Image:AzI Final.pdb - Revision history

Alexander Berchansky at 08:39, 11 May 2008

Alexander Berchansky — Sun, 11 May 2008 08:39:18 GMT

←Older revision		Revision as of 08:39, 11 May 2008
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	=='''AzI_Final.pdb'''==		=='''AzI_Final.pdb'''==
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	<applet load='AzI_Final.pdb' size='500' frame='true' align='right' caption='sand' />		<applet load='AzI_Final.pdb' size='500' frame='true' align='right' caption='sand' />
		+
		+	Antizyme inhibitor (AzI) regulates cellular polyamine homeostasis by binding to the polyamine-induced protein, Antizyme (Az), with greater affinity than ODC. AzI is highly homologous to ornithine decarboxylase (ODC), but is not enzymatically active. In order to understand these specific characteristics of AzI and its differences from ODC, we determined the 3D structure of mouse AzI to 2.05Å resolution. Both AzI and ODC crystallize as a dimer. However, fewer interactions at the dimer interface, a smaller buried surface area, and lack of symmetry of the interactions between residues from the two monomers in the AzI structure suggest that this dimeric structure is non-physiological. In addition, the absence of residues and interactions required for PLP binding suggest that AzI does not bind PLP. Biochemical studies confirmed the lack of PLP binding and revealed that AzI exists as a monomer in solution while ODC is dimeric. Our findings that AzI exists as a monomer and its inability to bind PLP provide two independent explanations for its lack of enzymatic activity, and suggest the basis for its enhanced affinity towards Az.

Alexander Berchansky at 08:35, 11 May 2008

Alexander Berchansky — Sun, 11 May 2008 08:35:33 GMT

←Older revision		Revision as of 08:35, 11 May 2008
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-	=='''~~AzI Final~~.pdb'''==	+	=='''AzI_Final.pdb'''==
	This is a placeholder text to help you get started in		This is a placeholder text to help you get started in
	placing a Jmol applet on your page. At any time, click		placing a Jmol applet on your page. At any time, click
Line 7:		Line 7:
	and display another structure.		and display another structure.

-	<applet load='~~AzI Final~~.pdb' size='500' frame='true' align='right' caption='sand' />	+	<applet load='AzI_Final.pdb' size='500' frame='true' align='right' caption='sand' />

Alexander Berchansky at 08:34, 11 May 2008

Alexander Berchansky — Sun, 11 May 2008 08:34:54 GMT

←Older revision	Revision as of 08:34, 11 May 2008
Line 1:	Line 1:
	+	=='''AzI Final.pdb'''==
	+	This is a placeholder text to help you get started in
	+	placing a Jmol applet on your page. At any time, click
	+	"Show Preview" at the bottom of this page to see how it goes.

	+	Replace the PDB id after the STRUCTURE_ and after PDB= to load
	+	and display another structure.
	+
	+	<applet load='AzI Final.pdb' size='500' frame='true' align='right' caption='sand' />

Alexander Berchansky: uploaded a new version of "Image:AzI Final.pdb"

Alexander Berchansky — Sun, 11 May 2008 08:33:12 GMT

uploaded a new version of "Image:AzI Final.pdb"

←Older revision

Revision as of 08:33, 11 May 2008

Orly Dym at 12:14, 20 February 2008

Orly Dym — Wed, 20 Feb 2008 12:14:02 GMT

New page