Wayne Decatur at 17:10, 13 November 2018

Wayne Decatur — Tue, 13 Nov 2018 17:10:54 GMT

←Older revision		Revision as of 17:10, 13 November 2018
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	The conversion of uridine to pseudouridine causes conformational change of PUS<ref>PMID:14990747</ref>. <scene name='46/465439/Cv/3'>tRNA pseudouridine synthase binding with pseudouridine monophosphate</scene> ([[1ze2]]).		The conversion of uridine to pseudouridine causes conformational change of PUS<ref>PMID:14990747</ref>. <scene name='46/465439/Cv/3'>tRNA pseudouridine synthase binding with pseudouridine monophosphate</scene> ([[1ze2]]).
	</StructureSection>		</StructureSection>
-	== 3D Structures of pseudouridine synthase==	+	== 3D Structures of guide-independent pseudouridine synthase==

	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}		Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}

Wayne Decatur: separate out the two main types of pseudouridine synthase

Wayne Decatur — Tue, 13 Nov 2018 16:54:21 GMT

separate out the two main types of pseudouridine synthase

New page

<StructureSection load='1ze2' size='400' side='right' caption='tRNA pseudouridine synthase complex with RNA and pseudouridine monophosphate [[1ze2]]' scene='46/465439/Cv/1' pspeed='8'>
== Function ==
The alternative to using the RNA-guided mechanism for pseudouridine formation is a guide-independent mechanism acting through stand-alone enzymes. In eukaryotes, these enzymes are called Pus enzymes (Pseudouridine synthases), and they modify uridine by recognizing sequence and/or secondary structural elements of the RNA containing the target uridine.

'''pseudouridine synthase''' (PUS) catalyzes the conversion of uridine to tRNA pseudouridine in several types of RNA molecules<ref>PMID:14566049</ref>, <ref>PMID: 28045575</ref>. tRNAs are well-characterized as targets of these protein-only pseudouridine synthases, but recently have even been found to act on messenger RNAs in a variety of eukaryotic organisms, including humans<ref>PMID: 25192136</ref>, <ref>PMID: 25367125</ref>, <ref>PMID: 30414851</ref>. In eubacteria PUS are active in the ribosomal large subunit '''Ribosomal large subunit pseudouridine synthase''' and in the ribosomal small subunit '''Ribosomal small subunit pseudouridine synthase'''. 

Ribosomal large subunit pseudouridine synthases: 
*'''RluA''' is responsible for synthesis of pseudouridine from uracil in positions 746 in 23S ribosomal RNA and in the anticodon loop of tRNAPhe, tRNACys and tRNALeu<ref>PMID:17188032</ref>. 
*'''RluB''' is responsible for synthesis of pseudouridine from uracil in position 2605 in 23S ribosomal RNA<ref>PMID:24214967</ref>. 
*'''RluC''' is responsible for synthesis of pseudouridine from uracil in positions 955, 2504 and 2580 in 23S ribosomal RNA<ref>PMID:9660827</ref>. 
*'''RluD''' is responsible for synthesis of pseudouridine from uracil in positions 1911, 1915 and 1917 in 23S ribosomal RNA<ref>PMID:15928344</ref>. 
*'''RluE''' is responsible for synthesis of pseudouridine from uracil in position 2457 in 23S ribosomal RNA<ref>PMID:17320904</ref>. 
*'''RluF''' is responsible for synthesis of pseudouridine from uracil in position 2604 in 23S ribosomal RNA<ref>PMID:16712869</ref>. 

Ribosomal small subunit pseudouridine synthase '''RsuA''' is responsible for synthesis of pseudouridine from uracil in positions 516 in 16S ribosomal RNA<ref>PMID:11953756</ref>. 

== Structural highlights ==
The conversion of uridine to pseudouridine causes conformational change of PUS<ref>PMID:14990747</ref>. <scene name='46/465439/Cv/3'>tRNA pseudouridine synthase binding with pseudouridine monophosphate</scene> ([[1ze2]]).
</StructureSection>
== 3D Structures of pseudouridine synthase==

Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
{{#tree:id=OrganizedByTopic|openlevels=0|

*tRNA pseudouridine synthase

**[[1dj0]] – EcPUS I – ''Escherichia coli'' 
**[[4iqm]], [[4its]], [[4j37]], [[4nz6]] - hPUS Pus1 catalytic domain - human 
**[[4nz7]] - hPUS Pus1 catalytic domain (mutant) 
**[[1vs3]] – PUS TruA – ''Thermus thermophilus'' 
**[[1sgv]] – PUS TruB – ''Mycobacterium tuberculosis'' 
**[[1si7]], [[1szw]], [[1sb7]] - EcPUS TruD

*tRNA pseudouridine synthase complex with RNA

**[[1k8w]] – EcTruB + stem-loop RNA 
**[[1zl3]] - EcTruB (mutant) + stem-loop RNA 
**[[2nqp]], [[2nr0]], [[2nre]] – EcTruA + Leu-tRNA 
**[[1ze2]] - TmPUS B + RNA 
**[[2ab4]] - TmPUS B (mutant) + RNA 
**[[1r3e]], [[1r3f]] - EcTruB + RNA 

*Probable tRNA pseudouridine synthase

**[[3hjw]], [[3lwo]], [[3lwp]], [[3lwq]], [[3lwr]], [[3lwv]], [[3hax]] – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA – ''Pyrococcus furiosus'' 
**[[3hay]] – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA + small nuclear RNP protein 
**[[2hvy]] – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA + small nuclear RNP protein + small nuclear RNP Gar1-like protein 
**[[3mqk]] - PfPUS Cbf5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA 
**[[2rfk]] - PfPUS Cbf5 (mutant) + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA 
**[[2ey4]] - PfPUS Cbf5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein 
**[[3hjy]] - PfPUS Cbf5 + ribosome biogenesis protein NOP10 + RNA 
**[[2apo]] - PfPUS Cbf5 + ribosome biogenesis protein NOP10 - ''Methanocaldococcus jannaschii'' 

*Ribosomal large subunit pseudouridine synthase

**[[2i82]] - EcRluA catalytic domain + RNA 
**[[4lab]] - EcRluB catalytic domain 
**[[4lgt]] - EcRluB catalytic domain + RNA 
**[[1xpi]] - EcRluC catalytic domain 
**[[5vbb]] - hRluC-like 
**[[1prz]], [[1qyu]], [[1v9f]], [[1v9k]], [[2ist]] - EcRluD catalytic domain 
**[[2oml]], [[2olw]] - EcRluE catalytic domain 
**[[2gml]] - EcRluF catalytic domain 
**[[3dh3]] - EcRluF catalytic domain + RNA 

*Ribosomal small subunit pseudouridine synthase

**[[1vio]] - RsuA - ''Haemophilus influenzae'' 
**[[1ksk]] - EcRsuA 
**[[1ksl]] - EcRsuA + uracil 
**[[1ksv]] - EcRsuA + UMP 

}}
== References ==
<references/>
[[Category:Topic Page]]

Guide-independent Pseudouridine synthase - Revision history

Wayne Decatur at 17:10, 13 November 2018

Wayne Decatur: separate out the two main types of pseudouridine synthase