Terminase - Revision history

Michal Harel at 08:52, 19 April 2022

Michal Harel — Tue, 19 Apr 2022 08:52:19 GMT

←Older revision		Revision as of 08:52, 19 April 2022
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	[[Terminase 3D Structures ]]		[[Terminase 3D Structures ]]
	</StructureSection>		</StructureSection>
-	== 3D Structures of terminase ==
-
-	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
-	{{#tree:id=OrganizedByTopic\|openlevels=0\|
-
-	*Terminase small subunit
-
-	**[[3n4p]], [[3n4q]] – HvTer nuclease domain – Herpesvirus<br />
-	**[[6ey7]] – HvTer nuclease domain + inhibitor<br />
-	**[[3hef]], [[7lxs]] – Ter – Bacteriophage T7 <br />
-	**[[4zc3]], [[2cmp]] – Sf6Ter nuclease domain – Bacteriophage Sf6 <br />
-	**[[4dyc]], [[4dzj]], [[4dzp]] – Sf6Ter nuclease domain (mutant) <br />
-	**[[3zqp]], [[3zqm]], [[3zqn]], [[3zqo]] – Sf6Ter oligomerization domain <br />
-	**[[3zqq]], [[4dyq]], [[4dyr]] – Sf6Ter <br />
-	**[[1j9i]] – Ter nuclease domain – Bacteriophage λ - NMR <br />
-	**[[4xvn]] – G20cTer nuclease domain – Bacteriophage G20c <br />
-	**[[3p9a]] – Ter – Bacteriophage P22 <br />
-	**[[6v1i]] – Ter – Thermus virus – Cryo EM<br />
-	**[[6ejq]] – TpTer – Thermus phage<br />
-	**[[7lw0]] – Ter – ''E. coli'' virus λ <br />
-	**[[6hn7]] – Ter + RPPC <br />
-	**[[7joq]] – Ter – Pseudomonas phage NV1<br />
-	**[[6w7t]] – Ter – Pseudomonas phage PaP3<br />
-	**[[7lwr]] – Ter – Enterobacteria phage P21<br />
-
-	*Terminase large subunit
-
-	**[[4dkw]] – Ter nuclease domain – Bacteriophage P22<br />
-	**[[4bij]], [[4bil]] – T7Ter – Bacteriophage T7 – Cryo EM<br />
-	**[[4idh]] – T7Ter <br />
-	**[[2wbn]], [[2wc9]] – Ter nuclease domain – Bacteriophage Spp1 <br />
-	**[[3c6a]], [[3c6h]] – Ter nuclease domain – Bacteriophage Rb49 <br />
-	**[[5c10]], [[5c12]] – Sf6Ter nuclease domain <br />
-	**[[5c2d]], [[5c15]] – Sf6Ter nuclease domain (mutant)<br />
-	**[[5m1f]], [[5m1k]], [[5m1n]], [[5m1o]], [[5m1p]], [[5m1q]] – G20cTer <br />
-	**[[5oe8]], [[5oee]], [[5oe9]] – D6eTer – Bacteriophage D6e <br />
-	**[[5tge]] – P74Ter nuclease domain – Thermus phage P74 <br />
-	**[[4zni]], [[4znk]] – P74Ter ATPase domain <br />
-	**[[4znj]] – P74Ter ATPase domain (mutant)<br />
-	**[[6z6d]], [[6z6e]] – Ter – ''E. coli'' virus H97 <br />
-
-	*Terminase large subunit complex
-
-	**[[4iee]], [[4ife]] – Sf6Ter + ATP<br />
-	**[[4iei]] – Sf6Ter + ADP<br />
-	**[[5c2f]] – Sf6Ter nuclease domain (mutant) + antifungal drug<br />
-	**[[5oea]] – D6eTer + ATP <br />
-	**[[5oeb]] – D6eTer + ADP <br />
-	**[[6vi1]], [[6xmi]] – Ter + antibody – Salmonella phage P22<br />
-	**[[4znl]] – P74Ter ATPase domain + ADP <br />
-	**[[7ks4]] – TpTer ATPase domain + ADP <br />
-
-	*Tripartite terminase
-
-	**[[6m5s]], [[6m5r]] – HvTer TRM1+TRM2+TRM3 – Cryo EM<br />
-	**[[6m5u]], [[6m5v]] – HvTer TRM1+TRM2+TRM3 + ADP – Cryo EM<br />
-	**[[6m5t]] – HvTer TRM3 – Cryo EM<br />
-	}}

	== References ==		== References ==
	<references/>		<references/>
	[[Category:Topic Page]]		[[Category:Topic Page]]

Michal Harel at 08:50, 19 April 2022

Michal Harel — Tue, 19 Apr 2022 08:50:11 GMT

←Older revision		Revision as of 08:50, 19 April 2022
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	The large subunit of Ter is composed of an <scene name='77/775254/Cv/5'>N-terminal ATPase domain, a linker region and a C-terminal nuclease domain</scene>. ATP binds to the protein in a <scene name='77/775254/Cv/6'>groove between the ATPase domain and the linker region</scene><ref>PMID:23630261</ref>.		The large subunit of Ter is composed of an <scene name='77/775254/Cv/5'>N-terminal ATPase domain, a linker region and a C-terminal nuclease domain</scene>. ATP binds to the protein in a <scene name='77/775254/Cv/6'>groove between the ATPase domain and the linker region</scene><ref>PMID:23630261</ref>.
		+	== 3D Structures of terminase ==
		+	[[Terminase 3D Structures ]]
	</StructureSection>		</StructureSection>
	== 3D Structures of terminase ==		== 3D Structures of terminase ==
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	**[[3n4p]], [[3n4q]] – HvTer nuclease domain – Herpesvirus<br />		**[[3n4p]], [[3n4q]] – HvTer nuclease domain – Herpesvirus<br />
	**[[6ey7]] – HvTer nuclease domain + inhibitor<br />		**[[6ey7]] – HvTer nuclease domain + inhibitor<br />
-	**[[3hef]] – Ter – Bacteriophage T7 <br />	+	**[[3hef]], [[7lxs]] – Ter – Bacteriophage T7 <br />
	**[[4zc3]], [[2cmp]] – Sf6Ter nuclease domain – Bacteriophage Sf6 <br />		**[[4zc3]], [[2cmp]] – Sf6Ter nuclease domain – Bacteriophage Sf6 <br />
	**[[4dyc]], [[4dzj]], [[4dzp]] – Sf6Ter nuclease domain (mutant) <br />		**[[4dyc]], [[4dzj]], [[4dzp]] – Sf6Ter nuclease domain (mutant) <br />
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	**[[3p9a]] – Ter – Bacteriophage P22 <br />		**[[3p9a]] – Ter – Bacteriophage P22 <br />
	**[[6v1i]] – Ter – Thermus virus – Cryo EM<br />		**[[6v1i]] – Ter – Thermus virus – Cryo EM<br />
-	**[[6ejq]] – ~~Ter~~ – Thermus phage<br />	+	**[[6ejq]] – TpTer – Thermus phage<br />
-	**[[~~6hn7~~]] – Ter ~~+ RPPC~~ – ''E. coli'' virus λ <br />	+	**[[7lw0]] – Ter – ''E. coli'' virus λ <br />
		+	**[[6hn7]] – Ter + RPPC <br />
	**[[7joq]] – Ter – Pseudomonas phage NV1<br />		**[[7joq]] – Ter – Pseudomonas phage NV1<br />
	**[[6w7t]] – Ter – Pseudomonas phage PaP3<br />		**[[6w7t]] – Ter – Pseudomonas phage PaP3<br />
		+	**[[7lwr]] – Ter – Enterobacteria phage P21<br />

	*Terminase large subunit		*Terminase large subunit
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	**[[4zni]], [[4znk]] – P74Ter ATPase domain <br />		**[[4zni]], [[4znk]] – P74Ter ATPase domain <br />
	**[[4znj]] – P74Ter ATPase domain (mutant)<br />		**[[4znj]] – P74Ter ATPase domain (mutant)<br />
-	**[[~~4znl~~]] – ~~P74Ter ATPase domain + ADP~~ <br />	+	**[[6z6d]], [[6z6e]] – Ter – ''E. coli'' virus H97 <br />

	*Terminase large subunit complex		*Terminase large subunit complex
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	**[[5oeb]] – D6eTer + ADP <br />		**[[5oeb]] – D6eTer + ADP <br />
	**[[6vi1]], [[6xmi]] – Ter + antibody – Salmonella phage P22<br />		**[[6vi1]], [[6xmi]] – Ter + antibody – Salmonella phage P22<br />
		+	**[[4znl]] – P74Ter ATPase domain + ADP <br />
		+	**[[7ks4]] – TpTer ATPase domain + ADP <br />

	*Tripartite terminase		*Tripartite terminase

Michal Harel at 08:37, 23 November 2020

Michal Harel — Mon, 23 Nov 2020 08:37:54 GMT

←Older revision		Revision as of 08:37, 23 November 2020
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	== Function ==		== Function ==

-	'''Terminase''' (Ter) is a key component of the DNA packaging machine found in bacteriophages and herpesviruses. The Ter complex is comprised of a '''small Ter subunit''' which is a recognition subunit and a '''large Ter subunit''' which is an endonuclease/translocase subunit <ref>PMID:22'''297528</ref>. The nuclease activity of the large Ter subunit is stimulated by ATP. The''' tripartite terminase complex of herpesvirus which ~~contins~~ 3 subunits (TRM1, TRM2 and TRM3), is found in the cytoplasm of infected cells and uses the cell's import machinery to enter the nucleus<ref>PMID:27033706</ref>. '''TRM3''' has RNase H-like activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genome<ref>PMID:24155374</ref>.	+	'''Terminase''' (Ter) is a key component of the DNA packaging machine found in bacteriophages and herpesviruses. The Ter complex is comprised of a '''small Ter subunit''' which is a recognition subunit and a '''large Ter subunit''' which is an endonuclease/translocase subunit <ref>PMID:22'''297528</ref>. The nuclease activity of the large Ter subunit is stimulated by ATP. The '''tripartite terminase''' complex of herpesvirus which contains 3 subunits (TRM1, TRM2 and TRM3), is found in the cytoplasm of infected cells and uses the cell's import machinery to enter the nucleus<ref>PMID:27033706</ref>. '''TRM3''' has RNase H-like activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genome<ref>PMID:24155374</ref>.

	== Structural highlights ==		== Structural highlights ==

Michal Harel at 08:37, 23 November 2020

Michal Harel — Mon, 23 Nov 2020 08:37:04 GMT

←Older revision		Revision as of 08:37, 23 November 2020
Line 4:		Line 4:
	== Function ==		== Function ==

-	'''Terminase''' (Ter) is a key component of the DNA packaging machine found in bacteriophages and herpesviruses. The Ter complex is comprised of a small Ter subunit which is a recognition subunit and a large Ter subunit which is an endonuclease/translocase subunit <ref>PMID:~~22297528~~</ref>. The nuclease activity of the large Ter subunit is stimulated by ATP.	+	'''Terminase''' (Ter) is a key component of the DNA packaging machine found in bacteriophages and herpesviruses. The Ter complex is comprised of a '''small Ter subunit''' which is a recognition subunit and a '''large Ter subunit''' which is an endonuclease/translocase subunit <ref>PMID:22'''297528</ref>. The nuclease activity of the large Ter subunit is stimulated by ATP. The''' tripartite terminase complex of herpesvirus which contins 3 subunits (TRM1, TRM2 and TRM3), is found in the cytoplasm of infected cells and uses the cell's import machinery to enter the nucleus<ref>PMID:27033706</ref>. '''TRM3''' has RNase H-like activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genome<ref>PMID:24155374</ref>.

	== Structural highlights ==		== Structural highlights ==

Michal Harel at 08:19, 23 November 2020

Michal Harel — Mon, 23 Nov 2020 08:19:31 GMT

←Older revision		Revision as of 08:19, 23 November 2020
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	{{#tree:id=OrganizedByTopic\|openlevels=0\|		{{#tree:id=OrganizedByTopic\|openlevels=0\|

-	*~~Small terminase~~	+	*Terminase small subunit

	**[[3n4p]], [[3n4q]] – HvTer nuclease domain – Herpesvirus<br />		**[[3n4p]], [[3n4q]] – HvTer nuclease domain – Herpesvirus<br />
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	**[[6ejq]] – Ter – Thermus phage<br />		**[[6ejq]] – Ter – Thermus phage<br />
	**[[6hn7]] – Ter + RPPC – ''E. coli'' virus λ <br />		**[[6hn7]] – Ter + RPPC – ''E. coli'' virus λ <br />
		+	**[[7joq]] – Ter – Pseudomonas phage NV1<br />
		+	**[[6w7t]] – Ter – Pseudomonas phage PaP3<br />

-	*~~Large terminase~~	+	*Terminase large subunit

	**[[4dkw]] – Ter nuclease domain – Bacteriophage P22<br />		**[[4dkw]] – Ter nuclease domain – Bacteriophage P22<br />
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	**[[4znl]] – P74Ter ATPase domain + ADP <br />		**[[4znl]] – P74Ter ATPase domain + ADP <br />

-	*~~Large terminase~~ complex	+	*Terminase large subunit complex

	**[[4iee]], [[4ife]] – Sf6Ter + ATP<br />		**[[4iee]], [[4ife]] – Sf6Ter + ATP<br />
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	**[[5oea]] – D6eTer + ATP <br />		**[[5oea]] – D6eTer + ATP <br />
	**[[5oeb]] – D6eTer + ADP <br />		**[[5oeb]] – D6eTer + ADP <br />
		+	**[[6vi1]], [[6xmi]] – Ter + antibody – Salmonella phage P22<br />
		+
		+	*Tripartite terminase
		+
		+	**[[6m5s]], [[6m5r]] – HvTer TRM1+TRM2+TRM3 – Cryo EM<br />
		+	**[[6m5u]], [[6m5v]] – HvTer TRM1+TRM2+TRM3 + ADP – Cryo EM<br />
		+	**[[6m5t]] – HvTer TRM3 – Cryo EM<br />
	}}		}}

Michal Harel at 11:12, 13 February 2020

Michal Harel — Thu, 13 Feb 2020 11:12:05 GMT

←Older revision		Revision as of 11:12, 13 February 2020
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	*Small terminase		*Small terminase

-	**[[3n4p]], [[3n4q]] – ~~Ter~~ nuclease domain – Herpesvirus<br />	+	**[[3n4p]], [[3n4q]] – HvTer nuclease domain – Herpesvirus<br />
		+	**[[6ey7]] – HvTer nuclease domain + inhibitor<br />
	**[[3hef]] – Ter – Bacteriophage T7 <br />		**[[3hef]] – Ter – Bacteriophage T7 <br />
	**[[4zc3]], [[2cmp]] – Sf6Ter nuclease domain – Bacteriophage Sf6 <br />		**[[4zc3]], [[2cmp]] – Sf6Ter nuclease domain – Bacteriophage Sf6 <br />
Line 26:		Line 27:
	**[[4xvn]] – G20cTer nuclease domain – Bacteriophage G20c <br />		**[[4xvn]] – G20cTer nuclease domain – Bacteriophage G20c <br />
	**[[3p9a]] – Ter – Bacteriophage P22 <br />		**[[3p9a]] – Ter – Bacteriophage P22 <br />
		+	**[[6v1i]] – Ter – Thermus virus – Cryo EM<br />
		+	**[[6ejq]] – Ter – Thermus phage<br />
		+	**[[6hn7]] – Ter + RPPC – ''E. coli'' virus λ <br />

	*Large terminase		*Large terminase

Alexander Berchansky at 12:53, 19 September 2019

Alexander Berchansky — Thu, 19 Sep 2019 12:53:55 GMT

←Older revision		Revision as of 12:53, 19 September 2019
Line 8:		Line 8:
	== Structural highlights ==		== Structural highlights ==

-	The large subunit of Ter is composed of an <scene name='77/775254/Cv/2'>N-terminal ATPase domain, a linker region and a C-terminal nuclease domain</scene>. ATP binds to the protein in a <scene name='77/775254/Cv/4'>groove between the ATPase domain and the linker region</scene><ref>PMID:23630261</ref>.	+	The large subunit of Ter is composed of an <scene name='77/775254/Cv/5'>N-terminal ATPase domain, a linker region and a C-terminal nuclease domain</scene>. ATP binds to the protein in a <scene name='77/775254/Cv/6'>groove between the ATPase domain and the linker region</scene><ref>PMID:23630261</ref>.
	</StructureSection>		</StructureSection>
	== 3D Structures of terminase ==		== 3D Structures of terminase ==

Michal Harel at 21:56, 24 September 2018

Michal Harel — Mon, 24 Sep 2018 21:56:09 GMT

←Older revision		Revision as of 21:56, 24 September 2018
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-	<StructureSection load='4ife' size='~~450~~' side='right' caption='Terminase large subunit complex with ATP (PDB code [[4ife]])' scene='77/775254/Cv/1'>	+	<StructureSection load='4ife' size='350' side='right' caption='Terminase large subunit complex with ATP (PDB code [[4ife]])' scene='77/775254/Cv/1'>

Alexander Berchansky at 14:10, 6 December 2017

Alexander Berchansky — Wed, 06 Dec 2017 14:10:41 GMT

←Older revision		Revision as of 14:10, 6 December 2017
Line 8:		Line 8:
	== Structural highlights ==		== Structural highlights ==

-	The large subunit of Ter is composed of an N-terminal ATPase domain, a linker region and a C-terminal nuclease domain. ATP binds to the protein in a groove between the ATPase domain and the linker region<ref>PMID:23630261</ref>.	+	The large subunit of Ter is composed of an <scene name='77/775254/Cv/2'>N-terminal ATPase domain, a linker region and a C-terminal nuclease domain</scene>. ATP binds to the protein in a <scene name='77/775254/Cv/4'>groove between the ATPase domain and the linker region</scene><ref>PMID:23630261</ref>.
	</StructureSection>		</StructureSection>
	== 3D Structures of terminase ==		== 3D Structures of terminase ==

Alexander Berchansky at 13:47, 6 December 2017

Alexander Berchansky — Wed, 06 Dec 2017 13:47:20 GMT

←Older revision		Revision as of 13:47, 6 December 2017
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-	<StructureSection load='4ife' size='~~340~~' side='right' caption='Terminase large subunit complex with ATP (PDB code [[4ife]])' scene=''>	+	<StructureSection load='4ife' size='450' side='right' caption='Terminase large subunit complex with ATP (PDB code [[4ife]])' scene='77/775254/Cv/1'>