Cholesterol oxidase - Revision history

Michal Harel at 10:23, 12 May 2019

Michal Harel — Sun, 12 May 2019 10:23:09 GMT

←Older revision		Revision as of 10:23, 12 May 2019
Line 21:		Line 21:

	**[[3cox]], [[1i19]] – BsCOX – ''Brevibacterium sterolicum''<br />		**[[3cox]], [[1i19]] – BsCOX – ''Brevibacterium sterolicum''<br />
-	**[[1b4v]], [[1mxt]], [[1n1p]], [[1n4u]], [[1n4v]], [[1n4w]], [[2gew]], [[4u2l]], [[4u2s]], [[4u2t]], [[5kwf]], [[4xxg]], [[4xwr]] – StCOX – ''Streptomyces''<br />	+	**[[1b4v]], [[1mxt]], [[1n1p]], [[1n4u]], [[1n4v]], [[1n4w]], [[2gew]], [[4u2l]], [[4u2s]], [[4u2t]], [[5kwf]], [[4xxg]], [[4xwr]], [[4rek]] – StCOX – ''Streptomyces''<br />
	**[[1b8s]], [[1cbo]], [[1cc2]], [[1ijh]], [[3b6d]], [[3cnj]], [[3gyi]], [[3gyj]] – StCOX (mutant)<br />		**[[1b8s]], [[1cbo]], [[1cc2]], [[1ijh]], [[3b6d]], [[3cnj]], [[3gyi]], [[3gyj]] – StCOX (mutant)<br />
	**[[3js8]] – COX – ''Chromobacterium''<br />		**[[3js8]] – COX – ''Chromobacterium''<br />

Alexander Berchansky at 10:25, 13 February 2019

Alexander Berchansky — Wed, 13 Feb 2019 10:25:54 GMT

←Older revision		Revision as of 10:25, 13 February 2019
Line 11:		Line 11:
	== Structural highlights ==		== Structural highlights ==

-	There are predominantly hydrophobic/non-polar residues in <scene name='48/486379/Cv/3'>steroid substrate binding site</scene> ({{Template:ColorKey_Hydrophobic}}, {{Template:ColorKey_Polar}}) in COX. <scene name='48/486379/Cv/6'>FAD binding site</scene> (PDB entry [[1coy]]).<ref>PMID:8218217</ref>	+	There are predominantly hydrophobic/non-polar residues in <scene name='48/486379/Cv/7'>steroid substrate binding site</scene> ({{Template:ColorKey_Hydrophobic}}, {{Template:ColorKey_Polar}}) in COX. <scene name='48/486379/Cv/8'>FAD binding site</scene> (PDB entry [[1coy]]).<ref>PMID:8218217</ref> Water molecules are shown as red spheres. <scene name='48/486379/Cv/9'>Whole active site</scene>.
	</StructureSection>		</StructureSection>
	==3D structure of cholesterol oxidase==		==3D structure of cholesterol oxidase==

Michal Harel at 06:37, 3 April 2017

Michal Harel — Mon, 03 Apr 2017 06:37:16 GMT

←Older revision		Revision as of 06:37, 3 April 2017
Line 21:		Line 21:

	**[[3cox]], [[1i19]] – BsCOX – ''Brevibacterium sterolicum''<br />		**[[3cox]], [[1i19]] – BsCOX – ''Brevibacterium sterolicum''<br />
-	**[[1b4v]], [[1mxt]], [[1n1p]], [[1n4u]], [[1n4v]], [[1n4w]], [[2gew]], [[4u2l]], [[4u2s]], [[4u2t]] – StCOX – ''Streptomyces''<br />	+	**[[1b4v]], [[1mxt]], [[1n1p]], [[1n4u]], [[1n4v]], [[1n4w]], [[2gew]], [[4u2l]], [[4u2s]], [[4u2t]], [[5kwf]], [[4xxg]], [[4xwr]] – StCOX – ''Streptomyces''<br />
	**[[1b8s]], [[1cbo]], [[1cc2]], [[1ijh]], [[3b6d]], [[3cnj]], [[3gyi]], [[3gyj]] – StCOX (mutant)<br />		**[[1b8s]], [[1cbo]], [[1cc2]], [[1ijh]], [[3b6d]], [[3cnj]], [[3gyi]], [[3gyj]] – StCOX (mutant)<br />
	**[[3js8]] – COX – ''Chromobacterium''<br />		**[[3js8]] – COX – ''Chromobacterium''<br />

Michal Harel at 11:08, 14 November 2016

Michal Harel — Mon, 14 Nov 2016 11:08:17 GMT

←Older revision		Revision as of 11:08, 14 November 2016
Line 1:		Line 1:
-	<StructureSection load='1coy' size='~~450~~' side='right' caption='Cholesterol oxidase complex with steroid substrate (PDB entry [[1coy]])' scene='48/486379/Cv/1'>	+	<StructureSection load='1coy' size='350' side='right' caption='Cholesterol oxidase complex with steroid substrate (PDB entry [[1coy]])' scene='48/486379/Cv/1'>

	== Function ==		== Function ==
Line 20:		Line 20:
	*Cholesterol oxidase		*Cholesterol oxidase

-	**[[~~1cox~~]], [[1i19]] – BsCOX – ''Brevibacterium sterolicum''<br />	+	**[[3cox]], [[1i19]] – BsCOX – ''Brevibacterium sterolicum''<br />
	**[[1b4v]], [[1mxt]], [[1n1p]], [[1n4u]], [[1n4v]], [[1n4w]], [[2gew]], [[4u2l]], [[4u2s]], [[4u2t]] – StCOX – ''Streptomyces''<br />		**[[1b4v]], [[1mxt]], [[1n1p]], [[1n4u]], [[1n4v]], [[1n4w]], [[2gew]], [[4u2l]], [[4u2s]], [[4u2t]] – StCOX – ''Streptomyces''<br />
	**[[1b8s]], [[1cbo]], [[1cc2]], [[1ijh]], [[3b6d]], [[3cnj]], [[3gyi]], [[3gyj]] – StCOX (mutant)<br />		**[[1b8s]], [[1cbo]], [[1cc2]], [[1ijh]], [[3b6d]], [[3cnj]], [[3gyi]], [[3gyj]] – StCOX (mutant)<br />

Alexander Berchansky at 09:55, 19 January 2016

Alexander Berchansky — Tue, 19 Jan 2016 09:55:45 GMT

←Older revision		Revision as of 09:55, 19 January 2016
Line 1:		Line 1:
-	<StructureSection load='1coy' size='~~350~~' side='right' caption='Cholesterol oxidase complex with steroid substrate (PDB entry [[1coy]])' scene='48/486379/Cv/1'>	+	<StructureSection load='1coy' size='450' side='right' caption='Cholesterol oxidase complex with steroid substrate (PDB entry [[1coy]])' scene='48/486379/Cv/1'>

	== Function ==		== Function ==

Alexander Berchansky at 09:53, 19 January 2016

Alexander Berchansky — Tue, 19 Jan 2016 09:53:25 GMT

←Older revision		Revision as of 09:53, 19 January 2016
Line 11:		Line 11:
	== Structural highlights ==		== Structural highlights ==

-	There are predominantly hydrophobic/non-polar residues in <scene name='48/486379/Cv/3'>steroid substrate binding site</scene> ({{Template:ColorKey_Hydrophobic}}, {{Template:ColorKey_Polar}}) in COX. <scene name='48/486379/Cv/6'>FAD binding site</scene> (PDB entry [[1coy]]).	+	There are predominantly hydrophobic/non-polar residues in <scene name='48/486379/Cv/3'>steroid substrate binding site</scene> ({{Template:ColorKey_Hydrophobic}}, {{Template:ColorKey_Polar}}) in COX. <scene name='48/486379/Cv/6'>FAD binding site</scene> (PDB entry [[1coy]]).<ref>PMID:8218217</ref>
	</StructureSection>		</StructureSection>
	==3D structure of cholesterol oxidase==		==3D structure of cholesterol oxidase==

Alexander Berchansky at 09:52, 19 January 2016

Alexander Berchansky — Tue, 19 Jan 2016 09:52:30 GMT

←Older revision		Revision as of 09:52, 19 January 2016
Line 11:		Line 11:
	== Structural highlights ==		== Structural highlights ==

-	There are predominantly hydrophobic/non-polar residues in <scene name='48/486379/Cv/3'>steroid substrate binding site</scene> ({{Template:ColorKey_Hydrophobic}}, {{Template:ColorKey_Polar}}) in COX (PDB entry [[1coy]]).	+	There are predominantly hydrophobic/non-polar residues in <scene name='48/486379/Cv/3'>steroid substrate binding site</scene> ({{Template:ColorKey_Hydrophobic}}, {{Template:ColorKey_Polar}}) in COX. <scene name='48/486379/Cv/6'>FAD binding site</scene> (PDB entry [[1coy]]).
	</StructureSection>		</StructureSection>
	==3D structure of cholesterol oxidase==		==3D structure of cholesterol oxidase==

Alexander Berchansky at 09:03, 19 January 2016

Alexander Berchansky — Tue, 19 Jan 2016 09:03:05 GMT

←Older revision		Revision as of 09:03, 19 January 2016
Line 11:		Line 11:
	== Structural highlights ==		== Structural highlights ==

-	There are predominantly hydrophobic/non-polar residues ~~({{Template:ColorKey_Hydrophobic}}, {{Template:ColorKey_Polar}})~~ in <scene name='48/486379/Cv/3'>steroid substrate binding site</scene> in COX (PDB entry [[1coy]]).	+	There are predominantly hydrophobic/non-polar residues in <scene name='48/486379/Cv/3'>steroid substrate binding site</scene> ({{Template:ColorKey_Hydrophobic}}, {{Template:ColorKey_Polar}}) in COX (PDB entry [[1coy]]).
	</StructureSection>		</StructureSection>
	==3D structure of cholesterol oxidase==		==3D structure of cholesterol oxidase==

Alexander Berchansky at 09:02, 19 January 2016

Alexander Berchansky — Tue, 19 Jan 2016 09:02:28 GMT

←Older revision		Revision as of 09:02, 19 January 2016
Line 11:		Line 11:
	== Structural highlights ==		== Structural highlights ==

-	There are predominantly non-polar residues in <scene name='48/486379/Cv/3'>steroid substrate binding site</scene> in COX (PDB entry [[1coy]]).	+	There are predominantly hydrophobic/non-polar residues ({{Template:ColorKey_Hydrophobic}}, {{Template:ColorKey_Polar}}) in <scene name='48/486379/Cv/3'>steroid substrate binding site</scene> in COX (PDB entry [[1coy]]).
	</StructureSection>		</StructureSection>
	==3D structure of cholesterol oxidase==		==3D structure of cholesterol oxidase==

Alexander Berchansky at 09:00, 19 January 2016

Alexander Berchansky — Tue, 19 Jan 2016 09:00:48 GMT

←Older revision		Revision as of 09:00, 19 January 2016
Line 7:		Line 7:
	== Relevance ==		== Relevance ==

-	COX is used as a biosensor for the detection of cholesterol in blood serum and food.	+	COX is used as a biosensor for the detection of cholesterol in blood serum and food.
		+
		+	== Structural highlights ==
		+
		+	There are predominantly non-polar residues in <scene name='48/486379/Cv/3'>steroid substrate binding site</scene> in COX (PDB entry [[1coy]]).
	</StructureSection>		</StructureSection>
	==3D structure of cholesterol oxidase==		==3D structure of cholesterol oxidase==