Integrator complex - Revision history

Alexander Berchansky at 13:44, 1 May 2024

Alexander Berchansky — Wed, 01 May 2024 13:44:54 GMT

←Older revision		Revision as of 13:44, 1 May 2024
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	*<scene name='10/1043660/Cv/13'>For simplicity of viewing, only few interactions involving Leu773, Gln774, Met782, Phe806, Tyr809, Leu845, Leu846, Gln871, and their dyad symmetry mates are shown at the dimer interface of Int3</scene>.		*<scene name='10/1043660/Cv/13'>For simplicity of viewing, only few interactions involving Leu773, Gln774, Met782, Phe806, Tyr809, Leu845, Leu846, Gln871, and their dyad symmetry mates are shown at the dimer interface of Int3</scene>.
	*<scene name='10/1043660/Cv/11'>Whole Int3/Int6 interface</scene>.		*<scene name='10/1043660/Cv/11'>Whole Int3/Int6 interface</scene>.
-	*<scene name='10/1043660/Cv/14'>The Int3/Int6 interface is composed of central hydrophobic residues, Pro823, Phe858 from Int6, and Phe806 from Int3</scene>	+	*<scene name='10/1043660/Cv/14'>The Int3/Int6 interface is composed of central hydrophobic residues, Pro823, Phe858 from Int6, and Phe806 from Int3</scene>. These hydrophobic interactions are further strengthened <scene name='10/1043660/Cv/17'> by salt bridges and hydrogen bonds</scene>.
-		+
	==Integrator complex 3D structures==		==Integrator complex 3D structures==

Alexander Berchansky at 13:20, 1 May 2024

Alexander Berchansky — Wed, 01 May 2024 13:20:36 GMT

←Older revision		Revision as of 13:20, 1 May 2024
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	*<scene name='10/1043660/Cv/8'>Whole dimer interface of Int3</scene>.		*<scene name='10/1043660/Cv/8'>Whole dimer interface of Int3</scene>.
	*<scene name='10/1043660/Cv/13'>For simplicity of viewing, only few interactions involving Leu773, Gln774, Met782, Phe806, Tyr809, Leu845, Leu846, Gln871, and their dyad symmetry mates are shown at the dimer interface of Int3</scene>.		*<scene name='10/1043660/Cv/13'>For simplicity of viewing, only few interactions involving Leu773, Gln774, Met782, Phe806, Tyr809, Leu845, Leu846, Gln871, and their dyad symmetry mates are shown at the dimer interface of Int3</scene>.
-	*<scene name='10/1043660/Cv/11'>~~The~~ Int3/Int6 interface</scene>.	+	*<scene name='10/1043660/Cv/11'>Whole Int3/Int6 interface</scene>.
		+	*<scene name='10/1043660/Cv/14'>The Int3/Int6 interface is composed of central hydrophobic residues, Pro823, Phe858 from Int6, and Phe806 from Int3</scene>

	==Integrator complex 3D structures==		==Integrator complex 3D structures==

Alexander Berchansky at 13:05, 1 May 2024

Alexander Berchansky — Wed, 01 May 2024 13:05:39 GMT

←Older revision		Revision as of 13:05, 1 May 2024
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	*Two Int3 protomers form a <scene name='10/1043660/Cv/4'>symmetrical dimer</scene> wherein two protomers are related by a non-crystallographic two-fold axis.		*Two Int3 protomers form a <scene name='10/1043660/Cv/4'>symmetrical dimer</scene> wherein two protomers are related by a non-crystallographic two-fold axis.
	*<scene name='10/1043660/Cv/8'>Whole dimer interface of Int3</scene>.		*<scene name='10/1043660/Cv/8'>Whole dimer interface of Int3</scene>.
-		+	*<scene name='10/1043660/Cv/13'>For simplicity of viewing, only few interactions involving Leu773, Gln774, Met782, Phe806, Tyr809, Leu845, Leu846, Gln871, and their dyad symmetry mates are shown at the dimer interface of Int3</scene>.
	*<scene name='10/1043660/Cv/11'>The Int3/Int6 interface</scene>.		*<scene name='10/1043660/Cv/11'>The Int3/Int6 interface</scene>.

Alexander Berchansky at 12:56, 1 May 2024

Alexander Berchansky — Wed, 01 May 2024 12:56:16 GMT

←Older revision		Revision as of 12:56, 1 May 2024
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	*Two Int3 protomers form a <scene name='10/1043660/Cv/4'>symmetrical dimer</scene> wherein two protomers are related by a non-crystallographic two-fold axis.		*Two Int3 protomers form a <scene name='10/1043660/Cv/4'>symmetrical dimer</scene> wherein two protomers are related by a non-crystallographic two-fold axis.
-	*<scene name='10/1043660/Cv/8'>~~The~~ dimer interface of Int3</scene>.	+	*<scene name='10/1043660/Cv/8'>Whole dimer interface of Int3</scene>.
		+
	*<scene name='10/1043660/Cv/11'>The Int3/Int6 interface</scene>.		*<scene name='10/1043660/Cv/11'>The Int3/Int6 interface</scene>.

Alexander Berchansky at 13:50, 30 April 2024

Alexander Berchansky — Tue, 30 Apr 2024 13:50:34 GMT

←Older revision		Revision as of 13:50, 30 April 2024
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	*Two Int3 protomers form a <scene name='10/1043660/Cv/4'>symmetrical dimer</scene> wherein two protomers are related by a non-crystallographic two-fold axis.		*Two Int3 protomers form a <scene name='10/1043660/Cv/4'>symmetrical dimer</scene> wherein two protomers are related by a non-crystallographic two-fold axis.
	*<scene name='10/1043660/Cv/8'>The dimer interface of Int3</scene>.		*<scene name='10/1043660/Cv/8'>The dimer interface of Int3</scene>.
-	*<scene name='10/1043660/Cv/10'>The Int3/Int6 interface</scene>.	+	*<scene name='10/1043660/Cv/11'>The Int3/Int6 interface</scene>.

	==Integrator complex 3D structures==		==Integrator complex 3D structures==

Alexander Berchansky at 13:45, 30 April 2024

Alexander Berchansky — Tue, 30 Apr 2024 13:45:43 GMT

←Older revision		Revision as of 13:45, 30 April 2024
Line 16:		Line 16:
	*Two Int3 protomers form a <scene name='10/1043660/Cv/4'>symmetrical dimer</scene> wherein two protomers are related by a non-crystallographic two-fold axis.		*Two Int3 protomers form a <scene name='10/1043660/Cv/4'>symmetrical dimer</scene> wherein two protomers are related by a non-crystallographic two-fold axis.
	*<scene name='10/1043660/Cv/8'>The dimer interface of Int3</scene>.		*<scene name='10/1043660/Cv/8'>The dimer interface of Int3</scene>.
		+	*<scene name='10/1043660/Cv/10'>The Int3/Int6 interface</scene>.

	==Integrator complex 3D structures==		==Integrator complex 3D structures==

Alexander Berchansky at 13:28, 30 April 2024

Alexander Berchansky — Tue, 30 Apr 2024 13:28:41 GMT

←Older revision		Revision as of 13:28, 30 April 2024
Line 15:		Line 15:

	*Two Int3 protomers form a <scene name='10/1043660/Cv/4'>symmetrical dimer</scene> wherein two protomers are related by a non-crystallographic two-fold axis.		*Two Int3 protomers form a <scene name='10/1043660/Cv/4'>symmetrical dimer</scene> wherein two protomers are related by a non-crystallographic two-fold axis.
		+	*<scene name='10/1043660/Cv/8'>The dimer interface of Int3</scene>.

	==Integrator complex 3D structures==		==Integrator complex 3D structures==

Alexander Berchansky at 12:30, 30 April 2024

Alexander Berchansky — Tue, 30 Apr 2024 12:30:47 GMT

←Older revision		Revision as of 12:30, 30 April 2024
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	== Function ==		== Function ==

-	'''Integrator complex''' (Int) is a protein complex which induces termination at loci transcribed by RNA polymerase II. Multiple Int subunits coordinate effective termination<ref>PMID:36634676</ref>. Int consists of 14 subunits some of which act as RNA endonuclease and are essential for small nuclear RNA biogenesis<ref>PMID:32800671</ref>. Int has protein phosphatase activity as well<ref>PMID:37995689</ref>.	+	'''Integrator complex''' (Int) is a protein complex which induces termination at loci transcribed by RNA polymerase II. Multiple Int subunits coordinate effective termination<ref>PMID:36634676</ref>. Int consists of 14 subunits some of which act as RNA endonuclease and are essential for small nuclear RNA biogenesis<ref>PMID:32800671</ref>. Int has protein phosphatase activity as well<ref>PMID:37995689</ref>.

	== Disease ==		== Disease ==
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	One of the pathways for fixing breaks in double-stranded DNA involve the participation of Int subunit 3 dimer and Int subunit 6. The 3D structure of the complex of the C-termini of Int3 dimer and Int6 shows the hydrophobic core interactions forming the Int3 dimer and the hydrophobic and ionic interactions between Int3 and Int6<ref>PMID:34400606</ref>.		One of the pathways for fixing breaks in double-stranded DNA involve the participation of Int subunit 3 dimer and Int subunit 6. The 3D structure of the complex of the C-termini of Int3 dimer and Int6 shows the hydrophobic core interactions forming the Int3 dimer and the hydrophobic and ionic interactions between Int3 and Int6<ref>PMID:34400606</ref>.

-	*Two Int3 protomers form a <scene name='10/1043660/Cv/3'>symmetrical dimer</scene> wherein two protomers are related by a non-crystallographic two-fold axis.	+	*Two Int3 protomers form a <scene name='10/1043660/Cv/4'>symmetrical dimer</scene> wherein two protomers are related by a non-crystallographic two-fold axis.

	==Integrator complex 3D structures==		==Integrator complex 3D structures==

Alexander Berchansky at 12:29, 30 April 2024

Alexander Berchansky — Tue, 30 Apr 2024 12:29:19 GMT

←Older revision		Revision as of 12:29, 30 April 2024
Line 13:		Line 13:

	One of the pathways for fixing breaks in double-stranded DNA involve the participation of Int subunit 3 dimer and Int subunit 6. The 3D structure of the complex of the C-termini of Int3 dimer and Int6 shows the hydrophobic core interactions forming the Int3 dimer and the hydrophobic and ionic interactions between Int3 and Int6<ref>PMID:34400606</ref>.		One of the pathways for fixing breaks in double-stranded DNA involve the participation of Int subunit 3 dimer and Int subunit 6. The 3D structure of the complex of the C-termini of Int3 dimer and Int6 shows the hydrophobic core interactions forming the Int3 dimer and the hydrophobic and ionic interactions between Int3 and Int6<ref>PMID:34400606</ref>.
		+
		+	*Two Int3 protomers form a <scene name='10/1043660/Cv/3'>symmetrical dimer</scene> wherein two protomers are related by a non-crystallographic two-fold axis.

	==Integrator complex 3D structures==		==Integrator complex 3D structures==

Alexander Berchansky at 12:23, 30 April 2024

Alexander Berchansky — Tue, 30 Apr 2024 12:23:29 GMT

←Older revision		Revision as of 12:23, 30 April 2024
Line 12:		Line 12:
	== Structural highlights ==		== Structural highlights ==

-	One of the pathways for fixing breaks n double-stranded DNA involve the participation of Int subunit 3 dimer and Int subunit 6. The 3D structure of the complex of the C-termini of Int3 dimer and Int6 shows the hydrophobic core interactions forming the Int3 dimer and the hydrophobic and ionic interactions between Int3 and Int6<ref>PMID:34400606</ref>.	+	One of the pathways for fixing breaks in double-stranded DNA involve the participation of Int subunit 3 dimer and Int subunit 6. The 3D structure of the complex of the C-termini of Int3 dimer and Int6 shows the hydrophobic core interactions forming the Int3 dimer and the hydrophobic and ionic interactions between Int3 and Int6<ref>PMID:34400606</ref>.

	==Integrator complex 3D structures==		==Integrator complex 3D structures==