Bromelain - Revision history

Ann Taylor at 03:43, 16 April 2019

Ann Taylor — Tue, 16 Apr 2019 03:43:27 GMT

←Older revision		Revision as of 03:43, 16 April 2019
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	==Temperature stability==		==Temperature stability==
	At the optimum temperature, the enzyme acts the fastest, but (at least the fruit variant) is destroyed within few minutes. After an hour at 50 °C (122 °F), 83% of the enzyme remains, while at 40 °C (104 °F), practically 100% remains. As a result of this, the optimum temperature for maximum cumulated activity over time is 35-45 °C. At room temperature, the enzyme can survive at least a week even under multiple freeze-thaw cycles.		At the optimum temperature, the enzyme acts the fastest, but (at least the fruit variant) is destroyed within few minutes. After an hour at 50 °C (122 °F), 83% of the enzyme remains, while at 40 °C (104 °F), practically 100% remains. As a result of this, the optimum temperature for maximum cumulated activity over time is 35-45 °C. At room temperature, the enzyme can survive at least a week even under multiple freeze-thaw cycles.
		+
		+	==How it works==
		+	Bromelain is a cysteine protease. The <scene name='60/609840/Active_site/1'>active site</scene> contains a cysteine residue. A neighboring histidine increases the ionization of the cysteine, making it a better nucleophile.

	== 3D Structures of bromelain ==		== 3D Structures of bromelain ==

Michal Harel at 07:48, 5 January 2017

Michal Harel — Thu, 05 Jan 2017 07:48:10 GMT

←Older revision		Revision as of 07:48, 5 January 2017
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	At the optimum temperature, the enzyme acts the fastest, but (at least the fruit variant) is destroyed within few minutes. After an hour at 50 °C (122 °F), 83% of the enzyme remains, while at 40 °C (104 °F), practically 100% remains. As a result of this, the optimum temperature for maximum cumulated activity over time is 35-45 °C. At room temperature, the enzyme can survive at least a week even under multiple freeze-thaw cycles.		At the optimum temperature, the enzyme acts the fastest, but (at least the fruit variant) is destroyed within few minutes. After an hour at 50 °C (122 °F), 83% of the enzyme remains, while at 40 °C (104 °F), practically 100% remains. As a result of this, the optimum temperature for maximum cumulated activity over time is 35-45 °C. At room temperature, the enzyme can survive at least a week even under multiple freeze-thaw cycles.

-	== 3D Structures of ~~lactoferrin~~ ==	+	== 3D Structures of bromelain ==

	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}		Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}

-	[[1w0q]] -	+	[[1w0q]] - pineapple - model<br />
	== References ==		== References ==
	<references/>		<references/>

Michal Harel at 07:47, 5 January 2017

Michal Harel — Thu, 05 Jan 2017 07:47:09 GMT

←Older revision		Revision as of 07:47, 5 January 2017
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	<StructureSection load='1w0q' size='350' side='right' scene='' caption='Pineapple bromelain (PDB code [[1w0q]])'>		<StructureSection load='1w0q' size='350' side='right' scene='' caption='Pineapple bromelain (PDB code [[1w0q]])'>
-	Bromelain is a protein extract derived from the stems of pineapples.The extract has a history of folk medicine use. As a culinary ingredient, it may be used as a meat tenderizer.	+	'''Bromelain''' is a protein extract derived from the stems of pineapples.The extract has a history of folk medicine use. As a culinary ingredient, it may be used as a meat tenderizer.
	Bromelain may refer to either of two protease enzymes extracted from the plants of the family Bromeliaceae, or it may refer to a combination of those enzymes along with other compounds produced in an extract.		Bromelain may refer to either of two protease enzymes extracted from the plants of the family Bromeliaceae, or it may refer to a combination of those enzymes along with other compounds produced in an extract.
	Although tested in a variety of research models for its possible efficacy against diseases, bromelain has no established research evidence as a nutraceutical or drug.Limited evidence exists for possible effectiveness of a product used to reduce pain from osteoarthritis that combines bromelain with trypsin and rutin.		Although tested in a variety of research models for its possible efficacy against diseases, bromelain has no established research evidence as a nutraceutical or drug.Limited evidence exists for possible effectiveness of a product used to reduce pain from osteoarthritis that combines bromelain with trypsin and rutin.
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	At the optimum temperature, the enzyme acts the fastest, but (at least the fruit variant) is destroyed within few minutes. After an hour at 50 °C (122 °F), 83% of the enzyme remains, while at 40 °C (104 °F), practically 100% remains. As a result of this, the optimum temperature for maximum cumulated activity over time is 35-45 °C. At room temperature, the enzyme can survive at least a week even under multiple freeze-thaw cycles.		At the optimum temperature, the enzyme acts the fastest, but (at least the fruit variant) is destroyed within few minutes. After an hour at 50 °C (122 °F), 83% of the enzyme remains, while at 40 °C (104 °F), practically 100% remains. As a result of this, the optimum temperature for maximum cumulated activity over time is 35-45 °C. At room temperature, the enzyme can survive at least a week even under multiple freeze-thaw cycles.

		+	== 3D Structures of lactoferrin ==

		+	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
		+
		+	[[1w0q]] -
	== References ==		== References ==
	<references/>		<references/>

Alexander Berchansky at 14:11, 28 January 2016

Alexander Berchansky — Thu, 28 Jan 2016 14:11:56 GMT

←Older revision		Revision as of 14:11, 28 January 2016
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-
	<StructureSection load='1w0q' size='350' side='right' scene='' caption='Pineapple bromelain (PDB code [[1w0q]])'>		<StructureSection load='1w0q' size='350' side='right' scene='' caption='Pineapple bromelain (PDB code [[1w0q]])'>
	Bromelain is a protein extract derived from the stems of pineapples.The extract has a history of folk medicine use. As a culinary ingredient, it may be used as a meat tenderizer.		Bromelain is a protein extract derived from the stems of pineapples.The extract has a history of folk medicine use. As a culinary ingredient, it may be used as a meat tenderizer.

Michal Harel at 11:58, 28 January 2016

Michal Harel — Thu, 28 Jan 2016 11:58:21 GMT

←Older revision		Revision as of 11:58, 28 January 2016
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-

	<StructureSection load='1w0q' size='350' side='right' scene='' caption='Pineapple bromelain (PDB code [[1w0q]])'>		<StructureSection load='1w0q' size='350' side='right' scene='' caption='Pineapple bromelain (PDB code [[1w0q]])'>

Michal Harel at 11:57, 28 January 2016

Michal Harel — Thu, 28 Jan 2016 11:57:23 GMT

←Older revision		Revision as of 11:57, 28 January 2016
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-	<StructureSection load='1w0q' size='~~340~~' side='right' caption='Pineapple bromelain (PDB code [[1w0q]])~~' scene='~~'>	+
		+
		+	<StructureSection load='1w0q' size='350' side='right' scene='' caption='Pineapple bromelain (PDB code [[1w0q]])'>
	Bromelain is a protein extract derived from the stems of pineapples.The extract has a history of folk medicine use. As a culinary ingredient, it may be used as a meat tenderizer.		Bromelain is a protein extract derived from the stems of pineapples.The extract has a history of folk medicine use. As a culinary ingredient, it may be used as a meat tenderizer.
	Bromelain may refer to either of two protease enzymes extracted from the plants of the family Bromeliaceae, or it may refer to a combination of those enzymes along with other compounds produced in an extract.		Bromelain may refer to either of two protease enzymes extracted from the plants of the family Bromeliaceae, or it may refer to a combination of those enzymes along with other compounds produced in an extract.

Michal Harel at 11:54, 28 January 2016

Michal Harel — Thu, 28 Jan 2016 11:54:50 GMT

←Older revision		Revision as of 11:54, 28 January 2016
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-	<StructureSection load='1w0q' size='340' side='right' caption='~~Caption for this structure~~' scene=''>	+	<StructureSection load='1w0q' size='340' side='right' caption='Pineapple bromelain (PDB code [[1w0q]])' scene=''>
	Bromelain is a protein extract derived from the stems of pineapples.The extract has a history of folk medicine use. As a culinary ingredient, it may be used as a meat tenderizer.		Bromelain is a protein extract derived from the stems of pineapples.The extract has a history of folk medicine use. As a culinary ingredient, it may be used as a meat tenderizer.
	Bromelain may refer to either of two protease enzymes extracted from the plants of the family Bromeliaceae, or it may refer to a combination of those enzymes along with other compounds produced in an extract.		Bromelain may refer to either of two protease enzymes extracted from the plants of the family Bromeliaceae, or it may refer to a combination of those enzymes along with other compounds produced in an extract.

Zicheng Ye at 19:23, 22 January 2015

Zicheng Ye — Thu, 22 Jan 2015 19:23:27 GMT

←Older revision		Revision as of 19:23, 22 January 2015
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	==Temperature stability==		==Temperature stability==
	At the optimum temperature, the enzyme acts the fastest, but (at least the fruit variant) is destroyed within few minutes. After an hour at 50 °C (122 °F), 83% of the enzyme remains, while at 40 °C (104 °F), practically 100% remains. As a result of this, the optimum temperature for maximum cumulated activity over time is 35-45 °C. At room temperature, the enzyme can survive at least a week even under multiple freeze-thaw cycles.		At the optimum temperature, the enzyme acts the fastest, but (at least the fruit variant) is destroyed within few minutes. After an hour at 50 °C (122 °F), 83% of the enzyme remains, while at 40 °C (104 °F), practically 100% remains. As a result of this, the optimum temperature for maximum cumulated activity over time is 35-45 °C. At room temperature, the enzyme can survive at least a week even under multiple freeze-thaw cycles.
-
-	==3D Bromelain Structure==
-	We can also add <scene name='60/609840/Bromelain/1'>Bromelain</scene> on the 3D model.


	== References ==		== References ==
	<references/>		<references/>

Zicheng Ye at 19:21, 22 January 2015

Zicheng Ye — Thu, 22 Jan 2015 19:21:23 GMT

←Older revision		Revision as of 19:21, 22 January 2015
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	== References ==		== References ==
	<references/>		<references/>
-
-	__TOC__
-	</StructureSection>

Zicheng Ye at 12:59, 23 November 2014

Zicheng Ye — Sun, 23 Nov 2014 12:59:20 GMT

←Older revision		Revision as of 12:59, 23 November 2014
Line 1:		Line 1:
-	<StructureSection load='~~1stp~~' size='340' side='right' caption='Caption for this structure' scene=''>	+	<StructureSection load='1w0q' size='340' side='right' caption='Caption for this structure' scene=''>
	Bromelain is a protein extract derived from the stems of pineapples.The extract has a history of folk medicine use. As a culinary ingredient, it may be used as a meat tenderizer.		Bromelain is a protein extract derived from the stems of pineapples.The extract has a history of folk medicine use. As a culinary ingredient, it may be used as a meat tenderizer.
	Bromelain may refer to either of two protease enzymes extracted from the plants of the family Bromeliaceae, or it may refer to a combination of those enzymes along with other compounds produced in an extract.		Bromelain may refer to either of two protease enzymes extracted from the plants of the family Bromeliaceae, or it may refer to a combination of those enzymes along with other compounds produced in an extract.