removes adenine bases and links to the 28S rRNA<ref name="Wooten">DOI: 10.1007/s13181-013-0377-9</ref>. The attachment causes the ribosome to be incompetent when trying to connect to an elongation factor leading to an inhibition of protein synthesis.
	== Medical Potential ==		== Medical Potential ==
-	Abrin has remained a key interest in a treatment for cancer. In one study, scientists wanted to see if abrin would decrease the number of colon cancer cells in vitro and vivo models. One experiment showed that purified abrin can influence cell cycle arrest and apoptosis. The study states abrin “significantly increased p21 mRNA expression and decreased PCNA, cyclin B1, Ki67 mRNA expression <ref name = "Yu"> which induced a halt in cell growth. For example, p21 can inhibit the CDK2 which regulates the checkpoints of the cell cycle; meaning, if there is an increased level of p21, more CDK2s will be affected. It also stated that abrin can enhance Bcl-2 which causes cytochrome c to be released. With this data, the study suggested that abrin could act as an anticancer treatment for colon cancer; however, a further experiment would have to test abrin’s impact on other cells such as erythrocytes, neurons, or dendritic cells <ref name="Wooten">.	+	Abrin has remained a key interest in a treatment for cancer. In one study, scientists wanted to see if abrin would decrease the number of colon cancer cells in vitro and vivo models. One experiment showed that purified abrin can influence cell cycle arrest and apoptosis. The study states abrin “significantly increased p21 mRNA expression and decreased PCNA, cyclin B1, Ki67 mRNA expression" <ref name = "Yu"> which induced a halt in cell growth. For example, p21 can inhibit the CDK2 which regulates the checkpoints of the cell cycle; meaning, if there is an increased level of p21, more CDK2s will be affected. It also stated that abrin can enhance Bcl-2 which causes cytochrome c to be released. With this data, the study suggested that abrin could act as an anticancer treatment for colon cancer; however, a further experiment would have to test abrin’s impact on other cells such as erythrocytes, neurons, or dendritic cells <ref name = "Wooten">.
	== Negative Effects/Treatment ==		== Negative Effects/Treatment ==
	If an entire rosary pea is ingested, the person will have mild to no symptoms. However, if the shell breaks, the effects of abrin will be released. Abrin is able to induce apoptosis and cell cycle arrest, ingestion of the protein abrin is lethal. If abrin is consumed or inhaled into the human system, symptoms will include difficulty breathing, vomiting, diarrhea, fever, and organ failure. The lethal dose of abrin is 0.1-1 micrograms <ref> doi:10.12659/AJCR.892917</ref> Someone who has been poisoned by abrin will need fluids, a ventilator, and activated charcoal. Another method for suppressing the symptoms of abrin poisoning is to use CRRT and hemoperfusion. CRRT purifies the blood for a day’s duration. CRRT can destroy toxins up to 1-20kDa in size. Hemoperfusion, which is more effective than CRRT can filter the blood and cause a decrease in levels of the toxin. While these methods have the potential for successfully clearing a toxin, it is still essential for those that have been exposed to a toxin to seek medical help, fast and efficiently<ref>doi:10.1097/MD.0000000000007423</ref>.		If an entire rosary pea is ingested, the person will have mild to no symptoms. However, if the shell breaks, the effects of abrin will be released. Abrin is able to induce apoptosis and cell cycle arrest, ingestion of the protein abrin is lethal. If abrin is consumed or inhaled into the human system, symptoms will include difficulty breathing, vomiting, diarrhea, fever, and organ failure. The lethal dose of abrin is 0.1-1 micrograms <ref> doi:10.12659/AJCR.892917</ref> Someone who has been poisoned by abrin will need fluids, a ventilator, and activated charcoal. Another method for suppressing the symptoms of abrin poisoning is to use CRRT and hemoperfusion. CRRT purifies the blood for a day’s duration. CRRT can destroy toxins up to 1-20kDa in size. Hemoperfusion, which is more effective than CRRT can filter the blood and cause a decrease in levels of the toxin. While these methods have the potential for successfully clearing a toxin, it is still essential for those that have been exposed to a toxin to seek medical help, fast and efficiently<ref>doi:10.1097/MD.0000000000007423</ref>.

Jason Telford at 18:59, 7 January 2019

Jason Telford — Mon, 07 Jan 2019 18:59:13 GMT

←Older revision		Revision as of 18:59, 7 January 2019
Line 7:		Line 7:
	Abrin is referred to as a type-2 ribosome-inactivating protein (RIP). The protein contains two chains:<scene name='77/778334/A_chain/1'> α</scene> and <scene name='77/778334/Abrin_b_chain/1'>β</scene> which are connected by a disulfide bond. Both of the chains are <scene name='77/778334/Water/1'>water soluble</scene>. The water molecules hydrolyze the N-C glycosidic bond. The β chain allows the α chain to enter a cell because it attaches to the carbohydrate receptors. The β is composed of the following amino acids: Ile-Val-Glu-Lys-Ser-Lys-Ile-Ser-Ser-Ser-Arg-Tyr-Glu-Pro-Thr. The α chain is known as an N-glycosidase <ref name="Yu">DOI:10.1093/abbs/gmw023</ref>. Once the α chain is in a cell, the chain removes adenine bases and links to the 28S rRNA<ref name="Wooten">DOI: 10.1007/s13181-013-0377-9</ref>. The attachment causes the ribosome to be incompetent when trying to connect to an elongation factor leading to an inhibition of protein synthesis.		Abrin is referred to as a type-2 ribosome-inactivating protein (RIP). The protein contains two chains:<scene name='77/778334/A_chain/1'> α</scene> and <scene name='77/778334/Abrin_b_chain/1'>β</scene> which are connected by a disulfide bond. Both of the chains are <scene name='77/778334/Water/1'>water soluble</scene>. The water molecules hydrolyze the N-C glycosidic bond. The β chain allows the α chain to enter a cell because it attaches to the carbohydrate receptors. The β is composed of the following amino acids: Ile-Val-Glu-Lys-Ser-Lys-Ile-Ser-Ser-Ser-Arg-Tyr-Glu-Pro-Thr. The α chain is known as an N-glycosidase <ref name="Yu">DOI:10.1093/abbs/gmw023</ref>. Once the α chain is in a cell, the chain removes adenine bases and links to the 28S rRNA<ref name="Wooten">DOI: 10.1007/s13181-013-0377-9</ref>. The attachment causes the ribosome to be incompetent when trying to connect to an elongation factor leading to an inhibition of protein synthesis.
	== Medical Potential ==		== Medical Potential ==
-	Abrin has remained a key interest in a treatment for cancer. In one study, scientists wanted to see if abrin would decrease the number of colon cancer cells in vitro and vivo models. One experiment showed that purified abrin can influence cell cycle arrest and apoptosis. The study states abrin “significantly increased p21 mRNA expression and decreased PCNA, cyclin B1, Ki67 mRNA ~~expression”~~ <ref name = "Yu"> which induced a halt in cell growth. For example, p21 can inhibit the CDK2 which regulates the checkpoints of the cell cycle; meaning, if there is an increased level of p21, more CDK2s will be affected. It also stated that abrin can enhance Bcl-2 which causes cytochrome c to be released. With this data, the study suggested that abrin could act as an anticancer treatment for colon cancer; however, a further experiment would have to test abrin’s impact on other cells such as erythrocytes, neurons, or dendritic cells<ref name="Wooten">.	+	Abrin has remained a key interest in a treatment for cancer. In one study, scientists wanted to see if abrin would decrease the number of colon cancer cells in vitro and vivo models. One experiment showed that purified abrin can influence cell cycle arrest and apoptosis. The study states abrin “significantly increased p21 mRNA expression and decreased PCNA, cyclin B1, Ki67 mRNA expression <ref name = "Yu"> which induced a halt in cell growth. For example, p21 can inhibit the CDK2 which regulates the checkpoints of the cell cycle; meaning, if there is an increased level of p21, more CDK2s will be affected. It also stated that abrin can enhance Bcl-2 which causes cytochrome c to be released. With this data, the study suggested that abrin could act as an anticancer treatment for colon cancer; however, a further experiment would have to test abrin’s impact on other cells such as erythrocytes, neurons, or dendritic cells <ref name="Wooten">.
	== Negative Effects/Treatment ==		== Negative Effects/Treatment ==
	If an entire rosary pea is ingested, the person will have mild to no symptoms. However, if the shell breaks, the effects of abrin will be released. Abrin is able to induce apoptosis and cell cycle arrest, ingestion of the protein abrin is lethal. If abrin is consumed or inhaled into the human system, symptoms will include difficulty breathing, vomiting, diarrhea, fever, and organ failure. The lethal dose of abrin is 0.1-1 micrograms <ref> doi:10.12659/AJCR.892917</ref> Someone who has been poisoned by abrin will need fluids, a ventilator, and activated charcoal. Another method for suppressing the symptoms of abrin poisoning is to use CRRT and hemoperfusion. CRRT purifies the blood for a day’s duration. CRRT can destroy toxins up to 1-20kDa in size. Hemoperfusion, which is more effective than CRRT can filter the blood and cause a decrease in levels of the toxin. While these methods have the potential for successfully clearing a toxin, it is still essential for those that have been exposed to a toxin to seek medical help, fast and efficiently<ref>doi:10.1097/MD.0000000000007423</ref>.		If an entire rosary pea is ingested, the person will have mild to no symptoms. However, if the shell breaks, the effects of abrin will be released. Abrin is able to induce apoptosis and cell cycle arrest, ingestion of the protein abrin is lethal. If abrin is consumed or inhaled into the human system, symptoms will include difficulty breathing, vomiting, diarrhea, fever, and organ failure. The lethal dose of abrin is 0.1-1 micrograms <ref> doi:10.12659/AJCR.892917</ref> Someone who has been poisoned by abrin will need fluids, a ventilator, and activated charcoal. Another method for suppressing the symptoms of abrin poisoning is to use CRRT and hemoperfusion. CRRT purifies the blood for a day’s duration. CRRT can destroy toxins up to 1-20kDa in size. Hemoperfusion, which is more effective than CRRT can filter the blood and cause a decrease in levels of the toxin. While these methods have the potential for successfully clearing a toxin, it is still essential for those that have been exposed to a toxin to seek medical help, fast and efficiently<ref>doi:10.1097/MD.0000000000007423</ref>.

Jason Telford at 18:54, 7 January 2019

Jason Telford — Mon, 07 Jan 2019 18:54:17 GMT

←Older revision		Revision as of 18:54, 7 January 2019
Line 5:		Line 5:
	''Abrus precatorius'' (or rosary peas) contain the protein '''abrin'''. Rosary peas are red and oval-shaped with a black edge towards one end. In some cultures, they are used to make beaded jewelry. Abrin can exhibit beneficial uses for medical research, but it can also be destructive if it directly enters an organism’s body because of its toxic properties.		''Abrus precatorius'' (or rosary peas) contain the protein '''abrin'''. Rosary peas are red and oval-shaped with a black edge towards one end. In some cultures, they are used to make beaded jewelry. Abrin can exhibit beneficial uses for medical research, but it can also be destructive if it directly enters an organism’s body because of its toxic properties.
	== Structure ==		== Structure ==
-	Abrin is referred to as a type-2 ribosome-inactivating protein (RIP). The protein contains two chains:<scene name='77/778334/A_chain/1'> α</scene> and <scene name='77/778334/Abrin_b_chain/1'>β</scene> which are connected by a disulfide bond. Both of the chains are <scene name='77/778334/Water/1'>water soluble</scene>. The water molecules hydrolyze the N-C glycosidic bond. The β chain allows the α chain to enter a cell because it attaches to the carbohydrate receptors. The β is composed of the following amino acids: Ile-Val-Glu-Lys-Ser-Lys-Ile-Ser-Ser-Ser-Arg-Tyr-Glu-Pro-Thr. The α chain is known as an N-glycosidase <ref>DOI:10.1093/abbs/gmw023</ref>. Once the α chain is in a cell, the chain removes adenine bases and links to the 28S rRNA<ref>DOI: 10.1007/s13181-013-0377-9</ref>. The attachment causes the ribosome to be incompetent when trying to connect to an elongation factor leading to an inhibition of protein synthesis.	+	Abrin is referred to as a type-2 ribosome-inactivating protein (RIP). The protein contains two chains:<scene name='77/778334/A_chain/1'> α</scene> and <scene name='77/778334/Abrin_b_chain/1'>β</scene> which are connected by a disulfide bond. Both of the chains are <scene name='77/778334/Water/1'>water soluble</scene>. The water molecules hydrolyze the N-C glycosidic bond. The β chain allows the α chain to enter a cell because it attaches to the carbohydrate receptors. The β is composed of the following amino acids: Ile-Val-Glu-Lys-Ser-Lys-Ile-Ser-Ser-Ser-Arg-Tyr-Glu-Pro-Thr. The α chain is known as an N-glycosidase <ref name="Yu">DOI:10.1093/abbs/gmw023</ref>. Once the α chain is in a cell, the chain removes adenine bases and links to the 28S rRNA<ref name="Wooten">DOI: 10.1007/s13181-013-0377-9</ref>. The attachment causes the ribosome to be incompetent when trying to connect to an elongation factor leading to an inhibition of protein synthesis.
	== Medical Potential ==		== Medical Potential ==
-	Abrin has remained a key interest in a treatment for cancer. In one study, scientists wanted to see if abrin would decrease the number of colon cancer cells in vitro and vivo models. One experiment showed that purified abrin can influence cell cycle arrest and apoptosis. The study states abrin “significantly increased p21 mRNA expression and decreased PCNA, cyclin B1, Ki67 mRNA expression” <ref~~>DOI:10.1093/abbs/gmw023</ref~~> which induced a halt in cell growth. For example, p21 can inhibit the CDK2 which regulates the checkpoints of the cell cycle; meaning, if there is an increased level of p21, more CDK2s will be affected. It also stated that abrin can enhance Bcl-2 which causes cytochrome c to be released. With this data, the study suggested that abrin could act as an anticancer treatment for colon cancer; however, a further experiment would have to test abrin’s impact on other cells such as erythrocytes, neurons, or dendritic cells<ref~~>DOI: 10.1007/s13181-013-0377-9</ref~~>.	+	Abrin has remained a key interest in a treatment for cancer. In one study, scientists wanted to see if abrin would decrease the number of colon cancer cells in vitro and vivo models. One experiment showed that purified abrin can influence cell cycle arrest and apoptosis. The study states abrin “significantly increased p21 mRNA expression and decreased PCNA, cyclin B1, Ki67 mRNA expression” <ref name = "Yu"> which induced a halt in cell growth. For example, p21 can inhibit the CDK2 which regulates the checkpoints of the cell cycle; meaning, if there is an increased level of p21, more CDK2s will be affected. It also stated that abrin can enhance Bcl-2 which causes cytochrome c to be released. With this data, the study suggested that abrin could act as an anticancer treatment for colon cancer; however, a further experiment would have to test abrin’s impact on other cells such as erythrocytes, neurons, or dendritic cells<ref name="Wooten">.
	== Negative Effects/Treatment ==		== Negative Effects/Treatment ==
	If an entire rosary pea is ingested, the person will have mild to no symptoms. However, if the shell breaks, the effects of abrin will be released. Abrin is able to induce apoptosis and cell cycle arrest, ingestion of the protein abrin is lethal. If abrin is consumed or inhaled into the human system, symptoms will include difficulty breathing, vomiting, diarrhea, fever, and organ failure. The lethal dose of abrin is 0.1-1 micrograms <ref> doi:10.12659/AJCR.892917</ref> Someone who has been poisoned by abrin will need fluids, a ventilator, and activated charcoal. Another method for suppressing the symptoms of abrin poisoning is to use CRRT and hemoperfusion. CRRT purifies the blood for a day’s duration. CRRT can destroy toxins up to 1-20kDa in size. Hemoperfusion, which is more effective than CRRT can filter the blood and cause a decrease in levels of the toxin. While these methods have the potential for successfully clearing a toxin, it is still essential for those that have been exposed to a toxin to seek medical help, fast and efficiently<ref>doi:10.1097/MD.0000000000007423</ref>.		If an entire rosary pea is ingested, the person will have mild to no symptoms. However, if the shell breaks, the effects of abrin will be released. Abrin is able to induce apoptosis and cell cycle arrest, ingestion of the protein abrin is lethal. If abrin is consumed or inhaled into the human system, symptoms will include difficulty breathing, vomiting, diarrhea, fever, and organ failure. The lethal dose of abrin is 0.1-1 micrograms <ref> doi:10.12659/AJCR.892917</ref> Someone who has been poisoned by abrin will need fluids, a ventilator, and activated charcoal. Another method for suppressing the symptoms of abrin poisoning is to use CRRT and hemoperfusion. CRRT purifies the blood for a day’s duration. CRRT can destroy toxins up to 1-20kDa in size. Hemoperfusion, which is more effective than CRRT can filter the blood and cause a decrease in levels of the toxin. While these methods have the potential for successfully clearing a toxin, it is still essential for those that have been exposed to a toxin to seek medical help, fast and efficiently<ref>doi:10.1097/MD.0000000000007423</ref>.

Michal Harel at 07:40, 2 January 2019

Michal Harel — Wed, 02 Jan 2019 07:40:04 GMT

←Older revision		Revision as of 07:40, 2 January 2019
Line 1:		Line 1:
	<scene name='77/778334/Alpha_chain/1'></scene>		<scene name='77/778334/Alpha_chain/1'></scene>
	==Abrin==		==Abrin==
-	<StructureSection load='1ABR' size='340' side='right' caption='~~Caption for this structure~~' scene=''>	+	<StructureSection load='1ABR' size='340' side='right' caption='Glycosylated abrin (PDB code [[1abr]])' scene=''>
	== Introduction ==		== Introduction ==
-	Abrus precatorius (or rosary peas) contain the protein abrin. Rosary peas are red and oval-shaped with a black edge towards one end. In some cultures, they are used to make beaded jewelry. Abrin can exhibit beneficial uses for medical research, but it can also be destructive if it directly enters an organism’s body because of its toxic properties.	+	''Abrus precatorius'' (or rosary peas) contain the protein '''abrin'''. Rosary peas are red and oval-shaped with a black edge towards one end. In some cultures, they are used to make beaded jewelry. Abrin can exhibit beneficial uses for medical research, but it can also be destructive if it directly enters an organism’s body because of its toxic properties.
	== Structure ==		== Structure ==
	Abrin is referred to as a type-2 ribosome-inactivating protein (RIP). The protein contains two chains:<scene name='77/778334/A_chain/1'> α</scene> and <scene name='77/778334/Abrin_b_chain/1'>β</scene> which are connected by a disulfide bond. Both of the chains are <scene name='77/778334/Water/1'>water soluble</scene>. The water molecules hydrolyze the N-C glycosidic bond. The β chain allows the α chain to enter a cell because it attaches to the carbohydrate receptors. The β is composed of the following amino acids: Ile-Val-Glu-Lys-Ser-Lys-Ile-Ser-Ser-Ser-Arg-Tyr-Glu-Pro-Thr. The α chain is known as an N-glycosidase <ref>DOI:10.1093/abbs/gmw023</ref>. Once the α chain is in a cell, the chain removes adenine bases and links to the 28S rRNA<ref>DOI: 10.1007/s13181-013-0377-9</ref>. The attachment causes the ribosome to be incompetent when trying to connect to an elongation factor leading to an inhibition of protein synthesis.		Abrin is referred to as a type-2 ribosome-inactivating protein (RIP). The protein contains two chains:<scene name='77/778334/A_chain/1'> α</scene> and <scene name='77/778334/Abrin_b_chain/1'>β</scene> which are connected by a disulfide bond. Both of the chains are <scene name='77/778334/Water/1'>water soluble</scene>. The water molecules hydrolyze the N-C glycosidic bond. The β chain allows the α chain to enter a cell because it attaches to the carbohydrate receptors. The β is composed of the following amino acids: Ile-Val-Glu-Lys-Ser-Lys-Ile-Ser-Ser-Ser-Arg-Tyr-Glu-Pro-Thr. The α chain is known as an N-glycosidase <ref>DOI:10.1093/abbs/gmw023</ref>. Once the α chain is in a cell, the chain removes adenine bases and links to the 28S rRNA<ref>DOI: 10.1007/s13181-013-0377-9</ref>. The attachment causes the ribosome to be incompetent when trying to connect to an elongation factor leading to an inhibition of protein synthesis.
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	</StructureSection>		</StructureSection>
		+
		+	==3D structures of abrin==
		+	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
		+
		+	[[1abr]], [[5z37]] – IlAbr-A – Indian licorice<br />
		+	[[5z3i]] – IlAbr-A + adenine<br />
		+	[[5z3j]] – IlAbr-A + nicotinamide<br />
	== References ==		== References ==
	<references/>		<references/>
		+
		+	[[Category:Topic Page]]

Emily Street at 19:20, 2 May 2018

Emily Street — Wed, 02 May 2018 19:20:40 GMT

←Older revision		Revision as of 19:20, 2 May 2018
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-	<scene name='77/778334/Alpha_chain/1'>~~Text To Be Displayed~~</scene>	+	<scene name='77/778334/Alpha_chain/1'></scene>
	==Abrin==		==Abrin==
	<StructureSection load='1ABR' size='340' side='right' caption='Caption for this structure' scene=''>		<StructureSection load='1ABR' size='340' side='right' caption='Caption for this structure' scene=''>

Emily Street at 19:20, 2 May 2018

Emily Street — Wed, 02 May 2018 19:20:24 GMT

←Older revision		Revision as of 19:20, 2 May 2018
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-		+	<scene name='77/778334/Alpha_chain/1'>Text To Be Displayed</scene>
-	<scene name='77/778334/Alpha_chain/1'>Text To Be Displayed</scene~~>{{Sandbox_Reserved_Telford2018}}<!-- PLEASE ADD YOUR CONTENT BELOW HERE --~~>	+
	==Abrin==		==Abrin==
	<StructureSection load='1ABR' size='340' side='right' caption='Caption for this structure' scene=''>		<StructureSection load='1ABR' size='340' side='right' caption='Caption for this structure' scene=''>