Alice Clark/BRCT - Revision history

Michal Harel at 10:47, 27 January 2022

Michal Harel — Thu, 27 Jan 2022 10:47:01 GMT

←Older revision		Revision as of 10:47, 27 January 2022
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	<scene name='86/868791/Rainbow_veiw/1'>test rainbow</scene>		<scene name='86/868791/Rainbow_veiw/1'>test rainbow</scene>
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		+	===3D structures of MC4R===
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		+	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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		+	[[6w25]] – hMC4R + peptide – human<br />
		+	[[7aue]], [[7f55]], [[7f58]] – hMC4R in Gs protein complex + nanobody – Cryo EM<br />
		+	[[7piu]] – hMC4R in Gs protein complex + nanobody + drug – Cryo EM<br />
		+	[[7f53]], [[7piu]], [[7piv]] – hMC4R in Gs protein complex + nanobody + a-melanocyte-stimulating hormone – Cryo EM<br />
		+	[[Category:Topic Page]]
		+
		+
		+
		+
		+

Michal Harel at 10:16, 27 January 2022

Michal Harel — Thu, 27 Jan 2022 10:16:36 GMT

←Older revision		Revision as of 10:16, 27 January 2022
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-	<Structure load='6w25' size='350' frame='true' align='right' caption='Melanocrtin 4 Receptor' scene='86/868791/Alices_rainbow_selection/1' />	+	<Structure load='6w25' size='350' frame='true' align='right' caption='Melanocrtin 4 Receptor complex with peptide (PDB code [[6w25]])' scene='86/868791/Alices_rainbow_selection/1' />

	[[Image:Screenshot_2021-02-25_at_15.36.50.png\|350px\|right\|thumb\| MCR1]]		[[Image:Screenshot_2021-02-25_at_15.36.50.png\|350px\|right\|thumb\| MCR1]]
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	==Melanocortin 1 Receptor: An insight to MCR1 structure, function and regulation==		==Melanocortin 1 Receptor: An insight to MCR1 structure, function and regulation==
	===Introduction to MC1R===		===Introduction to MC1R===
-	The highly polymorphic human MC1R gene, located on chromosome 16q24.3 encodes for a MCR1 receptor also known as melanocyte -stimulating hormone receptor. The melanocortin receptor family consists of five members: MC1R, MCR2, MCR3, MC4R and MC5R all of which exhibit functions and are expressed differently (Wolf Horrell, Boulanger and D’Orazio, 2016). The gene is expressed in melanocytes along with other cell types that inhabit the skin such as keratinocytes and fibroblast as well as cells that operate the immune system (Gruis and Doorn, 2012). The activation of the MC1R receptor by ultraviolet radiation increases the production of the dark eumelanin pigment, resulting in the darkening of the skin. MCR1 stimulation also results in increased melanocyte dendricity, proliferation, cell survival and DNA repair. The loss of melanocortin receptor function results in the production of the red/yellow pheomelanin pigment by melanocytes, resulting in the red hair, fair skin, poor tanning, freckling and increased skin cancer risk in humans (Beaumont et al., 2011).	+	The highly polymorphic human MC1R gene, located on chromosome 16q24.3 encodes for a MCR1 receptor also known as melanocyte -stimulating hormone receptor. The '''melanocortin receptor''' family consists of five members: MC1R, MCR2, MCR3, MC4R and MC5R all of which exhibit functions and are expressed differently (Wolf Horrell, Boulanger and D’Orazio, 2016). The gene is expressed in melanocytes along with other cell types that inhabit the skin such as keratinocytes and fibroblast as well as cells that operate the immune system (Gruis and Doorn, 2012). The activation of the MC1R receptor by ultraviolet radiation increases the production of the dark eumelanin pigment, resulting in the darkening of the skin. MCR1 stimulation also results in increased melanocyte dendricity, proliferation, cell survival and DNA repair. The loss of melanocortin receptor function results in the production of the red/yellow pheomelanin pigment by melanocytes, resulting in the red hair, fair skin, poor tanning, freckling and increased skin cancer risk in humans (Beaumont et al., 2011).
	===Determination of MC1R Structure===		===Determination of MC1R Structure===
	The mature MC1R protein is made up of 317 amino acids and a 7 a-helical transmembrane domain. Based on the sequence similarity analysis, the melanocortin receptor family belong to the class A of G-coupled protein receptors therefore direct information on their secondary and tertiary structures is limited as G coupled protein receptors are resistant to crystallisation (Garcia-Borron, Sanchez-Laorden and Jimenez-Cervantes, 2005) (Zhao and Wu, 2012).		The mature MC1R protein is made up of 317 amino acids and a 7 a-helical transmembrane domain. Based on the sequence similarity analysis, the melanocortin receptor family belong to the class A of G-coupled protein receptors therefore direct information on their secondary and tertiary structures is limited as G coupled protein receptors are resistant to crystallisation (Garcia-Borron, Sanchez-Laorden and Jimenez-Cervantes, 2005) (Zhao and Wu, 2012).

Alice Clark at 14:38, 10 March 2021

Alice Clark — Wed, 10 Mar 2021 14:38:48 GMT

←Older revision		Revision as of 14:38, 10 March 2021
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	<Structure load='6w25' size='350' frame='true' align='right' caption='Melanocrtin 4 Receptor' scene='86/868791/Alices_rainbow_selection/1' />		<Structure load='6w25' size='350' frame='true' align='right' caption='Melanocrtin 4 Receptor' scene='86/868791/Alices_rainbow_selection/1' />

-	[Image:Screenshot_2021-02-25_at_15.36.50.png\|350px\|right\|thumb\| MCR1]]	+	[[Image:Screenshot_2021-02-25_at_15.36.50.png\|350px\|right\|thumb\| MCR1]]

	==Melanocortin 1 Receptor: An insight to MCR1 structure, function and regulation==		==Melanocortin 1 Receptor: An insight to MCR1 structure, function and regulation==

Alice Clark at 14:37, 10 March 2021

Alice Clark — Wed, 10 Mar 2021 14:37:57 GMT

←Older revision		Revision as of 14:37, 10 March 2021
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	<Structure load='6w25' size='350' frame='true' align='right' caption='Melanocrtin 4 Receptor' scene='86/868791/Alices_rainbow_selection/1' />		<Structure load='6w25' size='350' frame='true' align='right' caption='Melanocrtin 4 Receptor' scene='86/868791/Alices_rainbow_selection/1' />

-	~~[size='350'~~ [Image:Screenshot_2021-02-25_at_15.36.50.png]]	+	[Image:Screenshot_2021-02-25_at_15.36.50.png\|350px\|right\|thumb\| MCR1]]

	==Melanocortin 1 Receptor: An insight to MCR1 structure, function and regulation==		==Melanocortin 1 Receptor: An insight to MCR1 structure, function and regulation==

Alice Clark at 14:23, 10 March 2021

Alice Clark — Wed, 10 Mar 2021 14:23:12 GMT

←Older revision		Revision as of 14:23, 10 March 2021
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	<Structure load='6w25' size='350' frame='true' align='right' caption='Melanocrtin 4 Receptor' scene='86/868791/Alices_rainbow_selection/1' />		<Structure load='6w25' size='350' frame='true' align='right' caption='Melanocrtin 4 Receptor' scene='86/868791/Alices_rainbow_selection/1' />

-	[[Image:Screenshot_2021-02-25_at_15.36.50.png]]	+	[size='350' [Image:Screenshot_2021-02-25_at_15.36.50.png]]

	==Melanocortin 1 Receptor: An insight to MCR1 structure, function and regulation==		==Melanocortin 1 Receptor: An insight to MCR1 structure, function and regulation==

Alice Clark at 14:22, 10 March 2021

Alice Clark — Wed, 10 Mar 2021 14:22:08 GMT

←Older revision		Revision as of 14:22, 10 March 2021
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	<Structure load='6w25' size='350' frame='true' align='right' caption='Melanocrtin 4 Receptor' scene='86/868791/Alices_rainbow_selection/1' />		<Structure load='6w25' size='350' frame='true' align='right' caption='Melanocrtin 4 Receptor' scene='86/868791/Alices_rainbow_selection/1' />

		+	[[Image:Screenshot_2021-02-25_at_15.36.50.png]]

	==Melanocortin 1 Receptor: An insight to MCR1 structure, function and regulation==		==Melanocortin 1 Receptor: An insight to MCR1 structure, function and regulation==

Alice Clark at 14:11, 10 March 2021

Alice Clark — Wed, 10 Mar 2021 14:11:39 GMT

←Older revision		Revision as of 14:11, 10 March 2021
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-	<Structure load='6w25' size='350' frame='true' align='right' caption='Melanocrtin 4 Receptor' scene ~~name~~='86/868791/Alices_rainbow_selection/1' />	+	<Structure load='6w25' size='350' frame='true' align='right' caption='Melanocrtin 4 Receptor' scene='86/868791/Alices_rainbow_selection/1' />

Alice Clark at 14:08, 10 March 2021

Alice Clark — Wed, 10 Mar 2021 14:08:31 GMT

←Older revision		Revision as of 14:08, 10 March 2021
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-	<Structure load='6w25' size='350' frame='true' align='right' caption='Melanocrtin 4 Receptor' scene name='86/868791/~~Rainbow_veiw~~/1~~'>test</scene~~' />	+	<Structure load='6w25' size='350' frame='true' align='right' caption='Melanocrtin 4 Receptor' scene name='86/868791/Alices_rainbow_selection/1' />

Alice Clark at 14:03, 10 March 2021

Alice Clark — Wed, 10 Mar 2021 14:03:38 GMT

←Older revision		Revision as of 14:03, 10 March 2021
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-	<Structure load='6w25' size='350' frame='true' align='right' caption='Melanocrtin 4 Receptor' ~~scene='<~~scene name='86/868791/Rainbow_veiw/1'>~~TextToBeDisplayed~~</scene>' />	+	<Structure load='6w25' size='350' frame='true' align='right' caption='Melanocrtin 4 Receptor' scene name='86/868791/Rainbow_veiw/1'>test</scene' />

Alice Clark: Undo revision 3364849 by Alice Clark (Talk)

Alice Clark — Wed, 10 Mar 2021 14:02:10 GMT

Undo revision 3364849 by Alice Clark (Talk)

←Older revision		Revision as of 14:02, 10 March 2021
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	Ligands		Ligands

		+	<scene name='86/868791/Rainbow_veiw/1'>test rainbow</scene>