TRNA methyltransferase - Revision history

Michal Harel at 09:26, 10 March 2020

Michal Harel — Tue, 10 Mar 2020 09:26:12 GMT

←Older revision		Revision as of 09:26, 10 March 2020
Line 12:		Line 12:
	</StructureSection>		</StructureSection>

-	==3D structures of tRNA methyltransferase==
-
-	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
-	{{#tree:id=OrganizedByTopic\|openlevels=0\|
-
-	*'''tRNA guanine 9 N1 methyltransferase'''
-
-	**[[4jwg]] – fyTrm – fission yeast<br />
-	**[[4jwf]], [[4jwh]] – fyTrm + SAH <br />
-	**[[4jwj]] – yTrm + SAH – yeast<br />
-
-	*'''tRNA guanine 37 N1 methyltransferase'''
-
-	**[[3knu]], [[6uev]] – ApTrm – ''Anaplasma phagocytophilum'' <br />
-	**[[1oy5]] – AaTrm – ''Aquifex aeolicus'' <br />
-	**[[3ief]] – Trm – ''Bartonella henselae'' <br />
-	**[[1uaj]] – HiTrm – ''Haemophilus influenzae''<br />
-	**[[3ky7]] – Trm – ''Staphylococcus aureus''<br />
-	**[[3quv]], [[6nvr]] – MaTrm – ''Mycobacterium abscessus''<br />
-	**[[5hjj]], [[5yac]] – PabTrm – ''Pyrococcus abyssi''<br />
-	**[[6jki]] – PaTrm – ''Pseudomonas aeruginosa''<br />
-	**[[5zhi]] – MtTrm – ''Mycobacterium tuberculosis''<br />
-
-	*Complexes of tRNA guanine 37 N1 methyltransferase
-
-	**[[1uak]], [[4yvg]] – HiTrm + SAM <br />
-	**[[1ual]], [[1uam]] – HiTrm + SAH <br />
-	**[[3axz]] – HiTrm + adenosine<br />
-	**[[4mcb]], [[4mcc]], [[4mcd]] – HiTrm + inhibitor<br />
-	**[[4yvh]] – HiTrm + SFG <br />
-	**[[4yvi]], [[4yvj]], [[4yvk]] – HiTrm + SFG + tRNA<br />
-	**[[5d9f]], [[4ypw]], [[4ypx]], [[4ypy]], [[4ypz]], [[4yq0]], [[4yq1]], [[4yq2]], [[4yq3]], [[4yq4]], [[4yq5]], [[4yq6]], [[4yq7]], [[4yq8]], [[4yq9]], [[4yqa]], [[4yqb]], [[4yqc]], [[4yqg]], [[4yqi]], [[4yqj]], [[4yqk]], [[4yql]], [[4yqn]], [[4yqo]], [[4yqp]], [[4yqq]], [[4yqr]], [[4yqs]], [[4yqt]], [[4yqd]] – HiTrm + SAM competitive<br />
-	**[[2yx1]] – MjTrm + SFG – ''Methanocalcococcus jannaschii''<br />
-	**[[3ay0]] – MjTrm + adenosine<br />
-	**[[4h3y]], [[4h3z]] – Trm + SAH – ''Burkholderia phymatum'' <br />
-	**[[4ig6]] – ApTrm + SAH <br />
-	**[[1p9p]] – EcTrm + SAH – ''Escherichia coli'' <br />
-	**[[4gek]], [[4iwn]] – EcTrm + SAM derivative<br />
-	**[[5hr7]] – EcTrm + tRNA-Glu<br />
-	**[[5hji]], [[5hjm]] – PabTrm + adenosine derivative<br />
-	**[[5hjk]] – PabTrm + SAH <br />
-	**[[6qo2]], [[6qo3]], [[6qo4]], [[6qo6]], [[6qoa]], [[6qoc]], [[6qod]], [[6qoe]], [[6qof]], [[6qog]], [[6qoh]], [[6qoi]], [[6qoj]], [[6qok]], [[6qol]], [[6qom]], [[6qon]], [[6qoo]], [[6qop]], [[6qoq]], [[6qor]], [[6qos]], [[6qot]], [[6qou]], [[6qov]], [[6qow]], [[6qox]], [[6qqq]], [[6qqr]], [[6qqt]], [[6qqu]], [[6qqv]], [[6qqw]], [[6qqz]], [[6qr0]], [[6qr1]], [[6qr2]], [[6qr3]], [[6qr4]], [[6qr7]], [[6qr9]], [[6qra]], [[6qrc]], [[6qrd]], [[6qre]], [[6qrf]], [[6qrg]] – MaTrm + inhibitor<br />
-	**[[6nw6]] – MaTrm + SAM <br />
-	**[[6nw7]] – MaTrm + SAH <br />
-	**[[6joe]], [[6jof]], [[5zhm]], [[5zhn]], [[6afk]] – PaTrm + inhibitor<br />
-	**[[6wyq]] – PaTrm + SAM <br />
-	**[[6wyr]] – PaTrm + SFG <br />
-	**[[5zhj]] – MtTrm + SAH <br />
-	**[[5zhk]], [[5zhl]] – MtTrm + inhibitor<br />
-	**[[5wt1]] – PabTrm + tRNA + SAH <br />
-	**[[5wt3]] – PabTrm + tRNA + methylthioadenosine<br />
-
-	*'''tRNA guanine 46 N7 methyltransferase'''
-
-	**[[2vdu]], [[2vdv]] – yTrm <br />
-	**[[1yzh]] – Trm – ''Streptococcus pneumoniae'' <br />
-	**[[2fca]] – BsTrm – ''Bacillus subtilis'' <br />
-	**[[3dxx]] – EcTrm <br />
-
-	*Complexes of tRNA guanine 46 N7 methyltransferase
-
-	**[[3ckk]] – hTrm + SAM - human<br />
-	**[[3dxy]] – EcTrm + SAM <br />
-	**[[3dxz]] – EcTrm + SAH <br />
-
-	*Trm to O2 of cytidine 32
-
-	**[[4cnd]] – EcTrm (mutant)<br />
-	**[[4xbo]] – EcTrm + SAH<br />
-	**[[4cne]] – EcTrm (mutant) + SAH<br />
-
-	*'''tRNA cytidine 34 O2 methyltransferase'''
-
-	**[[6jp6]] – yTrm <br />
-	**[[3e5y]] – BpTrm – ''Burkholderia pseudomalllei'' <br />
-	**[[4jak]], [[4kdz]] – EcTrm <br />
-	**[[6qkv]] – PaTrm <br />
-	**[[6ahw]] – HiTrm <br />
-
-	*Complexes of tRNA cytidine 34 O2 methyltransferase
-
-	**[[6jpl]] – yTrm + SAM <br />
-	**[[4kgn]] – BpTrm + SAH <br />
-	**[[3n4k]], [[3n4j]] – Trm + SAH – ''Yersinia pestis'' <br />
-	**[[1mxi]], [[1im8]] – HiTrm + SAH <br />
-	**[[4jal]] – EcTrm + SAH<br />
-
-	Trm to C5 of cytidine 38
-
-	**[[6fdf]] – fyTrm + SAH <br />
-
-	Trm to O2 of cytidine/uridine
-
-	**[[5gra]] – Trm – ''Zymomonas mobilis'' <br />
-	**[[5gmc]], [[5gmb]] – PaTrm <b />
-	**[[5gm8]] – PaTrm + SFG<br />
-
-	*'''tRNA guanine 6 N2 methyltransferase'''
-
-	**[[3tm5]] – PfTrm + SFG – ''Pyrococcvus furiosus''<br />
-	**[[3tlj]] – PfTrm + SAH<br />
-	**[[3tm4]] – PfTrm + SAM<br />
-
-	*Trm M2G/M22G10
-
-	**[[5e71]] – TkTrm – ''Thermococcvus kodakarensis''<br />
-	**[[5e72]] – TkTrm + SAM<br />
-
-	*Trm to N1 of adenine 9
-
-	**[[5a7t]], [[5a7z]] – SaTrm – ''Sulfolobus acidocaldarius''<br />
-	**[[4cnf]] – SaTrm (mutant)<br />
-	**[[5a7y]] – SaTrm + SAH<br />
-	**[[4cng]] – SaTrm (mutant) + SAH<br />
-
-	*Trm to N1 of adenine 22
-
-	**[[6q56]] – BsTrm (mutant)<br />
-	**[[6qe6]] – BsTrm + SAH<br />
-
-	*'''tRNA adenine 58 N1 methyltransferase'''
-
-	**[[5ccb]], [[5cd1]] – hTrm + SAH + tRNA-Lys<br />
-	**[[5ccx]] – hTrm + tRNA-Lys<br />
-	**[[5eqj]] – yTrm <br />
-	**[[5erg]] – yTrm + SAM<br />
-	**[[2pwy]] – TtTrm – ''Thermus thermophilus''<br />
-	**[[5c0o]], [[5c1i]] – TtTrm (mutant) + SAM<br />
-
-	*Trm to uracil 34
-
-	**[[4qnx]] – EcTrm <br />
-	**[[4qnu]], [[4qnv]] – EcTrm + SAM derivative<br />
-
-	*'''tRNA uracil 54 methyltransferase'''
-
-	**[[3bt7]] – yTrm + RNA<br />
-
-	*Trm9-TRM112
-
-	**[[5cm2]] – Trm – ''Yarrowia lipolytica''<br />
-
-	*Trm dual specificity
-
-	**[[4pl1]], [[4pl2]] – EcTrm (mutant) + SAM<br />
-	**[[6ems]], [[6emt]] – TkTrm <br />
-	**[[6emu]] – TkTrm + SAM<br />
-	**[[6emv]] –TkTrm + SAH<br />
-	}}
	== References ==		== References ==
	<references/>		<references/>
	[[Category:Topic Page]]		[[Category:Topic Page]]

Michal Harel at 09:25, 10 March 2020

Michal Harel — Tue, 10 Mar 2020 09:25:20 GMT

←Older revision		Revision as of 09:25, 10 March 2020
Line 7:		Line 7:
	== Structural highlights ==		== Structural highlights ==
	Trm <scene name='55/553109/Cv/4'>active site</scene> is located at the cleft at the subunit interface<ref>PMID:12773376</ref>. Water molecules are shown as red spheres.		Trm <scene name='55/553109/Cv/4'>active site</scene> is located at the cleft at the subunit interface<ref>PMID:12773376</ref>. Water molecules are shown as red spheres.
		+	==3D structures of tRNA methyltransferase==
		+	[[tRNA methyltransferase 3D structures]]
		+
	</StructureSection>		</StructureSection>

Line 22:		Line 25:
	*'''tRNA guanine 37 N1 methyltransferase'''		*'''tRNA guanine 37 N1 methyltransferase'''

-	**[[3knu]] – ApTrm – ''Anaplasma phagocytophilum'' <br />	+	**[[3knu]], [[6uev]] – ApTrm – ''Anaplasma phagocytophilum'' <br />
	**[[1oy5]] – AaTrm – ''Aquifex aeolicus'' <br />		**[[1oy5]] – AaTrm – ''Aquifex aeolicus'' <br />
	**[[3ief]] – Trm – ''Bartonella henselae'' <br />		**[[3ief]] – Trm – ''Bartonella henselae'' <br />
	**[[1uaj]] – HiTrm – ''Haemophilus influenzae''<br />		**[[1uaj]] – HiTrm – ''Haemophilus influenzae''<br />
	**[[3ky7]] – Trm – ''Staphylococcus aureus''<br />		**[[3ky7]] – Trm – ''Staphylococcus aureus''<br />
-	**[[3quv]] – ~~Trm~~ – ''Mycobacterium abscessus''<br />	+	**[[3quv]], [[6nvr]] – MaTrm – ''Mycobacterium abscessus''<br />
-	**[[5hjj]], [[5yac]] – ~~PaTrm~~ – ''Pyrococcus abyssi''<br />	+	**[[5hjj]], [[5yac]] – PabTrm – ''Pyrococcus abyssi''<br />
		+	**[[6jki]] – PaTrm – ''Pseudomonas aeruginosa''<br />
		+	**[[5zhi]] – MtTrm – ''Mycobacterium tuberculosis''<br />

	*Complexes of tRNA guanine 37 N1 methyltransferase		*Complexes of tRNA guanine 37 N1 methyltransferase
Line 46:		Line 51:
	**[[4gek]], [[4iwn]] – EcTrm + SAM derivative<br />		**[[4gek]], [[4iwn]] – EcTrm + SAM derivative<br />
	**[[5hr7]] – EcTrm + tRNA-Glu<br />		**[[5hr7]] – EcTrm + tRNA-Glu<br />
-	**[[5hji]], [[5hjm]] – ~~PaTrm~~ + adenosine derivative<br />	+	**[[5hji]], [[5hjm]] – PabTrm + adenosine derivative<br />
-	**[[5hjk]] – PaTrm + SAH <br />	+	**[[5hjk]] – PabTrm + SAH <br />
		+	**[[6qo2]], [[6qo3]], [[6qo4]], [[6qo6]], [[6qoa]], [[6qoc]], [[6qod]], [[6qoe]], [[6qof]], [[6qog]], [[6qoh]], [[6qoi]], [[6qoj]], [[6qok]], [[6qol]], [[6qom]], [[6qon]], [[6qoo]], [[6qop]], [[6qoq]], [[6qor]], [[6qos]], [[6qot]], [[6qou]], [[6qov]], [[6qow]], [[6qox]], [[6qqq]], [[6qqr]], [[6qqt]], [[6qqu]], [[6qqv]], [[6qqw]], [[6qqz]], [[6qr0]], [[6qr1]], [[6qr2]], [[6qr3]], [[6qr4]], [[6qr7]], [[6qr9]], [[6qra]], [[6qrc]], [[6qrd]], [[6qre]], [[6qrf]], [[6qrg]] – MaTrm + inhibitor<br />
		+	**[[6nw6]] – MaTrm + SAM <br />
		+	**[[6nw7]] – MaTrm + SAH <br />
		+	**[[6joe]], [[6jof]], [[5zhm]], [[5zhn]], [[6afk]] – PaTrm + inhibitor<br />
		+	**[[6wyq]] – PaTrm + SAM <br />
		+	**[[6wyr]] – PaTrm + SFG <br />
		+	**[[5zhj]] – MtTrm + SAH <br />
		+	**[[5zhk]], [[5zhl]] – MtTrm + inhibitor<br />
		+	**[[5wt1]] – PabTrm + tRNA + SAH <br />
		+	**[[5wt3]] – PabTrm + tRNA + methylthioadenosine<br />

	*'''tRNA guanine 46 N7 methyltransferase'''		*'''tRNA guanine 46 N7 methyltransferase'''
Line 53:		Line 68:
	**[[2vdu]], [[2vdv]] – yTrm <br />		**[[2vdu]], [[2vdv]] – yTrm <br />
	**[[1yzh]] – Trm – ''Streptococcus pneumoniae'' <br />		**[[1yzh]] – Trm – ''Streptococcus pneumoniae'' <br />
-	**[[2fca]] – ~~Trm~~ – ''Bacillus subtilis'' <br />	+	**[[2fca]] – BsTrm – ''Bacillus subtilis'' <br />
	**[[3dxx]] – EcTrm <br />		**[[3dxx]] – EcTrm <br />

Line 70:		Line 85:
	*'''tRNA cytidine 34 O2 methyltransferase'''		*'''tRNA cytidine 34 O2 methyltransferase'''

		+	**[[6jp6]] – yTrm <br />
	**[[3e5y]] – BpTrm – ''Burkholderia pseudomalllei'' <br />		**[[3e5y]] – BpTrm – ''Burkholderia pseudomalllei'' <br />
	**[[4jak]], [[4kdz]] – EcTrm <br />		**[[4jak]], [[4kdz]] – EcTrm <br />
		+	**[[6qkv]] – PaTrm <br />
		+	**[[6ahw]] – HiTrm <br />

	*Complexes of tRNA cytidine 34 O2 methyltransferase		*Complexes of tRNA cytidine 34 O2 methyltransferase

		+	**[[6jpl]] – yTrm + SAM <br />
	**[[4kgn]] – BpTrm + SAH <br />		**[[4kgn]] – BpTrm + SAH <br />
	**[[3n4k]], [[3n4j]] – Trm + SAH – ''Yersinia pestis'' <br />		**[[3n4k]], [[3n4j]] – Trm + SAH – ''Yersinia pestis'' <br />
	**[[1mxi]], [[1im8]] – HiTrm + SAH <br />		**[[1mxi]], [[1im8]] – HiTrm + SAH <br />
	**[[4jal]] – EcTrm + SAH<br />		**[[4jal]] – EcTrm + SAH<br />
		+
		+	Trm to C5 of cytidine 38
		+
		+	**[[6fdf]] – fyTrm + SAH <br />
		+
		+	Trm to O2 of cytidine/uridine
		+
		+	**[[5gra]] – Trm – ''Zymomonas mobilis'' <br />
		+	**[[5gmc]], [[5gmb]] – PaTrm <b />
		+	**[[5gm8]] – PaTrm + SFG<br />

	*'''tRNA guanine 6 N2 methyltransferase'''		*'''tRNA guanine 6 N2 methyltransferase'''
Line 97:		Line 126:
	**[[5a7y]] – SaTrm + SAH<br />		**[[5a7y]] – SaTrm + SAH<br />
	**[[4cng]] – SaTrm (mutant) + SAH<br />		**[[4cng]] – SaTrm (mutant) + SAH<br />
		+
		+	*Trm to N1 of adenine 22
		+
		+	**[[6q56]] – BsTrm (mutant)<br />
		+	**[[6qe6]] – BsTrm + SAH<br />

	*'''tRNA adenine 58 N1 methyltransferase'''		*'''tRNA adenine 58 N1 methyltransferase'''

Michal Harel at 08:46, 9 March 2020

Michal Harel — Mon, 09 Mar 2020 08:46:53 GMT

←Older revision		Revision as of 08:46, 9 March 2020
Line 25:		Line 25:
	**[[1oy5]] – AaTrm – ''Aquifex aeolicus'' <br />		**[[1oy5]] – AaTrm – ''Aquifex aeolicus'' <br />
	**[[3ief]] – Trm – ''Bartonella henselae'' <br />		**[[3ief]] – Trm – ''Bartonella henselae'' <br />
-	**[[1uaj]] – HiTrm – ''~~haemophilus~~ influenzae''<br />	+	**[[1uaj]] – HiTrm – ''Haemophilus influenzae''<br />
	**[[3ky7]] – Trm – ''Staphylococcus aureus''<br />		**[[3ky7]] – Trm – ''Staphylococcus aureus''<br />
	**[[3quv]] – Trm – ''Mycobacterium abscessus''<br />		**[[3quv]] – Trm – ''Mycobacterium abscessus''<br />
Line 39:		Line 39:
	**[[4yvi]], [[4yvj]], [[4yvk]] – HiTrm + SFG + tRNA<br />		**[[4yvi]], [[4yvj]], [[4yvk]] – HiTrm + SFG + tRNA<br />
	**[[5d9f]], [[4ypw]], [[4ypx]], [[4ypy]], [[4ypz]], [[4yq0]], [[4yq1]], [[4yq2]], [[4yq3]], [[4yq4]], [[4yq5]], [[4yq6]], [[4yq7]], [[4yq8]], [[4yq9]], [[4yqa]], [[4yqb]], [[4yqc]], [[4yqg]], [[4yqi]], [[4yqj]], [[4yqk]], [[4yql]], [[4yqn]], [[4yqo]], [[4yqp]], [[4yqq]], [[4yqr]], [[4yqs]], [[4yqt]], [[4yqd]] – HiTrm + SAM competitive<br />		**[[5d9f]], [[4ypw]], [[4ypx]], [[4ypy]], [[4ypz]], [[4yq0]], [[4yq1]], [[4yq2]], [[4yq3]], [[4yq4]], [[4yq5]], [[4yq6]], [[4yq7]], [[4yq8]], [[4yq9]], [[4yqa]], [[4yqb]], [[4yqc]], [[4yqg]], [[4yqi]], [[4yqj]], [[4yqk]], [[4yql]], [[4yqn]], [[4yqo]], [[4yqp]], [[4yqq]], [[4yqr]], [[4yqs]], [[4yqt]], [[4yqd]] – HiTrm + SAM competitive<br />
-	**[[1p9p]] – EcTrm + SAH – ''Escherichia coli'' <br />
	**[[2yx1]] – MjTrm + SFG – ''Methanocalcococcus jannaschii''<br />		**[[2yx1]] – MjTrm + SFG – ''Methanocalcococcus jannaschii''<br />
	**[[3ay0]] – MjTrm + adenosine<br />		**[[3ay0]] – MjTrm + adenosine<br />
	**[[4h3y]], [[4h3z]] – Trm + SAH – ''Burkholderia phymatum'' <br />		**[[4h3y]], [[4h3z]] – Trm + SAH – ''Burkholderia phymatum'' <br />
	**[[4ig6]] – ApTrm + SAH <br />		**[[4ig6]] – ApTrm + SAH <br />
		+	**[[1p9p]] – EcTrm + SAH – ''Escherichia coli'' <br />
	**[[4gek]], [[4iwn]] – EcTrm + SAM derivative<br />		**[[4gek]], [[4iwn]] – EcTrm + SAM derivative<br />
	**[[5hr7]] – EcTrm + tRNA-Glu<br />		**[[5hr7]] – EcTrm + tRNA-Glu<br />
Line 51:		Line 51:
	*'''tRNA guanine 46 N7 methyltransferase'''		*'''tRNA guanine 46 N7 methyltransferase'''

		+	**[[2vdu]], [[2vdv]] – yTrm <br />
	**[[1yzh]] – Trm – ''Streptococcus pneumoniae'' <br />		**[[1yzh]] – Trm – ''Streptococcus pneumoniae'' <br />
	**[[2fca]] – Trm – ''Bacillus subtilis'' <br />		**[[2fca]] – Trm – ''Bacillus subtilis'' <br />
-	**[[2vdu]], [[2vdv]] – yTrm <br />
	**[[3dxx]] – EcTrm <br />		**[[3dxx]] – EcTrm <br />

Line 100:		Line 100:
	*'''tRNA adenine 58 N1 methyltransferase'''		*'''tRNA adenine 58 N1 methyltransferase'''

-	**[[2pwy]] – TtTrm – ''Thermus thermophilus''<br />
-	**[[5c0o]], [[5c1i]] – TtTrm (mutant) + SAM<br />
-	**[[5eqj]] – yTrm <br />
-	**[[5erg]] – yTrm + SAM<br />
	**[[5ccb]], [[5cd1]] – hTrm + SAH + tRNA-Lys<br />		**[[5ccb]], [[5cd1]] – hTrm + SAH + tRNA-Lys<br />
	**[[5ccx]] – hTrm + tRNA-Lys<br />		**[[5ccx]] – hTrm + tRNA-Lys<br />
		+	**[[5eqj]] – yTrm <br />
		+	**[[5erg]] – yTrm + SAM<br />
		+	**[[2pwy]] – TtTrm – ''Thermus thermophilus''<br />
		+	**[[5c0o]], [[5c1i]] – TtTrm (mutant) + SAM<br />

	*Trm to uracil 34		*Trm to uracil 34

Alexander Berchansky at 12:59, 2 October 2019

Alexander Berchansky — Wed, 02 Oct 2019 12:59:36 GMT

←Older revision		Revision as of 12:59, 2 October 2019
Line 6:		Line 6:

	== Structural highlights ==		== Structural highlights ==
-	Trm <scene name='55/553109/Cv/3'>active site</scene> is located at the cleft at the subunit interface<ref>PMID:12773376</ref>.	+	Trm <scene name='55/553109/Cv/4'>active site</scene> is located at the cleft at the subunit interface<ref>PMID:12773376</ref>. Water molecules are shown as red spheres.
	</StructureSection>		</StructureSection>

Michal Harel at 17:43, 27 September 2018

Michal Harel — Thu, 27 Sep 2018 17:43:58 GMT

←Older revision		Revision as of 17:43, 27 September 2018
Line 1:		Line 1:
-	<StructureSection load='' size='~~450~~' side='right' caption='Structure of tRNA methyltransferase for N1 of guanine 37 complex with S-adenosylhomocysteine (PDB entry [[1uak]])' scene='55/553109/Cv/1'>	+	<StructureSection load='' size='350' side='right' caption='Structure of tRNA methyltransferase for N1 of guanine 37 complex with S-adenosylhomocysteine (PDB entry [[1uak]])' scene='55/553109/Cv/1'>
	== Function ==		== Function ==
	'''tRNA methyltransferase''' (Trm) methylates specific positions on tRNA. tRNA methylation is a post-transcription modification. The methyl donor is S-adenosylmethionine (SAM) and the product is the methylated tRNA and S-adenosylhomocysteine (SAH)<ref>PMID:25626150</ref>. S-adenosylornithine or sinefugin (SFG) is an inhibitor of the reaction.		'''tRNA methyltransferase''' (Trm) methylates specific positions on tRNA. tRNA methylation is a post-transcription modification. The methyl donor is S-adenosylmethionine (SAM) and the product is the methylated tRNA and S-adenosylhomocysteine (SAH)<ref>PMID:25626150</ref>. S-adenosylornithine or sinefugin (SFG) is an inhibitor of the reaction.
Line 123:		Line 123:

	**[[4pl1]], [[4pl2]] – EcTrm (mutant) + SAM<br />		**[[4pl1]], [[4pl2]] – EcTrm (mutant) + SAM<br />
-		+	**[[6ems]], [[6emt]] – TkTrm <br />
		+	**[[6emu]] – TkTrm + SAM<br />
		+	**[[6emv]] –TkTrm + SAH<br />
	}}		}}
	== References ==		== References ==
	<references/>		<references/>
	[[Category:Topic Page]]		[[Category:Topic Page]]

Joel L. Sussman at 04:37, 16 October 2017

Joel L. Sussman — Mon, 16 Oct 2017 04:37:25 GMT

←Older revision		Revision as of 04:37, 16 October 2017
Line 1:		Line 1:
-	<StructureSection load='~~1uak~~' size='450' side='right' caption='Structure of tRNA methyltransferase for N1 of guanine 37 complex with S-adenosylhomocysteine (PDB entry [[1uak]])' scene='55/553109/Cv/1'>	+	<StructureSection load='' size='450' side='right' caption='Structure of tRNA methyltransferase for N1 of guanine 37 complex with S-adenosylhomocysteine (PDB entry [[1uak]])' scene='55/553109/Cv/1'>
	== Function ==		== Function ==
	'''tRNA methyltransferase''' (Trm) methylates specific positions on tRNA. tRNA methylation is a post-transcription modification. The methyl donor is S-adenosylmethionine (SAM) and the product is the methylated tRNA and S-adenosylhomocysteine (SAH)<ref>PMID:25626150</ref>. S-adenosylornithine or sinefugin (SFG) is an inhibitor of the reaction.		'''tRNA methyltransferase''' (Trm) methylates specific positions on tRNA. tRNA methylation is a post-transcription modification. The methyl donor is S-adenosylmethionine (SAM) and the product is the methylated tRNA and S-adenosylhomocysteine (SAH)<ref>PMID:25626150</ref>. S-adenosylornithine or sinefugin (SFG) is an inhibitor of the reaction.

Michal Harel at 20:42, 11 October 2017

Michal Harel — Wed, 11 Oct 2017 20:42:42 GMT

←Older revision		Revision as of 20:42, 11 October 2017
Line 28:		Line 28:
	**[[3ky7]] – Trm – ''Staphylococcus aureus''<br />		**[[3ky7]] – Trm – ''Staphylococcus aureus''<br />
	**[[3quv]] – Trm – ''Mycobacterium abscessus''<br />		**[[3quv]] – Trm – ''Mycobacterium abscessus''<br />
		+	**[[5hjj]], [[5yac]] – PaTrm – ''Pyrococcus abyssi''<br />

	*Complexes of tRNA guanine 37 N1 methyltransferase		*Complexes of tRNA guanine 37 N1 methyltransferase
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	**[[4gek]], [[4iwn]] – EcTrm + SAM derivative<br />		**[[4gek]], [[4iwn]] – EcTrm + SAM derivative<br />
	**[[5hr7]] – EcTrm + tRNA-Glu<br />		**[[5hr7]] – EcTrm + tRNA-Glu<br />
		+	**[[5hji]], [[5hjm]] – PaTrm + adenosine derivative<br />
		+	**[[5hjk]] – PaTrm + SAH <br />

	*'''tRNA guanine 46 N7 methyltransferase'''		*'''tRNA guanine 46 N7 methyltransferase'''

Michal Harel at 20:28, 11 October 2017

Michal Harel — Wed, 11 Oct 2017 20:28:32 GMT

←Older revision		Revision as of 20:28, 11 October 2017
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	*Trm M2G/M22G10		*Trm M2G/M22G10

-	**[[5e71]] – TkTrm – Thermococcvus kodakarensis<br />	+	**[[5e71]] – TkTrm – ''Thermococcvus kodakarensis''<br />
	**[[5e72]] – TkTrm + SAM<br />		**[[5e72]] – TkTrm + SAM<br />

	*Trm to N1 of adenine 9		*Trm to N1 of adenine 9

-	**[[5a7t]], [[5a7z]] – SaTrm – Sulfolobus acidocaldarius<br />	+	**[[5a7t]], [[5a7z]] – SaTrm – ''Sulfolobus acidocaldarius''<br />
	**[[4cnf]] – SaTrm (mutant)<br />		**[[4cnf]] – SaTrm (mutant)<br />
	**[[5a7y]] – SaTrm + SAH<br />		**[[5a7y]] – SaTrm + SAH<br />

Alexander Berchansky at 12:12, 6 April 2017

Alexander Berchansky — Thu, 06 Apr 2017 12:12:28 GMT

←Older revision		Revision as of 12:12, 6 April 2017
Line 6:		Line 6:

	== Structural highlights ==		== Structural highlights ==
-	Trm active site is located at the cleft at the subunit interface<ref>PMID:12773376</ref>.	+	Trm <scene name='55/553109/Cv/3'>active site</scene> is located at the cleft at the subunit interface<ref>PMID:12773376</ref>.
	</StructureSection>		</StructureSection>

Alexander Berchansky at 11:59, 6 April 2017

Alexander Berchansky — Thu, 06 Apr 2017 11:59:21 GMT

←Older revision		Revision as of 11:59, 6 April 2017
Line 1:		Line 1:
-	<StructureSection load='1uak' size='450' side='right' caption='Structure of tRNA methyltransferase for N1 of guanine 37 complex with S-adenosylhomocysteine (PDB entry [[1uak]])' scene=''>	+	<StructureSection load='1uak' size='450' side='right' caption='Structure of tRNA methyltransferase for N1 of guanine 37 complex with S-adenosylhomocysteine (PDB entry [[1uak]])' scene='55/553109/Cv/1'>
	== Function ==		== Function ==
	'''tRNA methyltransferase''' (Trm) methylates specific positions on tRNA. tRNA methylation is a post-transcription modification. The methyl donor is S-adenosylmethionine (SAM) and the product is the methylated tRNA and S-adenosylhomocysteine (SAH)<ref>PMID:25626150</ref>. S-adenosylornithine or sinefugin (SFG) is an inhibitor of the reaction.		'''tRNA methyltransferase''' (Trm) methylates specific positions on tRNA. tRNA methylation is a post-transcription modification. The methyl donor is S-adenosylmethionine (SAM) and the product is the methylated tRNA and S-adenosylhomocysteine (SAH)<ref>PMID:25626150</ref>. S-adenosylornithine or sinefugin (SFG) is an inhibitor of the reaction.