Calcium-dependent protein kinase - Revision history

Michal Harel at 08:57, 9 November 2022

Michal Harel — Wed, 09 Nov 2022 08:57:47 GMT

←Older revision		Revision as of 08:57, 9 November 2022
Line 1:		Line 1:
	== Calcium-dependent protein kinase (CDPK)==		== Calcium-dependent protein kinase (CDPK)==
-	'''Calcium-dependent protein kinases''' (CDPKs) of '''CamK''' are found in plants, green algae, and protists. In plants CDPKs are encoded by large gene families<ref> PMID:12805596</ref><ref>PMID:15695435</ref>, and they are involved in cellular responses to many stimuli such as hormones and environmental stress<ref>PMID: 24014579</ref>. In the apicomplexan protists ''Plasmodium falciparum'' (parasite that causes malaria) and ''Toxoplasma gondii'' (parasite that causes toxoplasmosis), CDPKs are encoded by small gene families, and they are involved in critical stages of the parasite life cycle <ref>PMID:23226109</ref><ref>PMID:20466936</ref>, and they are targets for the development of drugs to fight parasitic infections <ref>PMID:20436472</ref>.	+	'''Calcium-dependent protein kinases''' (CDPKs) of '''CamK''' or '''calmodulin-domain protein kinase''' are found in plants, green algae, and protists. In plants CDPKs are encoded by large gene families<ref> PMID:12805596</ref><ref>PMID:15695435</ref>, and they are involved in cellular responses to many stimuli such as hormones and environmental stress<ref>PMID: 24014579</ref>. In the apicomplexan protists ''Plasmodium falciparum'' (parasite that causes malaria) and ''Toxoplasma gondii'' (parasite that causes toxoplasmosis), CDPKs are encoded by small gene families, and they are involved in critical stages of the parasite life cycle <ref>PMID:23226109</ref><ref>PMID:20466936</ref>, and they are targets for the development of drugs to fight parasitic infections <ref>PMID:20436472</ref>.

	For the full mammalian '''CamK''' family see [[Ca2+/Calmodulin dependent protein kinase]].		For the full mammalian '''CamK''' family see [[Ca2+/Calmodulin dependent protein kinase]].

Michal Harel at 08:51, 19 May 2021

Michal Harel — Wed, 19 May 2021 08:51:35 GMT

←Older revision		Revision as of 08:51, 19 May 2021
Line 21:		Line 21:
	{{#tree:id=OrganizedByTopic\|openlevels=0\|		{{#tree:id=OrganizedByTopic\|openlevels=0\|

-	*CDPK	+	*CDPK or calmodulin-domain protein kinase

	**[[3nyv]] – TgCDPK+WHI-P180 – ''Toxoplasma gondii''<br />		**[[3nyv]] – TgCDPK+WHI-P180 – ''Toxoplasma gondii''<br />
Line 36:		Line 36:
	**[[4rgj]] – PfCDPK4 <br />		**[[4rgj]] – PfCDPK4 <br />

-	*CDPK N-~~terminal~~	+	*CDPK calcium regulatory domain 329-402

-	**[[1s6j]] – sCDPK α chain A N-terminal – soybean<br />	+	**[[1s6i]], [[1s6j]] – sCDPK α chain A N-terminal – soybean<br />

-	*CDPK CBD	+	*CDPK CBD 30-507

		+	**[[3ku2]] - TgCDPK CBD<br />
	**[[3n51]] - TgCDPK CBD +RM-1-95<br />		**[[3n51]] - TgCDPK CBD +RM-1-95<br />
	**[[3i7b]] - TgCDPK CBD+ NM-PP1<br />		**[[3i7b]] - TgCDPK CBD+ NM-PP1<br />
	**[[3i7c]] - TgCDPK CBD+ NA-PP2<br />		**[[3i7c]] - TgCDPK CBD+ NA-PP2<br />
-	**[[~~3ku2~~]] - TgCDPK CBD<br />	+	**[[5w80]], [[5w8r]], [[5w91]], [[5w9e]], [[5w9r]], [[6bfa]] - TgCDPK CBD + inhibitor<br />
	**[[3mwu]] – CpCDPK CBD (mutant)+RM-1-95 – ''Cryptosporidium parvum''<br />		**[[3mwu]] – CpCDPK CBD (mutant)+RM-1-95 – ''Cryptosporidium parvum''<br />
	**[[3ncg]] - CpCDPK CBD + NM-PP1<br />		**[[3ncg]] - CpCDPK CBD + NM-PP1<br />
Line 52:		Line 53:
	**[[3o4y]] – CDPK3 CBD – ''Plasmodium vivax''<br />		**[[3o4y]] – CDPK3 CBD – ''Plasmodium vivax''<br />

-	*CDPK kinase domain	+	*CDPK kinase domain 72-340

	**[[3dxn]] – TgCDPK kinase domain<br />		**[[3dxn]] – TgCDPK kinase domain<br />
Line 59:		Line 60:
	**[[3dfa]] - CpCDPK kinase domain<br />		**[[3dfa]] - CpCDPK kinase domain<br />

-	*CDPK C-terminal	+	*CDPK C-terminal 388-560

	**[[1s6i]] - sCDPK α chain A C-terminal+CaM-like domain<br />		**[[1s6i]] - sCDPK α chain A C-terminal+CaM-like domain<br />

Michal Harel at 07:42, 22 March 2017

Michal Harel — Wed, 22 Mar 2017 07:42:08 GMT

←Older revision		Revision as of 07:42, 22 March 2017
Line 2:		Line 2:
	'''Calcium-dependent protein kinases''' (CDPKs) of '''CamK''' are found in plants, green algae, and protists. In plants CDPKs are encoded by large gene families<ref> PMID:12805596</ref><ref>PMID:15695435</ref>, and they are involved in cellular responses to many stimuli such as hormones and environmental stress<ref>PMID: 24014579</ref>. In the apicomplexan protists ''Plasmodium falciparum'' (parasite that causes malaria) and ''Toxoplasma gondii'' (parasite that causes toxoplasmosis), CDPKs are encoded by small gene families, and they are involved in critical stages of the parasite life cycle <ref>PMID:23226109</ref><ref>PMID:20466936</ref>, and they are targets for the development of drugs to fight parasitic infections <ref>PMID:20436472</ref>.		'''Calcium-dependent protein kinases''' (CDPKs) of '''CamK''' are found in plants, green algae, and protists. In plants CDPKs are encoded by large gene families<ref> PMID:12805596</ref><ref>PMID:15695435</ref>, and they are involved in cellular responses to many stimuli such as hormones and environmental stress<ref>PMID: 24014579</ref>. In the apicomplexan protists ''Plasmodium falciparum'' (parasite that causes malaria) and ''Toxoplasma gondii'' (parasite that causes toxoplasmosis), CDPKs are encoded by small gene families, and they are involved in critical stages of the parasite life cycle <ref>PMID:23226109</ref><ref>PMID:20466936</ref>, and they are targets for the development of drugs to fight parasitic infections <ref>PMID:20436472</ref>.

-	For the full '''CamK''' family see [[Ca2+/Calmodulin dependent protein kinase]].	+	For the full mammalian '''CamK''' family see [[Ca2+/Calmodulin dependent protein kinase]].

	CDPKs are monomeric enzymes containing an amino-terminal [[Eukaryotic Protein Kinase Catalytic Domain]] linked to a carboxy-terminal calcium-binding regulatory domain that contains four [[EF hand]] calcium-binding sites. The protein kinase domain is similar in sequence to members of the calmodulin-dependent protein kinase family<ref>PMID:1852075</ref>, and the calcium-binding domain has sequence similarity to [[calmodulin]]. CDPKs are regulated by the binding of Ca<sup>2+</sup> to the regulatory domain (called the calcium activation domain or CAD), and they are activated by processes that elevate the concentration of calcium inside cells. CDPK contains 5 domains: N-terminal, kinase, autoinhibitory junction domain, calcium-binding domain (CBD), C-terminal domain.		CDPKs are monomeric enzymes containing an amino-terminal [[Eukaryotic Protein Kinase Catalytic Domain]] linked to a carboxy-terminal calcium-binding regulatory domain that contains four [[EF hand]] calcium-binding sites. The protein kinase domain is similar in sequence to members of the calmodulin-dependent protein kinase family<ref>PMID:1852075</ref>, and the calcium-binding domain has sequence similarity to [[calmodulin]]. CDPKs are regulated by the binding of Ca<sup>2+</sup> to the regulatory domain (called the calcium activation domain or CAD), and they are activated by processes that elevate the concentration of calcium inside cells. CDPK contains 5 domains: N-terminal, kinase, autoinhibitory junction domain, calcium-binding domain (CBD), C-terminal domain.
Line 18:		Line 18:
	== 3D Structures of calcium-dependent protein kinase (CDPK) ==		== 3D Structures of calcium-dependent protein kinase (CDPK) ==

-	~~See~~ [[~~Ca2~~+/~~Calmodulin dependent protein~~ kinase]]	+	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
		+	{{#tree:id=OrganizedByTopic\|openlevels=0\|
		+
		+	*CDPK
		+
		+	**[[3nyv]] – TgCDPK+WHI-P180 – ''Toxoplasma gondii''<br />
		+	**[[3khe]], [[3i79]], [[3hzt]] – TgCaMK<br />
		+	**[[3hx4]] – TgCDPK+Ca<br />
		+	**[[3l19]], [[3igo]], [[3hko]] – CpCDPK<br />
		+	**[[2qg5]] – CpCDPK chains A, B, D<br />
		+	**[[3lij]] – CpCDPK+Ca<br />
		+	**[[2aao]] – CeCDPK chains A, B – ''Arabidopsis thaliana''<br />
		+	**[[3k21]] – PfCDPK2 residues 425-602 + Ca – ''Plasmodium falciparum''<br />
		+	**[[3pm8]] – PfCDPK2 residues 335-513 + Ca <br />
		+	**[[4mvf]] – PfCDPK2 + staurosporin + Ca <br />
		+	**[[4qox]] – PfCDPK4 + Mg <br />
		+	**[[4rgj]] – PfCDPK4 <br />
		+
		+	*CDPK N-terminal
		+
		+	**[[1s6j]] – sCDPK α chain A N-terminal – soybean<br />
		+
		+	*CDPK CBD
		+
		+	**[[3n51]] - TgCDPK CBD +RM-1-95<br />
		+	**[[3i7b]] - TgCDPK CBD+ NM-PP1<br />
		+	**[[3i7c]] - TgCDPK CBD+ NA-PP2<br />
		+	**[[3ku2]] - TgCDPK CBD<br />
		+	**[[3mwu]] – CpCDPK CBD (mutant)+RM-1-95 – ''Cryptosporidium parvum''<br />
		+	**[[3ncg]] - CpCDPK CBD + NM-PP1<br />
		+	**[[1iq5]] – CeCDPK CBD+XlCaM – ''Xenopus laevis''<br />
		+	**[[4jwq]] – CDPK3 CBD + Ca – ''Plasmodium berghei''<br />
		+	**[[3o4y]] – CDPK3 CBD – ''Plasmodium vivax''<br />
		+
		+	*CDPK kinase domain
		+
		+	**[[3dxn]] – TgCDPK kinase domain<br />
		+	**[[3is5]] – TgCDPK kinase domain + ANP + Ca<br />
		+	**[[3f3z]] – CpCDPK kinase domain+indirubin_E804<br />
		+	**[[3dfa]] - CpCDPK kinase domain<br />
		+
		+	*CDPK C-terminal
		+
		+	**[[1s6i]] - sCDPK α chain A C-terminal+CaM-like domain<br />
		+	**[[3k21]] – PfCDPK3 C terminal domain + Ca <br />
		+
		+
		+	}}
	=References=		=References=

Michal Harel at 10:07, 16 November 2015

Michal Harel — Mon, 16 Nov 2015 10:07:08 GMT

←Older revision		Revision as of 10:07, 16 November 2015
Line 1:		Line 1:
	== Calcium-dependent protein kinase (CDPK)==		== Calcium-dependent protein kinase (CDPK)==
	'''Calcium-dependent protein kinases''' (CDPKs) of '''CamK''' are found in plants, green algae, and protists. In plants CDPKs are encoded by large gene families<ref> PMID:12805596</ref><ref>PMID:15695435</ref>, and they are involved in cellular responses to many stimuli such as hormones and environmental stress<ref>PMID: 24014579</ref>. In the apicomplexan protists ''Plasmodium falciparum'' (parasite that causes malaria) and ''Toxoplasma gondii'' (parasite that causes toxoplasmosis), CDPKs are encoded by small gene families, and they are involved in critical stages of the parasite life cycle <ref>PMID:23226109</ref><ref>PMID:20466936</ref>, and they are targets for the development of drugs to fight parasitic infections <ref>PMID:20436472</ref>.		'''Calcium-dependent protein kinases''' (CDPKs) of '''CamK''' are found in plants, green algae, and protists. In plants CDPKs are encoded by large gene families<ref> PMID:12805596</ref><ref>PMID:15695435</ref>, and they are involved in cellular responses to many stimuli such as hormones and environmental stress<ref>PMID: 24014579</ref>. In the apicomplexan protists ''Plasmodium falciparum'' (parasite that causes malaria) and ''Toxoplasma gondii'' (parasite that causes toxoplasmosis), CDPKs are encoded by small gene families, and they are involved in critical stages of the parasite life cycle <ref>PMID:23226109</ref><ref>PMID:20466936</ref>, and they are targets for the development of drugs to fight parasitic infections <ref>PMID:20436472</ref>.
		+
		+	For the full '''CamK''' family see [[Ca2+/Calmodulin dependent protein kinase]].

	CDPKs are monomeric enzymes containing an amino-terminal [[Eukaryotic Protein Kinase Catalytic Domain]] linked to a carboxy-terminal calcium-binding regulatory domain that contains four [[EF hand]] calcium-binding sites. The protein kinase domain is similar in sequence to members of the calmodulin-dependent protein kinase family<ref>PMID:1852075</ref>, and the calcium-binding domain has sequence similarity to [[calmodulin]]. CDPKs are regulated by the binding of Ca<sup>2+</sup> to the regulatory domain (called the calcium activation domain or CAD), and they are activated by processes that elevate the concentration of calcium inside cells. CDPK contains 5 domains: N-terminal, kinase, autoinhibitory junction domain, calcium-binding domain (CBD), C-terminal domain.		CDPKs are monomeric enzymes containing an amino-terminal [[Eukaryotic Protein Kinase Catalytic Domain]] linked to a carboxy-terminal calcium-binding regulatory domain that contains four [[EF hand]] calcium-binding sites. The protein kinase domain is similar in sequence to members of the calmodulin-dependent protein kinase family<ref>PMID:1852075</ref>, and the calcium-binding domain has sequence similarity to [[calmodulin]]. CDPKs are regulated by the binding of Ca<sup>2+</sup> to the regulatory domain (called the calcium activation domain or CAD), and they are activated by processes that elevate the concentration of calcium inside cells. CDPK contains 5 domains: N-terminal, kinase, autoinhibitory junction domain, calcium-binding domain (CBD), C-terminal domain.

Michal Harel at 11:41, 15 November 2015

Michal Harel — Sun, 15 Nov 2015 11:41:02 GMT

←Older revision		Revision as of 11:41, 15 November 2015
Line 16:		Line 16:
	== 3D Structures of calcium-dependent protein kinase (CDPK) ==		== 3D Structures of calcium-dependent protein kinase (CDPK) ==

-	See [[Ca+2/Calmodulin dependent protein kinase]]	+	See [[Ca2+/Calmodulin dependent protein kinase]]
	=References=		=References=

Michal Harel at 11:38, 15 November 2015

Michal Harel — Sun, 15 Nov 2015 11:38:30 GMT

←Older revision		Revision as of 11:38, 15 November 2015
Line 1:		Line 1:
	== Calcium-dependent protein kinase (CDPK)==		== Calcium-dependent protein kinase (CDPK)==
-	Calcium-dependent protein kinases (CDPKs) are found in plants, green algae, and protists. In plants CDPKs are encoded by large gene families<ref> PMID:12805596</ref><ref>PMID:15695435</ref>, and they are involved in cellular responses to many stimuli such as hormones and environmental stress<ref>PMID: 24014579</ref>. In the apicomplexan protists ''Plasmodium falciparum'' (parasite that causes malaria) and ''Toxoplasma gondii'' (parasite that causes toxoplasmosis), CDPKs are encoded by small gene families, and they are involved in critical stages of the parasite life cycle <ref>PMID:23226109</ref><ref>PMID:20466936</ref>, and they are targets for the development of drugs to fight parasitic infections <ref>PMID:20436472</ref>.	+	'''Calcium-dependent protein kinases''' (CDPKs) of '''CamK''' are found in plants, green algae, and protists. In plants CDPKs are encoded by large gene families<ref> PMID:12805596</ref><ref>PMID:15695435</ref>, and they are involved in cellular responses to many stimuli such as hormones and environmental stress<ref>PMID: 24014579</ref>. In the apicomplexan protists ''Plasmodium falciparum'' (parasite that causes malaria) and ''Toxoplasma gondii'' (parasite that causes toxoplasmosis), CDPKs are encoded by small gene families, and they are involved in critical stages of the parasite life cycle <ref>PMID:23226109</ref><ref>PMID:20466936</ref>, and they are targets for the development of drugs to fight parasitic infections <ref>PMID:20436472</ref>.

	CDPKs are monomeric enzymes containing an amino-terminal [[Eukaryotic Protein Kinase Catalytic Domain]] linked to a carboxy-terminal calcium-binding regulatory domain that contains four [[EF hand]] calcium-binding sites. The protein kinase domain is similar in sequence to members of the calmodulin-dependent protein kinase family<ref>PMID:1852075</ref>, and the calcium-binding domain has sequence similarity to [[calmodulin]]. CDPKs are regulated by the binding of Ca<sup>2+</sup> to the regulatory domain (called the calcium activation domain or CAD), and they are activated by processes that elevate the concentration of calcium inside cells. CDPK contains 5 domains: N-terminal, kinase, autoinhibitory junction domain, calcium-binding domain (CBD), C-terminal domain.		CDPKs are monomeric enzymes containing an amino-terminal [[Eukaryotic Protein Kinase Catalytic Domain]] linked to a carboxy-terminal calcium-binding regulatory domain that contains four [[EF hand]] calcium-binding sites. The protein kinase domain is similar in sequence to members of the calmodulin-dependent protein kinase family<ref>PMID:1852075</ref>, and the calcium-binding domain has sequence similarity to [[calmodulin]]. CDPKs are regulated by the binding of Ca<sup>2+</sup> to the regulatory domain (called the calcium activation domain or CAD), and they are activated by processes that elevate the concentration of calcium inside cells. CDPK contains 5 domains: N-terminal, kinase, autoinhibitory junction domain, calcium-binding domain (CBD), C-terminal domain.
Line 16:		Line 16:
	== 3D Structures of calcium-dependent protein kinase (CDPK) ==		== 3D Structures of calcium-dependent protein kinase (CDPK) ==

-	~~Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}~~	+	See [[Ca+2/Calmodulin dependent protein kinase]]
-	~~{{#tree:id=OrganizedByTopic\|openlevels=0\|~~	+
-		+
-	*CDPK	+
-		+
-	**[[~~3nyv]] – TgCDPK+WHI-P180 – ''Toxoplasma gondii''<br />~~	+
-	**[[3khe]], [[3i79]], [[3hzt]] – TgCaMK<br />	+
-	**[[3hx4]] – TgCDPK+Ca~~<br />~~	+
-	**[[3l19]], [[3igo]], [[3hko]] – CpCDPK<br />	+
-	**[[2qg5]] – CpCDPK chains A, B, D<br />	+
-	**[[3lij]] – CpCDPK+~~Ca<br~~ />	+
-	**[[2aao]] – CeCDPK chains A, B – ''Arabidopsis thaliana''<br />	+
-	**[[3k21]] – PfCDPK2 residues 425-602 + Ca – ''Plasmodium falciparum''<br />	+
-	**[[3pm8]] – PfCDPK2 residues 335-513 + Ca <br />	+
-	**[[4mvf]] – PfCDPK2 + staurosporin + Ca <br />	+
-	**[[4qox]] – PfCDPK4 + Mg <br />	+
-	**[[4rgj]] – PfCDPK4 <br />	+
-		+
-	*CDPK N-terminal	+
-		+
-	**[[1s6j]] – sCDPK α chain A N-terminal – soybean<br />	+
-		+
-	*CDPK CBD	+
-		+
-	**[[3n51]] - TgCDPK CBD +RM-1-95<br />	+
-	**[[3i7b]] - TgCDPK CBD+ NM-PP1<br />	+
-	**[[3i7c]] - TgCDPK CBD+ NA-PP2<br />	+
-	**[[3ku2]] - TgCDPK CBD<br />	+
-	**[[3mwu]] – CpCDPK CBD (mutant)+RM-1-95 – ''Cryptosporidium parvum''<br />	+
-	**[[3ncg]] - CpCDPK CBD + NM-PP1<br />	+
-	**[[1iq5]] – CeCDPK CBD+XlCaM – ''Xenopus laevis''<br />	+
-	**[[4jwq]] – CDPK3 CBD + Ca – ''Plasmodium berghei''<br />	+
-	**[[3o4y]] – CDPK3 CBD – ''Plasmodium vivax''<br />	+
-		+
-	*CDPK kinase ~~domain~~	+
-		+
-	**[[3dxn]] ~~– TgCDPK kinase domain<br />~~	+
-	**[[3is5]] – TgCDPK kinase domain + ANP + Ca<br />	+
-	**[[3f3z]] – CpCDPK kinase domain+indirubin_E804<br />	+
-	**[[3dfa]] - CpCDPK kinase domain<br />	+
-		+
-	*CDPK C-terminal	+
-		+
-	**[[1s6i]] - sCDPK α chain A C-terminal+CaM-like domain<br />	+
-	**[[3k21]] – PfCDPK3 C terminal domain + Ca <br />	+
-	}}	+
	=References=		=References=

Michal Harel at 09:37, 9 March 2015

Michal Harel — Mon, 09 Mar 2015 09:37:11 GMT

←Older revision		Revision as of 09:37, 9 March 2015
Line 29:		Line 29:
	**[[2aao]] – CeCDPK chains A, B – ''Arabidopsis thaliana''<br />		**[[2aao]] – CeCDPK chains A, B – ''Arabidopsis thaliana''<br />
	**[[3k21]] – PfCDPK2 residues 425-602 + Ca – ''Plasmodium falciparum''<br />		**[[3k21]] – PfCDPK2 residues 425-602 + Ca – ''Plasmodium falciparum''<br />
		+	**[[3pm8]] – PfCDPK2 residues 335-513 + Ca <br />
		+	**[[4mvf]] – PfCDPK2 + staurosporin + Ca <br />
		+	**[[4qox]] – PfCDPK4 + Mg <br />
		+	**[[4rgj]] – PfCDPK4 <br />

	*CDPK N-terminal		*CDPK N-terminal

Michal Harel at 11:20, 19 November 2014

Michal Harel — Wed, 19 Nov 2014 11:20:45 GMT

←Older revision		Revision as of 11:20, 19 November 2014
Line 17:		Line 17:

	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}		Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
		+	{{#tree:id=OrganizedByTopic\|openlevels=0\|

-	~~===~~CDPK~~===~~	+	*CDPK

-	[[3nyv]] – TgCDPK+WHI-P180 – ''Toxoplasma gondii''<br />	+	**[[3nyv]] – TgCDPK+WHI-P180 – ''Toxoplasma gondii''<br />
-	[[3khe]], [[3i79]], [[3hzt]] – TgCaMK<br />	+	**[[3khe]], [[3i79]], [[3hzt]] – TgCaMK<br />
-	[[3hx4]] – TgCDPK+Ca<br />	+	**[[3hx4]] – TgCDPK+Ca<br />
-	[[3l19]], [[3igo]], [[3hko]] – CpCDPK<br />	+	**[[3l19]], [[3igo]], [[3hko]] – CpCDPK<br />
-	[[2qg5]] – CpCDPK chains A, B, D<br />	+	**[[2qg5]] – CpCDPK chains A, B, D<br />
-	[[3lij]] – CpCDPK+Ca<br />	+	**[[3lij]] – CpCDPK+Ca<br />
-	[[2aao]] – CeCDPK chains A, B – ''Arabidopsis thaliana''<br />	+	**[[2aao]] – CeCDPK chains A, B – ''Arabidopsis thaliana''<br />
-	[[3k21]] – PfCDPK2 residues 425-602 + Ca – ''Plasmodium falciparum''<br />	+	**[[3k21]] – PfCDPK2 residues 425-602 + Ca – ''Plasmodium falciparum''<br />

-	~~===~~CDPK N-terminal~~===~~	+	*CDPK N-terminal

-	[[1s6j]] – sCDPK α chain A N-terminal – soybean<br />	+	**[[1s6j]] – sCDPK α chain A N-terminal – soybean<br />

-	~~===~~CDPK CBD~~===~~	+	*CDPK CBD

-	[[3n51]] - TgCDPK CBD +RM-1-95<br />	+	**[[3n51]] - TgCDPK CBD +RM-1-95<br />
-	[[3i7b]] - TgCDPK CBD+ NM-PP1<br />	+	**[[3i7b]] - TgCDPK CBD+ NM-PP1<br />
-	[[3i7c]] - TgCDPK CBD+ NA-PP2<br />	+	**[[3i7c]] - TgCDPK CBD+ NA-PP2<br />
-	[[3ku2]] - TgCDPK CBD<br />	+	**[[3ku2]] - TgCDPK CBD<br />
-	[[3mwu]] – CpCDPK CBD (mutant)+RM-1-95 – ''Cryptosporidium parvum''<br />	+	**[[3mwu]] – CpCDPK CBD (mutant)+RM-1-95 – ''Cryptosporidium parvum''<br />
-	[[3ncg]] - CpCDPK CBD + NM-PP1<br />	+	**[[3ncg]] - CpCDPK CBD + NM-PP1<br />
-	[[1iq5]] – CeCDPK CBD+XlCaM – ''Xenopus laevis''<br />	+	**[[1iq5]] – CeCDPK CBD+XlCaM – ''Xenopus laevis''<br />
-	[[4jwq]] – CDPK3 CBD + Ca – ''Plasmodium berghei''<br />	+	**[[4jwq]] – CDPK3 CBD + Ca – ''Plasmodium berghei''<br />
-	[[3o4y]] – CDPK3 CBD – ''Plasmodium vivax''<br />	+	**[[3o4y]] – CDPK3 CBD – ''Plasmodium vivax''<br />

-	~~===~~CDPK kinase domain~~===~~	+	*CDPK kinase domain

-	[[3dxn]] – TgCDPK kinase domain<br />	+	**[[3dxn]] – TgCDPK kinase domain<br />
-	[[3is5]] – TgCDPK kinase domain + ANP + Ca<br />	+	**[[3is5]] – TgCDPK kinase domain + ANP + Ca<br />
-	[[3f3z]] – CpCDPK kinase domain+indirubin_E804<br />	+	**[[3f3z]] – CpCDPK kinase domain+indirubin_E804<br />
-	[[3dfa]] - CpCDPK kinase domain<br />	+	**[[3dfa]] - CpCDPK kinase domain<br />

-	~~===~~CDPK C-terminal~~===~~	+	*CDPK C-terminal
-		+
-	~~[[1s6i]] - sCDPK α chain A C-terminal+CaM-like domain<br />~~	+
-	~~[[3k21]] – PfCDPK3 C terminal domain + Ca <br />~~	+

		+	**[[1s6i]] - sCDPK α chain A C-terminal+CaM-like domain<br />
		+	**[[3k21]] – PfCDPK3 C terminal domain + Ca <br />
		+	}}
	=References=		=References=

Michal Harel at 06:40, 17 August 2014

Michal Harel — Sun, 17 Aug 2014 06:40:01 GMT

←Older revision		Revision as of 06:40, 17 August 2014
Line 17:		Line 17:

	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}		Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
		+
		+	===CDPK===

	[[3nyv]] – TgCDPK+WHI-P180 – ''Toxoplasma gondii''<br />		[[3nyv]] – TgCDPK+WHI-P180 – ''Toxoplasma gondii''<br />
Line 27:		Line 29:
	[[3k21]] – PfCDPK2 residues 425-602 + Ca – ''Plasmodium falciparum''<br />		[[3k21]] – PfCDPK2 residues 425-602 + Ca – ''Plasmodium falciparum''<br />

-	~~'''~~CDPK N-terminal~~'''~~	+	===CDPK N-terminal===

	[[1s6j]] – sCDPK α chain A N-terminal – soybean<br />		[[1s6j]] – sCDPK α chain A N-terminal – soybean<br />

-	~~'''~~CDPK CBD~~'''~~	+	===CDPK CBD===

	[[3n51]] - TgCDPK CBD +RM-1-95<br />		[[3n51]] - TgCDPK CBD +RM-1-95<br />
Line 43:		Line 45:
	[[3o4y]] – CDPK3 CBD – ''Plasmodium vivax''<br />		[[3o4y]] – CDPK3 CBD – ''Plasmodium vivax''<br />

-	~~'''~~CDPK kinase domain~~'''~~	+	===CDPK kinase domain===

	[[3dxn]] – TgCDPK kinase domain<br />		[[3dxn]] – TgCDPK kinase domain<br />
Line 50:		Line 52:
	[[3dfa]] - CpCDPK kinase domain<br />		[[3dfa]] - CpCDPK kinase domain<br />

-	~~'''~~CDPK C-terminal~~'''~~	+	===CDPK C-terminal===

	[[1s6i]] - sCDPK α chain A C-terminal+CaM-like domain<br />		[[1s6i]] - sCDPK α chain A C-terminal+CaM-like domain<br />

Alice Harmon at 15:07, 26 July 2014

Alice Harmon — Sat, 26 Jul 2014 15:07:55 GMT

←Older revision		Revision as of 15:07, 26 July 2014
Line 10:		Line 10:
	\|'''Right scene''' - The crystal structure [[3hx4]] shows the '''active conformation''' of the kinase that is bound to four calcium ions (green spheres), each bound to an [[EF hand]]. As in the left scene the catalytic domain is blue and the CAD is gold, and the ATP analog ANP is shown in wireframe and CPK coloring. In these default still scenes the large lobe of the kinase domain is in approximately the same orientation. Calcium-bound CAD interacts with the side of the kinase domain that is opposite from the catalytic cleft, making it available for peptide substrate binding.		\|'''Right scene''' - The crystal structure [[3hx4]] shows the '''active conformation''' of the kinase that is bound to four calcium ions (green spheres), each bound to an [[EF hand]]. As in the left scene the catalytic domain is blue and the CAD is gold, and the ATP analog ANP is shown in wireframe and CPK coloring. In these default still scenes the large lobe of the kinase domain is in approximately the same orientation. Calcium-bound CAD interacts with the side of the kinase domain that is opposite from the catalytic cleft, making it available for peptide substrate binding.
	\|-		\|-
-	\| <applet name= 'left' load='3ku2' size='400' frame='true' align='left' caption='[[3ku2]] - inactive TgCDPK1 complex with phsphoaminophosphonic acid adenylate ester' scene = '56/562377/Holo-ikinase/1' /><br clear='both'>'''3ku2 scenes''' <Br><scene name='56/562377/Holo-ikinase/1' target='left'>1. Default scene</scene><scene name='56/562377/Holo-ikinase/1' target='left'>1. Default scene</scene><Br><scene name='56/562377/Holo-ikinase_cad_rainbow/1'target='left'>2. Apo CAD</scene> shown in rainbow colors starting with blue at the N-terminal end to red at the C-terminal end of the domain. <br><scene name='56/562377/Holo-ikinase_cad_rainbow/2'target='left'>3. Apo CAD central helices</scene> are long and straight.<br><scene name='56/562377/Kd_critical/1'target='left'>4. Inactive kinase domain critical structures</scene>: ATP binding loop in lime; Subdomain III in orchid; catalytic loop in blue; Mg2+ loop/activation loop in gold. <br><br><br>	+	\| <applet name= 'left' load='3ku2' size='400' frame='true' align='left' caption='[[3ku2]] - inactive TgCDPK1 complex with phsphoaminophosphonic acid adenylate ester' scene = '56/562377/Holo-ikinase/1' /><br clear='both'>'''3ku2 scenes''' <Br><scene name='56/562377/Holo-ikinase/1' target='left'>1. Default scene</scene><scene name='56/562377/Holo-ikinase/1' target='left'>1. Default scene</scene><Br><scene name='56/562377/Holo-ikinase_cad_rainbow/1' target='left'>2. Apo CAD</scene> shown in rainbow colors starting with blue at the N-terminal end to red at the C-terminal end of the domain. <br><scene name='56/562377/Holo-ikinase_cad_rainbow/2' target='left'>3. Apo CAD central helices</scene> are long and straight.<br><scene name='56/562377/Kd_critical/1' target='left'>4. Inactive kinase domain critical structures</scene>: ATP binding loop in lime; Subdomain III in orchid; catalytic loop in blue; Mg2+ loop/activation loop in gold. <br><br><br>
-	\| <applet name= 'right' load='3HX4' size='400' frame='true' align='left' caption='[[3hx4]] - active TgCDPK1 complex with ~~phsphoaminophosphonic~~ acid adenylate ester and Ca+2 ions' scene = '56/562377/Holo-akinase/1' /><Br clear='both'>'''3hx4 scenes'''<Br><scene name='56/562377/Holo-akinase/1' target='right'>1. Default Scene</scene><scene name='56/562377/Holo-akinase/1' target='right'>1. Default Scene</scene><Br><scene name='56/562377/Holo-akinase_cad_rainbow/2' target='right'>2. Calcium-bound CAD</scene> is more compact as a result of rearrangement of secondary structures.<br><scene name='56/562377/Holo-akinase_cad_rainbow/1' target='right'>3. Calcium-bound CAD central helices</scene> are each broken into horseshoe shapes.<br><scene name='56/562377/Akd_critical/1' target='right'>4. Active kinase domain critical structures</scene> are in the same color scheme as in the left scene, and the large lobe is in approximately the same orientation. Note the large change in the activation loop (gold). In the small lobe there is a change in shape and position of the ATP-binding loop (green) and ANP (CPK), and there is a change in position of subdomain III (orchid). These latter changes indicate twisting of the small lobe relative to the large lobe.	+	\| <applet name= 'right' load='3HX4' size='400' frame='true' align='left' caption='[[3hx4]] - active TgCDPK1 complex with phosphoaminophosphonic acid adenylate ester and Ca2+ ions' scene = '56/562377/Holo-akinase/1' /><Br clear='both'>'''3hx4 scenes'''<Br><scene name='56/562377/Holo-akinase/1' target='right'>1. Default Scene</scene><scene name='56/562377/Holo-akinase/1' target='right'>1. Default Scene</scene><Br><scene name='56/562377/Holo-akinase_cad_rainbow/2' target='right'>2. Calcium-bound CAD</scene> is more compact as a result of rearrangement of secondary structures.<br><scene name='56/562377/Holo-akinase_cad_rainbow/1' target='right'>3. Calcium-bound CAD central helices</scene> are each broken into horseshoe shapes.<br><scene name='56/562377/Akd_critical/1' target='right'>4. Active kinase domain critical structures</scene> are in the same color scheme as in the left scene, and the large lobe is in approximately the same orientation. Note the large change in the activation loop (gold). In the small lobe there is a change in shape and position of the ATP-binding loop (green) and ANP (CPK), and there is a change in position of subdomain III (orchid). These latter changes indicate twisting of the small lobe relative to the large lobe.
	\|}		\|}