Sigma factor - Revision history

Michal Harel at 10:03, 1 April 2020

Michal Harel — Wed, 01 Apr 2020 10:03:40 GMT

←Older revision		Revision as of 10:03, 1 April 2020
Line 3:		Line 3:
	== Overview ==		== Overview ==

-	'''Sigma (σ) factor''' is the peoptide subunit needed for the initiation of RNA transcription in prokaryotic organisms <scene name='59/591940/Sigma_factor_in_enzyme/1'>as seen here</scene>. As opposed to eukaryotes, who utilize a variety of proteins to initiate gene transcription, prokaryotic transcription is initiated almost completely by a σ-factor. The large and biologically essential protein, RNA polymerase (RNAP), contains one σ-subunit, which binds <scene name='59/591940/Dna_promoter/3'>DNA promoter sequences</scene>, located upstream of transcription start sites.	+	'''Sigma (σ) factor''' is the peoptide subunit needed for the initiation of RNA transcription in prokaryotic organisms <scene name='59/591940/Sigma_factor_in_enzyme/1'>as seen here</scene>. As opposed to eukaryotes, who utilize a variety of proteins to initiate gene transcription, prokaryotic transcription is initiated almost completely by a σ-factor. The large and biologically essential protein, RNA polymerase (RNAP), contains one σ-subunit, which binds <scene name='59/591940/Dna_promoter/3'>DNA promoter sequences</scene>, located upstream of transcription start sites. <br />
		+	*'''Sigma factor 70''' or '''RpoD''' is the primary initiation factor during exponential growth. <br />
		+	*'''Sigma factor 45''' or '''RpoN''' is responsible for the expression of genes involved in Arg catabolism.<br />
		+	*'''Sigma factor 38''' or '''RpoS''' acts as the master regulator of the general stress response in ''E. coli'' .<br />

	== Function and Structure ==		== Function and Structure ==

Michal Harel at 08:15, 6 February 2020

Michal Harel — Thu, 06 Feb 2020 08:15:02 GMT

←Older revision		Revision as of 08:15, 6 February 2020
Line 34:		Line 34:
	</StructureSection>		</StructureSection>

-	==3D structures of sigma factor==
-
-	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
-	{{#tree:id=OrganizedByTopic\|openlevels=0\|
-
-	*Sigma factor
-
-	**[[1ku2]] – TaSF region 1.2-3.1 – ''Thermus aquaticus''<br />
-	**[[1ku3]] – TaSF region 4 (mutant)<br />
-	**[[3les]] – TaSF residues 93-271<br />
-	**[[1sig]] – EcSF – ''Escherichia coli''<br />
-	**[[2mao]] – EcSF region 2 - NMR<br />
-	**[[1tty]] – TmSF-70 region 4 – ''Thermotoga maritima''<br />
-	**[[2k6x]] – TmSF-70 region 1.1 (mutant) - NMR<br />
-	**[[2ahq]] – AaSF-54 C terminal – ''Aquifex aeolicus'' - NMR<br />
-	**[[2k9l]], [[2k9m]] – AaSF-54 core domain - NMR<br />
-	**[[3mzy]] – SF-H – ''Fusobacterium nucleatum''<br />
-	**[[1h3l]] – ScSF N terminal – ''Streptomyces coelicolor''<br />
-	**[[5fgm]] – ScSF-R region 4<br />
-	**[[5mww]] – BsSF-A region 1.1 - ''Bacillus subtilis'' - NMR <br />
-	**[[5xe7]] – MtSF-J – ''Mycobacterium tuberculosis''<br />
-
-	*Sigma factor complex with DNA
-
-	**[[3ugo]], [[3ugp]] - TaSF region 2 + DNA<br />
-	**[[4ki2]] - TaSF region 2-3 + DNA<br />
-	**[[2h27]] – EcSF-E region 4 + DNA<br />
-	**[[2map]] – EcSF region 2 + DNA - NMR<br />
-	**[[1ku7]] – EcSF region 4 (mutant) + DNA <br />
-	**[[2o8k]], [[2o9l]] – AaSF-54 C terminal + DNA - NMR<br />
-	**[[5ui5]] – AaSF RpoN residues 61-398 + DNA <br />
-
-	*Sigma factor complex with protein
-
-	**[[3lev]] – TaSF residues 93-271 + antibody<br />
-	**[[1l9u]] – TaSF region 1.1-4 + RNAP subunits α,β,β’,ω<br />
-	**[[1or7]] – EcSF-E + σ-E factor negative regulatory protein <br />
-	**[[4lup]] – EcSF residues 3-92 + EcSF region 2 <br />
-	**[[1tlh]] – EcSF ProD region 4 + anti-σ factor<br />
-	**[[2p7v]] – EcSF ProD region 4 + regulator of σ D<br />
-	**[[4yg2]], [[5uag]], [[5uaj]], [[5uaq]] – EcSF ProD + RNAP subunits α,β,β’,ω <br />
-	**[[5ui8]] – EcSF-45 + RNAP subunits α,β,β’,ω <br />
-	**[[4mex]], [[4kmu]], [[4kn4]], [[4kn7]], [[5uac]], [[5uah]], [[5ual]], [[4zh3]], [[4zh4]] – EcSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />
-	**[[4xsx]], [[4xsy]], [[4xsz]] – EcSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />
-	**[[6byu]] – EcSF ProD + RNAP subunits α,β,β’,ω + ppApp <br />
-	**[[4mey]], [[4ljz]], [[4lk1]], [[6cux]] – EcSF ProD + RNAP subunits α,β,β’,ω <br />
-	**[[6c9y]], [[6ca0]] – EcSF ProD + RNAP subunits α,β,β’,ω - Cryo EM<br />
-	**[[5byh]] – EcSF-45 + RNAP subunits α,β,β’,ω <br />
-	**[[5nwt]] – EcSF-54 + RNAP subunits α,β,β’,ω <br />
-	**[[5w1s]] – EcSF RpoD + RNAP subunits α,β,β’ + TraR<br />
-	**[[5vsw]] – EcSF RpoD + RNAP subunits α,β,β’ + DnaK suppressor protein<br />
-	**[[5w1t]] – EcSF RpoD + RNAP subunits α,β,β’ + RpoZ + DnaK suppressor protein<br />
-	**[[4lk0]], [[4llg]], [[4yfk]], [[4yfn]], [[4yfx]], [[4zh2]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + RNAP inhibitor<br />
-	**[[1rp3]], [[1sc5]] – AaSF-28 + anti-σ factor FLGM <br />
-	**[[5wuq]] – BsSF SIGW + anti-σ factor RSIW <br />
-	**[[3hug]] – MtSF-L + membrane protein <br />
-	**[[4nqw]] – MtSF-K region 4 + anti-σ factor<br />
-	**[[4x8k]] – MtSF-A region 2 + RBPA<br />
-	**[[6fbv]] – MtSF-A + RNAP subunits α,β,β’,ω - SP Cryo EM<br />
-	**[[6c05]], [[6c06]] – MtSF-A + RNAP subunits α,β,β’,ω + RbpA - Cryo EM<br />
-	**[[3wod]], [[2a6e]], [[2cw0]], [[5tmf]] – TtSF + RNAP subunits α,β,β’,ω - ''Thermus thermophilus'' <br />
-	**[[4mq9]], [[2a68]], [[2a69]], [[2a6h]], [[3dxj]], [[3eql]] – TtSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />
-	**[[2be5]] – TtSF + RNAP subunits α,β,β’,ω + inhibitor<br />
-	**[[5tmc]] – TtSF-A + RNAP subunits α,β,β’,ω + protein + G4P<br />
-	**[[5xj0]] – TtSF-A + RNAP subunits α,β,β’,ω + GP39 + GP76<br />
-	**[[5x22]] – TtSF-A + RNAP subunits α,β,β’,ω + GpA + CMPcPP<br />
-	**[[4lk0]], [[4llg]], [[4yfk]], [[4yfn]], [[4yfx]], [[4zh2]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + RNAP inhibitor<br />
-	**[[4cxf]] – SF CNRH + CNRY – ''Cupriavidus metallidurans''<br />
-	**[[4g6d]], [[4g8x]], [[4g94]] – SF ProD region 4 + Orf067 – ''Staphylococcus aureus''<br />
-	**[[5yix]] – SF RpoD + GCRA - ''Caulobacter crescentus''<br />
-
-	*Sigma factor complex with protein and DNA
-
-	**[[4oio]], [[4g7h]], [[4g7o]], [[4g7z]], [[4q4z]], [[4q5s]] – TtSF SIGA + RNAP subunits α,β,β’,ω + DNA<br />
-	**[[1smy]] – TtSF + RNAP subunits α,β,β’,ω + G4P<br />
-	**[[3n97]] – TaSF region 4 + RNAP subunits α + DNA<br />
-	**[[4xlp]], [[4xlq]], [[5tjg]] – TaSF SIGA + RNAP subunits α,β,β’,ω + DNA <br />
-	**[[1l9z]] – TaSF + RNAP subunits α,β,β’,ω + DNA – Cryo EM <br />
-	**[[4xlr]], [[4xls]] – TaSF-A + RNAP subunits α,β,β’,ω + CarD-like transcriptional regulator + DNA <br />
-	**[[1rio]] - TaSF region 4 + repression protein CI + DNA<br />
-	**[[3iyd]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + catabolite gene activator + DNA<br />
-	**[[4jk1]], [[4jk2]], [[4jkr]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + G4P<br />
-	**[[4yln]], [[4ylo]], [[4ylp]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + DNA<br />
-	**[[5nss]] – EcSF-54 + EcRNAP subunits α,β,β’,ω + phage shock protein F + DNA - Cryo EM<br />
-	**[[5nsr]], [[6gh6]], [[6gh5]], [[6gfw]] – EcSF-54 + EcRNAP subunits α,β,β’,ω + DNA - Cryo EM<br />
-	**[[5voi]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA <br />
-	**[[5d4c]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + CTP + ATP<br />
-	**[[5d4d]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + CTP + NAD<br />
-	**[[5d4e]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + CTP + dephosphate-CoA<br />
-	**[[5uha]] – MtSF-A + MtRNAP subunits α,β,β’,ω + DNA <br />
-	**[[6c04]] – MtSF-A + RNAP subunits α,β,β’,ω + RbpA + DNA - Cryo EM<br />
-	**[[5uhe]], [[5uhf]] – MtSF SIGA + MtRNAP subunits α,β,β’,ω + DNA + inhibitor<br />
-	**[[5tw1]], [[6cce]] – MsSF-A + MtRNAP subunits α,β,β’,ω + DNA + peptide - ''Mycobacterium smegmatis''<br />
-	**[[5vi8]] – MsSF-A + MsRNAP subunits α,β,β’,ω + DNA <br />
-	**[[6ccv]] – MsSF-A + MtRNAP subunits α,β,β’,ω + DNA + peptide + RbpA <br />
-
-	*Sigma factor complex with protein and DNA and antibiotics
-
-	**[[4oin]], [[4oip]], [[4oiq]], [[4oir]] – TtSF-A + RNAP subunits α,β,β’,ω + antibiotic + DNA<br />
-	**[[6cuu]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + antibiotic<br />
-	**[[5uhg]] – MtSF-A + MtRNAP subunits α,β,β’,ω + DNA + antibiotic + inhibitor<br />
-	**[[5uhb]] – MtSF-A + MtRNAP subunits α,β,β’,ω + DNA + antibiotic<br />
-	**[[6bzo]] – MtSF-A + RNAP subunits α,β,β’,ω + RbpA + DNA + antibiotic - Cryo EM<br />
-	**[[6ccf]] – MsSF-A + MtRNAP subunits α,β,β’,ω + DNA + peptide + antibiotic<br />
-
-	*Sigma factor complex with protein, DNA and RNA
-
-	**[[5vi5]] – MsSF-A + MsRNAP subunits α,β,β’,ω + RbpA + DNA + RNA<br />
-	**[[4xln]] – TaSF-A + RNAP subunits α,β,β’,ω + DNA + RNA <br />
-	**[[5ipl]], [[5ipm]], [[5ipn]] – EcSF RpoS + EcRNAP subunits α,β,β’,ω + DNA + RNA<br />
-	**[[6b6h]] – EcSF RpoD + EcRNAP subunits α,β,β’,ω + DNA + RNA - Cryo EM<br />
-	**[[5e17]], [[5e18]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + RNA<br />
-	**[[5x22]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + RNA + CMPPP<br />
-	**[[5vo8]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + RNA + GTP<br />
-	**[[5x21]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + RNA + antibiotic<br />
-	**[[5i2d]] – TtSF-A + TtRNAP subunits α,β,β’,ω + transcriptional regulator + DNA + RNA<br />
-	**[[5uh5]], [[5uh8]], [[5uh9]] – MtSF-A + MtRNAP subunits α,β,β’,ω + DNA + RNA<br />
-	**[[5uh6]], [[5uhc]], [[5uhd]], [[5x21]] – MtSF-A + TtRNAP subunits α,β,β’,ω + DNA + RNA + antibiotic<br />
-	}}
	== References ==		== References ==

Michal Harel at 08:13, 6 February 2020

Michal Harel — Thu, 06 Feb 2020 08:13:42 GMT

←Older revision		Revision as of 08:13, 6 February 2020
Line 28:		Line 28:
	== Gene Regulation and Differentiation ==		== Gene Regulation and Differentiation ==
	Since σ-factors are exclusively linked to gene expression in prokaryotic organisms, the variety of σ-factors in a cell dictate how and what genes are transcribed. Specialized function in cells, therefore, is highly moderated by its arsenal of σ-subunits. In fact, cellular development and differentiation are directly impacted and carried out by "cascades" of σ-factors. In the early stages of development, '''early genes'''[2] are transcribed by basic '''bacterial σ-factors'''. These genes are therefore transcribed to give new σ-factors, which in turn activate additional genes, and so on [2]. This process of σ-factor cascades demonstrates the versatile and essential biologic functions of the RNAP subunit, σ.		Since σ-factors are exclusively linked to gene expression in prokaryotic organisms, the variety of σ-factors in a cell dictate how and what genes are transcribed. Specialized function in cells, therefore, is highly moderated by its arsenal of σ-subunits. In fact, cellular development and differentiation are directly impacted and carried out by "cascades" of σ-factors. In the early stages of development, '''early genes'''[2] are transcribed by basic '''bacterial σ-factors'''. These genes are therefore transcribed to give new σ-factors, which in turn activate additional genes, and so on [2]. This process of σ-factor cascades demonstrates the versatile and essential biologic functions of the RNAP subunit, σ.
		+
		+	==3D structures of sigma factor==
		+	[[Sigma factor 3D structures]]
		+
	</StructureSection>		</StructureSection>

Michal Harel at 10:21, 5 February 2020

Michal Harel — Wed, 05 Feb 2020 10:21:01 GMT

←Older revision		Revision as of 10:21, 5 February 2020
Line 65:		Line 65:

	**[[3lev]] – TaSF residues 93-271 + antibody<br />		**[[3lev]] – TaSF residues 93-271 + antibody<br />
		+	**[[1l9u]] – TaSF region 1.1-4 + RNAP subunits α,β,β’,ω<br />
	**[[1or7]] – EcSF-E + σ-E factor negative regulatory protein <br />		**[[1or7]] – EcSF-E + σ-E factor negative regulatory protein <br />
	**[[4lup]] – EcSF residues 3-92 + EcSF region 2 <br />		**[[4lup]] – EcSF residues 3-92 + EcSF region 2 <br />
	**[[1tlh]] – EcSF ProD region 4 + anti-σ factor<br />		**[[1tlh]] – EcSF ProD region 4 + anti-σ factor<br />
	**[[2p7v]] – EcSF ProD region 4 + regulator of σ D<br />		**[[2p7v]] – EcSF ProD region 4 + regulator of σ D<br />
		+	**[[4yg2]], [[5uag]], [[5uaj]], [[5uaq]] – EcSF ProD + RNAP subunits α,β,β’,ω <br />
		+	**[[5ui8]] – EcSF-45 + RNAP subunits α,β,β’,ω <br />
		+	**[[4mex]], [[4kmu]], [[4kn4]], [[4kn7]], [[5uac]], [[5uah]], [[5ual]], [[4zh3]], [[4zh4]] – EcSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />
		+	**[[4xsx]], [[4xsy]], [[4xsz]] – EcSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />
		+	**[[6byu]] – EcSF ProD + RNAP subunits α,β,β’,ω + ppApp <br />
		+	**[[4mey]], [[4ljz]], [[4lk1]], [[6cux]] – EcSF ProD + RNAP subunits α,β,β’,ω <br />
		+	**[[6c9y]], [[6ca0]] – EcSF ProD + RNAP subunits α,β,β’,ω - Cryo EM<br />
		+	**[[5byh]] – EcSF-45 + RNAP subunits α,β,β’,ω <br />
		+	**[[5nwt]] – EcSF-54 + RNAP subunits α,β,β’,ω <br />
		+	**[[5w1s]] – EcSF RpoD + RNAP subunits α,β,β’ + TraR<br />
		+	**[[5vsw]] – EcSF RpoD + RNAP subunits α,β,β’ + DnaK suppressor protein<br />
		+	**[[5w1t]] – EcSF RpoD + RNAP subunits α,β,β’ + RpoZ + DnaK suppressor protein<br />
		+	**[[4lk0]], [[4llg]], [[4yfk]], [[4yfn]], [[4yfx]], [[4zh2]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + RNAP inhibitor<br />
	**[[1rp3]], [[1sc5]] – AaSF-28 + anti-σ factor FLGM <br />		**[[1rp3]], [[1sc5]] – AaSF-28 + anti-σ factor FLGM <br />
	**[[5wuq]] – BsSF SIGW + anti-σ factor RSIW <br />		**[[5wuq]] – BsSF SIGW + anti-σ factor RSIW <br />
Line 82:		Line 96:
	**[[5xj0]] – TtSF-A + RNAP subunits α,β,β’,ω + GP39 + GP76<br />		**[[5xj0]] – TtSF-A + RNAP subunits α,β,β’,ω + GP39 + GP76<br />
	**[[5x22]] – TtSF-A + RNAP subunits α,β,β’,ω + GpA + CMPcPP<br />		**[[5x22]] – TtSF-A + RNAP subunits α,β,β’,ω + GpA + CMPcPP<br />
-	**[[1l9u]] – TaSF region 1.1-4 + RNAP subunits α,β,β’,ω<br />
-	**[[4yg2]], [[5uag]], [[5uaj]], [[5uaq]] – EcSF ProD + RNAP subunits α,β,β’,ω <br />
-	**[[5ui8]] – EcSF-45 + RNAP subunits α,β,β’,ω <br />
-	**[[4mex]], [[4kmu]], [[4kn4]], [[4kn7]], [[5uac]], [[5uah]], [[5ual]], [[4zh3]], [[4zh4]] – EcSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />
-	**[[4xsx]], [[4xsy]], [[4xsz]] – EcSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />
-	**[[6byu]] – EcSF ProD + RNAP subunits α,β,β’,ω + ppApp <br />
-	**[[4mey]], [[4ljz]], [[4lk1]], [[6cux]] – EcSF ProD + RNAP subunits α,β,β’,ω <br />
-	**[[6c9y]], [[6ca0]] – EcSF ProD + RNAP subunits α,β,β’,ω - Cryo EM<br />
-	**[[5byh]] – EcSF-45 + RNAP subunits α,β,β’,ω <br />
-	**[[5nwt]] – EcSF-54 + RNAP subunits α,β,β’,ω <br />
-	**[[5w1s]] – EcSF RpoD + RNAP subunits α,β,β’ + TraR<br />
-	**[[5vsw]] – EcSF RpoD + RNAP subunits α,β,β’ + DnaK suppressor protein<br />
-	**[[5w1t]] – EcSF RpoD + RNAP subunits α,β,β’ + RpoZ + DnaK suppressor protein<br />
	**[[4lk0]], [[4llg]], [[4yfk]], [[4yfn]], [[4yfx]], [[4zh2]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + RNAP inhibitor<br />		**[[4lk0]], [[4llg]], [[4yfk]], [[4yfn]], [[4yfx]], [[4zh2]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + RNAP inhibitor<br />
	**[[4cxf]] – SF CNRH + CNRY – ''Cupriavidus metallidurans''<br />		**[[4cxf]] – SF CNRH + CNRY – ''Cupriavidus metallidurans''<br />
Line 102:		Line 103:
	*Sigma factor complex with protein and DNA		*Sigma factor complex with protein and DNA

-	**[[1rio]] - TaSF region 4 + repression protein CI + DNA<br />
	**[[4oio]], [[4g7h]], [[4g7o]], [[4g7z]], [[4q4z]], [[4q5s]] – TtSF SIGA + RNAP subunits α,β,β’,ω + DNA<br />		**[[4oio]], [[4g7h]], [[4g7o]], [[4g7z]], [[4q4z]], [[4q5s]] – TtSF SIGA + RNAP subunits α,β,β’,ω + DNA<br />
	**[[1smy]] – TtSF + RNAP subunits α,β,β’,ω + G4P<br />		**[[1smy]] – TtSF + RNAP subunits α,β,β’,ω + G4P<br />
	**[[3n97]] – TaSF region 4 + RNAP subunits α + DNA<br />		**[[3n97]] – TaSF region 4 + RNAP subunits α + DNA<br />
	**[[4xlp]], [[4xlq]], [[5tjg]] – TaSF SIGA + RNAP subunits α,β,β’,ω + DNA <br />		**[[4xlp]], [[4xlq]], [[5tjg]] – TaSF SIGA + RNAP subunits α,β,β’,ω + DNA <br />
-	**~~[[1l9u]],~~ [[1l9z]] – TaSF + RNAP subunits α,β,β’,ω + DNA – Cryo EM <br />	+	**[[1l9z]] – TaSF + RNAP subunits α,β,β’,ω + DNA – Cryo EM <br />
	**[[4xlr]], [[4xls]] – TaSF-A + RNAP subunits α,β,β’,ω + CarD-like transcriptional regulator + DNA <br />		**[[4xlr]], [[4xls]] – TaSF-A + RNAP subunits α,β,β’,ω + CarD-like transcriptional regulator + DNA <br />
		+	**[[1rio]] - TaSF region 4 + repression protein CI + DNA<br />
	**[[3iyd]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + catabolite gene activator + DNA<br />		**[[3iyd]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + catabolite gene activator + DNA<br />
	**[[4jk1]], [[4jk2]], [[4jkr]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + G4P<br />		**[[4jk1]], [[4jk2]], [[4jkr]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + G4P<br />

Michal Harel at 22:58, 19 September 2018

Michal Harel — Wed, 19 Sep 2018 22:58:34 GMT

←Older revision		Revision as of 22:58, 19 September 2018
Line 103:		Line 103:

	**[[1rio]] - TaSF region 4 + repression protein CI + DNA<br />		**[[1rio]] - TaSF region 4 + repression protein CI + DNA<br />
-	**[[4oin]], [[4oip]], [[4oiq]], [[4oir]] – TtSF-A + RNAP subunits α,β,β’,ω + antibiotic + DNA<br />
	**[[4oio]], [[4g7h]], [[4g7o]], [[4g7z]], [[4q4z]], [[4q5s]] – TtSF SIGA + RNAP subunits α,β,β’,ω + DNA<br />		**[[4oio]], [[4g7h]], [[4g7o]], [[4g7z]], [[4q4z]], [[4q5s]] – TtSF SIGA + RNAP subunits α,β,β’,ω + DNA<br />
	**[[1smy]] – TtSF + RNAP subunits α,β,β’,ω + G4P<br />		**[[1smy]] – TtSF + RNAP subunits α,β,β’,ω + G4P<br />
Line 116:		Line 115:
	**[[5nsr]], [[6gh6]], [[6gh5]], [[6gfw]] – EcSF-54 + EcRNAP subunits α,β,β’,ω + DNA - Cryo EM<br />		**[[5nsr]], [[6gh6]], [[6gh5]], [[6gfw]] – EcSF-54 + EcRNAP subunits α,β,β’,ω + DNA - Cryo EM<br />
	**[[5voi]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA <br />		**[[5voi]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA <br />
-	**[[6cuu]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + antibiotic<br />
	**[[5d4c]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + CTP + ATP<br />		**[[5d4c]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + CTP + ATP<br />
	**[[5d4d]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + CTP + NAD<br />		**[[5d4d]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + CTP + NAD<br />
Line 123:		Line 121:
	**[[6c04]] – MtSF-A + RNAP subunits α,β,β’,ω + RbpA + DNA - Cryo EM<br />		**[[6c04]] – MtSF-A + RNAP subunits α,β,β’,ω + RbpA + DNA - Cryo EM<br />
	**[[5uhe]], [[5uhf]] – MtSF SIGA + MtRNAP subunits α,β,β’,ω + DNA + inhibitor<br />		**[[5uhe]], [[5uhf]] – MtSF SIGA + MtRNAP subunits α,β,β’,ω + DNA + inhibitor<br />
-	**[[5uhg]] – MtSF-A + MtRNAP subunits α,β,β’,ω + DNA + antibiotic + inhibitor<br />
-	**[[5uhb]] – MtSF-A + MtRNAP subunits α,β,β’,ω + DNA + antibiotic<br />
-	**[[6bzo]] – MtSF-A + RNAP subunits α,β,β’,ω + RbpA + DNA + antibiotic - Cryo EM<br />
	**[[5tw1]], [[6cce]] – MsSF-A + MtRNAP subunits α,β,β’,ω + DNA + peptide - ''Mycobacterium smegmatis''<br />		**[[5tw1]], [[6cce]] – MsSF-A + MtRNAP subunits α,β,β’,ω + DNA + peptide - ''Mycobacterium smegmatis''<br />
	**[[5vi8]] – MsSF-A + MsRNAP subunits α,β,β’,ω + DNA <br />		**[[5vi8]] – MsSF-A + MsRNAP subunits α,β,β’,ω + DNA <br />
	**[[6ccv]] – MsSF-A + MtRNAP subunits α,β,β’,ω + DNA + peptide + RbpA <br />		**[[6ccv]] – MsSF-A + MtRNAP subunits α,β,β’,ω + DNA + peptide + RbpA <br />
		+
		+	*Sigma factor complex with protein and DNA and antibiotics
		+
		+	**[[4oin]], [[4oip]], [[4oiq]], [[4oir]] – TtSF-A + RNAP subunits α,β,β’,ω + antibiotic + DNA<br />
		+	**[[6cuu]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + antibiotic<br />
		+	**[[5uhg]] – MtSF-A + MtRNAP subunits α,β,β’,ω + DNA + antibiotic + inhibitor<br />
		+	**[[5uhb]] – MtSF-A + MtRNAP subunits α,β,β’,ω + DNA + antibiotic<br />
		+	**[[6bzo]] – MtSF-A + RNAP subunits α,β,β’,ω + RbpA + DNA + antibiotic - Cryo EM<br />
	**[[6ccf]] – MsSF-A + MtRNAP subunits α,β,β’,ω + DNA + peptide + antibiotic<br />		**[[6ccf]] – MsSF-A + MtRNAP subunits α,β,β’,ω + DNA + peptide + antibiotic<br />

Michal Harel at 22:51, 19 September 2018

Michal Harel — Wed, 19 Sep 2018 22:51:30 GMT

←Older revision		Revision as of 22:51, 19 September 2018
Line 48:		Line 48:
	**[[3mzy]] – SF-H – ''Fusobacterium nucleatum''<br />		**[[3mzy]] – SF-H – ''Fusobacterium nucleatum''<br />
	**[[1h3l]] – ScSF N terminal – ''Streptomyces coelicolor''<br />		**[[1h3l]] – ScSF N terminal – ''Streptomyces coelicolor''<br />
-	**[[5fgm]] – ScSF ~~SIGR~~ region 4<br />	+	**[[5fgm]] – ScSF-R region 4<br />
-	**[[5mww]] – BsSF ~~SIGA~~ region 1.1 - ''Bacillus subtilis'' - NMR <br />	+	**[[5mww]] – BsSF-A region 1.1 - ''Bacillus subtilis'' - NMR <br />
		+	**[[5xe7]] – MtSF-J – ''Mycobacterium tuberculosis''<br />

	*Sigma factor complex with DNA		*Sigma factor complex with DNA
Line 70:		Line 71:
	**[[1rp3]], [[1sc5]] – AaSF-28 + anti-σ factor FLGM <br />		**[[1rp3]], [[1sc5]] – AaSF-28 + anti-σ factor FLGM <br />
	**[[5wuq]] – BsSF SIGW + anti-σ factor RSIW <br />		**[[5wuq]] – BsSF SIGW + anti-σ factor RSIW <br />
-	**[[3hug]] – MtSF + membrane protein ~~– ''Mycobacterium tuberculosis''~~<br />	+	**[[3hug]] – MtSF-L + membrane protein <br />
	**[[4nqw]] – MtSF-K region 4 + anti-σ factor<br />		**[[4nqw]] – MtSF-K region 4 + anti-σ factor<br />
	**[[4x8k]] – MtSF-A region 2 + RBPA<br />		**[[4x8k]] – MtSF-A region 2 + RBPA<br />
		+	**[[6fbv]] – MtSF-A + RNAP subunits α,β,β’,ω - SP Cryo EM<br />
		+	**[[6c05]], [[6c06]] – MtSF-A + RNAP subunits α,β,β’,ω + RbpA - Cryo EM<br />
	**[[3wod]], [[2a6e]], [[2cw0]], [[5tmf]] – TtSF + RNAP subunits α,β,β’,ω - ''Thermus thermophilus'' <br />		**[[3wod]], [[2a6e]], [[2cw0]], [[5tmf]] – TtSF + RNAP subunits α,β,β’,ω - ''Thermus thermophilus'' <br />
	**[[4mq9]], [[2a68]], [[2a69]], [[2a6h]], [[3dxj]], [[3eql]] – TtSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />		**[[4mq9]], [[2a68]], [[2a69]], [[2a6h]], [[3dxj]], [[3eql]] – TtSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />
	**[[2be5]] – TtSF + RNAP subunits α,β,β’,ω + inhibitor<br />		**[[2be5]] – TtSF + RNAP subunits α,β,β’,ω + inhibitor<br />
-	**[[5tmc]] – TtSF ~~SIGA~~ + RNAP subunits α,β,β’,ω + protein + G4P<br />	+	**[[5tmc]] – TtSF-A + RNAP subunits α,β,β’,ω + protein + G4P<br />
		+	**[[5xj0]] – TtSF-A + RNAP subunits α,β,β’,ω + GP39 + GP76<br />
		+	**[[5x22]] – TtSF-A + RNAP subunits α,β,β’,ω + GpA + CMPcPP<br />
	**[[1l9u]] – TaSF region 1.1-4 + RNAP subunits α,β,β’,ω<br />		**[[1l9u]] – TaSF region 1.1-4 + RNAP subunits α,β,β’,ω<br />
	**[[4yg2]], [[5uag]], [[5uaj]], [[5uaq]] – EcSF ProD + RNAP subunits α,β,β’,ω <br />		**[[4yg2]], [[5uag]], [[5uaj]], [[5uaq]] – EcSF ProD + RNAP subunits α,β,β’,ω <br />
Line 82:		Line 87:
	**[[4mex]], [[4kmu]], [[4kn4]], [[4kn7]], [[5uac]], [[5uah]], [[5ual]], [[4zh3]], [[4zh4]] – EcSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />		**[[4mex]], [[4kmu]], [[4kn4]], [[4kn7]], [[5uac]], [[5uah]], [[5ual]], [[4zh3]], [[4zh4]] – EcSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />
	**[[4xsx]], [[4xsy]], [[4xsz]] – EcSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />		**[[4xsx]], [[4xsy]], [[4xsz]] – EcSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />
-	**[[4mey]], [[4ljz]], [[4lk1]] – EcSF ProD + RNAP subunits α,β,β’,ω <br />	+	**[[6byu]] – EcSF ProD + RNAP subunits α,β,β’,ω + ppApp <br />
		+	**[[4mey]], [[4ljz]], [[4lk1]], [[6cux]] – EcSF ProD + RNAP subunits α,β,β’,ω <br />
		+	**[[6c9y]], [[6ca0]] – EcSF ProD + RNAP subunits α,β,β’,ω - Cryo EM<br />
	**[[5byh]] – EcSF-45 + RNAP subunits α,β,β’,ω <br />		**[[5byh]] – EcSF-45 + RNAP subunits α,β,β’,ω <br />
	**[[5nwt]] – EcSF-54 + RNAP subunits α,β,β’,ω <br />		**[[5nwt]] – EcSF-54 + RNAP subunits α,β,β’,ω <br />
Line 91:		Line 98:
	**[[4cxf]] – SF CNRH + CNRY – ''Cupriavidus metallidurans''<br />		**[[4cxf]] – SF CNRH + CNRY – ''Cupriavidus metallidurans''<br />
	**[[4g6d]], [[4g8x]], [[4g94]] – SF ProD region 4 + Orf067 – ''Staphylococcus aureus''<br />		**[[4g6d]], [[4g8x]], [[4g94]] – SF ProD region 4 + Orf067 – ''Staphylococcus aureus''<br />
		+	**[[5yix]] – SF RpoD + GCRA - ''Caulobacter crescentus''<br />

	*Sigma factor complex with protein and DNA		*Sigma factor complex with protein and DNA
Line 101:		Line 109:
	**[[4xlp]], [[4xlq]], [[5tjg]] – TaSF SIGA + RNAP subunits α,β,β’,ω + DNA <br />		**[[4xlp]], [[4xlq]], [[5tjg]] – TaSF SIGA + RNAP subunits α,β,β’,ω + DNA <br />
	**[[1l9u]], [[1l9z]] – TaSF + RNAP subunits α,β,β’,ω + DNA – Cryo EM <br />		**[[1l9u]], [[1l9z]] – TaSF + RNAP subunits α,β,β’,ω + DNA – Cryo EM <br />
-	**[[4xlr]], [[4xls]] – TaSF ~~SIGA~~ + RNAP subunits α,β,β’,ω + CarD-like transcriptional regulator + DNA <br />	+	**[[4xlr]], [[4xls]] – TaSF-A + RNAP subunits α,β,β’,ω + CarD-like transcriptional regulator + DNA <br />
	**[[3iyd]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + catabolite gene activator + DNA<br />		**[[3iyd]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + catabolite gene activator + DNA<br />
	**[[4jk1]], [[4jk2]], [[4jkr]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + G4P<br />		**[[4jk1]], [[4jk2]], [[4jkr]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + G4P<br />
	**[[4yln]], [[4ylo]], [[4ylp]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + DNA<br />		**[[4yln]], [[4ylo]], [[4ylp]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + DNA<br />
	**[[5nss]] – EcSF-54 + EcRNAP subunits α,β,β’,ω + phage shock protein F + DNA - Cryo EM<br />		**[[5nss]] – EcSF-54 + EcRNAP subunits α,β,β’,ω + phage shock protein F + DNA - Cryo EM<br />
-	**[[5nsr]] – EcSF-54 + EcRNAP subunits α,β,β’,ω + DNA - Cryo EM<br />	+	**[[5nsr]], [[6gh6]], [[6gh5]], [[6gfw]] – EcSF-54 + EcRNAP subunits α,β,β’,ω + DNA - Cryo EM<br />
-	**[[5voi]] – TtSF ~~SIGA~~ + TtRNAP subunits α,β,β’,ω + DNA <br />	+	**[[5voi]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA <br />
-	**[[5d4c]] – TtSF ~~SIGA~~ + TtRNAP subunits α,β,β’,ω + DNA + CTP + ATP<br />	+	**[[6cuu]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + antibiotic<br />
-	**[[5d4d]] – TtSF ~~SIGA~~ + TtRNAP subunits α,β,β’,ω + DNA + CTP + NAD<br />	+	**[[5d4c]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + CTP + ATP<br />
-	**[[5d4e]] – TtSF ~~SIGA~~ + TtRNAP subunits α,β,β’,ω + DNA + CTP + dephosphate-CoA<br />	+	**[[5d4d]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + CTP + NAD<br />
-	**[[5uha]] – MtSF ~~SIGA~~ + MtRNAP subunits α,β,β’,ω + DNA <br />	+	**[[5d4e]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + CTP + dephosphate-CoA<br />
		+	**[[5uha]] – MtSF-A + MtRNAP subunits α,β,β’,ω + DNA <br />
		+	**[[6c04]] – MtSF-A + RNAP subunits α,β,β’,ω + RbpA + DNA - Cryo EM<br />
	**[[5uhe]], [[5uhf]] – MtSF SIGA + MtRNAP subunits α,β,β’,ω + DNA + inhibitor<br />		**[[5uhe]], [[5uhf]] – MtSF SIGA + MtRNAP subunits α,β,β’,ω + DNA + inhibitor<br />
-	**[[5uhg]] – MtSF ~~SIGA~~ + MtRNAP subunits α,β,β’,ω + DNA + antibiotic + inhibitor<br />	+	**[[5uhg]] – MtSF-A + MtRNAP subunits α,β,β’,ω + DNA + antibiotic + inhibitor<br />
-	**[[5uhb]] – MtSF ~~SIGA~~ + MtRNAP subunits α,β,β’,ω + DNA + antibiotic<br />	+	**[[5uhb]] – MtSF-A + MtRNAP subunits α,β,β’,ω + DNA + antibiotic<br />
-	**[[5tw1]] – MsSF ~~SIGA~~ + MtRNAP subunits α,β,β’,ω + DNA + peptide - ''Mycobacterium smegmatis''<br />	+	**[[6bzo]] – MtSF-A + RNAP subunits α,β,β’,ω + RbpA + DNA + antibiotic - Cryo EM<br />
-	**[[5vi8]] – MsSF ~~SIGA~~ + MsRNAP subunits α,β,β’,ω + DNA <br />	+	**[[5tw1]], [[6cce]] – MsSF-A + MtRNAP subunits α,β,β’,ω + DNA + peptide - ''Mycobacterium smegmatis''<br />
		+	**[[5vi8]] – MsSF-A + MsRNAP subunits α,β,β’,ω + DNA <br />
		+	**[[6ccv]] – MsSF-A + MtRNAP subunits α,β,β’,ω + DNA + peptide + RbpA <br />
		+	**[[6ccf]] – MsSF-A + MtRNAP subunits α,β,β’,ω + DNA + peptide + antibiotic<br />

	*Sigma factor complex with protein, DNA and RNA		*Sigma factor complex with protein, DNA and RNA

-	**[[5vi5]] – MsSF ~~SIGA~~ + MsRNAP subunits α,β,β’,ω + RbpA + DNA + RNA<br />	+	**[[5vi5]] – MsSF-A + MsRNAP subunits α,β,β’,ω + RbpA + DNA + RNA<br />
-	**[[4xln]] – TaSF ~~SIGA~~ + RNAP subunits α,β,β’,ω + DNA + RNA <br />	+	**[[4xln]] – TaSF-A + RNAP subunits α,β,β’,ω + DNA + RNA <br />
	**[[5ipl]], [[5ipm]], [[5ipn]] – EcSF RpoS + EcRNAP subunits α,β,β’,ω + DNA + RNA<br />		**[[5ipl]], [[5ipm]], [[5ipn]] – EcSF RpoS + EcRNAP subunits α,β,β’,ω + DNA + RNA<br />
-	**[[5e17]], [[5e18]] – TtSF ~~SIGA~~ + TtRNAP subunits α,β,β’,ω + DNA + RNA<br />	+	**[[6b6h]] – EcSF RpoD + EcRNAP subunits α,β,β’,ω + DNA + RNA - Cryo EM<br />
-	**[[5x22]] – TtSF ~~SIGA~~ + TtRNAP subunits α,β,β’,ω + DNA + RNA + CMPPP<br />	+	**[[5e17]], [[5e18]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + RNA<br />
-	**[[5vo8]] – TtSF ~~SIGA~~ + TtRNAP subunits α,β,β’,ω + DNA + RNA + GTP<br />	+	**[[5x22]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + RNA + CMPPP<br />
-	**[[5i2d]] – TtSF ~~SIGA~~ + TtRNAP subunits α,β,β’,ω + transcriptional regulator + DNA + RNA<br />	+	**[[5vo8]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + RNA + GTP<br />
-	**[[5uh5]], [[5uh8]], [[5uh9]] – MtSF ~~SIGA~~ + MtRNAP subunits α,β,β’,ω + DNA + RNA<br />	+	**[[5x21]] – TtSF-A + TtRNAP subunits α,β,β’,ω + DNA + RNA + antibiotic<br />
-	**[[5uh6]], [[5uhc]], [[5uhd]], [[5x21]] – MtSF ~~SIGA~~ + TtRNAP subunits α,β,β’,ω + DNA + RNA + antibiotic<br />	+	**[[5i2d]] – TtSF-A + TtRNAP subunits α,β,β’,ω + transcriptional regulator + DNA + RNA<br />
		+	**[[5uh5]], [[5uh8]], [[5uh9]] – MtSF-A + MtRNAP subunits α,β,β’,ω + DNA + RNA<br />
		+	**[[5uh6]], [[5uhc]], [[5uhd]], [[5x21]] – MtSF-A + TtRNAP subunits α,β,β’,ω + DNA + RNA + antibiotic<br />
	}}		}}
	== References ==		== References ==

Michal Harel at 21:54, 19 September 2018

Michal Harel — Wed, 19 Sep 2018 21:54:36 GMT

←Older revision		Revision as of 21:54, 19 September 2018
Line 80:		Line 80:
	**[[4yg2]], [[5uag]], [[5uaj]], [[5uaq]] – EcSF ProD + RNAP subunits α,β,β’,ω <br />		**[[4yg2]], [[5uag]], [[5uaj]], [[5uaq]] – EcSF ProD + RNAP subunits α,β,β’,ω <br />
	**[[5ui8]] – EcSF-45 + RNAP subunits α,β,β’,ω <br />		**[[5ui8]] – EcSF-45 + RNAP subunits α,β,β’,ω <br />
-	**[[4mex]], [[4kmu]], [[4kn4]], [[4kn7]], [[5uac]], [[5uah]], [[~~5tual~~]], [[4zh3]], [[4zh4]] – EcSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />	+	**[[4mex]], [[4kmu]], [[4kn4]], [[4kn7]], [[5uac]], [[5uah]], [[5ual]], [[4zh3]], [[4zh4]] – EcSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />
	**[[4xsx]], [[4xsy]], [[4xsz]] – EcSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />		**[[4xsx]], [[4xsy]], [[4xsz]] – EcSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />
	**[[4mey]], [[4ljz]], [[4lk1]] – EcSF ProD + RNAP subunits α,β,β’,ω <br />		**[[4mey]], [[4ljz]], [[4lk1]] – EcSF ProD + RNAP subunits α,β,β’,ω <br />

Michal Harel at 08:03, 26 October 2017

Michal Harel — Thu, 26 Oct 2017 08:03:26 GMT

←Older revision		Revision as of 08:03, 26 October 2017
Line 1:		Line 1:
-	<StructureSection load='4lup' size='340' side='right' caption='Structure of sigma factor of E.Coli RNAP (grey) in complex with ~~Promoter~~ DNA ~~(green)~~ and ethylene glycol (PDB code [[4lup]]).' scene=''>	+	<StructureSection load='4lup' size='340' side='right' caption='Structure of sigma factor of E.Coli RNAP (grey) in complex with promoter DNA and ethylene glycol (PDB code [[4lup]]).' scene=''>

	== Overview ==		== Overview ==

Michal Harel at 18:49, 4 October 2017

Michal Harel — Wed, 04 Oct 2017 18:49:55 GMT

←Older revision		Revision as of 18:49, 4 October 2017
Line 47:		Line 47:
	**[[2k9l]], [[2k9m]] – AaSF-54 core domain - NMR<br />		**[[2k9l]], [[2k9m]] – AaSF-54 core domain - NMR<br />
	**[[3mzy]] – SF-H – ''Fusobacterium nucleatum''<br />		**[[3mzy]] – SF-H – ''Fusobacterium nucleatum''<br />
-	**[[1h3l]] – SF N terminal – ''Streptomyces coelicolor''<br />	+	**[[1h3l]] – ScSF N terminal – ''Streptomyces coelicolor''<br />
		+	**[[5fgm]] – ScSF SIGR region 4<br />
		+	**[[5mww]] – BsSF SIGA region 1.1 - ''Bacillus subtilis'' - NMR <br />

	*Sigma factor complex with DNA		*Sigma factor complex with DNA
Line 57:		Line 59:
	**[[1ku7]] – EcSF region 4 (mutant) + DNA <br />		**[[1ku7]] – EcSF region 4 (mutant) + DNA <br />
	**[[2o8k]], [[2o9l]] – AaSF-54 C terminal + DNA - NMR<br />		**[[2o8k]], [[2o9l]] – AaSF-54 C terminal + DNA - NMR<br />
		+	**[[5ui5]] – AaSF RpoN residues 61-398 + DNA <br />

	*Sigma factor complex with protein		*Sigma factor complex with protein
Line 63:		Line 66:
	**[[1or7]] – EcSF-E + σ-E factor negative regulatory protein <br />		**[[1or7]] – EcSF-E + σ-E factor negative regulatory protein <br />
	**[[4lup]] – EcSF residues 3-92 + EcSF region 2 <br />		**[[4lup]] – EcSF residues 3-92 + EcSF region 2 <br />
-	**[[1tlh]] – EcSF~~-70~~ region 4 + anti-σ factor<br />	+	**[[1tlh]] – EcSF ProD region 4 + anti-σ factor<br />
-	**[[2p7v]] – EcSF~~-70~~ region 4 + regulator of σ D<br />	+	**[[2p7v]] – EcSF ProD region 4 + regulator of σ D<br />
	**[[1rp3]], [[1sc5]] – AaSF-28 + anti-σ factor FLGM <br />		**[[1rp3]], [[1sc5]] – AaSF-28 + anti-σ factor FLGM <br />
		+	**[[5wuq]] – BsSF SIGW + anti-σ factor RSIW <br />
	**[[3hug]] – MtSF + membrane protein – ''Mycobacterium tuberculosis''<br />		**[[3hug]] – MtSF + membrane protein – ''Mycobacterium tuberculosis''<br />
	**[[4nqw]] – MtSF-K region 4 + anti-σ factor<br />		**[[4nqw]] – MtSF-K region 4 + anti-σ factor<br />
	**[[4x8k]] – MtSF-A region 2 + RBPA<br />		**[[4x8k]] – MtSF-A region 2 + RBPA<br />
-	**[[3wod]], [[2a6e]], [[2cw0]] – TtSF + RNAP subunits α,β,β’,ω - ''Thermus thermophilus'' <br />	+	**[[3wod]], [[2a6e]], [[2cw0]], [[5tmf]] – TtSF + RNAP subunits α,β,β’,ω - ''Thermus thermophilus'' <br />
-	**[[4mq9]], [[2a68]], [[2a69]], [[2a6h]], [[3dxj]], [[3eql]] – TtSF~~-70~~ + RNAP subunits α,β,β’,ω + antibiotic <br />	+	**[[4mq9]], [[2a68]], [[2a69]], [[2a6h]], [[3dxj]], [[3eql]] – TtSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />
	**[[2be5]] – TtSF + RNAP subunits α,β,β’,ω + inhibitor<br />		**[[2be5]] – TtSF + RNAP subunits α,β,β’,ω + inhibitor<br />
		+	**[[5tmc]] – TtSF SIGA + RNAP subunits α,β,β’,ω + protein + G4P<br />
	**[[1l9u]] – TaSF region 1.1-4 + RNAP subunits α,β,β’,ω<br />		**[[1l9u]] – TaSF region 1.1-4 + RNAP subunits α,β,β’,ω<br />
-	**[[4yg2]] – EcSF-70 + RNAP subunits α,β,β’,ω <br />	+	**[[4yg2]], [[5uag]], [[5uaj]], [[5uaq]] – EcSF ProD + RNAP subunits α,β,β’,ω <br />
-	**[[4mex]], [[4kmu]], [[4kn4]], [[4kn7]] – EcSF~~-70~~ + RNAP subunits α,β,β’,ω + antibiotic <br />	+	**[[5ui8]] – EcSF-45 + RNAP subunits α,β,β’,ω <br />
-	**[[4mey]], [[4ljz]], [[4lk1]] – EcSF-70 + RNAP subunits α,β,β’,ω <br />	+	**[[4mex]], [[4kmu]], [[4kn4]], [[4kn7]], [[5uac]], [[5uah]], [[5tual]], [[4zh3]], [[4zh4]] – EcSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />
-	**[[4lk0]], [[4llg]], [[4yfk]], [[4yfn]], [[4yfx]] – EcSF~~-70~~ + EcRNAP subunits α,β,β’,ω + RNAP inhibitor<br />	+	**[[4xsx]], [[4xsy]], [[4xsz]] – EcSF ProD + RNAP subunits α,β,β’,ω + antibiotic <br />
		+	**[[4mey]], [[4ljz]], [[4lk1]] – EcSF ProD + RNAP subunits α,β,β’,ω <br />
		+	**[[5byh]] – EcSF-45 + RNAP subunits α,β,β’,ω <br />
		+	**[[5nwt]] – EcSF-54 + RNAP subunits α,β,β’,ω <br />
		+	**[[5w1s]] – EcSF RpoD + RNAP subunits α,β,β’ + TraR<br />
		+	**[[5vsw]] – EcSF RpoD + RNAP subunits α,β,β’ + DnaK suppressor protein<br />
		+	**[[5w1t]] – EcSF RpoD + RNAP subunits α,β,β’ + RpoZ + DnaK suppressor protein<br />
		+	**[[4lk0]], [[4llg]], [[4yfk]], [[4yfn]], [[4yfx]], [[4zh2]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + RNAP inhibitor<br />
	**[[4cxf]] – SF CNRH + CNRY – ''Cupriavidus metallidurans''<br />		**[[4cxf]] – SF CNRH + CNRY – ''Cupriavidus metallidurans''<br />
-	**[[4g6d]], [[4g8x]], [[4g94]] – SF~~-70~~ region 4 + Orf067 – ''Staphylococcus aureus''<br />	+	**[[4g6d]], [[4g8x]], [[4g94]] – SF ProD region 4 + Orf067 – ''Staphylococcus aureus''<br />

	*Sigma factor complex with protein and DNA		*Sigma factor complex with protein and DNA
Line 84:		Line 96:
	**[[1rio]] - TaSF region 4 + repression protein CI + DNA<br />		**[[1rio]] - TaSF region 4 + repression protein CI + DNA<br />
	**[[4oin]], [[4oip]], [[4oiq]], [[4oir]] – TtSF-A + RNAP subunits α,β,β’,ω + antibiotic + DNA<br />		**[[4oin]], [[4oip]], [[4oiq]], [[4oir]] – TtSF-A + RNAP subunits α,β,β’,ω + antibiotic + DNA<br />
-	**[[4oio]], [[4g7h]], [[4g7o]], [[4g7z]], [[4q4z]], [[4q5s]] – TtSF-A + RNAP subunits α,β,β’,ω + DNA<br />	+	**[[4oio]], [[4g7h]], [[4g7o]], [[4g7z]], [[4q4z]], [[4q5s]] – TtSF SIGA + RNAP subunits α,β,β’,ω + DNA<br />
	**[[1smy]] – TtSF + RNAP subunits α,β,β’,ω + G4P<br />		**[[1smy]] – TtSF + RNAP subunits α,β,β’,ω + G4P<br />
	**[[3n97]] – TaSF region 4 + RNAP subunits α + DNA<br />		**[[3n97]] – TaSF region 4 + RNAP subunits α + DNA<br />
		+	**[[4xlp]], [[4xlq]], [[5tjg]] – TaSF SIGA + RNAP subunits α,β,β’,ω + DNA <br />
	**[[1l9u]], [[1l9z]] – TaSF + RNAP subunits α,β,β’,ω + DNA – Cryo EM <br />		**[[1l9u]], [[1l9z]] – TaSF + RNAP subunits α,β,β’,ω + DNA – Cryo EM <br />
-	**[[3iyd]] – EcSF~~-70~~ + EcRNAP subunits α,β,β’,ω + catabolite gene activator + DNA<br />	+	**[[4xlr]], [[4xls]] – TaSF SIGA + RNAP subunits α,β,β’,ω + CarD-like transcriptional regulator + DNA <br />
-	**[[4jk1]], [[4jk2]], [[4jkr]] – EcSF~~-70~~ + EcRNAP subunits α,β,β’,ω + G4P<br />	+	**[[3iyd]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + catabolite gene activator + DNA<br />
-	**[[4yln]], [[4ylo]], [[4ylp]] – EcSF-70 + EcRNAP subunits α,β,β’,ω + DNA<br />	+	**[[4jk1]], [[4jk2]], [[4jkr]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + G4P<br />
		+	**[[4yln]], [[4ylo]], [[4ylp]] – EcSF ProD + EcRNAP subunits α,β,β’,ω + DNA<br />
		+	**[[5nss]] – EcSF-54 + EcRNAP subunits α,β,β’,ω + phage shock protein F + DNA - Cryo EM<br />
		+	**[[5nsr]] – EcSF-54 + EcRNAP subunits α,β,β’,ω + DNA - Cryo EM<br />
		+	**[[5voi]] – TtSF SIGA + TtRNAP subunits α,β,β’,ω + DNA <br />
		+	**[[5d4c]] – TtSF SIGA + TtRNAP subunits α,β,β’,ω + DNA + CTP + ATP<br />
		+	**[[5d4d]] – TtSF SIGA + TtRNAP subunits α,β,β’,ω + DNA + CTP + NAD<br />
		+	**[[5d4e]] – TtSF SIGA + TtRNAP subunits α,β,β’,ω + DNA + CTP + dephosphate-CoA<br />
		+	**[[5uha]] – MtSF SIGA + MtRNAP subunits α,β,β’,ω + DNA <br />
		+	**[[5uhe]], [[5uhf]] – MtSF SIGA + MtRNAP subunits α,β,β’,ω + DNA + inhibitor<br />
		+	**[[5uhg]] – MtSF SIGA + MtRNAP subunits α,β,β’,ω + DNA + antibiotic + inhibitor<br />
		+	**[[5uhb]] – MtSF SIGA + MtRNAP subunits α,β,β’,ω + DNA + antibiotic<br />
		+	**[[5tw1]] – MsSF SIGA + MtRNAP subunits α,β,β’,ω + DNA + peptide - ''Mycobacterium smegmatis''<br />
		+	**[[5vi8]] – MsSF SIGA + MsRNAP subunits α,β,β’,ω + DNA <br />

		+	*Sigma factor complex with protein, DNA and RNA
		+
		+	**[[5vi5]] – MsSF SIGA + MsRNAP subunits α,β,β’,ω + RbpA + DNA + RNA<br />
		+	**[[4xln]] – TaSF SIGA + RNAP subunits α,β,β’,ω + DNA + RNA <br />
		+	**[[5ipl]], [[5ipm]], [[5ipn]] – EcSF RpoS + EcRNAP subunits α,β,β’,ω + DNA + RNA<br />
		+	**[[5e17]], [[5e18]] – TtSF SIGA + TtRNAP subunits α,β,β’,ω + DNA + RNA<br />
		+	**[[5x22]] – TtSF SIGA + TtRNAP subunits α,β,β’,ω + DNA + RNA + CMPPP<br />
		+	**[[5vo8]] – TtSF SIGA + TtRNAP subunits α,β,β’,ω + DNA + RNA + GTP<br />
		+	**[[5i2d]] – TtSF SIGA + TtRNAP subunits α,β,β’,ω + transcriptional regulator + DNA + RNA<br />
		+	**[[5uh5]], [[5uh8]], [[5uh9]] – MtSF SIGA + MtRNAP subunits α,β,β’,ω + DNA + RNA<br />
		+	**[[5uh6]], [[5uhc]], [[5uhd]], [[5x21]] – MtSF SIGA + TtRNAP subunits α,β,β’,ω + DNA + RNA + antibiotic<br />
	}}		}}
	== References ==		== References ==

Michal Harel at 13:26, 29 August 2016

Michal Harel — Mon, 29 Aug 2016 13:26:14 GMT

←Older revision		Revision as of 13:26, 29 August 2016
Line 1:		Line 1:
-	<StructureSection load='4lup' size='340' side='right' caption='Structure of sigma factor of E.Coli RNAP in complex with Promoter DNA (PDB code [[4lup]]).' scene=''>	+	<StructureSection load='4lup' size='340' side='right' caption='Structure of sigma factor of E.Coli RNAP (grey) in complex with Promoter DNA (green) and ethylene glycol (PDB code [[4lup]]).' scene=''>

-	[[Image:2h27.jpg\|left\|200px]]
	== Overview ==		== Overview ==