Rabphilin - Revision history

Michal Harel at 10:15, 5 October 2023

Michal Harel — Thu, 05 Oct 2023 10:15:39 GMT

←Older revision		Revision as of 10:15, 5 October 2023
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	[[2cm5]], [[2cm6]] – rRab C2B domain – rat<br />		[[2cm5]], [[2cm6]] – rRab C2B domain – rat<br />
-	[[4izk]] – rRab C2B domain - NMR<br />	+	[[3rpb]], [[4izk]] – rRab C2B domain - NMR<br />
	[[5lo8]] – rRab C2B domain + inositol phosphate derivative<br />		[[5lo8]] – rRab C2B domain + inositol phosphate derivative<br />
	[[5lob]], [[5low]] – rRab C2B domain + SNAP25<br />		[[5lob]], [[5low]] – rRab C2B domain + SNAP25<br />

Michal Harel at 09:49, 9 February 2022

Michal Harel — Wed, 09 Feb 2022 09:49:54 GMT

←Older revision		Revision as of 09:49, 9 February 2022
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	[[1zbd]] – rRab 19-217 (mutant) + rRab effector domain 40-179 (mutant) + GTP<br />		[[1zbd]] – rRab 19-217 (mutant) + rRab effector domain 40-179 (mutant) + GTP<br />
	[[2k3h]] – mRab C2A domain - mouse - NMR<br />		[[2k3h]] – mRab C2A domain - mouse - NMR<br />
-
-

	== References ==		== References ==

Alexander Berchansky at 12:39, 9 February 2021

Alexander Berchansky — Tue, 09 Feb 2021 12:39:33 GMT

←Older revision		Revision as of 12:39, 9 February 2021
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	== Structural highlights ==		== Structural highlights ==

-	<scene name='87/873795/Cv/2'>The 3D structure of the complex between three Rab C2B domains and a 4-helix bundle belonging to 4 SNAP-25 monomers</scene>. Two different <scene name='87/873795/Cv/5'>helix-helix interactions between Rab and SNAP-25</scene> are seen. One involves 2 monomers of SNAP-25 and the other involves one monomer<ref>PMID:28634303</ref>. Rab chain A interacts only with SNAP-25 chain D, while Rab chain B interacts with 2 SNAP-25 chains D (residues that interact with Rab chain B are colored salmon) and F.	+	<scene name='87/873795/Cv/2'>The 3D structure of the complex between three Rab C2B domains and a 4-helix bundle belonging to 4 SNAP-25 monomers</scene>. Two different <scene name='87/873795/Cv/6'>helix-helix interactions between Rab and SNAP-25</scene> are seen. One involves 2 monomers of SNAP-25 and the other involves one monomer<ref>PMID:28634303</ref>. Rab chain A interacts only with SNAP-25 chain D, while Rab chain B interacts with 2 SNAP-25 chains D (residues that interact with Rab chain B are colored salmon) and F.

	</StructureSection>		</StructureSection>

Alexander Berchansky at 12:36, 9 February 2021

Alexander Berchansky — Tue, 09 Feb 2021 12:36:13 GMT

←Older revision		Revision as of 12:36, 9 February 2021
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	== Structural highlights ==		== Structural highlights ==

-	<scene name='87/873795/Cv/2'>The 3D structure of the complex between three Rab C2B domains and a 4-helix bundle belonging to 4 SNAP-25 monomers</scene>. Two different helix-helix interactions between Rab and SNAP-25 are seen. One involves 2 monomers of SNAP-25 and the other involves one monomer<ref>PMID:28634303</ref>.	+	<scene name='87/873795/Cv/2'>The 3D structure of the complex between three Rab C2B domains and a 4-helix bundle belonging to 4 SNAP-25 monomers</scene>. Two different <scene name='87/873795/Cv/5'>helix-helix interactions between Rab and SNAP-25</scene> are seen. One involves 2 monomers of SNAP-25 and the other involves one monomer<ref>PMID:28634303</ref>. Rab chain A interacts only with SNAP-25 chain D, while Rab chain B interacts with 2 SNAP-25 chains D (residues that interact with Rab chain B are colored salmon) and F.

	</StructureSection>		</StructureSection>

Alexander Berchansky at 12:09, 9 February 2021

Alexander Berchansky — Tue, 09 Feb 2021 12:09:30 GMT

←Older revision		Revision as of 12:09, 9 February 2021
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	== Structural highlights ==		== Structural highlights ==

-	The 3D structure of the complex between three Rab C2B domains and a 4-helix bundle belonging to ~~two~~ SNAP-25 monomers. Two different helix-helix interactions between Rab and SNAP-25 are seen. One involves 2 monomers of SNAP-25 and the other involves one monomer<ref>PMID:28634303</ref>.	+	<scene name='87/873795/Cv/2'>The 3D structure of the complex between three Rab C2B domains and a 4-helix bundle belonging to 4 SNAP-25 monomers</scene>. Two different helix-helix interactions between Rab and SNAP-25 are seen. One involves 2 monomers of SNAP-25 and the other involves one monomer<ref>PMID:28634303</ref>.

	</StructureSection>		</StructureSection>

Alexander Berchansky at 13:05, 4 February 2021

Alexander Berchansky — Thu, 04 Feb 2021 13:05:37 GMT

←Older revision		Revision as of 13:05, 4 February 2021
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-	<StructureSection load='5lob' size='340' side='right' caption='Structure of rabphilin-3A C2B domain (green, grey, pink) complex with SNAP-25 N-terminal helix (yellow, magenta), glycerol, sulfate and Ca+2 ions (PDB code [[5lob]])' scene=''>	+	<StructureSection load='5lob' size='340' side='right' caption='Structure of rabphilin-3A C2B domain (green, grey, pink) complex with SNAP-25 N-terminal helix (yellow, magenta), glycerol, sulfate and Ca+2 ions (PDB code [[5lob]])' scene='87/873795/Cv/1'>

	== Function ==		== Function ==

Michal Harel at 10:06, 4 February 2021

Michal Harel — Thu, 04 Feb 2021 10:06:44 GMT

←Older revision		Revision as of 10:06, 4 February 2021
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-	<StructureSection load='5lob' size='340' side='right' caption='Structure of ~~glycosylated California poppy reticuline oxidase~~ complex with ~~reticuline~~ and ~~FAD~~ (PDB code [[5lob]])' scene=''>	+	<StructureSection load='5lob' size='340' side='right' caption='Structure of rabphilin-3A C2B domain (green, grey, pink) complex with SNAP-25 N-terminal helix (yellow, magenta), glycerol, sulfate and Ca+2 ions (PDB code [[5lob]])' scene=''>

	== Function ==		== Function ==
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	== Structural highlights ==		== Structural highlights ==

-	The 3D structure of the complex between RO and ~~its substrate reticuline show the cofactor FAD having bicovalent attachment~~ to ~~RO His~~ and ~~Cys~~. ~~The substrate reticuline is seen in the active site cavity interacting with FAD~~ and ~~numerous RO sidechains~~<ref>PMID:~~18953357~~</ref>.	+	The 3D structure of the complex between three Rab C2B domains and a 4-helix bundle belonging to two SNAP-25 monomers. Two different helix-helix interactions between Rab and SNAP-25 are seen. One involves 2 monomers of SNAP-25 and the other involves one monomer<ref>PMID:28634303</ref>.

	</StructureSection>		</StructureSection>

Michal Harel at 09:29, 4 February 2021

Michal Harel — Thu, 04 Feb 2021 09:29:16 GMT

←Older revision		Revision as of 09:29, 4 February 2021
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	== Function ==		== Function ==

-	'''Rabphilin~~''' or '''berberine bridge-forming enzyme~~''' (RO) is ~~part of the alkaloids biosynthesis pathway catalyzing the conversion of (S)~~-~~reticuline to (S)-scoulerine<ref>PMID:1946465</ref>. RO catalyzes~~ the ~~cyclization~~ of ~~the N-methyl moiety of (S)-reticuline into the berberine bridge carbon, C-8 of (S)~~- <ref>PMID:~~7592663~~</ref>.	+	'''Rabphilin''' (Rab) is known as a synaptic vesicle-associated protein involved in the regulation of exo- and endocytosis processes at presynaptic sites<ref>PMID:26679993</ref>.

	== Relevance ==		== Relevance ==

-	~~Reticuline~~ is a ~~precursor~~ of ~~morphine and has potent central nervous system depressing effects~~<ref>PMID:~~9720612~~</ref>.	+	Rab is a major auto antigen in lymphocytic infundibulo-neurohypophysitis (LINH). Antibodies to Rab may serve as a biomarker for the diagnosis of LINH<ref>PMID:25919460</ref>.

	== Structural highlights ==		== Structural highlights ==

Michal Harel at 08:00, 4 February 2021

Michal Harel — Thu, 04 Feb 2021 08:00:26 GMT

←Older revision		Revision as of 08:00, 4 February 2021
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-	<StructureSection load='~~3d2d~~' size='340' side='right' caption='Structure of glycosylated California poppy reticuline oxidase complex with reticuline and FAD (PDB code [[~~3d2d~~]])' scene='~~87/873793/Cv/1~~'>	+	<StructureSection load='5lob' size='340' side='right' caption='Structure of glycosylated California poppy reticuline oxidase complex with reticuline and FAD (PDB code [[5lob]])' scene=''>

	== Function ==		== Function ==
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	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}		Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}

-	[[2cm5]], [[2cm6]] – rRab C2B domain ~~541-680~~ – rat<br />	+	Domains: C2A 378-510; C2B 541-680
		+
		+	[[2cm5]], [[2cm6]] – rRab C2B domain – rat<br />
	[[4izk]] – rRab C2B domain - NMR<br />		[[4izk]] – rRab C2B domain - NMR<br />
	[[5lo8]] – rRab C2B domain + inositol phosphate derivative<br />		[[5lo8]] – rRab C2B domain + inositol phosphate derivative<br />
-	[[5lob]], [[5low]] – rRab ~~C2AB~~ domain + SNAP25<br />	+	[[5lob]], [[5low]] – rRab C2B domain + SNAP25<br />
-	[[2chd]], [[4lt7]] – rRab C2A domain ~~378-510~~ <br />	+	[[2chd]], [[4lt7]] – rRab C2A domain <br />
	[[4np9]], [[4ns0]] – rRab C2A domain + inositol phosphate derivative<br />		[[4np9]], [[4ns0]] – rRab C2A domain + inositol phosphate derivative<br />
	[[1zbd]] – rRab 19-217 (mutant) + rRab effector domain 40-179 (mutant) + GTP<br />		[[1zbd]] – rRab 19-217 (mutant) + rRab effector domain 40-179 (mutant) + GTP<br />
-	[[2k3h]] – mRab C2A domain ~~378-510~~ - mouse - NMR<br />	+	[[2k3h]] – mRab C2A domain - mouse - NMR<br />

Joel L. Sussman at 13:38, 3 February 2021

Joel L. Sussman — Wed, 03 Feb 2021 13:38:32 GMT

←Older revision		Revision as of 13:38, 3 February 2021
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	[[2cm5]], [[2cm6]] – rRab C2B domain 541-680 – rat<br />		[[2cm5]], [[2cm6]] – rRab C2B domain 541-680 – rat<br />
-	[[~~3rbp~~]] – rRab C2B domain - NMR<br />	+	[[4izk]] – rRab C2B domain - NMR<br />
	[[5lo8]] – rRab C2B domain + inositol phosphate derivative<br />		[[5lo8]] – rRab C2B domain + inositol phosphate derivative<br />
	[[5lob]], [[5low]] – rRab C2AB domain + SNAP25<br />		[[5lob]], [[5low]] – rRab C2AB domain + SNAP25<br />