Colicin Immunity Protein 9 - Revision history

Colicin Immunity Protein 9 - Revision history http://52.214.119.220/wiki/index.php?title=Colicin_Immunity_Protein_9&action=history Revision history for this page on the wiki en MediaWiki 1.11.2 Wed, 08 Apr 2026 16:42:56 GMT Michal Harel at 09:27, 1 February 2016 http://52.214.119.220/wiki/index.php?title=Colicin_Immunity_Protein_9&diff=2526854&oldid=prev <p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 09:27, 1 February 2016</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><StructureSection load="1bxi" size="<del style="color: red; font-weight: bold; text-decoration: none;">400</del>" color="" frame="true" spin="on" Scene= <del style="color: red; font-weight: bold; text-decoration: none;">align</del>="right" caption= ></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><StructureSection load="1bxi" size="<ins style="color: red; font-weight: bold; text-decoration: none;">350</ins>" color="" frame="true" spin="on" Scene= <ins style="color: red; font-weight: bold; text-decoration: none;">side</ins>="right" caption=<ins style="color: red; font-weight: bold; text-decoration: none;">'E. coli colicin immunity protein 9 (grey) complex with colicin 9 DNase domain (green), phosphate and Ni+2 ion (PDB code [[1bxi]])' </ins>></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> </table> Mon, 01 Feb 2016 09:27:32 GMT Michal Harel http://52.214.119.220/wiki/index.php/Talk:Colicin_Immunity_Protein_9 Michal Harel at 07:34, 1 August 2012 http://52.214.119.220/wiki/index.php?title=Colicin_Immunity_Protein_9&diff=1512402&oldid=prev <p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 07:34, 1 August 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><StructureSection load="1bxi" size="400" color="" frame="true" spin="on" Scene= align="right" caption= ></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><StructureSection load="1bxi" size="400" color="" frame="true" spin="on" Scene= align="right" caption= ></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">{{STRUCTURE_1bxi| PDB=1bxi | SIZE=400| SCENE= |right|CAPTION=Colicin-E9 immunity protein complex with colicin E9 DNase domain, [[1bxi]] }}</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:1bxi.png|left|200px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:1bxi.png|left|200px]]</div></td></tr> </table> Wed, 01 Aug 2012 07:34:31 GMT Michal Harel http://52.214.119.220/wiki/index.php/Talk:Colicin_Immunity_Protein_9 Michal Harel at 07:33, 1 August 2012 http://52.214.119.220/wiki/index.php?title=Colicin_Immunity_Protein_9&diff=1512401&oldid=prev <p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 07:33, 1 August 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><StructureSection load="1bxi" size="400" color="" frame="true" spin="on" Scene= align="right" caption= ></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><StructureSection load="1bxi" size="400" color="" frame="true" spin="on" Scene= align="right" caption= ></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{{STRUCTURE_1bxi| PDB=1bxi | SIZE=400| SCENE= |right|CAPTION=Colicin-E9 immunity protein complex with colicin E9 DNase domain, [[1bxi]] }}</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:1bxi.png|left|200px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:1bxi.png|left|200px]]</div></td></tr> </table> Wed, 01 Aug 2012 07:33:19 GMT Michal Harel http://52.214.119.220/wiki/index.php/Talk:Colicin_Immunity_Protein_9 Alexander Berchansky at 09:12, 13 June 2012 http://52.214.119.220/wiki/index.php?title=Colicin_Immunity_Protein_9&diff=1407266&oldid=prev <p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 09:12, 13 June 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 14:</td> <td colspan="2" class="diff-lineno">Line 14:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></StructureSection></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></StructureSection></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==About this Structure==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==About this Structure==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">7CEI </del>is a 2 <del style="color: red; font-weight: bold; text-decoration: none;">chains </del>structure of <del style="color: red; font-weight: bold; text-decoration: none;">sequences </del>from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=<del style="color: red; font-weight: bold; text-decoration: none;">7CEI </del>OCA]. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[1bxi]] </ins>is a 2 <ins style="color: red; font-weight: bold; text-decoration: none;">chain </ins>structure of <ins style="color: red; font-weight: bold; text-decoration: none;">[[Colicin]] and [[Colicin Immunity Protein]] with sequence </ins>from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=<ins style="color: red; font-weight: bold; text-decoration: none;">1BXI </ins>OCA]. </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==See also==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==See also==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Colicin Immunity Protein]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Colicin Immunity Protein]]</div></td></tr> </table> Wed, 13 Jun 2012 09:12:07 GMT Alexander Berchansky http://52.214.119.220/wiki/index.php/Talk:Colicin_Immunity_Protein_9 Alexander Berchansky: New page: <StructureSection load="1bxi" size="400" color="" frame="true" spin="on" Scene= align="right" caption= > 200px {{ABSTRACT_PUBMED_010074943}} {{Clear}} <scene n... http://52.214.119.220/wiki/index.php?title=Colicin_Immunity_Protein_9&diff=1407265&oldid=prev <p>New page: <StructureSection load="1bxi" size="400" color="" frame="true" spin="on" Scene= align="right" caption= > <a href="/wiki/index.php/Image:1bxi.png" title="Image:1bxi.png">200px</a> {{ABSTRACT_PUBMED_010074943}} {{Clear}} <scene n...</p> <p><b>New page</b></p><div><StructureSection load="1bxi" size="400" color="" frame="true" spin="on" Scene= align="right" caption= ><br /> <br /> [[Image:1bxi.png|left|200px]]<br /> <br /> {{ABSTRACT_PUBMED_010074943}}<br /> <br /> {{Clear}}<br /> <scene name='3gkl/Align/2'>Structural alignment</scene> of the immunity protein 9 (Im9, [[1bxi]], <span style="color:yellow;background-color:black;font-weight:bold;">colored yellow</span>), <span style="color:lime;background-color:black;font-weight:bold;">evolved variant R12-2 (lime)</span>, and <font color='blue'><b>immunity protein 7 (Im7, colored blue</b></font>, [[7cei]]) reveals their structural identity. However, when the immunity proteins-bound <scene name='3gkl/Align/3'>colicins within their complexes were aligned</scene>, they demonstrate somewhat different picture. The Im9 and Im7 are differ more in their binding configurations (19°, with Tyr54-Tyr55 as the pivot), while the variant R12-2 is in an intermediate configuration between Im9 and Im7. Of note, in the variant R12-2 (3gkl) and Im9 ([[1bxi]]) there are Tyr54 and Tyr55, while in the Im7 ([[7cei]]) Tyr55 and Tyr56 are homologous to them. The most <scene name='3gkl/Align/4'>prominent differences</scene> are in the loop between helices α1 and α2 in Im9 (yellow, labeled in black) and <span style="color:lime;background-color:black;font-weight:bold;">evolved variant R12-2 (lime, labeled in black)</span>. This loop consists of three mutations: N24D, T27A, and S28T in variant R12-2. We can see the deviations in the relative position of helices α1 and α2, in the loop's backbone and in the side chains of residues 24, 26 and 28.<br /> <br /> {{Clear}}<br /> Comparison of the different Im-colicin complexes reveals changes in the binding configuration of the evolved variants which increase affinity toward ColE7 by re-aligning pre-existing Im9 residues. Glu30 of Im9 ([[1bxi]], <span style="color:yellow;background-color:black;font-weight:bold;">colored yellow</span>) forms <scene name='3gkl/Active_site/3'>double salt bridge</scene> with Arg54 of <span style="color:orange;background-color:black;font-weight:bold;">ColE9 (orange)</span>, whereas Asp51 have not direct side chain–side chain interactions. <font color='blue'><b>Asp31 of Im7 (blue)</b></font> which is corresponding to Im9 Glu30 is involved in <scene name='3gkl/Active_site/6'>cluster of salt bridge bonds</scene> to <font color='darkmagenta'><b>Arg520 and Lys525 of ColE7 (darkmagenta)</b></font>, while <font color='blue'><b>Asp52 of Im7</b></font> (corresponding to Im9 Asp51) is within hydrogen bond distance to <font color='darkmagenta'><b>Thr531 and Arg530 of ColE7</b></font> ([[7cei]]). <span style="color:lime;background-color:black;font-weight:bold;">Glu30 in the variant R12-2 (lime)</span> is shifted and forms a <scene name='3gkl/Active_site/8'>double salt bridge</scene> to <font color='magenta'><b>Arg520 of ColE7 (magenta)</b></font>. <span style="color:lime;background-color:black;font-weight:bold;">Asp51</span> is within hydrogen bond distance to <font color='magenta'><b>Thr531 of ColE7</b></font> ([[3gkl]]). However, the side chains of <font color='magenta'><b>Lys525 and Arg530</b></font>, which are very important in salt bridge contacts with Glu30 and Asp51, respectively, in the structure of the ColE7–Im7 complex have a different conformation that eliminates these contacts in evolved variant R12-2.<br /> <br /> {{Clear}} <br /> </StructureSection><br /> ==About this Structure==<br /> 7CEI is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CEI OCA]. <br /> ==See also==<br /> *[[Colicin Immunity Protein]]<br /> ==References==<br /> <ref group="xtra">PMID:010074943</ref> <ref group='xtra'>PMID:19749752</ref> <references group="xtra"/><br /> [[Category: Escherichia coli]]<br /> [[Category: Hemmings, A M.]]<br /> [[Category: James, R.]]<br /> [[Category: Kleanthous, C.]]<br /> [[Category: Kuhlmann, U C.]]<br /> [[Category: Moore, G R.]]<br /> [[Category: Colicin]]<br /> [[Category: Endonuclease]]<br /> [[Category: Immune system]]<br /> [[Category: Protein-protein interaction]]</div> Wed, 13 Jun 2012 09:09:26 GMT Alexander Berchansky http://52.214.119.220/wiki/index.php/Talk:Colicin_Immunity_Protein_9