Sandbox Reserved 1586 - Revision history

Anna Dickinson at 17:29, 20 November 2019

Anna Dickinson — Wed, 20 Nov 2019 17:29:11 GMT

←Older revision		Revision as of 17:29, 20 November 2019
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	== Structural Highlights ==		== Structural Highlights ==
-	Leadzyme, unlike other catalyic RNAs, can differ in sequences that are compatible with catalysis <ref name=red/>. This is due to only three bases being required for catalysis to occur: <scene name='82/824631/Important/1'> C6, G9, and G25 </scene>. <ref name= red/> Another important structural difference between leadzyme and other catalytic RNAs is a <scene name='82/824631/C3_pucker/2'>C3'-endo pucker</scene> at the cleavage site. This pucker increases the catalytic rate of leadzyme.	+	Leadzyme, unlike other catalyic RNAs, can differ in sequences that are compatible with catalysis <ref name=red/>. This is due to only three bases being required for catalysis to occur: <scene name='82/824631/Important/1'> C6, G9, and G25 </scene>. <ref name= red/> Another important structural difference between leadzyme and other catalytic RNAs is a <scene name='82/824631/C3_pucker/3'>C3'-endo pucker</scene> at the cleavage site. This pucker increases the catalytic rate of leadzyme.

	==References==		==References==
	<references />		<references />

Anna Dickinson at 17:26, 20 November 2019

Anna Dickinson — Wed, 20 Nov 2019 17:26:42 GMT

←Older revision		Revision as of 17:26, 20 November 2019
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	== Structural Highlights ==		== Structural Highlights ==
-	Leadzyme, unlike other catalyic RNAs, can differ in sequences that are compatible with catalysis <ref name=red/>. This is due to only three bases being required for catalysis to occur: <scene name='82/824631/Important/1'> C6, G9, and G25 </scene>. <ref name= red/> Another important structural difference between leadzyme and other catalytic RNAs is a <scene name='82/824631/C3_pucker/1'>C3'-endo pucker</scene> at the cleavage site. This pucker increases the catalytic rate of leadzyme.	+	Leadzyme, unlike other catalyic RNAs, can differ in sequences that are compatible with catalysis <ref name=red/>. This is due to only three bases being required for catalysis to occur: <scene name='82/824631/Important/1'> C6, G9, and G25 </scene>. <ref name= red/> Another important structural difference between leadzyme and other catalytic RNAs is a <scene name='82/824631/C3_pucker/2'>C3'-endo pucker</scene> at the cleavage site. This pucker increases the catalytic rate of leadzyme.

	==References==		==References==
	<references />		<references />

Anna Dickinson at 17:22, 20 November 2019

Anna Dickinson — Wed, 20 Nov 2019 17:22:40 GMT

←Older revision		Revision as of 17:22, 20 November 2019
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	{{Sandbox_Reserved_JMeans}}<!-- PLEASE ADD YOUR CONTENT BELOW HERE -->		{{Sandbox_Reserved_JMeans}}<!-- PLEASE ADD YOUR CONTENT BELOW HERE -->
	==Leadzyme==		==Leadzyme==
-	<StructureSection load='2LDZ' size='400' ~~side~~='~~left~~' caption='Leadzyme Structure' scene=''/>	+	<StructureSection load='2LDZ' size='400' align='right' caption='Leadzyme Structure' scene=''/>

	== Importance in Health ==		== Importance in Health ==

Anna Dickinson at 17:21, 20 November 2019

Anna Dickinson — Wed, 20 Nov 2019 17:21:49 GMT

←Older revision		Revision as of 17:21, 20 November 2019
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	{{Sandbox_Reserved_JMeans}}<!-- PLEASE ADD YOUR CONTENT BELOW HERE -->		{{Sandbox_Reserved_JMeans}}<!-- PLEASE ADD YOUR CONTENT BELOW HERE -->
	==Leadzyme==		==Leadzyme==
-	<StructureSection load='2LDZ' size='400' side='~~right~~' caption='Leadzyme Structure' scene=''/>	+	<StructureSection load='2LDZ' size='400' side='left' caption='Leadzyme Structure' scene=''/>

	== Importance in Health ==		== Importance in Health ==

Anna Dickinson at 17:21, 20 November 2019

Anna Dickinson — Wed, 20 Nov 2019 17:21:29 GMT

←Older revision		Revision as of 17:21, 20 November 2019
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	{{Sandbox_Reserved_JMeans}}<!-- PLEASE ADD YOUR CONTENT BELOW HERE -->		{{Sandbox_Reserved_JMeans}}<!-- PLEASE ADD YOUR CONTENT BELOW HERE -->
	==Leadzyme==		==Leadzyme==
-	<StructureSection load='2LDZ' size='400' ~~frame='true' align~~='right' caption='Leadzyme Structure' scene='~~Leadzyme~~'/>	+	<StructureSection load='2LDZ' size='400' side='right' caption='Leadzyme Structure' scene=''/>

	== Importance in Health ==		== Importance in Health ==

Anna Dickinson at 17:19, 20 November 2019

Anna Dickinson — Wed, 20 Nov 2019 17:19:41 GMT

←Older revision		Revision as of 17:19, 20 November 2019
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	{{Sandbox_Reserved_JMeans}}<!-- PLEASE ADD YOUR CONTENT BELOW HERE -->		{{Sandbox_Reserved_JMeans}}<!-- PLEASE ADD YOUR CONTENT BELOW HERE -->
	==Leadzyme==		==Leadzyme==
-	<StructureSection load='2LDZ' size='400' frame='true' align='right' caption='Leadzyme Structure' scene='Leadzyme'>	+	<StructureSection load='2LDZ' size='400' frame='true' align='right' caption='Leadzyme Structure' scene='Leadzyme'/>

	== Importance in Health ==		== Importance in Health ==

Anna Dickinson at 17:19, 20 November 2019

Anna Dickinson — Wed, 20 Nov 2019 17:19:31 GMT

←Older revision		Revision as of 17:19, 20 November 2019
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	{{Sandbox_Reserved_JMeans}}<!-- PLEASE ADD YOUR CONTENT BELOW HERE -->		{{Sandbox_Reserved_JMeans}}<!-- PLEASE ADD YOUR CONTENT BELOW HERE -->
-	==Leadzyme ~~Structure~~==	+	==Leadzyme==
-	<StructureSection load='2LDZ' size='400' frame='true' align='right' caption='Leadzyme Structure' scene='Leadzyme'/>	+	<StructureSection load='2LDZ' size='400' frame='true' align='right' caption='Leadzyme Structure' scene='Leadzyme'>
-		+
-	~~<scene name='82/824631/Stereoview/1'>Leadzyme</scene~~>	+

	== Importance in Health ==		== Importance in Health ==

Anna Dickinson at 17:17, 20 November 2019

Anna Dickinson — Wed, 20 Nov 2019 17:17:09 GMT

←Older revision		Revision as of 17:17, 20 November 2019
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	==Leadzyme Structure==		==Leadzyme Structure==
	<StructureSection load='2LDZ' size='400' frame='true' align='right' caption='Leadzyme Structure' scene='Leadzyme'/>		<StructureSection load='2LDZ' size='400' frame='true' align='right' caption='Leadzyme Structure' scene='Leadzyme'/>
-	~~<scene name='82/824631/C3_pucker/1'>TextToBeDisplayed</scene>~~	+
	<scene name='82/824631/Stereoview/1'>Leadzyme</scene>		<scene name='82/824631/Stereoview/1'>Leadzyme</scene>

	== Importance in Health ==		== Importance in Health ==
-	Lead is an extremely toxic agent and is a commonly used heavy metal. In humans, over exposure to lead raises the levels of lead in the biological system and has been linked to numerous health disorders <ref name= "green">PMID:15795164</ref> . The effects of lead exposure can affect reproductive systems, nervous system, and carcinogenicity <ref name= "purple">PMID:28731299</ref> . However, the pathway in which lead affects the body in each case is not known. Therefore, the discovery of leadzyme allows the lead-induced disorders to be further understood.	+	Lead is an extremely toxic agent and is a commonly used heavy metal. In humans, over exposure to lead raises the levels of lead in the biological system and has been linked to numerous health disorders. <ref name= "green">PMID:15795164</ref> The effects of lead exposure can affect reproductive systems, nervous system, and carcinogenicity. <ref name= "purple">PMID:28731299</ref> However, the pathway in which lead affects the body in each case is not known. Therefore, the discovery of leadzyme allows the lead-induced disorders to be further understood.

	== History ==		== History ==
-	<scene name='82/824631/Lead_dependent_ribozyme/1'>Leadzyme</scene> was discovered by Uhlenbeck and co-worked in 1992, while they were searching for RNAs that cleaved in the presence of lead <ref name="red">PMID:9813122</ref>. The discovery was by ''in vitro'' selection, which allows for an isolation and amplification of selected functional molecules. This method has been key in the discovery of numerous RNA and DNA catalysis. Leadzyme, or lead-dependent ribozyme, is among the smallest known catalytic RNAs <ref name=red/>.	+	<scene name='82/824631/Lead_dependent_ribozyme/1'>Leadzyme</scene> was discovered by Uhlenbeck and co-worked in 1992, while they were searching for RNAs that cleaved in the presence of lead. <ref name="red">PMID:9813122</ref> The discovery was by ''in vitro'' selection, which allows for an isolation and amplification of selected functional molecules. This method has been key in the discovery of numerous RNA and DNA catalysis. Leadzyme, or lead-dependent ribozyme, is among the smallest known catalytic RNAs. <ref name=red/>

	== Function ==		== Function ==
-	Leadzyme is a relatively small catalytic RNA that uses a unique two-step reaction to cleave within the active site <ref name= "blue">PMID:25962038</ref>.	+	Leadzyme is a relatively small catalytic RNA that uses a unique two-step reaction to cleave within the active site. <ref name= "blue">PMID:25962038</ref>
-	In the presence of Pb^2+, it catalyzes the cleavage of the C6-C7 phosphodiester bond <ref name= red/> . This is accomplished via nucleophilic attack <ref name=red/>. The resulting in a 2', 3'-cyclic phosphate and a 5'-hydroxyl terminus <ref name= red/>. The nuclear magnetic resonance spectrospcopy (NMR) and crystal structure of leadzyme has been used to try to better understand the cleavage reaction process <ref name=red/>. However, neither data sets revealed the proposed in-line alignment for attack by the 2'-OH nucleophilic group as described experimentally. <ref name=blue/>	+	In the presence of Pb^2+, it catalyzes the cleavage of the C6-C7 phosphodiester bond. <ref name= red/> This is accomplished via nucleophilic attack. <ref name=red/> The resulting in a 2', 3'-cyclic phosphate and a 5'-hydroxyl terminus. <ref name= red/> The nuclear magnetic resonance spectrospcopy (NMR) and crystal structure of leadzyme has been used to try to better understand the cleavage reaction process. <ref name=red/> However, neither data sets revealed the proposed in-line alignment for attack by the 2'-OH nucleophilic group as described experimentally. <ref name=blue/> Therefore: it is hypothesized that the structures represent ground states of leadzyme and are not catalytically active. <ref name=blue/>


	== Structural Highlights ==		== Structural Highlights ==
-	~~Therefore: it is hypothesized that the structures represent ground states of leadzyme and are not catalytically active <ref name=blue/>.~~ Leadzyme, unlike other catalyic RNAs, can differ in sequences that are compatible with catalysis <ref name=red/>. This is due to only three bases being required for catalysis to occur: <scene name='82/824631/Important/1'> C6, G9, and G25 </scene>.	+	Leadzyme, unlike other catalyic RNAs, can differ in sequences that are compatible with catalysis <ref name=red/>. This is due to only three bases being required for catalysis to occur: <scene name='82/824631/Important/1'> C6, G9, and G25 </scene>. <ref name= red/> Another important structural difference between leadzyme and other catalytic RNAs is a <scene name='82/824631/C3_pucker/1'>C3'-endo pucker</scene> at the cleavage site. This pucker increases the catalytic rate of leadzyme.

	==References==		==References==
-
	<references />		<references />

Anna Dickinson at 17:08, 20 November 2019

Anna Dickinson — Wed, 20 Nov 2019 17:08:37 GMT

←Older revision		Revision as of 17:08, 20 November 2019
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	== Function ==		== Function ==
	Leadzyme is a relatively small catalytic RNA that uses a unique two-step reaction to cleave within the active site <ref name= "blue">PMID:25962038</ref>.		Leadzyme is a relatively small catalytic RNA that uses a unique two-step reaction to cleave within the active site <ref name= "blue">PMID:25962038</ref>.
-	In the presence of Pb^2+, it catalyzes the cleavage of the C6-C7 phosphodiester bond <ref name= red/> . This is accomplished via nucleophilic attack <ref name=red/>. The resulting in a 2', 3'-cyclic phosphate and a 5'-hydroxyl terminus <ref name= red/>. The nuclear magnetic resonance spectrospcopy (NMR) and crystal structure of leadzyme has been used to try to better understand the cleavage reaction process <ref name=red/>. However, neither data sets revealed the proposed in-line alignment for attack by the 2'-OH nucleophilic group as described experimentally <ref name=blue>. Therefore: it is hypothesized that the structures represent ground states of leadzyme and are not catalytically active <ref name=blue/>. Leadzyme, unlike other catalyic RNAs, can differ in sequences that are compatible with catalysis <ref name=red/>. This is due to only three bases being required for catalysis to occur: <scene name='82/824631/Important/1'> C6, G9, and G25 </scene>.	+	In the presence of Pb^2+, it catalyzes the cleavage of the C6-C7 phosphodiester bond <ref name= red/> . This is accomplished via nucleophilic attack <ref name=red/>. The resulting in a 2', 3'-cyclic phosphate and a 5'-hydroxyl terminus <ref name= red/>. The nuclear magnetic resonance spectrospcopy (NMR) and crystal structure of leadzyme has been used to try to better understand the cleavage reaction process <ref name=red/>. However, neither data sets revealed the proposed in-line alignment for attack by the 2'-OH nucleophilic group as described experimentally. <ref name=blue/>
		+
		+
		+	== Structural Highlights ==
		+	Therefore: it is hypothesized that the structures represent ground states of leadzyme and are not catalytically active <ref name=blue/>. Leadzyme, unlike other catalyic RNAs, can differ in sequences that are compatible with catalysis <ref name=red/>. This is due to only three bases being required for catalysis to occur: <scene name='82/824631/Important/1'> C6, G9, and G25 </scene>.

	==References==		==References==

	<references />		<references />

Anna Dickinson at 17:02, 20 November 2019

Anna Dickinson — Wed, 20 Nov 2019 17:02:19 GMT

←Older revision		Revision as of 17:02, 20 November 2019
Line 15:		Line 15:
	In the presence of Pb^2+, it catalyzes the cleavage of the C6-C7 phosphodiester bond <ref name= red/> . This is accomplished via nucleophilic attack <ref name=red/>. The resulting in a 2', 3'-cyclic phosphate and a 5'-hydroxyl terminus <ref name= red/>. The nuclear magnetic resonance spectrospcopy (NMR) and crystal structure of leadzyme has been used to try to better understand the cleavage reaction process <ref name=red/>. However, neither data sets revealed the proposed in-line alignment for attack by the 2'-OH nucleophilic group as described experimentally <ref name=blue>. Therefore: it is hypothesized that the structures represent ground states of leadzyme and are not catalytically active <ref name=blue/>. Leadzyme, unlike other catalyic RNAs, can differ in sequences that are compatible with catalysis <ref name=red/>. This is due to only three bases being required for catalysis to occur: <scene name='82/824631/Important/1'> C6, G9, and G25 </scene>.		In the presence of Pb^2+, it catalyzes the cleavage of the C6-C7 phosphodiester bond <ref name= red/> . This is accomplished via nucleophilic attack <ref name=red/>. The resulting in a 2', 3'-cyclic phosphate and a 5'-hydroxyl terminus <ref name= red/>. The nuclear magnetic resonance spectrospcopy (NMR) and crystal structure of leadzyme has been used to try to better understand the cleavage reaction process <ref name=red/>. However, neither data sets revealed the proposed in-line alignment for attack by the 2'-OH nucleophilic group as described experimentally <ref name=blue>. Therefore: it is hypothesized that the structures represent ground states of leadzyme and are not catalytically active <ref name=blue/>. Leadzyme, unlike other catalyic RNAs, can differ in sequences that are compatible with catalysis <ref name=red/>. This is due to only three bases being required for catalysis to occur: <scene name='82/824631/Important/1'> C6, G9, and G25 </scene>.

-	== References ==	+	==References==
		+
	<references />		<references />