Inositol Monophosphatase - Revision history

Michal Harel at 07:16, 29 August 2019

Michal Harel — Thu, 29 Aug 2019 07:16:35 GMT

←Older revision		Revision as of 07:16, 29 August 2019
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	</StructureSection>		</StructureSection>
-	=3D Structures of Inositol Monophosphatase=
-	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
-	{{#tree:id=OrganizedByTopic\|openlevels=0\|

-	*Inositol monophosphatase
-
-	**[[2ddk]], [[2czk]], [[2fvz]] – hIMP 2 – human<br />
-	**[[4as4]] – hIMP 1<br />
-	**[[1imf]] – hIMP<br />
-	**[[2bji]] – IMP 1 - bovine<br />
-	**[[4as5]] – IMP 1 - mouse<br />
-	**[[2qfl]], [[6ib7]] – IMP – ''Escherichia coli''<br />
-	**[[2p3n]], [[2p3v]] – IMP – ''Thermotoga maritima''<br />
-	**[[2pcr]] – IMP – ''Aquifex aeolicus''<br />
-	**[[2q74]], [[5djf]] – MtIMP – ''Mycobacterium tuberculosis''<br />
-	**[[3qmf]] – SaIMP – ''Staphylococcus aureus''<br />
-	**[[3t0j]] – SaIMP 2<br />
-	**[[4n81]] - IMP - ''Zymomonas mobilis''<br />
-	**[[5eqa]] – MtIMP – ''Medicago truncatula''<br />
-	**[[5zhh]] - IMP - ''Anabaena''<br />
-
-	*Inositol monophosphatase binary complex
-
-	**[[2hhm]] – hIMP + sulfate<br />
-	**[[2czh]] – hIMP 2 + phosphate<br />
-	**[[1ima]], [[1imb]] - hIMP + inositol-1-phosphate<br />
-	**[[1ime]] – hIMP + Ca<br />
-	**[[6giu]] – hIMP + lithium mimetic<br />
-	**[[6gj0]] – hIMP + Mn<br />
-	**[[1g0h]], [[1g0i]] - MjIMP + inositol-1-phosphate<br />
-	**[[3ryd]] – SaIMP + Ca<br />
-	**[[5i3s]] – SaIMP 2 + phosphate <br />
-	**[[5eq7]], [[5t3j]] – MtIMP + phosphate <br />
-	**[[5eq8]], [[5eq9]] – MtIMP + histidinol<br />
-	**[[6ib8]] – EcIMP + NUSA<br />
-
-	*Inositol monophosphatase ternary complex
-
-	**[[2czi]] - hIMP 2 + phosphate + Ca<br />
-	**[[1imd]] - hIMP + phosphate + Mn<br />
-	**[1awb]] - hIMP + inositol-1-phosphate + Ca<br />
-	**[[1imc]] – hIMP + Mn + Cl<br />
-	**[[1dk4]] – MjIMP + phosphate + Zn – ''Methanocaldococcus jannaschii''<br />
-	**[[5f24]] - SaIMP + inositol-1-phosphate + Ca<br />
-	**[[4g61]], [[4i3e]], [[4i3y]], [[4i40]], [[4ptk]] - SaIMP 1 + phosphate + Mg<br />
-	**[[5j16]] - SaIMP 2 + phosphate + Ca<br />
-	**[[5eyg]], [[5eyh]] - SaIMP + NADP + Ca<br />
-	**[[5eyh]], [[5dw8]] - SaIMP + AMP + Ca<br />
-	**[[5djg]] - MtIMP + A3P + Mg <br />
-	**[[5djj]] - MtIMP + AMP + Mg <br />
-	**[[5djk]] - MtIMP + Ca + phosphate<br />
-	**[[5djh]], [[5dji]] - MtIMP + AMP + Mg + phosphate<br />
-
-	*Fructose-1,6-bisphosphatase/inositol-1- monophosphatase
-
-	**[[1lbw]] – AfFBPase/IMPase – Archaeoglobus fulgidus<br />
-	**[[6b65]] – AfFBPase/IMPase (mutant) <br />
-	**[[6b61]], [[6b63]] – AfFBPase/IMPase + Asp <br />
-	**[[6b64]] – AfFBPase/IMPase (mutant) + Asp <br />
-	**[[6b5z]] – AfFBPase/IMPase + inositol + Asp <br />
-	**[[6b60]], [[6b62]] – AfFBPase/IMPase + Glu <br />
-	**[[6b66]] – AfFBPase/IMPase (mutant) + Glu <br />
-	**[[1lbx]] – AfFBPase/IMPase + inositol-1-phosphate + Ca <br />
-	**[[1lbz]] – AfFBPase/IMPase + fructose-1,6-bisphosphate + Ca <br />
-	**[[1lby]] – AfFBPase/IMPase + fructose-6-phosphate + PO4 + Mn <br />
-
-	}}
	=References=		=References=
	<references/>		<references/>

	[[Category:Topic Page]]		[[Category:Topic Page]]

Michal Harel at 07:13, 29 August 2019

Michal Harel — Thu, 29 Aug 2019 07:13:25 GMT

←Older revision		Revision as of 07:13, 29 August 2019
Line 23:		Line 23:
	It is generally well received that [http://en.wikipedia.org/wiki/Hyperthermophile\| hyperthermophiles] are near the oldest phyletic group, with a very slow rate of evolution<ref name="rasmol"/>. This is due to the rather unforgiving environment toward mutations of these microbes. Given that the MJ0109 protein lacks substrate specificity and regulatory sites, and possess activity of two separate pathways in Eukaryotes; it is a reasonable assumption that this enzyme is an evolutionary snap shot of an original phosphatase approximation<ref name="rasmol"/>.		It is generally well received that [http://en.wikipedia.org/wiki/Hyperthermophile\| hyperthermophiles] are near the oldest phyletic group, with a very slow rate of evolution<ref name="rasmol"/>. This is due to the rather unforgiving environment toward mutations of these microbes. Given that the MJ0109 protein lacks substrate specificity and regulatory sites, and possess activity of two separate pathways in Eukaryotes; it is a reasonable assumption that this enzyme is an evolutionary snap shot of an original phosphatase approximation<ref name="rasmol"/>.

-	==3D Structures of Inositol Monophosphatase==	+	=3D Structures of Inositol Monophosphatase=
	[[Inositol monophosphatase 3D structures]]		[[Inositol monophosphatase 3D structures]]

Michal Harel at 07:12, 29 August 2019

Michal Harel — Thu, 29 Aug 2019 07:12:44 GMT

←Older revision		Revision as of 07:12, 29 August 2019
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	===FBPase===		===FBPase===
	Under normal conditions the MJ0109 gene product preferentially metabolizes the second last major step in [http://en.wikipedia.org/wiki/Gluconeogenesis\| gluconeogenesis], fructose-1,6-bisphosphate substrate to fructose-6-phosphate. Relative to its IMPase activity, the FBPase activity of MJ0109 is 1.63 times as active<ref name="rasmol"/>. This particular function was not immediately identified by genomic sequence alignment and was later determined using structual and kinetic analysis<ref name="rasmol"/>. FBPase K<sub>m</sub> and K<sub>cat</sub> at 85°C; 38±9μM and 7.0±0.4/s, respectively<ref name="rasmol"/>.		Under normal conditions the MJ0109 gene product preferentially metabolizes the second last major step in [http://en.wikipedia.org/wiki/Gluconeogenesis\| gluconeogenesis], fructose-1,6-bisphosphate substrate to fructose-6-phosphate. Relative to its IMPase activity, the FBPase activity of MJ0109 is 1.63 times as active<ref name="rasmol"/>. This particular function was not immediately identified by genomic sequence alignment and was later determined using structual and kinetic analysis<ref name="rasmol"/>. FBPase K<sub>m</sub> and K<sub>cat</sub> at 85°C; 38±9μM and 7.0±0.4/s, respectively<ref name="rasmol"/>.
		+	===FBPase/IMPase===
		+	An unusual phosphatase found in several hyperthermophilic organisms which has dual activity toward inositol monophosphate and fructose 1,6-bisphosphate<ref>PMID:11940584</ref>.
	===Inhibitors===		===Inhibitors===
	As the dual activity indicates, the substrate specificity of this enzyme is particularly low. Proposed evolutionary cousins of this enzyme are regulated by [http://en.wikipedia.org/wiki/Adenosine_monophosphate\| Adenosine monophosphate] and [http://en.wikipedia.org/wiki/Fructose_2,6-bisphosphate\| Fructose 2,6-bisphosphate]. However, there are no such allosteric regulatory binding sites on the MJ0109 protein. Additionally, ''M. jannaschii'' IMPase has a [http://en.wikipedia.org/wiki/IC50\| IC50] for Li+ of >150mM, compared to the Li+ IC50 of human IMPase <1mM<ref name="rasmol"/>.		As the dual activity indicates, the substrate specificity of this enzyme is particularly low. Proposed evolutionary cousins of this enzyme are regulated by [http://en.wikipedia.org/wiki/Adenosine_monophosphate\| Adenosine monophosphate] and [http://en.wikipedia.org/wiki/Fructose_2,6-bisphosphate\| Fructose 2,6-bisphosphate]. However, there are no such allosteric regulatory binding sites on the MJ0109 protein. Additionally, ''M. jannaschii'' IMPase has a [http://en.wikipedia.org/wiki/IC50\| IC50] for Li+ of >150mM, compared to the Li+ IC50 of human IMPase <1mM<ref name="rasmol"/>.
	=Evolution=		=Evolution=
	It is generally well received that [http://en.wikipedia.org/wiki/Hyperthermophile\| hyperthermophiles] are near the oldest phyletic group, with a very slow rate of evolution<ref name="rasmol"/>. This is due to the rather unforgiving environment toward mutations of these microbes. Given that the MJ0109 protein lacks substrate specificity and regulatory sites, and possess activity of two separate pathways in Eukaryotes; it is a reasonable assumption that this enzyme is an evolutionary snap shot of an original phosphatase approximation<ref name="rasmol"/>.		It is generally well received that [http://en.wikipedia.org/wiki/Hyperthermophile\| hyperthermophiles] are near the oldest phyletic group, with a very slow rate of evolution<ref name="rasmol"/>. This is due to the rather unforgiving environment toward mutations of these microbes. Given that the MJ0109 protein lacks substrate specificity and regulatory sites, and possess activity of two separate pathways in Eukaryotes; it is a reasonable assumption that this enzyme is an evolutionary snap shot of an original phosphatase approximation<ref name="rasmol"/>.
		+
		+	==3D Structures of Inositol Monophosphatase==
		+	[[Inositol monophosphatase 3D structures]]
		+
	</StructureSection>		</StructureSection>
	=3D Structures of Inositol Monophosphatase=		=3D Structures of Inositol Monophosphatase=
Line 27:		Line 33:
	*Inositol monophosphatase		*Inositol monophosphatase

-	**[[2bji]] – IMP 1 - bovine<br />
	**[[2ddk]], [[2czk]], [[2fvz]] – hIMP 2 – human<br />		**[[2ddk]], [[2czk]], [[2fvz]] – hIMP 2 – human<br />
	**[[4as4]] – hIMP 1<br />		**[[4as4]] – hIMP 1<br />
	**[[1imf]] – hIMP<br />		**[[1imf]] – hIMP<br />
		+	**[[2bji]] – IMP 1 - bovine<br />
	**[[4as5]] – IMP 1 - mouse<br />		**[[4as5]] – IMP 1 - mouse<br />
-	**[[2qfl]] – IMP – ''Escherichia coli''<br />	+	**[[2qfl]], [[6ib7]] – IMP – ''Escherichia coli''<br />
	**[[2p3n]], [[2p3v]] – IMP – ''Thermotoga maritima''<br />		**[[2p3n]], [[2p3v]] – IMP – ''Thermotoga maritima''<br />
	**[[2pcr]] – IMP – ''Aquifex aeolicus''<br />		**[[2pcr]] – IMP – ''Aquifex aeolicus''<br />
Line 40:		Line 46:
	**[[4n81]] - IMP - ''Zymomonas mobilis''<br />		**[[4n81]] - IMP - ''Zymomonas mobilis''<br />
	**[[5eqa]] – MtIMP – ''Medicago truncatula''<br />		**[[5eqa]] – MtIMP – ''Medicago truncatula''<br />
		+	**[[5zhh]] - IMP - ''Anabaena''<br />

	*Inositol monophosphatase binary complex		*Inositol monophosphatase binary complex
Line 47:		Line 54:
	**[[1ima]], [[1imb]] - hIMP + inositol-1-phosphate<br />		**[[1ima]], [[1imb]] - hIMP + inositol-1-phosphate<br />
	**[[1ime]] – hIMP + Ca<br />		**[[1ime]] – hIMP + Ca<br />
		+	**[[6giu]] – hIMP + lithium mimetic<br />
		+	**[[6gj0]] – hIMP + Mn<br />
	**[[1g0h]], [[1g0i]] - MjIMP + inositol-1-phosphate<br />		**[[1g0h]], [[1g0i]] - MjIMP + inositol-1-phosphate<br />
	**[[3ryd]] – SaIMP + Ca<br />		**[[3ryd]] – SaIMP + Ca<br />
Line 52:		Line 61:
	**[[5eq7]], [[5t3j]] – MtIMP + phosphate <br />		**[[5eq7]], [[5t3j]] – MtIMP + phosphate <br />
	**[[5eq8]], [[5eq9]] – MtIMP + histidinol<br />		**[[5eq8]], [[5eq9]] – MtIMP + histidinol<br />
		+	**[[6ib8]] – EcIMP + NUSA<br />

	*Inositol monophosphatase ternary complex		*Inositol monophosphatase ternary complex
Line 69:		Line 79:
	**[[5djk]] - MtIMP + Ca + phosphate<br />		**[[5djk]] - MtIMP + Ca + phosphate<br />
	**[[5djh]], [[5dji]] - MtIMP + AMP + Mg + phosphate<br />		**[[5djh]], [[5dji]] - MtIMP + AMP + Mg + phosphate<br />
		+
		+	*Fructose-1,6-bisphosphatase/inositol-1- monophosphatase
		+
		+	**[[1lbw]] – AfFBPase/IMPase – Archaeoglobus fulgidus<br />
		+	**[[6b65]] – AfFBPase/IMPase (mutant) <br />
		+	**[[6b61]], [[6b63]] – AfFBPase/IMPase + Asp <br />
		+	**[[6b64]] – AfFBPase/IMPase (mutant) + Asp <br />
		+	**[[6b5z]] – AfFBPase/IMPase + inositol + Asp <br />
		+	**[[6b60]], [[6b62]] – AfFBPase/IMPase + Glu <br />
		+	**[[6b66]] – AfFBPase/IMPase (mutant) + Glu <br />
		+	**[[1lbx]] – AfFBPase/IMPase + inositol-1-phosphate + Ca <br />
		+	**[[1lbz]] – AfFBPase/IMPase + fructose-1,6-bisphosphate + Ca <br />
		+	**[[1lby]] – AfFBPase/IMPase + fructose-6-phosphate + PO4 + Mn <br />
		+
	}}		}}
	=References=		=References=

Michal Harel at 06:11, 22 July 2018

Michal Harel — Sun, 22 Jul 2018 06:11:56 GMT

←Older revision		Revision as of 06:11, 22 July 2018
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	**[[2p3n]], [[2p3v]] – IMP – ''Thermotoga maritima''<br />		**[[2p3n]], [[2p3v]] – IMP – ''Thermotoga maritima''<br />
	**[[2pcr]] – IMP – ''Aquifex aeolicus''<br />		**[[2pcr]] – IMP – ''Aquifex aeolicus''<br />
-	**[[2q74]] – ~~IMP~~ – ''Mycobacterium tuberculosis''<br />	+	**[[2q74]], [[5djf]] – MtIMP – ''Mycobacterium tuberculosis''<br />
	**[[3qmf]] – SaIMP – ''Staphylococcus aureus''<br />		**[[3qmf]] – SaIMP – ''Staphylococcus aureus''<br />
	**[[3t0j]] – SaIMP 2<br />		**[[3t0j]] – SaIMP 2<br />

Michal Harel at 10:08, 19 July 2018

Michal Harel — Thu, 19 Jul 2018 10:08:12 GMT

←Older revision		Revision as of 10:08, 19 July 2018
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	**[[5j16]] - SaIMP 2 + phosphate + Ca<br />		**[[5j16]] - SaIMP 2 + phosphate + Ca<br />
	**[[5eyg]], [[5eyh]] - SaIMP + NADP + Ca<br />		**[[5eyg]], [[5eyh]] - SaIMP + NADP + Ca<br />
-	**[[5eyh]] - SaIMP + AMP + Ca<br />	+	**[[5eyh]], [[5dw8]] - SaIMP + AMP + Ca<br />
		+	**[[5djg]] - MtIMP + A3P + Mg <br />
		+	**[[5djj]] - MtIMP + AMP + Mg <br />
		+	**[[5djk]] - MtIMP + Ca + phosphate<br />
		+	**[[5djh]], [[5dji]] - MtIMP + AMP + Mg + phosphate<br />
	}}		}}
	=References=		=References=

Michal Harel at 10:11, 18 June 2017

Michal Harel — Sun, 18 Jun 2017 10:11:22 GMT

←Older revision		Revision as of 10:11, 18 June 2017
Line 1:		Line 1:
-
	<StructureSection load='1dk4' size='340' side='right' caption='Inositol monophosphatase dimer complex with Zn+2 (grey) and phosphate ions, [[1dk4]]' scene='Sandbox_Reserved_326/Begininggood/1'>		<StructureSection load='1dk4' size='340' side='right' caption='Inositol monophosphatase dimer complex with Zn+2 (grey) and phosphate ions, [[1dk4]]' scene='Sandbox_Reserved_326/Begininggood/1'>

Line 40:		Line 39:
	**[[3t0j]] – SaIMP 2<br />		**[[3t0j]] – SaIMP 2<br />
	**[[4n81]] - IMP - ''Zymomonas mobilis''<br />		**[[4n81]] - IMP - ''Zymomonas mobilis''<br />
		+	**[[5eqa]] – MtIMP – ''Medicago truncatula''<br />

	*Inositol monophosphatase binary complex		*Inositol monophosphatase binary complex
Line 49:		Line 49:
	**[[1g0h]], [[1g0i]] - MjIMP + inositol-1-phosphate<br />		**[[1g0h]], [[1g0i]] - MjIMP + inositol-1-phosphate<br />
	**[[3ryd]] – SaIMP + Ca<br />		**[[3ryd]] – SaIMP + Ca<br />
		+	**[[5i3s]] – SaIMP 2 + phosphate <br />
		+	**[[5eq7]], [[5t3j]] – MtIMP + phosphate <br />
		+	**[[5eq8]], [[5eq9]] – MtIMP + histidinol<br />

	*Inositol monophosphatase ternary complex		*Inositol monophosphatase ternary complex
Line 59:		Line 62:
	**[[5f24]] - SaIMP + inositol-1-phosphate + Ca<br />		**[[5f24]] - SaIMP + inositol-1-phosphate + Ca<br />
	**[[4g61]], [[4i3e]], [[4i3y]], [[4i40]], [[4ptk]] - SaIMP 1 + phosphate + Mg<br />		**[[4g61]], [[4i3e]], [[4i3y]], [[4i40]], [[4ptk]] - SaIMP 1 + phosphate + Mg<br />
		+	**[[5j16]] - SaIMP 2 + phosphate + Ca<br />
	**[[5eyg]], [[5eyh]] - SaIMP + NADP + Ca<br />		**[[5eyg]], [[5eyh]] - SaIMP + NADP + Ca<br />
-		+	**[[5eyh]] - SaIMP + AMP + Ca<br />
	}}		}}
	=References=		=References=

Michal Harel at 09:42, 18 June 2017

Michal Harel — Sun, 18 Jun 2017 09:42:44 GMT

←Older revision		Revision as of 09:42, 18 June 2017
Line 1:		Line 1:
-	~~{{STRUCTURE_1dk4\| PDB~~=1dk4 ~~\| SIZE~~=~~350\| SCENE~~=~~Sandbox_Reserved_326/Begininggood/1\|~~right~~\| CAPTION~~= Inositol monophosphatase dimer complex with Zn+2 (grey) and phosphate ions, [[1dk4]] }}	+
		+	<StructureSection load='1dk4' size='340' side='right' caption='Inositol monophosphatase dimer complex with Zn+2 (grey) and phosphate ions, [[1dk4]]' scene='Sandbox_Reserved_326/Begininggood/1'>

	=Introduction=		=Introduction=
Line 20:		Line 21:
	=Evolution=		=Evolution=
	It is generally well received that [http://en.wikipedia.org/wiki/Hyperthermophile\| hyperthermophiles] are near the oldest phyletic group, with a very slow rate of evolution<ref name="rasmol"/>. This is due to the rather unforgiving environment toward mutations of these microbes. Given that the MJ0109 protein lacks substrate specificity and regulatory sites, and possess activity of two separate pathways in Eukaryotes; it is a reasonable assumption that this enzyme is an evolutionary snap shot of an original phosphatase approximation<ref name="rasmol"/>.		It is generally well received that [http://en.wikipedia.org/wiki/Hyperthermophile\| hyperthermophiles] are near the oldest phyletic group, with a very slow rate of evolution<ref name="rasmol"/>. This is due to the rather unforgiving environment toward mutations of these microbes. Given that the MJ0109 protein lacks substrate specificity and regulatory sites, and possess activity of two separate pathways in Eukaryotes; it is a reasonable assumption that this enzyme is an evolutionary snap shot of an original phosphatase approximation<ref name="rasmol"/>.
-		+	</StructureSection>
	=3D Structures of Inositol Monophosphatase=		=3D Structures of Inositol Monophosphatase=
	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}		Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}

Michal Harel at 10:09, 17 November 2016

Michal Harel — Thu, 17 Nov 2016 10:09:00 GMT

←Older revision		Revision as of 10:09, 17 November 2016
Line 1:		Line 1:
-	{{STRUCTURE_1dk4\| PDB=1dk4 \| SIZE=~~400~~\| SCENE=Sandbox_Reserved_326/Begininggood/1\|right\| CAPTION= Inositol monophosphatase dimer complex with Zn+2 (grey) and phosphate ions, [[1dk4]] }}	+	{{STRUCTURE_1dk4\| PDB=1dk4 \| SIZE=350\| SCENE=Sandbox_Reserved_326/Begininggood/1\|right\| CAPTION= Inositol monophosphatase dimer complex with Zn+2 (grey) and phosphate ions, [[1dk4]] }}

	=Introduction=		=Introduction=
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	**[[1imc]] – hIMP + Mn + Cl<br />		**[[1imc]] – hIMP + Mn + Cl<br />
	**[[1dk4]] – MjIMP + phosphate + Zn – ''Methanocaldococcus jannaschii''<br />		**[[1dk4]] – MjIMP + phosphate + Zn – ''Methanocaldococcus jannaschii''<br />
-	**[[~~4g60~~]] - SaIMP + inositol-1-phosphate + Ca<br />	+	**[[5f24]] - SaIMP + inositol-1-phosphate + Ca<br />
	**[[4g61]], [[4i3e]], [[4i3y]], [[4i40]], [[4ptk]] - SaIMP 1 + phosphate + Mg<br />		**[[4g61]], [[4i3e]], [[4i3y]], [[4i40]], [[4ptk]] - SaIMP 1 + phosphate + Mg<br />
-	**[[~~4g62~~]], [[~~4g64~~]] - SaIMP + NADP + Ca<br />	+	**[[5eyg]], [[5eyh]] - SaIMP + NADP + Ca<br />

	}}		}}

Michal Harel at 11:01, 28 March 2016

Michal Harel — Mon, 28 Mar 2016 11:01:21 GMT

←Older revision		Revision as of 11:01, 28 March 2016
Line 1:		Line 1:
	{{STRUCTURE_1dk4\| PDB=1dk4 \| SIZE=400\| SCENE=Sandbox_Reserved_326/Begininggood/1\|right\| CAPTION= Inositol monophosphatase dimer complex with Zn+2 (grey) and phosphate ions, [[1dk4]] }}		{{STRUCTURE_1dk4\| PDB=1dk4 \| SIZE=400\| SCENE=Sandbox_Reserved_326/Begininggood/1\|right\| CAPTION= Inositol monophosphatase dimer complex with Zn+2 (grey) and phosphate ions, [[1dk4]] }}

-		+	=Introduction=
	<scene name='Sandbox_Reserved_326/Begininggood/1'>Inositol Monophosphatase</scene> ([[1dk4]]) is a homodimeric phosphatase. This protein structure is derived from the the methanogen ''Methanococcus jannaschii'' gene MJ0109. Curiously this protein shows both Inositol Monophosphatase (IMPase) activity as well as Fructose-1,6-Bisphosphatase acivity (FBPase)<ref name="rasmol">PMID:11062561</ref>. This protein has a homolog in other archaeabacteria, such as [[1lbv\|''Archaeoglobus fulgidus'']]<ref name="ref2">PMID:11940584</ref>. This Archaeal phosphatase is thought to be a precursor of many phophatases of higher organisms in Eukaryotes and Bacteria<ref name="rasmol"/>.		<scene name='Sandbox_Reserved_326/Begininggood/1'>Inositol Monophosphatase</scene> ([[1dk4]]) is a homodimeric phosphatase. This protein structure is derived from the the methanogen ''Methanococcus jannaschii'' gene MJ0109. Curiously this protein shows both Inositol Monophosphatase (IMPase) activity as well as Fructose-1,6-Bisphosphatase acivity (FBPase)<ref name="rasmol">PMID:11062561</ref>. This protein has a homolog in other archaeabacteria, such as [[1lbv\|''Archaeoglobus fulgidus'']]<ref name="ref2">PMID:11940584</ref>. This Archaeal phosphatase is thought to be a precursor of many phophatases of higher organisms in Eukaryotes and Bacteria<ref name="rasmol"/>.
-	~~__TOC__~~	+
	=''Methanococcus jannaschii''=		=''Methanococcus jannaschii''=
	[http://en.wikipedia.org/wiki/Methanocaldococcus\|''M. jannaschii''] are thermophilc methane producing archaeabacteria that were discovered in 1983 by the manned submersible ALVIN[http://www.springerlink.com/content/u8774k126821777x/]. They were sampled from the base of a deep sea hydrothermal vent in 2600m of water, local tempurature was 85°C. The complete 1.66 mega base pairs of its genome has been sequenced, about 1738 genes were identified. Most of them were determined to be homologous to eukaryotic proteins<ref>PMID: 8688087</ref>.		[http://en.wikipedia.org/wiki/Methanocaldococcus\|''M. jannaschii''] are thermophilc methane producing archaeabacteria that were discovered in 1983 by the manned submersible ALVIN[http://www.springerlink.com/content/u8774k126821777x/]. They were sampled from the base of a deep sea hydrothermal vent in 2600m of water, local tempurature was 85°C. The complete 1.66 mega base pairs of its genome has been sequenced, about 1738 genes were identified. Most of them were determined to be homologous to eukaryotic proteins<ref>PMID: 8688087</ref>.
Line 10:		Line 10:

	One subunit of MJ0109 is composed of <scene name='Sandbox_Reserved_326/2nd_structure_unbcolour/3'>5 layers</scene> alternating <font color='green'>α helix</font>, <font color='gold'>β sheet</font>, to <font color='green'>α helix</font> from the N to C terminus.		One subunit of MJ0109 is composed of <scene name='Sandbox_Reserved_326/2nd_structure_unbcolour/3'>5 layers</scene> alternating <font color='green'>α helix</font>, <font color='gold'>β sheet</font>, to <font color='green'>α helix</font> from the N to C terminus.
-	The active site is mostly conserved and is similar in sequence to [[1awb\|Human IMPase]]. However, its overall tertiary structure is similar to a monomer of the ''Sus scrofa domesticus'', [[1cnq\|FBPase]].	+	The active site is mostly conserved and is similar in sequence to [[1awb\|Human IMPase]]. However, its overall tertiary structure is similar to a monomer of the ''Sus scrofa domesticus'', [[1cnq\|FBPase]] ([[Fructose-1,6-bisphosphatase]]).
	=Function=		=Function=
	===IMPase===		===IMPase===

Michal Harel at 10:31, 26 April 2015

Michal Harel — Sun, 26 Apr 2015 10:31:35 GMT

←Older revision		Revision as of 10:31, 26 April 2015
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	**[[2pcr]] – IMP – ''Aquifex aeolicus''<br />		**[[2pcr]] – IMP – ''Aquifex aeolicus''<br />
	**[[2q74]] – IMP – ''Mycobacterium tuberculosis''<br />		**[[2q74]] – IMP – ''Mycobacterium tuberculosis''<br />
		+	**[[3qmf]] – SaIMP – ''Staphylococcus aureus''<br />
		+	**[[3t0j]] – SaIMP 2<br />
		+	**[[4n81]] - IMP - ''Zymomonas mobilis''<br />

	*Inositol monophosphatase binary complex		*Inositol monophosphatase binary complex
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	**[[1ime]] – hIMP + Ca<br />		**[[1ime]] – hIMP + Ca<br />
	**[[1g0h]], [[1g0i]] - MjIMP + inositol-1-phosphate<br />		**[[1g0h]], [[1g0i]] - MjIMP + inositol-1-phosphate<br />
		+	**[[3ryd]] – SaIMP + Ca<br />

	*Inositol monophosphatase ternary complex		*Inositol monophosphatase ternary complex
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	**[[1imc]] – hIMP + Mn + Cl<br />		**[[1imc]] – hIMP + Mn + Cl<br />
	**[[1dk4]] – MjIMP + phosphate + Zn – ''Methanocaldococcus jannaschii''<br />		**[[1dk4]] – MjIMP + phosphate + Zn – ''Methanocaldococcus jannaschii''<br />
		+	**[[4g60]] - SaIMP + inositol-1-phosphate + Ca<br />
		+	**[[4g61]], [[4i3e]], [[4i3y]], [[4i40]], [[4ptk]] - SaIMP 1 + phosphate + Mg<br />
		+	**[[4g62]], [[4g64]] - SaIMP + NADP + Ca<br />
		+
	}}		}}
	=References=		=References=