NADPH-Cytochrome P450 Reductase - Revision history

Michal Harel at 09:24, 11 July 2023

Michal Harel — Tue, 11 Jul 2023 09:24:27 GMT

←Older revision		Revision as of 09:24, 11 July 2023
Line 51:		Line 51:
	*P450r		*P450r

		+	**[[7sux]], [[7sv0]] – sP450r + FAD + NADP - sorghum<br />
		+	**[[7suz]] – sP450r + FAD <br />
	**[[5ucw]] - P450r (mutant) – ''Bacillus megaterium''<br />		**[[5ucw]] - P450r (mutant) – ''Bacillus megaterium''<br />
	**[[5gxu]] - P450r + FAD + FMN – ''Arabidopsis thaliana''<br />		**[[5gxu]] - P450r + FAD + FMN – ''Arabidopsis thaliana''<br />

Michal Harel at 10:38, 16 November 2021

Michal Harel — Tue, 16 Nov 2021 10:38:35 GMT

←Older revision		Revision as of 10:38, 16 November 2021
Line 38:		Line 38:
	**[[3qfc]], [[3qfr]], [[5emn]] – hP450r (mutant) + FAD + FMN + NADP<br />		**[[3qfc]], [[3qfr]], [[5emn]] – hP450r (mutant) + FAD + FMN + NADP<br />
	**[[3es9]], [[1ja0]], [[5urd]], [[5ure]], [[5urg]] - rP450r + FAD + FMN + NADP - rat<br />		**[[3es9]], [[1ja0]], [[5urd]], [[5ure]], [[5urg]] - rP450r + FAD + FMN + NADP - rat<br />
-	**[[1j9z]], [[1ja1]], [[4y7c]], [[4y9r]], [[4y9u]], [[5urh]], [[6njr]] - rP450r (mutant) + FAD + FMN + NADP<br / >	+	**[[1j9z]], [[1ja1]], [[4y7c]], [[4y9r]], [[4y9u]], [[5urh]], [[6njr]], [[7l18]] - rP450r (mutant) + FAD + FMN + NADP<br / >
	**[[4yaf]], [[4yal]] - rP450r + FAD + FMN + AMP<br / >		**[[4yaf]], [[4yal]] - rP450r + FAD + FMN + AMP<br / >
	**[[4yaw]], [[4yao]], [[4yau]], [[5uri]] - rP450r (mutant) + FAD + FMN + AMP<br / >		**[[4yaw]], [[4yao]], [[4yau]], [[5uri]] - rP450r (mutant) + FAD + FMN + AMP<br / >

Michal Harel at 07:37, 10 September 2020

Michal Harel — Thu, 10 Sep 2020 07:37:36 GMT

←Older revision		Revision as of 07:37, 10 September 2020
Line 44:		Line 44:
	**[[3wkt]] - rP450r (mutant) + heme oxygenase 1 + FAD + FMN + NADP + heme<br />		**[[3wkt]] - rP450r (mutant) + heme oxygenase 1 + FAD + FMN + NADP + heme<br />
	**[[1amo]] - rP450r + FAD + FMN + NADP<br />		**[[1amo]] - rP450r + FAD + FMN + NADP<br />
		+	**[[6njr]] - rP450r (mutant) + FAD + FMN + NADP + pyrrolydine derivative<br />
	**[[2bpo]] - yP450r (mutant) + FAD + FMN + NADP - yeast<br />		**[[2bpo]] - yP450r (mutant) + FAD + FMN + NADP - yeast<br />
	**[[2bf4]], [[2bn4]] - yP450r + FAD + FMN + NADP<br />		**[[2bf4]], [[2bn4]] - yP450r + FAD + FMN + NADP<br />
Line 51:		Line 52:

	**[[5ucw]] - P450r (mutant) – ''Bacillus megaterium''<br />		**[[5ucw]] - P450r (mutant) – ''Bacillus megaterium''<br />
-	**[[5gxu]] - P450r + FAD + FMN – ''Arabidopsis thaliana''''~~Italic text~~''<br />	+	**[[5gxu]] - P450r + FAD + FMN – ''Arabidopsis thaliana''<br />
		+	**[[6t1u]] - CtP450r + FAD + FMN – ''Candida tropicalis''<br />
		+	**[[6t1t]] - CtP450r + FAD + FMN + NADP <br />

	}}		}}

Michal Harel at 07:39, 6 November 2019

Michal Harel — Wed, 06 Nov 2019 07:39:25 GMT

←Older revision		Revision as of 07:39, 6 November 2019
Line 35:		Line 35:
	*P450r FMN-binding and FAD/NADPH domains (residues 64-677)		*P450r FMN-binding and FAD/NADPH domains (residues 64-677)

-	**[[~~2bpo~~]] ~~- yP450r (mutant)~~ + FAD + FMN + NADP ~~- yeast~~<br />	+	**[[3qe2]], [[5fa6]] – hP450r + FAD + FMN + NADP<br />
-	**[[~~2bf4~~]], [[~~2bn4~~]] ~~- yP450r~~ + FAD + FMN + NADP<br />	+	**[[3qfc]], [[3qfr]], [[5emn]] – hP450r (mutant) + FAD + FMN + NADP<br />
	**[[3es9]], [[1ja0]], [[5urd]], [[5ure]], [[5urg]] - rP450r + FAD + FMN + NADP - rat<br />		**[[3es9]], [[1ja0]], [[5urd]], [[5ure]], [[5urg]] - rP450r + FAD + FMN + NADP - rat<br />
-	**[[1j9z]], [[1ja1]], [[4y7c]], [[4y9r]], [[4y9u]], [[5urh]] - rP450r (mutant) + FAD + FMN + NADP<br / >	+	**[[1j9z]], [[1ja1]], [[4y7c]], [[4y9r]], [[4y9u]], [[5urh]], [[6njr]] - rP450r (mutant) + FAD + FMN + NADP<br / >
	**[[4yaf]], [[4yal]] - rP450r + FAD + FMN + AMP<br / >		**[[4yaf]], [[4yal]] - rP450r + FAD + FMN + AMP<br / >
	**[[4yaw]], [[4yao]], [[4yau]], [[5uri]] - rP450r (mutant) + FAD + FMN + AMP<br / >		**[[4yaw]], [[4yao]], [[4yau]], [[5uri]] - rP450r (mutant) + FAD + FMN + AMP<br / >
Line 44:		Line 44:
	**[[3wkt]] - rP450r (mutant) + heme oxygenase 1 + FAD + FMN + NADP + heme<br />		**[[3wkt]] - rP450r (mutant) + heme oxygenase 1 + FAD + FMN + NADP + heme<br />
	**[[1amo]] - rP450r + FAD + FMN + NADP<br />		**[[1amo]] - rP450r + FAD + FMN + NADP<br />
-	**[[~~3qe2~~]]~~, [[5fa6]] – hP450r~~ + FAD + FMN + NADP<br />	+	**[[2bpo]] - yP450r (mutant) + FAD + FMN + NADP - yeast<br />
-	**[[~~3qfc~~]], [[~~3qfr~~]]~~, [[5emn]] – hP450r (mutant)~~ + FAD + FMN + NADP<br />	+	**[[2bf4]], [[2bn4]] - yP450r + FAD + FMN + NADP<br />
	**[[3fjo]] – yP450r/hP450r + FAD + FMN<br />		**[[3fjo]] – yP450r/hP450r + FAD + FMN<br />

Alexander Berchansky at 12:49, 22 July 2019

Alexander Berchansky — Mon, 22 Jul 2019 12:49:25 GMT

←Older revision		Revision as of 12:49, 22 July 2019
Line 11:		Line 11:

	== Structural highlights ==		== Structural highlights ==
-	P450r electron transfer cofactors: <scene name='43/436100/Cv/3'>FMN, FAD, NADPH are all in proximity</scene><ref>PMID:21808038</ref>.	+	P450r electron transfer cofactors: <scene name='43/436100/Cv/18'>FMN, FAD, NADPH are all in proximity</scene><ref>PMID:21808038</ref>.
-	*<scene name='43/436100/Cv/5'>NADP binding site</scene>. Water molecule shown as red spheres.	+	*<scene name='43/436100/Cv/19'>NADP binding site</scene>. Water molecule are shown as red spheres.
-	*<scene name='43/436100/Cv/16'>FAD/NADP connection via Thr538 and water molecule</scene>.	+	*<scene name='43/436100/Cv/20'>FAD/NADP connection via Thr538 and water molecule</scene>.
-	*<scene name='43/436100/Cv/17'>FAD binding site</scene>.	+	*<scene name='43/436100/Cv/21'>FAD binding site</scene>.
-	*<scene name='43/436100/Cv/13'>FAD and FMN are also in proximity</scene>.	+	*<scene name='43/436100/Cv/22'>FAD and FMN are also in proximity</scene>.
-	*<scene name='43/436100/Cv/12'>FMN binding site</scene>.	+	*<scene name='43/436100/Cv/23'>FMN binding site</scene>.
-	*<scene name='43/436100/Cv/14'>NADP and FAD are in pockets, while FMN is in tunnel</scene>.	+	*<scene name='43/436100/Cv/24'>NADP and FAD are in pockets, while FMN is in tunnel</scene>.
	</StructureSection>		</StructureSection>
	== 3D Structures of NADPH-Cytochrome P450 Reductase ==		== 3D Structures of NADPH-Cytochrome P450 Reductase ==

Michal Harel at 09:36, 12 August 2018

Michal Harel — Sun, 12 Aug 2018 09:36:14 GMT

←Older revision		Revision as of 09:36, 12 August 2018
Line 38:		Line 38:
	**[[2bf4]], [[2bn4]] - yP450r + FAD + FMN + NADP<br />		**[[2bf4]], [[2bn4]] - yP450r + FAD + FMN + NADP<br />
	**[[3es9]], [[1ja0]], [[5urd]], [[5ure]], [[5urg]] - rP450r + FAD + FMN + NADP - rat<br />		**[[3es9]], [[1ja0]], [[5urd]], [[5ure]], [[5urg]] - rP450r + FAD + FMN + NADP - rat<br />
-	**[[1j9z]], [[1ja1]], [[4y7c]], [[4y9r]], [[4y9u]] - rP450r (mutant) + FAD + FMN + NADP<br / >	+	**[[1j9z]], [[1ja1]], [[4y7c]], [[4y9r]], [[4y9u]], [[5urh]] - rP450r (mutant) + FAD + FMN + NADP<br / >
	**[[4yaf]], [[4yal]] - rP450r + FAD + FMN + AMP<br / >		**[[4yaf]], [[4yal]] - rP450r + FAD + FMN + AMP<br / >
-	**[[4yaw]], [[4yao]], [[4yau]] - rP450r (mutant) + FAD + FMN + AMP<br / >	+	**[[4yaw]], [[4yao]], [[4yau]], [[5uri]] - rP450r (mutant) + FAD + FMN + AMP<br / >
	**[[3ojw]], [[3ojx]] - rP450r (mutant) + FAD + FMN<br / >		**[[3ojw]], [[3ojx]] - rP450r (mutant) + FAD + FMN<br / >
	**[[3wkt]] - rP450r (mutant) + heme oxygenase 1 + FAD + FMN + NADP + heme<br />		**[[3wkt]] - rP450r (mutant) + heme oxygenase 1 + FAD + FMN + NADP + heme<br />

Michal Harel at 09:34, 12 August 2018

Michal Harel — Sun, 12 Aug 2018 09:34:36 GMT

←Older revision		Revision as of 09:34, 12 August 2018
Line 37:		Line 37:
	**[[2bpo]] - yP450r (mutant) + FAD + FMN + NADP - yeast<br />		**[[2bpo]] - yP450r (mutant) + FAD + FMN + NADP - yeast<br />
	**[[2bf4]], [[2bn4]] - yP450r + FAD + FMN + NADP<br />		**[[2bf4]], [[2bn4]] - yP450r + FAD + FMN + NADP<br />
-	**[[3es9]], [[1ja0]] - rP450r + FAD + FMN + NADP - rat<br />	+	**[[3es9]], [[1ja0]], [[5urd]], [[5ure]], [[5urg]] - rP450r + FAD + FMN + NADP - rat<br />
	**[[1j9z]], [[1ja1]], [[4y7c]], [[4y9r]], [[4y9u]] - rP450r (mutant) + FAD + FMN + NADP<br / >		**[[1j9z]], [[1ja1]], [[4y7c]], [[4y9r]], [[4y9u]] - rP450r (mutant) + FAD + FMN + NADP<br / >
	**[[4yaf]], [[4yal]] - rP450r + FAD + FMN + AMP<br / >		**[[4yaf]], [[4yal]] - rP450r + FAD + FMN + AMP<br / >

Michal Harel at 09:26, 12 August 2018

Michal Harel — Sun, 12 Aug 2018 09:26:09 GMT

←Older revision		Revision as of 09:26, 12 August 2018
Line 1:		Line 1:
-	<StructureSection load='' size='~~450~~' side='right' caption='Human NADPH-Cytochrome P450 reductase FMN-binding and FAD/NADPH domains complex with FMN, FAD, NADP and Ca+2 ion (green) (PDB entry [[3qe2]])' scene='43/436100/Cv/2'>	+	<StructureSection load='' size='350' side='right' caption='Human NADPH-Cytochrome P450 reductase FMN-binding and FAD/NADPH domains complex with FMN, FAD, NADP and Ca+2 ion (green) (PDB entry [[3qe2]])' scene='43/436100/Cv/2'>
	__TOC__		__TOC__
	== Function ==		== Function ==

Michal Harel at 09:40, 2 January 2018

Michal Harel — Tue, 02 Jan 2018 09:40:00 GMT

←Older revision		Revision as of 09:40, 2 January 2018
Line 3:		Line 3:
	== Function ==		== Function ==

-	[[NADPH-Cytochrome P450 Reductase]] (P450r) is the electron donor for several oxygenase enzymes including Cytochrome P450<ref>PMID:19171935</ref>.	+	[[NADPH-Cytochrome P450 Reductase]] (P450r) or '''NADPH cytochrome P450 oxidoreductase''' is the electron donor for several oxygenase enzymes including Cytochrome P450<ref>PMID:19171935</ref>.

	See also [[NADPH Cytochrome P450 Oxidoreductase]].		See also [[NADPH Cytochrome P450 Oxidoreductase]].

Michal Harel at 07:08, 21 August 2017

Michal Harel — Mon, 21 Aug 2017 07:08:23 GMT

←Older revision		Revision as of 07:08, 21 August 2017
Line 38:		Line 38:
	**[[2bf4]], [[2bn4]] - yP450r + FAD + FMN + NADP<br />		**[[2bf4]], [[2bn4]] - yP450r + FAD + FMN + NADP<br />
	**[[3es9]], [[1ja0]] - rP450r + FAD + FMN + NADP - rat<br />		**[[3es9]], [[1ja0]] - rP450r + FAD + FMN + NADP - rat<br />
-	**[[1j9z]], [[1ja1]] - rP450r (mutant) + FAD + FMN + NADP<br / >	+	**[[1j9z]], [[1ja1]], [[4y7c]], [[4y9r]], [[4y9u]] - rP450r (mutant) + FAD + FMN + NADP<br / >
		+	**[[4yaf]], [[4yal]] - rP450r + FAD + FMN + AMP<br / >
		+	**[[4yaw]], [[4yao]], [[4yau]] - rP450r (mutant) + FAD + FMN + AMP<br / >
	**[[3ojw]], [[3ojx]] - rP450r (mutant) + FAD + FMN<br / >		**[[3ojw]], [[3ojx]] - rP450r (mutant) + FAD + FMN<br / >
	**[[3wkt]] - rP450r (mutant) + heme oxygenase 1 + FAD + FMN + NADP + heme<br />		**[[3wkt]] - rP450r (mutant) + heme oxygenase 1 + FAD + FMN + NADP + heme<br />
	**[[1amo]] - rP450r + FAD + FMN + NADP<br />		**[[1amo]] - rP450r + FAD + FMN + NADP<br />
-	**[[3qe2]] – hP450r + FAD + FMN + NADP<br />	+	**[[3qe2]], [[5fa6]] – hP450r + FAD + FMN + NADP<br />
-	**[[3qfc]], [[3qfr]] – hP450r (mutant) + FAD + FMN + NADP<br />	+	**[[3qfc]], [[3qfr]], [[5emn]] – hP450r (mutant) + FAD + FMN + NADP<br />
	**[[3fjo]] – yP450r/hP450r + FAD + FMN<br />		**[[3fjo]] – yP450r/hP450r + FAD + FMN<br />
		+
		+	*P450r
		+
		+	**[[5ucw]] - P450r (mutant) – ''Bacillus megaterium''<br />
		+	**[[5gxu]] - P450r + FAD + FMN – ''Arabidopsis thaliana''''Italic text''<br />

	}}		}}