Mannosidase - Revision history

Alexander Berchansky at 09:40, 25 December 2019

Alexander Berchansky — Wed, 25 Dec 2019 09:40:53 GMT

←Older revision		Revision as of 09:40, 25 December 2019
Line 3:		Line 3:
	== Function ==		== Function ==

-	'''Mannosidase''' (MAN) is an enzyme which hydrolyzes mannose. There are 2 kinds of MAN. '''α-MAN''' which hydrolyzes α-mannose<ref>PMID:12634058</ref> and '''β-MAN''' which hydrolyzes β-1,4-linked manno-oligosaccharides<ref>PMID:10553664</ref>. α-MAN isozymes are classified as class I and II. '''Mannan endo-1,4-β-MAN''' hydrolyzes 1-4-β-D-mannosidic linkages in mannans, galactomannans and glucomannans. See some details in [[Molecular Playground/ERMan1]].	+	'''Mannosidase''' (MAN) is an enzyme which hydrolyzes mannose. There are 2 kinds of MAN. '''α-MAN''' which hydrolyzes α-mannose<ref>PMID:12634058</ref> and '''β-MAN''' which hydrolyzes β-1,4-linked manno-oligosaccharides<ref>PMID:10553664</ref>. α-MAN isozymes are classified as class I and II. '''Mannan endo-1,4-β-MAN''' hydrolyzes 1-4-β-D-mannosidic linkages in mannans, galactomannans and glucomannans. See some details in [[Molecular Playground/ERMan1]]. See also [[Carbohydrate Metabolism]].

	== Disease ==		== Disease ==

Michal Harel at 09:56, 28 October 2019

Michal Harel — Mon, 28 Oct 2019 09:56:57 GMT

←Older revision		Revision as of 09:56, 28 October 2019
Line 15:		Line 15:

	</StructureSection>		</StructureSection>
-	==3D structures of mannosidase==
-	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
-	{{#tree:id=OrganizedByTopic\|openlevels=0\|

-	*α-MAN
-
-	**[[1o7d]] - αMAN – bovine<br />
-	**[[6b9o]] - jbMAN – jack bean<br />
-	**[[2wvy]], [[2ww2]], [[4acy]], [[4acz]], [[4ad0]], [[4c1s]] - αMAN - ''Bacterioides thetaiotaomicron''<br />
-	**[[1hcu]] - TrαMAN – ''Trichoderma reesei''<br />
-
-	*α-MAN class I
-
-	**[[1fmi]] - hαMAN I - human<br />
-	**[[1fo2]] - hαMAN I + deoxymannojirimycin<br />
-	**[[1fo3]] - hαMAN I+ kifunensine<br />
-	**[[1x9d]] - hαMAN I residues 243-699 + polysaccharide analog<br />
-	**[[1nxc]] - mαMAN I + mannose – mouse<br />
-	**[[6b9p]] - jbMAN + inhibitor<br />
-	**[[5jm0]] - yαMAN I – yeast<br />
-	**[[1dl2]] - yαMAN I catalytic domain + mannose <br />
-	**[[1g6i]] - yαMAN I + deoxymannojirimycin<br />
-	**[[2ri8]] - PcαMAN I + glycerol – ''Penicillium citrinum''<br />
-	**[[1kre]], [[1kkt]] - PcαMAN I + mannose<br />
-	**[[2ri9]] - PcαMAN I + substrate analog<br />
-	**[[1krf]] - PcαMAN I + kifunensine + mannose<br />
-
-
-	*α-MAN class II
-
-	**[[2wyh]] - SpαMAN II – ''Streptococcus pyrogenes''<br />
-	**[[3bub]], [[1hty]] - DmαMAN II catalytic domain – ''Drosophila melanogaster''<br />
-	**[[3bud]] - DmαMAN II catalytic domain (mutant) <br />
-	**[[3bui]] - DmαMAN II catalytic domain (mutant) + Tris<br />
-	**[[3bup]], [[3buq]], [[3czs]] - DmαMAN II catalytic domain (mutant) + mannose<br />
-	**[[3bvt]], [[3bvu]], [[3bvv]], [[3bvw]], [[3bvx]] - DmαMAN II catalytic domain (mutant) + mannopyranoside<br />
-	**[[1r33]], [[1r34]] - DmαMAN II catalytic domain + mannopyranoside<br />
-	**[[3ddf]], [[3ddg]], [[2f18]], [[2f1a]], [[2f1b]] - DmαMAN II catalytic domain + pyrrolidin<br />
-	**[[2alw]] - DmαMAN II catalytic domain + noeuromycin<br />
-	**[[3cv5]] - DmαMAN II catalytic domain + polysaccharide<br />
-	**[[3czn]] - DmαMAN II catalytic domain (mutant) + polysaccharide<br />
-	**[[2wyi]] - SpαMAN II + swainsonine<br />
-	**[[3blb]], [[1hww]] - DmαMAN II + swainsonine<br />
-	**[[3ejp]], [[3ejq]], [[3ejr]], [[3ejs]], [[3ejt]], [[3eju]], [[2ow6]], [[2ow7]] - DmαMAN II catalytic domain + swainsonine analog<br />
-	**[[3dx0]], [[3dx2]], [[2f7o]] - DmαMAN II catalytic domain + mannostatine<br />
-	**[[3dx1]], [[3dx3]], [[3dx4]], [[2f7p]] - DmαMAN II catalytic domain + mannostatine analog<br />
-	**[[3d4y]], [[3d4z]] - DmαMAN II catalytic domain + imidazole derivative<br />
-	**[[3d50]], [[3d51]], [[3d52]] - DmαMAN II catalytic domain + inhibitor<br />
-	**[[2fyv]], [[2f7q]], [[2f7r]] - DmαMAN II catalytic domain + amino-salacinol carboxylate analog<br />
-	**[[1tqv]], [[1tqw]], [[1tqs]], [[1tqt]], [[1tqu]] - DmαMAN II catalytic domain (mutant) + seleno-salacinol<br />
-	**[[1ps3]] - DmαMAN II catalytic domain + kifunensine<br />
-	**[[1qwn]] - DmαMAN II catalytic domain + intermediate<br />
-	**[[1hxk]] - DmαMAN II catalytic domain + deoxymannojirimycin<br />
-	**[[1qwu]] - DmαMAN II catalytic domain (mutant) + guloside<br />
-	**[[1qx1]] - DmαMAN II catalytic domain (mutant) + mannosyl
-
-	*α-1,2-MAN
-
-	**[[4ayo]] – CaαMAN - ''Caulobacter''<br />
-	**[[4ayp]], [[4ayq]], [[5meh]] – CaαMAN + mannose derivative<br />
-	**[[4ayr]] – CaαMAN + noeuromycin<br />
-	**[[5ne5]] – CaαMAN + kifunensine<br />
-	**[[5kkb]] – mαMAN + mannose derivative<br />
-	**[[5kij]] – hαMAN + mannose derivative<br />
-	**[[5kk7]] – hαMAN (mutant) + disaccharide derivative<br />
-
-	*β-MAN
-
-	**[[6dtt]] - mMAN <br />
-	**[[6ddu]] - mMAN + β-mannose <br />
-	**[[2je8]] - BtβMAN residues 26-864 <br />
-	**[[2wbk]] - BtβMAN residues 26-864 + mannopyranoside <br />
-	**[[2vqt]], [[2vr4]], [[2vjx]], [[2vl4]], [[2vmf]], [[2vo5]], [[2vot]], [[2vqu]] - BtβMAN residues 26-864 + piperidine derivative<br />
-	**[[1oh4]], [[1of4]] - TmβMAN catalytic domain + mannose – ''Thermotoga maritima''<br />
-	**[[1of3]] - TmβMAN catalytic domain<br />
-	**[[1uz4]] - CmβMAN + inhibitor – ''Cellvibrio mixtus''<br />
-	**[[1uuq]] - CmβMAN<br />
-	**[[2whk]] - βMAN – ''Bacillus subtilis''<br />
-	**[[4jho]] - rβMAN – rice<br />
-	**[[4jie]] - rβMAN + β-mannose<br />
-	**[[4cvu]], [[4uoj]] – βMAN - ''Trichoderma harzianum''<br />
-	**[[5n6u]] - βMAN + β-mannose – ''Dictyoglomus thermophilum''<br />
-	**[[6byc]] - XaMAN – ''Xanthomonas axonopodis''<br />
-	**[[6byg]], [[6byi]] - XaMAN (mutant)<br />
-	**[[6bye]] - XaMAN + β-mannose<br />
-	**[[4zxo]] - MAN – ''Bacterioides ovatus''<br />
-	**[[2whj]] - BaMAN – ''Bacillus agaradhaerens''<br />
-	**[[2whl]] - BaMAN + β-mannose <br />
-
-	*Mannan endo-1,4-β-MAN
-
-	**[[1gvy]], [[1odz]], [[1gw1]], [[2whm]] - PcβMAN (mutant) + mannose derivative – ''Pseudomonas cellulosa''<br />
-	**[[1j9y]], [[1r7o]] - PcβMAN<br />
-	**[[2c0h]] - βMAN (mutant) – blue mussel<br />
-	**[[2whk]], [[4yn5]], [[3jug]], [[2qha]] - βMAN – ''Bacillus subtilis''<br />
-	**[[1rh9]] – βMAN – ''Solanum lycopersicum''<br />
-	**[[3pz9]], [[3pzg]] – TpβMAN – ''Thermotoga petrophila''<br />
-	**[[3pzi]] – TpβMAN + glucose<br />
-	**[[3pzm]], [[3pzn]] – TpβMAN + glycerol<br />
-	**[[3pzo]], [[3pzq]] – TpβMAN + maltose<br />
-	**[[3ziz]] - βMAN – ''Podspora anserina''<br />
-	**[[3wh9]] - βMAN – ''Aspergillus niger''<br />
-	**[[1qns]], [[1qnr]], [[1qnq]], [[1qnp]], [[1qno]] - TrMAN <br />
-
-	*Endo-α-MAN
-
-	**[[4utf]] - BxαMAN – ''Bacterioides xylanisolvens''<br />
-	**[[4ad5]], [[4ad4]], [[4ad3]], [[4ad2]], [[4ad1]] – BxαMAN + mannose derivative<br />
-
-	*Exo-β-1,4-MAN
-
-	**[[4lyp]] - RmβMAN – ''Rhizomucor miehei''<br />
-	**[[4lyq]], [[4lyr]] - RmβMAN (mutant)<br />
-	**[[4nrr]] - RmβMAN (mutant) + mannosyl-fructose<br />
-	**[[4nrs]] - RmβMAN (mutant) + mannobiose<br />
-	**[[6gvb]] - βMAN – ''Cutibacterium acnes''<br />
-
-	*Exo-α-1,6-MAN
-
-	**[[5m7i]] - CpαMAN (mutant) + mannobiose – ''Clostridium perfringenes''<br />
-	**[[5m7y]] - CpαMAN (mutant) + mannotriose <br />
-	**[[6rqk]] - CpMAN + mannoimidazole<br />
-
-	*β-MAN/β-glucosidase
-
-	**[[4re2]], [[4re3]], [[4re4]] - rβMAN/βGLU <br />
-
-	}}
	== References ==		== References ==
	<references/>		<references/>

-
-
-
	[[Category:Topic Page]]		[[Category:Topic Page]]

Michal Harel at 09:55, 28 October 2019

Michal Harel — Mon, 28 Oct 2019 09:55:30 GMT

←Older revision		Revision as of 09:55, 28 October 2019
Line 10:		Line 10:
	== Structural highlights ==		== Structural highlights ==
	<scene name='45/458458/Cv/5'>The active site of β-MAN contains β-mannose</scene><ref>PMID:24100330</ref>. Water molecules shown as red spheres.		<scene name='45/458458/Cv/5'>The active site of β-MAN contains β-mannose</scene><ref>PMID:24100330</ref>. Water molecules shown as red spheres.
		+
		+	==3D structures of mannosidase==
		+	[[Mannosidase 3D structures]]
		+
	</StructureSection>		</StructureSection>
	==3D structures of mannosidase==		==3D structures of mannosidase==
Line 17:		Line 21:
	*α-MAN		*α-MAN

-	**[[1hcu]] - TrαMAN – ''Trichoderma reesei''<br />
	**[[1o7d]] - αMAN – bovine<br />		**[[1o7d]] - αMAN – bovine<br />
-	**[[2wvy]], [[2ww2]], [[4acy]], [[4acz]], [[4ad0]], [[4c1s]] - αMAN - ''Bacterioides thetaiotaomicron''	+	**[[6b9o]] - jbMAN – jack bean<br />
		+	**[[2wvy]], [[2ww2]], [[4acy]], [[4acz]], [[4ad0]], [[4c1s]] - αMAN - ''Bacterioides thetaiotaomicron''<br />
		+	**[[1hcu]] - TrαMAN – ''Trichoderma reesei''<br />

	*α-MAN class I		*α-MAN class I
Line 26:		Line 31:
	**[[1fo2]] - hαMAN I + deoxymannojirimycin<br />		**[[1fo2]] - hαMAN I + deoxymannojirimycin<br />
	**[[1fo3]] - hαMAN I+ kifunensine<br />		**[[1fo3]] - hαMAN I+ kifunensine<br />
		+	**[[1x9d]] - hαMAN I residues 243-699 + polysaccharide analog<br />
		+	**[[1nxc]] - mαMAN I + mannose – mouse<br />
		+	**[[6b9p]] - jbMAN + inhibitor<br />
	**[[5jm0]] - yαMAN I – yeast<br />		**[[5jm0]] - yαMAN I – yeast<br />
-	**[[1dl2]] - yαMAN I catalytic domain + mannose - ~~yeast~~<br />	+	**[[1dl2]] - yαMAN I catalytic domain + mannose <br />
		+	**[[1g6i]] - yαMAN I + deoxymannojirimycin<br />
	**[[2ri8]] - PcαMAN I + glycerol – ''Penicillium citrinum''<br />		**[[2ri8]] - PcαMAN I + glycerol – ''Penicillium citrinum''<br />
	**[[1kre]], [[1kkt]] - PcαMAN I + mannose<br />		**[[1kre]], [[1kkt]] - PcαMAN I + mannose<br />
	**[[2ri9]] - PcαMAN I + substrate analog<br />		**[[2ri9]] - PcαMAN I + substrate analog<br />
	**[[1krf]] - PcαMAN I + kifunensine + mannose<br />		**[[1krf]] - PcαMAN I + kifunensine + mannose<br />
-	**[[1x9d]] - hαMAN I residues 243-699 + polysaccharide analog<br />	+
-	**[[1nxc]] - mαMAN I + mannose – mouse<br />	+
-	**[[1g6i]] - yαMAN I + deoxymannojirimycin<br />	+

	*α-MAN class II		*α-MAN class II
Line 76:		Line 83:
	*β-MAN		*β-MAN

		+	**[[6dtt]] - mMAN <br />
		+	**[[6ddu]] - mMAN + β-mannose <br />
	**[[2je8]] - BtβMAN residues 26-864 <br />		**[[2je8]] - BtβMAN residues 26-864 <br />
	**[[2wbk]] - BtβMAN residues 26-864 + mannopyranoside <br />		**[[2wbk]] - BtβMAN residues 26-864 + mannopyranoside <br />
Line 89:		Line 98:
	**[[5n6u]] - βMAN + β-mannose – ''Dictyoglomus thermophilum''<br />		**[[5n6u]] - βMAN + β-mannose – ''Dictyoglomus thermophilum''<br />
	**[[6byc]] - XaMAN – ''Xanthomonas axonopodis''<br />		**[[6byc]] - XaMAN – ''Xanthomonas axonopodis''<br />
-	**[[6byg]], [[~~6bui~~]] - XaMAN (mutant)<br />	+	**[[6byg]], [[6byi]] - XaMAN (mutant)<br />
	**[[6bye]] - XaMAN + β-mannose<br />		**[[6bye]] - XaMAN + β-mannose<br />
	**[[4zxo]] - MAN – ''Bacterioides ovatus''<br />		**[[4zxo]] - MAN – ''Bacterioides ovatus''<br />
Line 121:		Line 130:
	**[[4nrr]] - RmβMAN (mutant) + mannosyl-fructose<br />		**[[4nrr]] - RmβMAN (mutant) + mannosyl-fructose<br />
	**[[4nrs]] - RmβMAN (mutant) + mannobiose<br />		**[[4nrs]] - RmβMAN (mutant) + mannobiose<br />
		+	**[[6gvb]] - βMAN – ''Cutibacterium acnes''<br />

	*Exo-α-1,6-MAN		*Exo-α-1,6-MAN

-	**[[5m7i]] - CpαMAN (mutant) + mannobiose – Clostridium perfringenes<br />	+	**[[5m7i]] - CpαMAN (mutant) + mannobiose – ''Clostridium perfringenes''<br />
-	**[[~~5m7i~~]] - CpαMAN (mutant) + mannotriose <br />	+	**[[5m7y]] - CpαMAN (mutant) + mannotriose <br />
		+	**[[6rqk]] - CpMAN + mannoimidazole<br />

	*β-MAN/β-glucosidase		*β-MAN/β-glucosidase

-	**[[4re2]], [[4re3]], [[4re4]] - rβMAN/βGLU ~~– rice~~<br />	+	**[[4re2]], [[4re3]], [[4re4]] - rβMAN/βGLU <br />

	}}		}}

Alexander Berchansky at 09:44, 10 July 2019

Alexander Berchansky — Wed, 10 Jul 2019 09:44:00 GMT

←Older revision		Revision as of 09:44, 10 July 2019
Line 9:		Line 9:

	== Structural highlights ==		== Structural highlights ==
-	<scene name='45/458458/Cv/4'>The active site of β-MAN contains β-mannose</scene><ref>PMID:24100330</ref>. Water molecules shown as red spheres.	+	<scene name='45/458458/Cv/5'>The active site of β-MAN contains β-mannose</scene><ref>PMID:24100330</ref>. Water molecules shown as red spheres.
	</StructureSection>		</StructureSection>
	==3D structures of mannosidase==		==3D structures of mannosidase==

Michal Harel at 08:06, 5 August 2018

Michal Harel — Sun, 05 Aug 2018 08:06:39 GMT

←Older revision		Revision as of 08:06, 5 August 2018
Line 17:		Line 17:
	*α-MAN		*α-MAN

-	**[[1hcu]] - ~~αMAN~~ – ''Trichoderma reesei''<br />	+	**[[1hcu]] - TrαMAN – ''Trichoderma reesei''<br />
	**[[1o7d]] - αMAN – bovine<br />		**[[1o7d]] - αMAN – bovine<br />
-	**[[2wvy]], [[2ww2]], [[4acy]], [[4acz]], [[4ad0]] - αMAN - ''Bacterioides thetaiotaomicron''	+	**[[2wvy]], [[2ww2]], [[4acy]], [[4acz]], [[4ad0]], [[4c1s]] - αMAN - ''Bacterioides thetaiotaomicron''

	*α-MAN class I		*α-MAN class I
Line 33:		Line 33:
	**[[1krf]] - PcαMAN I + kifunensine + mannose<br />		**[[1krf]] - PcαMAN I + kifunensine + mannose<br />
	**[[1x9d]] - hαMAN I residues 243-699 + polysaccharide analog<br />		**[[1x9d]] - hαMAN I residues 243-699 + polysaccharide analog<br />
-	**[[1nxc]] - ~~αMAN~~ I + mannose – mouse<br />	+	**[[1nxc]] - mαMAN I + mannose – mouse<br />
-	**[[1g6i]] - ~~αMAN~~ I + deoxymannojirimycin<br />	+	**[[1g6i]] - yαMAN I + deoxymannojirimycin<br />

	*α-MAN class II		*α-MAN class II
Line 67:		Line 67:

	**[[4ayo]] – CaαMAN - ''Caulobacter''<br />		**[[4ayo]] – CaαMAN - ''Caulobacter''<br />
-	**[[4ayp]], [[4ayq]] – CaαMAN + mannose derivative<br />	+	**[[4ayp]], [[4ayq]], [[5meh]] – CaαMAN + mannose derivative<br />
	**[[4ayr]] – CaαMAN + noeuromycin<br />		**[[4ayr]] – CaαMAN + noeuromycin<br />
		+	**[[5ne5]] – CaαMAN + kifunensine<br />
		+	**[[5kkb]] – mαMAN + mannose derivative<br />
		+	**[[5kij]] – hαMAN + mannose derivative<br />
		+	**[[5kk7]] – hαMAN (mutant) + disaccharide derivative<br />

	*β-MAN		*β-MAN
Line 84:		Line 88:
	**[[4cvu]], [[4uoj]] – βMAN - ''Trichoderma harzianum''<br />		**[[4cvu]], [[4uoj]] – βMAN - ''Trichoderma harzianum''<br />
	**[[5n6u]] - βMAN + β-mannose – ''Dictyoglomus thermophilum''<br />		**[[5n6u]] - βMAN + β-mannose – ''Dictyoglomus thermophilum''<br />
		+	**[[6byc]] - XaMAN – ''Xanthomonas axonopodis''<br />
		+	**[[6byg]], [[6bui]] - XaMAN (mutant)<br />
		+	**[[6bye]] - XaMAN + β-mannose<br />
		+	**[[4zxo]] - MAN – ''Bacterioides ovatus''<br />
		+	**[[2whj]] - BaMAN – ''Bacillus agaradhaerens''<br />
		+	**[[2whl]] - BaMAN + β-mannose <br />

	*Mannan endo-1,4-β-MAN		*Mannan endo-1,4-β-MAN
Line 90:		Line 100:
	**[[1j9y]], [[1r7o]] - PcβMAN<br />		**[[1j9y]], [[1r7o]] - PcβMAN<br />
	**[[2c0h]] - βMAN (mutant) – blue mussel<br />		**[[2c0h]] - βMAN (mutant) – blue mussel<br />
-	**[[2whk]], [[4yn5]] - βMAN – ''Bacillus subtilis''<br />	+	**[[2whk]], [[4yn5]], [[3jug]], [[2qha]] - βMAN – ''Bacillus subtilis''<br />
	**[[1rh9]] – βMAN – ''Solanum lycopersicum''<br />		**[[1rh9]] – βMAN – ''Solanum lycopersicum''<br />
	**[[3pz9]], [[3pzg]] – TpβMAN – ''Thermotoga petrophila''<br />		**[[3pz9]], [[3pzg]] – TpβMAN – ''Thermotoga petrophila''<br />
	**[[3pzi]] – TpβMAN + glucose<br />		**[[3pzi]] – TpβMAN + glucose<br />
	**[[3pzm]], [[3pzn]] – TpβMAN + glycerol<br />		**[[3pzm]], [[3pzn]] – TpβMAN + glycerol<br />
-	**[[3pzo]], [[3pzq]] – TpβMAN + maltose	+	**[[3pzo]], [[3pzq]] – TpβMAN + maltose<br />
		+	**[[3ziz]] - βMAN – ''Podspora anserina''<br />
		+	**[[3wh9]] - βMAN – ''Aspergillus niger''<br />
		+	**[[1qns]], [[1qnr]], [[1qnq]], [[1qnp]], [[1qno]] - TrMAN <br />
		+
		+	*Endo-α-MAN
		+
		+	**[[4utf]] - BxαMAN – ''Bacterioides xylanisolvens''<br />
		+	**[[4ad5]], [[4ad4]], [[4ad3]], [[4ad2]], [[4ad1]] – BxαMAN + mannose derivative<br />

	*Exo-β-1,4-MAN		*Exo-β-1,4-MAN

-	**[[4lyp]] - RmβMAN – Rhizomucor miehei<br />	+	**[[4lyp]] - RmβMAN – ''Rhizomucor miehei''<br />
	**[[4lyq]], [[4lyr]] - RmβMAN (mutant)<br />		**[[4lyq]], [[4lyr]] - RmβMAN (mutant)<br />
	**[[4nrr]] - RmβMAN (mutant) + mannosyl-fructose<br />		**[[4nrr]] - RmβMAN (mutant) + mannosyl-fructose<br />

Michal Harel at 07:55, 10 July 2017

Michal Harel — Mon, 10 Jul 2017 07:55:35 GMT

←Older revision		Revision as of 07:55, 10 July 2017
Line 111:		Line 111:
	*β-MAN/β-glucosidase		*β-MAN/β-glucosidase

-	**[[4re2, [[4re3]], [[4re4]] - rβMAN/βGLU – rice<br />	+	**[[4re2]], [[4re3]], [[4re4]] - rβMAN/βGLU – rice<br />

	}}		}}

Michal Harel at 07:54, 10 July 2017

Michal Harel — Mon, 10 Jul 2017 07:54:31 GMT

←Older revision		Revision as of 07:54, 10 July 2017
Line 1:		Line 1:
-	<StructureSection load='4jie' size='~~450~~' side='right' caption='Rice β-mannosidase complex with β-mannose, glycerol and HEPES (PDB entry [[4jie]])' scene='45/458458/Cv/1'>	+	<StructureSection load='4jie' size='350' side='right' caption='Rice β-mannosidase complex with β-mannose, glycerol and HEPES (PDB entry [[4jie]])' scene='45/458458/Cv/1'>
	__TOC__		__TOC__
	== Function ==		== Function ==
Line 26:		Line 26:
	**[[1fo2]] - hαMAN I + deoxymannojirimycin<br />		**[[1fo2]] - hαMAN I + deoxymannojirimycin<br />
	**[[1fo3]] - hαMAN I+ kifunensine<br />		**[[1fo3]] - hαMAN I+ kifunensine<br />
-	**[[1dl2]] - ~~αMAN~~ I catalytic domain + mannose - yeast<br />	+	**[[5jm0]] - yαMAN I – yeast<br />
		+	**[[1dl2]] - yαMAN I catalytic domain + mannose - yeast<br />
	**[[2ri8]] - PcαMAN I + glycerol – ''Penicillium citrinum''<br />		**[[2ri8]] - PcαMAN I + glycerol – ''Penicillium citrinum''<br />
	**[[1kre]], [[1kkt]] - PcαMAN I + mannose<br />		**[[1kre]], [[1kkt]] - PcαMAN I + mannose<br />
Line 81:		Line 82:
	**[[4jho]] - rβMAN – rice<br />		**[[4jho]] - rβMAN – rice<br />
	**[[4jie]] - rβMAN + β-mannose<br />		**[[4jie]] - rβMAN + β-mannose<br />
		+	**[[4cvu]], [[4uoj]] – βMAN - ''Trichoderma harzianum''<br />
		+	**[[5n6u]] - βMAN + β-mannose – ''Dictyoglomus thermophilum''<br />

	*Mannan endo-1,4-β-MAN		*Mannan endo-1,4-β-MAN
Line 87:		Line 90:
	**[[1j9y]], [[1r7o]] - PcβMAN<br />		**[[1j9y]], [[1r7o]] - PcβMAN<br />
	**[[2c0h]] - βMAN (mutant) – blue mussel<br />		**[[2c0h]] - βMAN (mutant) – blue mussel<br />
-	**[[2whk]] - βMAN – ''Bacillus subtilis''<br />	+	**[[2whk]], [[4yn5]] - βMAN – ''Bacillus subtilis''<br />
	**[[1rh9]] – βMAN – ''Solanum lycopersicum''<br />		**[[1rh9]] – βMAN – ''Solanum lycopersicum''<br />
	**[[3pz9]], [[3pzg]] – TpβMAN – ''Thermotoga petrophila''<br />		**[[3pz9]], [[3pzg]] – TpβMAN – ''Thermotoga petrophila''<br />
Line 93:		Line 96:
	**[[3pzm]], [[3pzn]] – TpβMAN + glycerol<br />		**[[3pzm]], [[3pzn]] – TpβMAN + glycerol<br />
	**[[3pzo]], [[3pzq]] – TpβMAN + maltose		**[[3pzo]], [[3pzq]] – TpβMAN + maltose
		+
		+	*Exo-β-1,4-MAN
		+
		+	**[[4lyp]] - RmβMAN – Rhizomucor miehei<br />
		+	**[[4lyq]], [[4lyr]] - RmβMAN (mutant)<br />
		+	**[[4nrr]] - RmβMAN (mutant) + mannosyl-fructose<br />
		+	**[[4nrs]] - RmβMAN (mutant) + mannobiose<br />
		+
		+	*Exo-α-1,6-MAN
		+
		+	**[[5m7i]] - CpαMAN (mutant) + mannobiose – Clostridium perfringenes<br />
		+	**[[5m7i]] - CpαMAN (mutant) + mannotriose <br />
		+
		+	*β-MAN/β-glucosidase
		+
		+	**[[4re2, [[4re3]], [[4re4]] - rβMAN/βGLU – rice<br />

	}}		}}

Alexander Berchansky at 11:15, 18 April 2016

Alexander Berchansky — Mon, 18 Apr 2016 11:15:02 GMT

←Older revision		Revision as of 11:15, 18 April 2016
Line 1:		Line 1:
-	<StructureSection load='4jie' size='~~350~~' side='right' caption='Rice β-mannosidase complex with β-mannose, glycerol and HEPES (PDB entry [[4jie]])' scene='45/458458/Cv/1'>	+	<StructureSection load='4jie' size='450' side='right' caption='Rice β-mannosidase complex with β-mannose, glycerol and HEPES (PDB entry [[4jie]])' scene='45/458458/Cv/1'>
	__TOC__		__TOC__
	== Function ==		== Function ==

Alexander Berchansky at 11:13, 18 April 2016

Alexander Berchansky — Mon, 18 Apr 2016 11:13:29 GMT

←Older revision		Revision as of 11:13, 18 April 2016
Line 9:		Line 9:

	== Structural highlights ==		== Structural highlights ==
-	The active site of β-MAN contains β-mannose<ref>PMID:24100330</ref>.	+	<scene name='45/458458/Cv/4'>The active site of β-MAN contains β-mannose</scene><ref>PMID:24100330</ref>. Water molecules shown as red spheres.
	</StructureSection>		</StructureSection>
	==3D structures of mannosidase==		==3D structures of mannosidase==

Alexander Berchansky at 10:52, 18 April 2016

Alexander Berchansky — Mon, 18 Apr 2016 10:52:48 GMT

←Older revision		Revision as of 10:52, 18 April 2016
Line 1:		Line 1:
-	<StructureSection load='4jie' size='350' side='right' caption='Rice β-mannosidase complex with β-mannose, glycerol and ~~hepes~~ (PDB entry [[4jie]])' scene=''>	+	<StructureSection load='4jie' size='350' side='right' caption='Rice β-mannosidase complex with β-mannose, glycerol and HEPES (PDB entry [[4jie]])' scene='45/458458/Cv/1'>
	__TOC__		__TOC__
	== Function ==		== Function ==