Colicin E7 - Revision history

Alexander Berchansky at 14:06, 18 December 2014

Alexander Berchansky — Thu, 18 Dec 2014 14:06:57 GMT

←Older revision		Revision as of 14:06, 18 December 2014
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	The endonuclease domain is likely to use Zn<sup>2+</sup><ref> PMID: 11917029 </ref> in the DNA cleavage, although other literature does not support this<ref> PMID: 15190054 </ref>. For cleavage of single stranded DNA or RNA ColE7 can either use Ni<sup>2+</sup> or Co<sup>2+</sup><ref>PMID: 12162738</ref><ref>PMID: 11733993 </ref>. The bound ion binds to the second histadine residue in the HNH motif and stabilises the reaction.		The endonuclease domain is likely to use Zn<sup>2+</sup><ref> PMID: 11917029 </ref> in the DNA cleavage, although other literature does not support this<ref> PMID: 15190054 </ref>. For cleavage of single stranded DNA or RNA ColE7 can either use Ni<sup>2+</sup> or Co<sup>2+</sup><ref>PMID: 12162738</ref><ref>PMID: 11733993 </ref>. The bound ion binds to the second histadine residue in the HNH motif and stabilises the reaction.
-		+	</StructureSection>
	==References==		==References==

	<references/>		<references/>

Alexander Berchansky at 14:06, 18 December 2014

Alexander Berchansky — Thu, 18 Dec 2014 14:06:22 GMT

←Older revision		Revision as of 14:06, 18 December 2014
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		+	<StructureSection load='2axc' size='450' side='right' scene='Colicin_E7/Cole7t/1' caption=''>
	Colicin E7 is a type of [[Colicin]], a bacteriocin made by ''E. coli'' which acts against other nearby ''E. coli'' to kill them with its [[DNase Activity]]; it digests the cell's genome in specific locations, ultimately leading to the death of the cell.		Colicin E7 is a type of [[Colicin]], a bacteriocin made by ''E. coli'' which acts against other nearby ''E. coli'' to kill them with its [[DNase Activity]]; it digests the cell's genome in specific locations, ultimately leading to the death of the cell.

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	==Mechanism of uptake==		==Mechanism of uptake==
-	{{STRUCTURE_2axc \| PDB=2axc \| SCENE= Colicin_E7/Cole7t/1 }}

	The receptor binding domain of ColE7 binds to the [[BtuB]] vitamin B12 receptor on the outer membrane of the target cell. This causes the dissociation of the immunity protein from the cytotoxic domain of the colicin, allowing it to target the cell when it enters. Binding BtuB triggers the recruitment of [[OmpF]] and the TolQRAB complex, to bind to the translocation domain and translocate the colicin across the membrane. The mechanism by which this occurs has not yet been identified. This structure shows the translocation domain of ColE7.		The receptor binding domain of ColE7 binds to the [[BtuB]] vitamin B12 receptor on the outer membrane of the target cell. This causes the dissociation of the immunity protein from the cytotoxic domain of the colicin, allowing it to target the cell when it enters. Binding BtuB triggers the recruitment of [[OmpF]] and the TolQRAB complex, to bind to the translocation domain and translocate the colicin across the membrane. The mechanism by which this occurs has not yet been identified. This structure shows the translocation domain of ColE7.

	==Killing Activities==		==Killing Activities==
-	{{STRUCTURE_1pt3 \| PDB=1pt3 \| SCENE= Colicin_E7/Cole7cmono/1 }}

-	Colicin E7 has an endonuclease domain that degrades the DNA of the targeted cell, containing an [[H-N-H motif]]. This structure shows <scene name='Colicin_E7/Cole7c/1'>this domain</scene> bound to a <scene name='Colicin_E7/Dnaoctomer/1'>DNA octamer</scene><ref> PMID: 14962381 </ref>.	+
		+	<scene name='Colicin_E7/Cole7cmono/1'>Colicin E7</scene> has an endonuclease domain that degrades the DNA of the targeted cell, containing an [[H-N-H motif]]. This structure shows <scene name='Colicin_E7/Cole7c/1'>this domain</scene> bound to a <scene name='Colicin_E7/Dnaoctomer/1'>DNA octamer</scene><ref> PMID: 14962381 </ref>.

	The endonuclease domain is likely to use Zn<sup>2+</sup><ref> PMID: 11917029 </ref> in the DNA cleavage, although other literature does not support this<ref> PMID: 15190054 </ref>. For cleavage of single stranded DNA or RNA ColE7 can either use Ni<sup>2+</sup> or Co<sup>2+</sup><ref>PMID: 12162738</ref><ref>PMID: 11733993 </ref>. The bound ion binds to the second histadine residue in the HNH motif and stabilises the reaction.		The endonuclease domain is likely to use Zn<sup>2+</sup><ref> PMID: 11917029 </ref> in the DNA cleavage, although other literature does not support this<ref> PMID: 15190054 </ref>. For cleavage of single stranded DNA or RNA ColE7 can either use Ni<sup>2+</sup> or Co<sup>2+</sup><ref>PMID: 12162738</ref><ref>PMID: 11733993 </ref>. The bound ion binds to the second histadine residue in the HNH motif and stabilises the reaction.

Gemma McGoldrick: /* Killing Activities */

Gemma McGoldrick — Sat, 26 Feb 2011 14:53:22 GMT

Killing Activities

←Older revision		Revision as of 14:53, 26 February 2011
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	{{STRUCTURE_1pt3 \| PDB=1pt3 \| SCENE= Colicin_E7/Cole7cmono/1 }}		{{STRUCTURE_1pt3 \| PDB=1pt3 \| SCENE= Colicin_E7/Cole7cmono/1 }}

-	Colicin E7 has an endonuclease domain that degrades the DNA of the targeted cell, containing an [[H-N-H motif]]. This structure shows this domain bound to a <scene name='Colicin_E7/Dnaoctomer/1'>DNA octamer</scene><ref> PMID: 14962381 </ref>.	+	Colicin E7 has an endonuclease domain that degrades the DNA of the targeted cell, containing an [[H-N-H motif]]. This structure shows <scene name='Colicin_E7/Cole7c/1'>this domain</scene> bound to a <scene name='Colicin_E7/Dnaoctomer/1'>DNA octamer</scene><ref> PMID: 14962381 </ref>.

	The endonuclease domain is likely to use Zn<sup>2+</sup><ref> PMID: 11917029 </ref> in the DNA cleavage, although other literature does not support this<ref> PMID: 15190054 </ref>. For cleavage of single stranded DNA or RNA ColE7 can either use Ni<sup>2+</sup> or Co<sup>2+</sup><ref>PMID: 12162738</ref><ref>PMID: 11733993 </ref>. The bound ion binds to the second histadine residue in the HNH motif and stabilises the reaction.		The endonuclease domain is likely to use Zn<sup>2+</sup><ref> PMID: 11917029 </ref> in the DNA cleavage, although other literature does not support this<ref> PMID: 15190054 </ref>. For cleavage of single stranded DNA or RNA ColE7 can either use Ni<sup>2+</sup> or Co<sup>2+</sup><ref>PMID: 12162738</ref><ref>PMID: 11733993 </ref>. The bound ion binds to the second histadine residue in the HNH motif and stabilises the reaction.

Gemma McGoldrick: /* Killing Activities */

Gemma McGoldrick — Sat, 26 Feb 2011 14:51:30 GMT

Killing Activities

←Older revision		Revision as of 14:51, 26 February 2011
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	{{STRUCTURE_1pt3 \| PDB=1pt3 \| SCENE= Colicin_E7/Cole7cmono/1 }}		{{STRUCTURE_1pt3 \| PDB=1pt3 \| SCENE= Colicin_E7/Cole7cmono/1 }}

-	Colicin E7 has an endonuclease domain that degrades the DNA of the targeted cell, containing an [[H-N-H motif]]. This structure shows this domain bound to a DNA octamer <ref> PMID: 14962381 </ref>.	+	Colicin E7 has an endonuclease domain that degrades the DNA of the targeted cell, containing an [[H-N-H motif]]. This structure shows this domain bound to a <scene name='Colicin_E7/Dnaoctomer/1'>DNA octamer</scene><ref> PMID: 14962381 </ref>.

	The endonuclease domain is likely to use Zn<sup>2+</sup><ref> PMID: 11917029 </ref> in the DNA cleavage, although other literature does not support this<ref> PMID: 15190054 </ref>. For cleavage of single stranded DNA or RNA ColE7 can either use Ni<sup>2+</sup> or Co<sup>2+</sup><ref>PMID: 12162738</ref><ref>PMID: 11733993 </ref>. The bound ion binds to the second histadine residue in the HNH motif and stabilises the reaction.		The endonuclease domain is likely to use Zn<sup>2+</sup><ref> PMID: 11917029 </ref> in the DNA cleavage, although other literature does not support this<ref> PMID: 15190054 </ref>. For cleavage of single stranded DNA or RNA ColE7 can either use Ni<sup>2+</sup> or Co<sup>2+</sup><ref>PMID: 12162738</ref><ref>PMID: 11733993 </ref>. The bound ion binds to the second histadine residue in the HNH motif and stabilises the reaction.

Gemma McGoldrick at 14:40, 26 February 2011

Gemma McGoldrick — Sat, 26 Feb 2011 14:40:12 GMT

←Older revision		Revision as of 14:40, 26 February 2011
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-	Colicin E7 is a type of [[Colicin]], a bacteriocin made by ''E. coli'' which acts against other nearby ''E. coli'' to kill them with its [[DNase ~~activity~~]]; it digests the cell's genome in specific locations, ultimately leading to the death of the cell.	+	Colicin E7 is a type of [[Colicin]], a bacteriocin made by ''E. coli'' which acts against other nearby ''E. coli'' to kill them with its [[DNase Activity]]; it digests the cell's genome in specific locations, ultimately leading to the death of the cell.

	==Synthesis and release==		==Synthesis and release==

Gemma McGoldrick: /* Killing Activities */

Gemma McGoldrick — Sat, 26 Feb 2011 14:39:51 GMT

Killing Activities

←Older revision		Revision as of 14:39, 26 February 2011
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	==Killing Activities==		==Killing Activities==
-	{{STRUCTURE_1pt3 \| PDB=1pt3 \| SCENE= }}	+	{{STRUCTURE_1pt3 \| PDB=1pt3 \| SCENE= Colicin_E7/Cole7cmono/1 }}

	Colicin E7 has an endonuclease domain that degrades the DNA of the targeted cell, containing an [[H-N-H motif]]. This structure shows this domain bound to a DNA octamer <ref> PMID: 14962381 </ref>.		Colicin E7 has an endonuclease domain that degrades the DNA of the targeted cell, containing an [[H-N-H motif]]. This structure shows this domain bound to a DNA octamer <ref> PMID: 14962381 </ref>.

-	The endonuclease domain is likely to use Zn<sup>2+</sup><ref> PMID: 11917029 </ref> in the DNA cleavage, although other literature does not support this<ref> PMID: 15190054 </ref>. For cleavage of single stranded DNA or RNA ColE7 can either use Ni<sup>2+</sup> or Co<sup>2+</sup><ref>PMID: 12162738</ref><ref>PMID: 11733993 </ref>. The bound ion binds to the second histadine residue in the HNH motif and stabilises the reaction.	+	The endonuclease domain is likely to use Zn<sup>2+</sup><ref> PMID: 11917029 </ref> in the DNA cleavage, although other literature does not support this<ref> PMID: 15190054 </ref>. For cleavage of single stranded DNA or RNA ColE7 can either use Ni<sup>2+</sup> or Co<sup>2+</sup><ref>PMID: 12162738</ref><ref>PMID: 11733993 </ref>. The bound ion binds to the second histadine residue in the HNH motif and stabilises the reaction.

	==References==		==References==

	<references/>		<references/>

Gemma McGoldrick at 14:37, 26 February 2011

Gemma McGoldrick — Sat, 26 Feb 2011 14:37:23 GMT

←Older revision		Revision as of 14:37, 26 February 2011
Line 1:		Line 1:
-	Colicin E7 is a type of [[Colicin]], a bacteriocin made by ''E. coli'' which acts against other nearby ''E. coli'' to kill them with its DNase activity; it digests the cell's genome in specific locations, ultimately leading to the death of the cell.	+	Colicin E7 is a type of [[Colicin]], a bacteriocin made by ''E. coli'' which acts against other nearby ''E. coli'' to kill them with its [[DNase activity]]; it digests the cell's genome in specific locations, ultimately leading to the death of the cell.

	==Synthesis and release==		==Synthesis and release==
-	After synthesis inside the ''E. coli'' cell, the colicin binds its ~~immunity protein~~, [[Im7]], to its nuclease domain, to prevent the host cell from being killed by its activity. This immunity protein is released only on binding to the outer membrane of the target cell.	+	After synthesis inside the ''E. coli'' cell, the colicin binds its [[Colicin Immunity Protein]], [[Im7]], to its nuclease domain, to prevent the host cell from being killed by its activity. This immunity protein is released only on binding to the outer membrane of the target cell.

	==Mechanism of uptake==		==Mechanism of uptake==
	{{STRUCTURE_2axc \| PDB=2axc \| SCENE= Colicin_E7/Cole7t/1 }}		{{STRUCTURE_2axc \| PDB=2axc \| SCENE= Colicin_E7/Cole7t/1 }}
-	This structure shows the translocation domain of ColE7.	+
		+	The receptor binding domain of ColE7 binds to the [[BtuB]] vitamin B12 receptor on the outer membrane of the target cell. This causes the dissociation of the immunity protein from the cytotoxic domain of the colicin, allowing it to target the cell when it enters. Binding BtuB triggers the recruitment of [[OmpF]] and the TolQRAB complex, to bind to the translocation domain and translocate the colicin across the membrane. The mechanism by which this occurs has not yet been identified. This structure shows the translocation domain of ColE7.

	==Killing Activities==		==Killing Activities==

Gemma McGoldrick: /* Mechanism of uptake */

Gemma McGoldrick — Fri, 25 Feb 2011 13:34:37 GMT

Mechanism of uptake

←Older revision		Revision as of 13:34, 25 February 2011
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	==Mechanism of uptake==		==Mechanism of uptake==
-	{{STRUCTURE_2axc \| PDB=2axc \| SCENE= }}	+	{{STRUCTURE_2axc \| PDB=2axc \| SCENE= Colicin_E7/Cole7t/1 }}
	This structure shows the translocation domain of ColE7.		This structure shows the translocation domain of ColE7.

Gemma McGoldrick: /* Mechanism of uptake */

Gemma McGoldrick — Fri, 11 Feb 2011 13:29:00 GMT

Mechanism of uptake

←Older revision		Revision as of 13:29, 11 February 2011
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	==Mechanism of uptake==		==Mechanism of uptake==
		+	{{STRUCTURE_2axc \| PDB=2axc \| SCENE= }}
		+	This structure shows the translocation domain of ColE7.

	==Killing Activities==		==Killing Activities==

Gemma McGoldrick at 11:31, 6 February 2011

Gemma McGoldrick — Sun, 06 Feb 2011 11:31:38 GMT

←Older revision		Revision as of 11:31, 6 February 2011
Line 1:		Line 1:
-	Colicin E3 is a type of [[Colicin]], a bacteriocin made by ''E. coli'' which acts against other nearby ''E. coli'' to kill them with its DNase activity; it digests the cell's genome in specific locations, ultimately leading to the death of the cell.	+	Colicin E7 is a type of [[Colicin]], a bacteriocin made by ''E. coli'' which acts against other nearby ''E. coli'' to kill them with its DNase activity; it digests the cell's genome in specific locations, ultimately leading to the death of the cell.

	==Synthesis and release==		==Synthesis and release==
Line 9:		Line 9:
	{{STRUCTURE_1pt3 \| PDB=1pt3 \| SCENE= }}		{{STRUCTURE_1pt3 \| PDB=1pt3 \| SCENE= }}

-	Colicin E7 has an endonuclease domain that degrades the DNA of the targeted cell. This structure shows this domain bound to a DNA octamer <ref> PMID: 14962381 </ref>.	+	Colicin E7 has an endonuclease domain that degrades the DNA of the targeted cell, containing an [[H-N-H motif]]. This structure shows this domain bound to a DNA octamer <ref> PMID: 14962381 </ref>.
		+
		+	The endonuclease domain is likely to use Zn<sup>2+</sup><ref> PMID: 11917029 </ref> in the DNA cleavage, although other literature does not support this<ref> PMID: 15190054 </ref>. For cleavage of single stranded DNA or RNA ColE7 can either use Ni<sup>2+</sup> or Co<sup>2+</sup><ref>PMID: 12162738</ref><ref>PMID: 11733993 </ref>. The bound ion binds to the second histadine residue in the HNH motif and stabilises the reaction.

	==References==		==References==

	<references/>		<references/>