Strictosidine Synthase - Revision history

Michal Harel at 08:46, 6 December 2023

Michal Harel — Wed, 06 Dec 2023 08:46:44 GMT

←Older revision		Revision as of 08:46, 6 December 2023
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	[[2vaq]] – sSTR1 + inhibitor<br />		[[2vaq]] – sSTR1 + inhibitor<br />
-	[[6n5v]] – sSTR + indole derivative <br />	+	[[6n5v]], [[7t5i]], [[7t5j]] – sSTR + indole derivative <br />
	[[4imb]], [[4iyg]] – dSTR + indole derivative <br />		[[4imb]], [[4iyg]] – dSTR + indole derivative <br />
	[[6s5q]], [[6s5u]], [[6s5j]], [[6s5m]] – STR + carboline derivative – ''Ophiorrhiza pumila''<br />		[[6s5q]], [[6s5u]], [[6s5j]], [[6s5m]] – STR + carboline derivative – ''Ophiorrhiza pumila''<br />

Michal Harel at 08:37, 12 November 2020

Michal Harel — Thu, 12 Nov 2020 08:37:40 GMT

←Older revision		Revision as of 08:37, 12 November 2020
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	[[6n5v]] – sSTR + indole derivative <br />		[[6n5v]] – sSTR + indole derivative <br />
	[[4imb]], [[4iyg]] – dSTR + indole derivative <br />		[[4imb]], [[4iyg]] – dSTR + indole derivative <br />
		+	[[6s5q]], [[6s5u]], [[6s5j]], [[6s5m]] – STR + carboline derivative – ''Ophiorrhiza pumila''<br />
		+	[[6zea]] – STR + carboline derivative – periwinkle<br />
	== References ==		== References ==
	<references/>		<references/>
	[[Category: Topic Page]]		[[Category: Topic Page]]

Michal Harel at 08:34, 10 February 2020

Michal Harel — Mon, 10 Feb 2020 08:34:12 GMT

←Older revision		Revision as of 08:34, 10 February 2020
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	[[2vaq]] – sSTR1 + inhibitor<br />		[[2vaq]] – sSTR1 + inhibitor<br />
		+	[[6n5v]] – sSTR + indole derivative <br />
	[[4imb]], [[4iyg]] – dSTR + indole derivative <br />		[[4imb]], [[4iyg]] – dSTR + indole derivative <br />
	== References ==		== References ==
	<references/>		<references/>
	[[Category: Topic Page]]		[[Category: Topic Page]]

Alexander Berchansky at 13:08, 15 September 2019

Alexander Berchansky — Sun, 15 Sep 2019 13:08:33 GMT

←Older revision		Revision as of 13:08, 15 September 2019
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	== Structural highlights ==		== Structural highlights ==
-	The <scene name='10/100149/Cv/5'>substrate binding pocket of STR1 is located near the pseudo six-fold symmetry axis</scene><ref>PMID:16531499</ref>. <scene name='10/100149/Cv/7'>Active site</scene>. Water molecule shown as red sphere.	+	The <scene name='10/100149/Cv/8'>substrate binding pocket of STR1 is located near the pseudo six-fold symmetry axis</scene><ref>PMID:16531499</ref>. {{Template:ColorKey_Helix}},
		+	{{Template:ColorKey_Strand}},
		+	{{Template:ColorKey_Loop}},
		+	{{Template:ColorKey_Turn}}. <scene name='10/100149/Cv/9'>Active site</scene>. Water molecule are shown as red sphere.
	</StructureSection>		</StructureSection>
	==3D structures of strictosidine synthase==		==3D structures of strictosidine synthase==

Michal Harel at 22:00, 20 September 2018

Michal Harel — Thu, 20 Sep 2018 22:00:29 GMT

←Older revision		Revision as of 22:00, 20 September 2018
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	== Function ==		== Function ==
-	The enzyme '''strictosidine synthase''' (<scene name='Strictisidine_Synthase/Str1_sp/1'>STR1</scene>) (EC 4.3.3.2) from an Indian medicinal plant Rauvolfia serpentina is of primary importance for the biosynthetic pathway of the indole alkaloid ajmaline. STR1 initiates all biosynthetic pathways leading to the entire monoterpenoid indole alkaloid family representing an enormous structural variety of ~2000 compounds in higher plants. The enzyme is involved in the biosynthesis of all these alkaloids by catalyzing the condensation of the two initial building blocks, tryptamine and the monoterpenoid secologanin, leading to the glucoalkaloid strictosidine. The reaction type catalyzed by STR1 is so far an exceptional example in the biosynthesis of natural products. It was hitherto known only from synthetic chemistry (Pictet-Spengler–type reaction), where it is applied in alkaloid synthesis, especially of tetrahydroisoquinolines by condensation of an amine and an aldehyde under acidic conditions<ref>PMID:18280746</ref>.	+	The enzyme '''strictosidine synthase''' (<scene name='Strictisidine_Synthase/Str1_sp/1'>STR1</scene>) (EC 4.3.3.2) from an Indian medicinal plant ''Rauvolfia serpentina'' is of primary importance for the biosynthetic pathway of the indole alkaloid ajmaline. STR1 initiates all biosynthetic pathways leading to the entire monoterpenoid indole alkaloid family representing an enormous structural variety of ~2000 compounds in higher plants. The enzyme is involved in the biosynthesis of all these alkaloids by catalyzing the condensation of the two initial building blocks, tryptamine and the monoterpenoid secologanin, leading to the glucoalkaloid strictosidine. The reaction type catalyzed by STR1 is so far an exceptional example in the biosynthesis of natural products. It was hitherto known only from synthetic chemistry (Pictet-Spengler–type reaction), where it is applied in alkaloid synthesis, especially of tetrahydroisoquinolines by condensation of an amine and an aldehyde under acidic conditions<ref>PMID:18280746</ref>.

	The overall structure of the enzyme contains a six-bladed four-stranded ß-propeller fold. All six blades are radially arranged around a pseudo six-fold symmetry axis. Each blade contains a twisted four-stranded antiparallel ß-sheet.		The overall structure of the enzyme contains a six-bladed four-stranded ß-propeller fold. All six blades are radially arranged around a pseudo six-fold symmetry axis. Each blade contains a twisted four-stranded antiparallel ß-sheet.

Michal Harel at 21:59, 20 September 2018

Michal Harel — Thu, 20 Sep 2018 21:59:55 GMT

←Older revision		Revision as of 21:59, 20 September 2018
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-	<StructureSection load='2fpb' size='~~450~~' side='right' caption='Strictosidine synthase dimer complex with tryptamine (PDB entry [[2fpb]])' scene='10/100149/Cv/3'>	+	<StructureSection load='2fpb' size='350' side='right' caption='Strictosidine synthase dimer complex with tryptamine (PDB entry [[2fpb]])' scene='10/100149/Cv/3'>

Alexander Berchansky at 11:08, 1 September 2016

Alexander Berchansky — Thu, 01 Sep 2016 11:08:12 GMT

←Older revision		Revision as of 11:08, 1 September 2016
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	== Structural highlights ==		== Structural highlights ==
-	The <scene name='10/100149/Cv/5'>substrate binding pocket of STR1 is located near the pseudo six-fold symmetry axis</scene><ref>PMID:16531499</ref>.	+	The <scene name='10/100149/Cv/5'>substrate binding pocket of STR1 is located near the pseudo six-fold symmetry axis</scene><ref>PMID:16531499</ref>. <scene name='10/100149/Cv/7'>Active site</scene>. Water molecule shown as red sphere.
	</StructureSection>		</StructureSection>
	==3D structures of strictosidine synthase==		==3D structures of strictosidine synthase==

Alexander Berchansky at 10:59, 1 September 2016

Alexander Berchansky — Thu, 01 Sep 2016 10:59:02 GMT

←Older revision		Revision as of 10:59, 1 September 2016
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	== Structural highlights ==		== Structural highlights ==
-	The substrate binding pocket of STR1 is located near the pseudo six-fold symmetry axis<ref>PMID:16531499</ref>.	+	The <scene name='10/100149/Cv/5'>substrate binding pocket of STR1 is located near the pseudo six-fold symmetry axis</scene><ref>PMID:16531499</ref>.
	</StructureSection>		</StructureSection>
	==3D structures of strictosidine synthase==		==3D structures of strictosidine synthase==

Alexander Berchansky at 10:55, 1 September 2016

Alexander Berchansky — Thu, 01 Sep 2016 10:55:12 GMT

←Older revision		Revision as of 10:55, 1 September 2016
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-		+	<StructureSection load='2fpb' size='450' side='right' caption='Strictosidine synthase dimer complex with tryptamine (PDB entry [[2fpb]])' scene='10/100149/Cv/3'>
-	<StructureSection load='2fpb' size='~~350~~' side='right' caption='Strictosidine synthase dimer complex with tryptamine (PDB entry [[2fpb]])' scene=''>	+

Michal Harel at 10:35, 1 September 2016

Michal Harel — Thu, 01 Sep 2016 10:35:19 GMT

←Older revision		Revision as of 10:35, 1 September 2016
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-	~~{{STRUCTURE_2vaq\| PDB~~=~~2vaq \| SIZE~~=~~400\| SCENE~~= \|right~~\|CAPTION~~=Strictosidine synthase dimer complex with ~~inhibitor,~~ [[~~2vaq~~]] }}	+
		+	<StructureSection load='2fpb' size='350' side='right' caption='Strictosidine synthase dimer complex with tryptamine (PDB entry [[2fpb]])' scene=''>


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	== Structural highlights ==		== Structural highlights ==
-	The substrate binding pocket of STR1 is located near the pseudo six-fold symmetry axis	+	The substrate binding pocket of STR1 is located near the pseudo six-fold symmetry axis<ref>PMID:16531499</ref>.
-		+	</StructureSection>
	==3D structures of strictosidine synthase==		==3D structures of strictosidine synthase==