Priyanka Basak/Sandbox - Revision history

Priyanka Basak at 20:40, 2 December 2014

Priyanka Basak — Tue, 02 Dec 2014 20:40:51 GMT

←Older revision		Revision as of 20:40, 2 December 2014
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	==Introduction==		==Introduction==
-	<StructureSection load='3tj8' size='340' side='right' caption='UreE' scene=''>	+	<StructureSection load='3tj8' size='340' side='right' caption='''Hp''UreE,PDB ID: 1TJ8 ' scene=''>
	UreE is an essential metallochaeprone for ''Helicobacter pylori'' (''H.pylori''). ''H.pylori'' is a class I human pathogen that is involved in causing gastric ulcers and stomach cancers. Its two nickel dependent enzymes - Urease and NiFe-H2ase, provides it a unique ability to survive and colonize in acidic human stomach. Urease catalyzes the conversion of urea to ammonia and carbamate which neutralizes its local acidic environment.		UreE is an essential metallochaeprone for ''Helicobacter pylori'' (''H.pylori''). ''H.pylori'' is a class I human pathogen that is involved in causing gastric ulcers and stomach cancers. Its two nickel dependent enzymes - Urease and NiFe-H2ase, provides it a unique ability to survive and colonize in acidic human stomach. Urease catalyzes the conversion of urea to ammonia and carbamate which neutralizes its local acidic environment.
	== Function ==		== Function ==

Priyanka Basak at 20:30, 2 December 2014

Priyanka Basak — Tue, 02 Dec 2014 20:30:56 GMT

←Older revision		Revision as of 20:30, 2 December 2014
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	Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.		Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.
	== Structural highlights ==		== Structural highlights ==
-	Apo-UreE from ''H.pylori'' is a dimer.~~[[Image:HpUreE.png size='300' side='right']]~~''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer).	+	Apo-UreE from ''H.pylori'' is a dimer.''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer).

Priyanka Basak at 20:30, 2 December 2014

Priyanka Basak — Tue, 02 Dec 2014 20:30:32 GMT

←Older revision		Revision as of 20:30, 2 December 2014
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	Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.		Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.
	== Structural highlights ==		== Structural highlights ==
-	Apo-UreE from ''H.pylori'' is a dimer.''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer).	+	Apo-UreE from ''H.pylori'' is a dimer.[[Image:HpUreE.png size='300' side='right']]''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer).

Priyanka Basak at 20:27, 2 December 2014

Priyanka Basak — Tue, 02 Dec 2014 20:27:12 GMT

←Older revision		Revision as of 20:27, 2 December 2014
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	Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.		Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.
	== Structural highlights ==		== Structural highlights ==
-	Apo-UreE from ''H.pylori'' is a dimer.~~[[Image:HpUreE.png]]~~ ''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer).	+	Apo-UreE from ''H.pylori'' is a dimer.''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer).

Priyanka Basak at 20:26, 2 December 2014

Priyanka Basak — Tue, 02 Dec 2014 20:26:04 GMT

←Older revision		Revision as of 20:26, 2 December 2014
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	Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.		Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.
	== Structural highlights ==		== Structural highlights ==
-	Apo-UreE from ''H.pylori'' is a dimer.[[Image:HpUreE.png~~/300px/left~~]] ''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer).	+	Apo-UreE from ''H.pylori'' is a dimer.[[Image:HpUreE.png]] ''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer).

Priyanka Basak at 20:25, 2 December 2014

Priyanka Basak — Tue, 02 Dec 2014 20:25:45 GMT

←Older revision		Revision as of 20:25, 2 December 2014
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	Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.		Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.
	== Structural highlights ==		== Structural highlights ==
-	Apo-UreE from ''H.pylori'' is a dimer.[[Image:HpUreE.png//300px/~~right/~~]] ''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer).	+	Apo-UreE from ''H.pylori'' is a dimer.[[Image:HpUreE.png/300px/left]] ''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer).

Priyanka Basak at 20:23, 2 December 2014

Priyanka Basak — Tue, 02 Dec 2014 20:23:25 GMT

←Older revision		Revision as of 20:23, 2 December 2014
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	Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.		Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.
	== Structural highlights ==		== Structural highlights ==
-	Apo-UreE from ''H.pylori'' is a dimer.[[Image:HpUreE.png]] ''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer).	+	Apo-UreE from ''H.pylori'' is a dimer.[[Image:HpUreE.png//300px/right/]] ''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer).

Priyanka Basak at 20:17, 2 December 2014

Priyanka Basak — Tue, 02 Dec 2014 20:17:12 GMT

←Older revision		Revision as of 20:17, 2 December 2014
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	Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.		Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.
	== Structural highlights ==		== Structural highlights ==
-	Apo-UreE from ''H.pylori'' is a dimer. ''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer).	+	Apo-UreE from ''H.pylori'' is a dimer.[[Image:HpUreE.png]] ''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer).

Priyanka Basak at 20:07, 2 December 2014

Priyanka Basak — Tue, 02 Dec 2014 20:07:03 GMT

←Older revision		Revision as of 20:07, 2 December 2014
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	Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.		Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.
	== Structural highlights ==		== Structural highlights ==
-	Apo-UreE from ''H.pylori'' is a dimer ~~which becomes tetrameric (dimer of dimer) when bound to metal~~. ''Hp''UreE can bind one Ni(II) <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.	+	Apo-UreE from ''H.pylori'' is a dimer. ''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer).

Priyanka Basak at 20:02, 2 December 2014

Priyanka Basak — Tue, 02 Dec 2014 20:02:42 GMT

←Older revision		Revision as of 20:02, 2 December 2014
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	UreE is an essential metallochaeprone for ''Helicobacter pylori'' (''H.pylori''). ''H.pylori'' is a class I human pathogen that is involved in causing gastric ulcers and stomach cancers. Its two nickel dependent enzymes - Urease and NiFe-H2ase, provides it a unique ability to survive and colonize in acidic human stomach. Urease catalyzes the conversion of urea to ammonia and carbamate which neutralizes its local acidic environment.		UreE is an essential metallochaeprone for ''Helicobacter pylori'' (''H.pylori''). ''H.pylori'' is a class I human pathogen that is involved in causing gastric ulcers and stomach cancers. Its two nickel dependent enzymes - Urease and NiFe-H2ase, provides it a unique ability to survive and colonize in acidic human stomach. Urease catalyzes the conversion of urea to ammonia and carbamate which neutralizes its local acidic environment.
	== Function ==		== Function ==
-	Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific ~~Nickel~~ metallochaperone that is involved in the delivery nickel to urease.	+	Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.
	== Structural highlights ==		== Structural highlights ==
-	Apo-UreE from ''H.pylori'' is a dimer which becomes tetrameric (dimer of dimer) when bound to metal. ''Hp''UreE can bind one Ni(II) <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound to UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>UreE-Nickel Binding site</scene>.	+	Apo-UreE from ''H.pylori'' is a dimer which becomes tetrameric (dimer of dimer) when bound to metal. ''Hp''UreE can bind one Ni(II) <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.