Glutamate synthase - Revision history

Michal Harel at 10:27, 14 March 2023

Michal Harel — Tue, 14 Mar 2023 10:27:22 GMT

←Older revision		Revision as of 10:27, 14 March 2023
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	**[[6s6s]], [[6s6t]], [[6s6u]], [[6s6x]] – AbGS α+β subunits + FAD + FMN + Fe3S4 + Fe4S4 – CryoEM<br />		**[[6s6s]], [[6s6t]], [[6s6u]], [[6s6x]] – AbGS α+β subunits + FAD + FMN + Fe3S4 + Fe4S4 – CryoEM<br />
	**[[2vdc]] – AbGS α+β subunits + FAD + FMN + Fe3S4 + Fe4S4 + oxoglutarate – CryoEM<br />		**[[2vdc]] – AbGS α+β subunits + FAD + FMN + Fe3S4 + Fe4S4 + oxoglutarate – CryoEM<br />
		+	**[[7mfm]], [[7mft]] – GS + glutamate dehydrogenase – ''Bacillus subtilis'' - CryoEM<br />

	*Fd-dependent glutamate synthase		*Fd-dependent glutamate synthase

Michal Harel at 07:21, 4 August 2020

Michal Harel — Tue, 04 Aug 2020 07:21:45 GMT

←Older revision		Revision as of 07:21, 4 August 2020
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	**[[1ea0]] – AbGS α subunit + FMN + oxo-glutarate – ''Azospirillum brasilense''<br />		**[[1ea0]] – AbGS α subunit + FMN + oxo-glutarate – ''Azospirillum brasilense''<br />
-	**[[2vdc]] – AbGS α+β subunits + FAD + FMN + oxoglutarate – CryoEM<br />	+	**[[6s6s]], [[6s6t]], [[6s6u]], [[6s6x]] – AbGS α+β subunits + FAD + FMN + Fe3S4 + Fe4S4 – CryoEM<br />
		+	**[[2vdc]] – AbGS α+β subunits + FAD + FMN + Fe3S4 + Fe4S4 + oxoglutarate – CryoEM<br />

	*Fd-dependent glutamate synthase		*Fd-dependent glutamate synthase

Alexander Berchansky at 13:31, 1 April 2019

Alexander Berchansky — Mon, 01 Apr 2019 13:31:02 GMT

←Older revision		Revision as of 13:31, 1 April 2019
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	== Structural highlights ==		== Structural highlights ==

-	The <scene name='72/727110/Cv/6'>Fd-GS structure contains 4 domains</scene>. The N-terminal domain is an amidotransferase domain and contains an active site where <scene name='72/727110/Cv/3'>residue Cys1</scene> catalyzes the hydrolysis of glutamine to glutarate; a core domain; an FMN-binding domain which <scene name='72/727110/Cv/5'>contains an Fe3S4 cluster</scene> and reduces the intermediate iminoglutarate to 2-oxoglutarate and produces a second molecule of glutarate and a C-terminal domain. <scene name='72/727110/Cv/7'>Residue M475 is located between the FMN and the Fe3S4 cluster</scene>. It is is strictly conserved and may perform the electron transfer between the two centers. <scene name='72/727110/Cv/8'>FMN binding site</scene> The <scene name='72/727110/Cv/9'>2-oxoglutarate binds at the FMN-binding domain</scene><ref>PMID:11967268</ref>.	+	The <scene name='72/727110/Cv/10'>Fd-GS structure contains 4 domains</scene>. The N-terminal domain is an amidotransferase domain and contains an active site where <scene name='72/727110/Cv/11'>residue Cys1</scene> catalyzes the hydrolysis of glutamine to glutarate; a core domain; an FMN-binding domain which <scene name='72/727110/Cv/12'>contains an Fe3S4 cluster</scene> and reduces the intermediate iminoglutarate to 2-oxoglutarate and produces a second molecule of glutarate and a C-terminal domain. <scene name='72/727110/Cv/13'>Residue M475 is located between the FMN and the Fe3S4 cluster</scene>. It is is strictly conserved and may perform the electron transfer between the two centers. <scene name='72/727110/Cv/14'>FMN binding site</scene> The <scene name='72/727110/Cv/15'>2-oxoglutarate binds at the FMN-binding domain</scene><ref>PMID:11967268</ref>.

	</StructureSection>		</StructureSection>

Joel L. Sussman at 11:30, 20 October 2017

Joel L. Sussman — Fri, 20 Oct 2017 11:30:24 GMT

←Older revision		Revision as of 11:30, 20 October 2017
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-	<StructureSection load='~~1llw~~' size='350' side='right' caption='Ferredoxin-dependent glutamate synthase containing a Fe3S4 cluster complex with FMN, 2-oxoglutarate (PDB code [[1llw]])' scene='72/727110/Cv/1'>	+	<StructureSection load='' size='350' side='right' caption='Ferredoxin-dependent glutamate synthase containing a Fe3S4 cluster complex with FMN, 2-oxoglutarate (PDB code [[1llw]])' scene='72/727110/Cv/1'>
	== Function ==		== Function ==
	'''Glutamate synthase''' (GS) is an iron-sulfur flavoprotein which catalyzes the reverse reaction which converts L-glutamine, 2-oxoglutarate and NADPH to L-glutarate and NADP.		'''Glutamate synthase''' (GS) is an iron-sulfur flavoprotein which catalyzes the reverse reaction which converts L-glutamine, 2-oxoglutarate and NADPH to L-glutarate and NADP.

Michal Harel at 20:54, 19 October 2017

Michal Harel — Thu, 19 Oct 2017 20:54:28 GMT

←Older revision		Revision as of 20:54, 19 October 2017
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	== References ==		== References ==
	<references/>		<references/>
		+	[[Category:Topic Page]]

Alexander Berchansky at 12:46, 14 March 2016

Alexander Berchansky — Mon, 14 Mar 2016 12:46:01 GMT

←Older revision		Revision as of 12:46, 14 March 2016
Line 6:		Line 6:
	== Structural highlights ==		== Structural highlights ==

-	The <scene name='72/727110/Cv/6'>Fd-GS structure contains 4 domains</scene>. The N-terminal domain is an amidotransferase domain and contains an active site where <scene name='72/727110/Cv/3'>residue Cys1</scene> catalyzes the hydrolysis of glutamine to glutarate; a core domain; an FMN-binding domain which <scene name='72/727110/Cv/5'>contains an Fe3S4 cluster</scene> and reduces the intermediate iminoglutarate to 2-oxoglutarate and produces a second molecule of glutarate and a C-terminal domain. <scene name='72/727110/Cv/7'>Residue M475 is located between the FMN and the Fe3S4 cluster</scene>. It is is strictly conserved and may perform the electron transfer between the two centers. The 2-oxoglutarate binds at the FMN-binding domain<ref>PMID:11967268</ref>.	+	The <scene name='72/727110/Cv/6'>Fd-GS structure contains 4 domains</scene>. The N-terminal domain is an amidotransferase domain and contains an active site where <scene name='72/727110/Cv/3'>residue Cys1</scene> catalyzes the hydrolysis of glutamine to glutarate; a core domain; an FMN-binding domain which <scene name='72/727110/Cv/5'>contains an Fe3S4 cluster</scene> and reduces the intermediate iminoglutarate to 2-oxoglutarate and produces a second molecule of glutarate and a C-terminal domain. <scene name='72/727110/Cv/7'>Residue M475 is located between the FMN and the Fe3S4 cluster</scene>. It is is strictly conserved and may perform the electron transfer between the two centers. <scene name='72/727110/Cv/8'>FMN binding site</scene> The <scene name='72/727110/Cv/9'>2-oxoglutarate binds at the FMN-binding domain</scene><ref>PMID:11967268</ref>.

	</StructureSection>		</StructureSection>

Alexander Berchansky at 12:35, 14 March 2016

Alexander Berchansky — Mon, 14 Mar 2016 12:35:23 GMT

←Older revision		Revision as of 12:35, 14 March 2016
Line 6:		Line 6:
	== Structural highlights ==		== Structural highlights ==

-	The <scene name='72/727110/Cv/6'>Fd-GS structure contains 4 domains</scene>. The N-terminal domain is an amidotransferase domain and contains an active site where <scene name='72/727110/Cv/3'>residue Cys1</scene> catalyzes the hydrolysis of glutamine to glutarate; a core domain; an FMN-binding domain which <scene name='72/727110/Cv/5'>contains an Fe3S4 cluster</scene> and reduces the intermediate iminoglutarate to 2-oxoglutarate and produces a second molecule of glutarate and a C-terminal domain. Residue M475 is located between the FMN and the Fe3S4 cluster. It is is strictly conserved and may perform the electron transfer between the two centers. The 2-oxoglutarate binds at the FMN-binding domain<ref>PMID:11967268</ref>.	+	The <scene name='72/727110/Cv/6'>Fd-GS structure contains 4 domains</scene>. The N-terminal domain is an amidotransferase domain and contains an active site where <scene name='72/727110/Cv/3'>residue Cys1</scene> catalyzes the hydrolysis of glutamine to glutarate; a core domain; an FMN-binding domain which <scene name='72/727110/Cv/5'>contains an Fe3S4 cluster</scene> and reduces the intermediate iminoglutarate to 2-oxoglutarate and produces a second molecule of glutarate and a C-terminal domain. <scene name='72/727110/Cv/7'>Residue M475 is located between the FMN and the Fe3S4 cluster</scene>. It is is strictly conserved and may perform the electron transfer between the two centers. The 2-oxoglutarate binds at the FMN-binding domain<ref>PMID:11967268</ref>.

	</StructureSection>		</StructureSection>

Alexander Berchansky at 12:31, 14 March 2016

Alexander Berchansky — Mon, 14 Mar 2016 12:31:13 GMT

←Older revision		Revision as of 12:31, 14 March 2016
Line 6:		Line 6:
	== Structural highlights ==		== Structural highlights ==

-	The <scene name='72/727110/Cv/2'>Fd-GS structure contains 4 domains</scene>. The N-terminal domain is an amidotransferase domain and contains an active site where <scene name='72/727110/Cv/3'>residue Cys1</scene> catalyzes the hydrolysis of glutamine to glutarate; a core domain; an FMN-binding domain which <scene name='72/727110/Cv/5'>contains an Fe3S4 cluster</scene> and reduces the intermediate iminoglutarate to 2-oxoglutarate and produces a second molecule of glutarate and a C-terminal domain. Residue M475 is located between the FMN and the Fe3S4 cluster. It is is strictly conserved and may perform the electron transfer between the two centers. The 2-oxoglutarate binds at the FMN-binding domain<ref>PMID:11967268</ref>.	+	The <scene name='72/727110/Cv/6'>Fd-GS structure contains 4 domains</scene>. The N-terminal domain is an amidotransferase domain and contains an active site where <scene name='72/727110/Cv/3'>residue Cys1</scene> catalyzes the hydrolysis of glutamine to glutarate; a core domain; an FMN-binding domain which <scene name='72/727110/Cv/5'>contains an Fe3S4 cluster</scene> and reduces the intermediate iminoglutarate to 2-oxoglutarate and produces a second molecule of glutarate and a C-terminal domain. Residue M475 is located between the FMN and the Fe3S4 cluster. It is is strictly conserved and may perform the electron transfer between the two centers. The 2-oxoglutarate binds at the FMN-binding domain<ref>PMID:11967268</ref>.

	</StructureSection>		</StructureSection>

Alexander Berchansky at 12:29, 14 March 2016

Alexander Berchansky — Mon, 14 Mar 2016 12:29:45 GMT

←Older revision		Revision as of 12:29, 14 March 2016
Line 6:		Line 6:
	== Structural highlights ==		== Structural highlights ==

-	The <scene name='72/727110/Cv/2'>Fd-GS structure contains 4 domains</scene>. The N-terminal domain is an amidotransferase domain and contains an active site where <scene name='72/727110/Cv/3'>residue Cys1</scene> catalyzes the hydrolysis of glutamine to glutarate; a core domain; an FMN-binding domain which contains an Fe3S4 cluster and reduces the intermediate iminoglutarate to 2-oxoglutarate and produces a second molecule of glutarate and a C-terminal domain. Residue M475 is located between the FMN and the Fe3S4 cluster. It is is strictly conserved and may perform the electron transfer between the two centers. The 2-oxoglutarate binds at the FMN-binding domain<ref>PMID:11967268</ref>.	+	The <scene name='72/727110/Cv/2'>Fd-GS structure contains 4 domains</scene>. The N-terminal domain is an amidotransferase domain and contains an active site where <scene name='72/727110/Cv/3'>residue Cys1</scene> catalyzes the hydrolysis of glutamine to glutarate; a core domain; an FMN-binding domain which <scene name='72/727110/Cv/5'>contains an Fe3S4 cluster</scene> and reduces the intermediate iminoglutarate to 2-oxoglutarate and produces a second molecule of glutarate and a C-terminal domain. Residue M475 is located between the FMN and the Fe3S4 cluster. It is is strictly conserved and may perform the electron transfer between the two centers. The 2-oxoglutarate binds at the FMN-binding domain<ref>PMID:11967268</ref>.

	</StructureSection>		</StructureSection>

Alexander Berchansky at 11:53, 14 March 2016

Alexander Berchansky — Mon, 14 Mar 2016 11:53:21 GMT

←Older revision		Revision as of 11:53, 14 March 2016
Line 6:		Line 6:
	== Structural highlights ==		== Structural highlights ==

-	The <scene name='72/727110/Cv/2'>Fd-GS structure contains 4 domains</scene>. The N-terminal domain is an amidotransferase domain and contains an active site where residue Cys1 catalyzes the hydrolysis of glutamine to glutarate; a core domain; an FMN-binding domain which contains an Fe3S4 cluster and reduces the intermediate iminoglutarate to 2-oxoglutarate and produces a second molecule of glutarate and a C-terminal domain. Residue M475 is located between the FMN and the Fe3S4 cluster. It is is strictly conserved and may perform the electron transfer between the two centers. The 2-oxoglutarate binds at the FMN-binding domain<ref>PMID:11967268</ref>.	+	The <scene name='72/727110/Cv/2'>Fd-GS structure contains 4 domains</scene>. The N-terminal domain is an amidotransferase domain and contains an active site where <scene name='72/727110/Cv/3'>residue Cys1</scene> catalyzes the hydrolysis of glutamine to glutarate; a core domain; an FMN-binding domain which contains an Fe3S4 cluster and reduces the intermediate iminoglutarate to 2-oxoglutarate and produces a second molecule of glutarate and a C-terminal domain. Residue M475 is located between the FMN and the Fe3S4 cluster. It is is strictly conserved and may perform the electron transfer between the two centers. The 2-oxoglutarate binds at the FMN-binding domain<ref>PMID:11967268</ref>.

	</StructureSection>		</StructureSection>