Glutaminase - Revision history

Michal Harel at 09:38, 11 July 2019

Michal Harel — Thu, 11 Jul 2019 09:38:15 GMT

←Older revision		Revision as of 09:38, 11 July 2019
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	</StructureSection>		</StructureSection>
-	==3D structures of glutaminase==

-	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
-	{{#tree:id=OrganizedByTopic\|openlevels=0\|
-
-	*GLN
-
-	**[[3voy]], [[5d3o]] – hK-GLN – human<br />
-	**[[5u0i]], [[5u0j]] – hK-GLN C-terminal<br />
-	**[[4bqm]] - hL-GLN catalytic domain<br />
-	**[[5u0k]] – hL-GLN C-terminal<br />
-	**[[3ss3]], [[3ss4]] – mGLN C – mouse<br />
-	**[[4jkt]] - mK-GLN<br />
-	**[[3if5]], [[3ih8]], [[3ih9]], [[3agd]] – MlGLN – ''Micrococcus luteus''<br />
-	**[[2pby]] – GLN – ''Geobacillus kaustophilus''<br />
-	**[[2zk9]] – CpGLN – ''Chryseobacterium proteolyticum''<br />
-	**[[3a54]], [[3a55]], [[3a56]] – CpGLN (mutant)<br />
-	**[[2ksv]] – CpGLN - NMR<br />
-	**[[3agf]] - BsGLN – ''Bacillus subtilis''<br />
-
-	*GLN complex
-
-	**[[3czd]], [[3unw]] – hK-GLN + Glu<br />
-	**[[3vp0]] – hK-GLN + Gln<br />
-	**[[3uo9]], [[3voz]], [[3vp2]], [[3vp3]], [[3vp4]], [[4o7d]], [[5fi2]], [[5fi6]], [[5fi7]], [[5hl1]], [[5i94]], [[5jyo]], [[5jyp]], [[5wj6]], [[5uqe]] - hK-GLN + inhibitor<br />
-	**[[3vp1]] - hK-GLN + inhibitor + Glu<br />
-	**[[3iha]], [[3ihb]], [[3age]] - MlGLN + Glu<br />
-	**[[3ss5]] – mGLN C + Glu<br />
-	**[[5w2j]] - mK-GLN + peptide<br />
-	**[[3brm]], [[2osu]] – BsGLN + norleucine derivative<br />
-
-	*GLN-ASN
-
-	**[[1agx]] – GLN-ASN – ''Acinetobacter glutaminacificans''<br />
-	**[[3pga]], [[4pga]] – PsGLN-ASN – ''Pseudomonas''<br />
-	**[[1djo]], [[1djp]] - PsGLN-ASN + norvaline derivative<br />
-
-	*Other GLN
-
-	**[[1u60]] - YbaS <br />
-	**[[3brm]] - YbgJ in a complex with DON <br />
-	**[[1mki]] - YbgJ free <br />
-	}}
	== References ==		== References ==
	<references/>		<references/>
	Created with the participation of [[User:Lindsey Butler\|Lindsey Butler]].		Created with the participation of [[User:Lindsey Butler\|Lindsey Butler]].
	[[Category:Topic Page]]		[[Category:Topic Page]]

Michal Harel at 09:37, 11 July 2019

Michal Harel — Thu, 11 Jul 2019 09:37:07 GMT

←Older revision		Revision as of 09:37, 11 July 2019
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	== Structural highlights ==		== Structural highlights ==
	The glutamate binding site is in the helical domain of GLN and <scene name='49/497107/Cv/5'>Tyr residue serves as a general acid in the catalysis</scene><ref>PMID:22049910</ref>. <scene name='49/497107/Cv/6'>Whole glutamate binding site</scene>.		The glutamate binding site is in the helical domain of GLN and <scene name='49/497107/Cv/5'>Tyr residue serves as a general acid in the catalysis</scene><ref>PMID:22049910</ref>. <scene name='49/497107/Cv/6'>Whole glutamate binding site</scene>.
		+
		+	==3D structures of glutaminase==
		+	[[Glutaminase 3D structures]]
		+
	</StructureSection>		</StructureSection>
	==3D structures of glutaminase==		==3D structures of glutaminase==
Line 17:		Line 21:
	*GLN		*GLN

		+	**[[3voy]], [[5d3o]] – hK-GLN – human<br />
		+	**[[5u0i]], [[5u0j]] – hK-GLN C-terminal<br />
		+	**[[4bqm]] - hL-GLN catalytic domain<br />
		+	**[[5u0k]] – hL-GLN C-terminal<br />
		+	**[[3ss3]], [[3ss4]] – mGLN C – mouse<br />
		+	**[[4jkt]] - mK-GLN<br />
	**[[3if5]], [[3ih8]], [[3ih9]], [[3agd]] – MlGLN – ''Micrococcus luteus''<br />		**[[3if5]], [[3ih8]], [[3ih9]], [[3agd]] – MlGLN – ''Micrococcus luteus''<br />
	**[[2pby]] – GLN – ''Geobacillus kaustophilus''<br />		**[[2pby]] – GLN – ''Geobacillus kaustophilus''<br />
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	**[[2ksv]] – CpGLN - NMR<br />		**[[2ksv]] – CpGLN - NMR<br />
	**[[3agf]] - BsGLN – ''Bacillus subtilis''<br />		**[[3agf]] - BsGLN – ''Bacillus subtilis''<br />
-	**[[3ss3]], [[3ss4]] – mGLN C – mouse<br />
-	**[[4jkt]] - mK-GLN<br />
-	**[[3voy]], [[5d3o]] – hK-GLN – human<br />
-	**[[5u0i]], [[5u0j]] – hK-GLN C-terminal<br />
-	**[[4bqm]] - hL-GLN catalytic domain<br />
-	**[[5u0k]] – hL-GLN C-terminal<br />

	*GLN complex		*GLN complex

-	**[[3brm]], [[2osu]] – BsGLN + norleucine derivative<br />
	**[[3czd]], [[3unw]] – hK-GLN + Glu<br />		**[[3czd]], [[3unw]] – hK-GLN + Glu<br />
	**[[3vp0]] – hK-GLN + Gln<br />		**[[3vp0]] – hK-GLN + Gln<br />
Line 38:		Line 41:
	**[[3vp1]] - hK-GLN + inhibitor + Glu<br />		**[[3vp1]] - hK-GLN + inhibitor + Glu<br />
	**[[3iha]], [[3ihb]], [[3age]] - MlGLN + Glu<br />		**[[3iha]], [[3ihb]], [[3age]] - MlGLN + Glu<br />
-	**[[3ss5]] – mGLN + Glu<br />	+	**[[3ss5]] – mGLN C + Glu<br />
		+	**[[5w2j]] - mK-GLN + peptide<br />
		+	**[[3brm]], [[2osu]] – BsGLN + norleucine derivative<br />

	*GLN-ASN		*GLN-ASN

Alexander Berchansky at 13:35, 1 April 2019

Alexander Berchansky — Mon, 01 Apr 2019 13:35:40 GMT

←Older revision		Revision as of 13:35, 1 April 2019
Line 8:		Line 8:

	== Structural highlights ==		== Structural highlights ==
-	The glutamate binding site is in the helical domain of GLN and <scene name='49/497107/Cv/4'>Tyr residue serves as a general acid in the catalysis</scene><ref>PMID:22049910</ref>. <scene name='49/497107/Cv/3'>Whole glutamate binding site</scene>.	+	The glutamate binding site is in the helical domain of GLN and <scene name='49/497107/Cv/5'>Tyr residue serves as a general acid in the catalysis</scene><ref>PMID:22049910</ref>. <scene name='49/497107/Cv/6'>Whole glutamate binding site</scene>.
	</StructureSection>		</StructureSection>
	==3D structures of glutaminase==		==3D structures of glutaminase==

Michal Harel at 11:21, 16 January 2019

Michal Harel — Wed, 16 Jan 2019 11:21:40 GMT

←Older revision		Revision as of 11:21, 16 January 2019
Line 2:		Line 2:

	== Function ==		== Function ==
-	'''Glutaminase''' (GLN) catalyzes the conversion of glutamine (Gln) to glutamic acid (Glu). GLN is present at the axonal termini of neurons where GA functions as a neurotransmitter. ADP is an activator of GLN. GLN is a homodimer<ref>PMID:11533299</ref>. '''K-GLN''' is the kidney isoform; '''L-GLN''' is the liver isoform. In human GLN is found as 2 isozymes – GLN and GLN 2. '''Glutaminase-asparaginase''' (GLN-ASN) can amidohydrolase both glutamine and asparagine to their corresponding Glu and Asp<ref>PMID:6838661</ref>.	+	'''Glutaminase''' (GLN) catalyzes the conversion of glutamine (Gln) to glutamic acid (Glu). GLN is present at the axonal termini of neurons where GA functions as a neurotransmitter. ADP is an activator of GLN. GLN is a homodimer<ref>PMID:11533299</ref>. '''K-GLN''' is the kidney isoform; '''L-GLN''' is the liver isoform. In human GLN is found as 2 isozymes – GLN and GLN 2. '''Glutaminase-asparaginase''' (GLN-ASN) can amidohydrolase both glutamine and asparagine to their corresponding Glu and Asp<ref>PMID:6838661</ref>. For details on GLN-ASN see [[Glutaminase-Asparaginase (Pseudomonas 7A)]].

	==Relevance==		==Relevance==

Michal Harel at 06:37, 31 May 2018

Michal Harel — Thu, 31 May 2018 06:37:13 GMT

←Older revision		Revision as of 06:37, 31 May 2018
Line 2:		Line 2:

	== Function ==		== Function ==
-	'''Glutaminase''' (GLN) catalyzes the conversion of glutamine (Gln) to glutamic acid (Glu). GLN is present at the axonal termini of neurons where GA functions as a neurotransmitter. ADP is an activator of GLN. GLN is a homodimer<ref>PMID:11533299</ref>. In human GLN is found as 2 isozymes – GLN and GLN 2. '''Glutaminase-asparaginase''' (GLN-ASN) can amidohydrolase both glutamine and asparagine to their corresponding Glu and Asp<ref>PMID:6838661</ref>.	+	'''Glutaminase''' (GLN) catalyzes the conversion of glutamine (Gln) to glutamic acid (Glu). GLN is present at the axonal termini of neurons where GA functions as a neurotransmitter. ADP is an activator of GLN. GLN is a homodimer<ref>PMID:11533299</ref>. '''K-GLN''' is the kidney isoform; '''L-GLN''' is the liver isoform. In human GLN is found as 2 isozymes – GLN and GLN 2. '''Glutaminase-asparaginase''' (GLN-ASN) can amidohydrolase both glutamine and asparagine to their corresponding Glu and Asp<ref>PMID:6838661</ref>.

	==Relevance==		==Relevance==
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	**[[3agf]] - BsGLN – ''Bacillus subtilis''<br />		**[[3agf]] - BsGLN – ''Bacillus subtilis''<br />
	**[[3ss3]], [[3ss4]] – mGLN C – mouse<br />		**[[3ss3]], [[3ss4]] – mGLN C – mouse<br />
-	**[[4jkt]] - ~~mGLN kidney isoform~~<br />	+	**[[4jkt]] - mK-GLN<br />
-	**[[3voy]], [[5d3o]] – ~~hGLN kidney isoform~~ – human<br />	+	**[[3voy]], [[5d3o]] – hK-GLN – human<br />
-	**[[4bqm]] - ~~hGLN liver isoform~~<br />	+	**[[5u0i]], [[5u0j]] – hK-GLN C-terminal<br />
		+	**[[4bqm]] - hL-GLN catalytic domain<br />
		+	**[[5u0k]] – hL-GLN C-terminal<br />

	*GLN complex		*GLN complex

	**[[3brm]], [[2osu]] – BsGLN + norleucine derivative<br />		**[[3brm]], [[2osu]] – BsGLN + norleucine derivative<br />
-	**[[3czd]], [[3unw]] – ~~hGLN~~ + Glu<br />	+	**[[3czd]], [[3unw]] – hK-GLN + Glu<br />
-	**[[3vp0]] – ~~hGLN~~ + Gln<br />	+	**[[3vp0]] – hK-GLN + Gln<br />
-	**[[3uo9]], [[3voz]], [[3vp2]], [[3vp3]], [[3vp4]], [[4o7d]], [[5fi2]], [[5fi6]], [[5fi7]], [[5hl1]], [[5i94]], [[5jyo]], [[5jyp]] - ~~hGLN~~ + inhibitor<br />	+	**[[3uo9]], [[3voz]], [[3vp2]], [[3vp3]], [[3vp4]], [[4o7d]], [[5fi2]], [[5fi6]], [[5fi7]], [[5hl1]], [[5i94]], [[5jyo]], [[5jyp]], [[5wj6]], [[5uqe]] - hK-GLN + inhibitor<br />
		+	**[[3vp1]] - hK-GLN + inhibitor + Glu<br />
	**[[3iha]], [[3ihb]], [[3age]] - MlGLN + Glu<br />		**[[3iha]], [[3ihb]], [[3age]] - MlGLN + Glu<br />
	**[[3ss5]] – mGLN + Glu<br />		**[[3ss5]] – mGLN + Glu<br />
-	**[[3vp1]] - hGLN + inhibitor + Glu<br />

	*GLN-ASN		*GLN-ASN

Michal Harel at 09:03, 29 May 2017

Michal Harel — Mon, 29 May 2017 09:03:32 GMT

←Older revision		Revision as of 09:03, 29 May 2017
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	**[[3ss3]], [[3ss4]] – mGLN C – mouse<br />		**[[3ss3]], [[3ss4]] – mGLN C – mouse<br />
	**[[4jkt]] - mGLN kidney isoform<br />		**[[4jkt]] - mGLN kidney isoform<br />
-	**[[3voy]] – hGLN kidney isoform – human<br />	+	**[[3voy]], [[5d3o]] – hGLN kidney isoform – human<br />
	**[[4bqm]] - hGLN liver isoform<br />		**[[4bqm]] - hGLN liver isoform<br />

	*GLN complex		*GLN complex

-	**[[3brm]] – BsGLN + norleucine <br />	+	**[[3brm]], [[2osu]] – BsGLN + norleucine derivative<br />
	**[[3czd]], [[3unw]] – hGLN + Glu<br />		**[[3czd]], [[3unw]] – hGLN + Glu<br />
	**[[3vp0]] – hGLN + Gln<br />		**[[3vp0]] – hGLN + Gln<br />
-	**[[3uo9]], [[3voz]], [[3vp2]], [[3vp3]], [[3vp4]], [[4o7d]] - hGLN + inhibitor<br />	+	**[[3uo9]], [[3voz]], [[3vp2]], [[3vp3]], [[3vp4]], [[4o7d]], [[5fi2]], [[5fi6]], [[5fi7]], [[5hl1]], [[5i94]], [[5jyo]], [[5jyp]] - hGLN + inhibitor<br />
	**[[3iha]], [[3ihb]], [[3age]] - MlGLN + Glu<br />		**[[3iha]], [[3ihb]], [[3age]] - MlGLN + Glu<br />
	**[[3ss5]] – mGLN + Glu<br />		**[[3ss5]] – mGLN + Glu<br />

Alexander Berchansky at 14:14, 13 March 2016

Alexander Berchansky — Sun, 13 Mar 2016 14:14:24 GMT

←Older revision		Revision as of 14:14, 13 March 2016
Line 8:		Line 8:

	== Structural highlights ==		== Structural highlights ==
-	The glutamate binding site is in the helical domain of GLN and <scene name='49/497107/Cv/2'>Tyr residue serves as a general acid in the catalysis</scene><ref>PMID:22049910</ref>. <scene name='49/497107/Cv/3'>Whole glutamate binding site</scene>.	+	The glutamate binding site is in the helical domain of GLN and <scene name='49/497107/Cv/4'>Tyr residue serves as a general acid in the catalysis</scene><ref>PMID:22049910</ref>. <scene name='49/497107/Cv/3'>Whole glutamate binding site</scene>.
	</StructureSection>		</StructureSection>
	==3D structures of glutaminase==		==3D structures of glutaminase==

Alexander Berchansky at 14:12, 13 March 2016

Alexander Berchansky — Sun, 13 Mar 2016 14:12:24 GMT

←Older revision		Revision as of 14:12, 13 March 2016
Line 8:		Line 8:

	== Structural highlights ==		== Structural highlights ==
-	The glutamate binding site is in the helical domain of GLN and <scene name='49/497107/Cv/2'>Tyr residue serves as a general acid in the catalysis</scene><ref>PMID:22049910</ref>.	+	The glutamate binding site is in the helical domain of GLN and <scene name='49/497107/Cv/2'>Tyr residue serves as a general acid in the catalysis</scene><ref>PMID:22049910</ref>. <scene name='49/497107/Cv/3'>Whole glutamate binding site</scene>.
	</StructureSection>		</StructureSection>
	==3D structures of glutaminase==		==3D structures of glutaminase==

Alexander Berchansky at 13:57, 13 March 2016

Alexander Berchansky — Sun, 13 Mar 2016 13:57:42 GMT

←Older revision		Revision as of 13:57, 13 March 2016
Line 8:		Line 8:

	== Structural highlights ==		== Structural highlights ==
-	The glutamate binding site is in the helical domain of GLN and Tyr residue serves as a general acid in the catalysis<ref>PMID:22049910</ref>.	+	The glutamate binding site is in the helical domain of GLN and <scene name='49/497107/Cv/2'>Tyr residue serves as a general acid in the catalysis</scene><ref>PMID:22049910</ref>.
	</StructureSection>		</StructureSection>
	==3D structures of glutaminase==		==3D structures of glutaminase==

Alexander Berchansky at 13:42, 13 March 2016

Alexander Berchansky — Sun, 13 Mar 2016 13:42:12 GMT

←Older revision		Revision as of 13:42, 13 March 2016
Line 1:		Line 1:
-	<StructureSection load='3unw' size='350' side='right' scene='' caption='Human glutaminase tetramer complex with glutamate [[3unw]]'>	+	<StructureSection load='3unw' size='350' side='right' scene='49/497107/Cv/1' caption='Human glutaminase tetramer complex with glutamate [[3unw]]'>

	== Function ==		== Function ==