UDP-galactose 4-epimerase - Revision history

Michal Harel at 09:51, 11 March 2024

Michal Harel — Mon, 11 Mar 2024 09:51:12 GMT

←Older revision		Revision as of 09:51, 11 March 2024
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	==3D structures of UDP-galactose 4 epimerase==		==3D structures of UDP-galactose 4 epimerase==

-	~~Updated on {{REVISIONDAY2}}~~-~~{{MONTHNAME\|{{REVISIONMONTH}}}}~~-~~{{REVISIONYEAR}}~~	+	[[UDP-galactose 4-epimerase 3D structures]]
-	~~{{#tree:id=OrganizedByTopic\|openlevels=0\|~~	+

-	*UDP-galactose 4-epimerase with no ligand
-
-	**[[1ek5]] - hGalE + NAD - human<br />
-	**[[2c20]] - GalE + NAD – ''Bacillus anthracis''<br />
-	**[[2p5u]], [[2p5y]] - GalE + NAD – ''Thermus thermophilus''<br />
-	**[[3enk]] - GalE + NAD – ''Burkholderia pseudo mallei''<br />
-	**[[3icp]], [[3ko8]] - PcGalE + NAD – ''Pyrobaculum calidifontis''<br />
-
-	*UDP-galactose 4-epimerase complexes
-
-	**[[1ek6]] - hGalE + NAD + UDP-glucose<br />
-	**[[1hzj]] - hGalE + NAD + UDP-glucosamine<br />
-	**[[1i3k]] - hGalE (mutant) + NAD + UDP-glucose<br />
-	**[[1i3l]], [[2cnb]] - hGalE (mutant) + NAD + UDP-galactose<br />
-	**[[1i3m]], [[1i3n]] - hGalE (mutant) + NAD + UDP-acetylglucosamine<br />
-	**[[1nah]], [[1nai]] – EcGalE + NAD + UDP – ''Escherichia coli''<br />
-	**[[1gy8]], [[5gy7]] - EcGalE (mutant) + NAD + UDP <br />
-	**[[1xel]], [[1udb]], [[1a9y]] - EcGalE + NAD + UDP-glucose<br />
-	**[[1a9z]], [[1lrl]] - EcGalE (mutant) + NAD + UDP-glucose<br />
-	**[[1uda]] - EcGalE + NAD + UDP-galactose<br />
-	**[[1lrj]] - EcGalE + NAD + UDP-acetylglucosamine<br />
-	**[[1lrj]], [[1lrk]] - EcGalE (mutant) + NAD + UDP-acetylglucosamine<br />
-	**[[1udc]] - EcGalE + NAD + UDP-mannose<br />
-	**[[1kvr]], [[1kvs]], [[1kvt]], [[1kvu]], [[1kvq]] – EcGalE (mutant) + NAD + UDP-phenol<br />
-	**[[3aw9]] - PcGalE (mutant) + NAD + UDP-galactopyranose <br />
-	**[[4lis]] – GalE + NAD + UDP + UPG – ''Aspergillus nidulans''<br />
-	**[[6k0g]] – BlGalE + NAD + UDP – ''Bifidobacterium longum''<br />
-	**[[6k0h]] – BlGalE + NAD + UDP-GlcNAc<br />
-	**[[6k0i]] – BlGalE + NAD + UDP-glucose<br />
-
-	}}
	==References==		==References==
	<references />		<references />
		+	</StructureSection>
	[[Category:Topic Page]]		[[Category:Topic Page]]

Michal Harel at 09:59, 19 March 2020

Michal Harel — Thu, 19 Mar 2020 09:59:18 GMT

←Older revision		Revision as of 09:59, 19 March 2020
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	*UDP-galactose 4-epimerase complexes		*UDP-galactose 4-epimerase complexes

		+	**[[1ek6]] - hGalE + NAD + UDP-glucose<br />
		+	**[[1hzj]] - hGalE + NAD + UDP-glucosamine<br />
		+	**[[1i3k]] - hGalE (mutant) + NAD + UDP-glucose<br />
		+	**[[1i3l]], [[2cnb]] - hGalE (mutant) + NAD + UDP-galactose<br />
		+	**[[1i3m]], [[1i3n]] - hGalE (mutant) + NAD + UDP-acetylglucosamine<br />
	**[[1nah]], [[1nai]] – EcGalE + NAD + UDP – ''Escherichia coli''<br />		**[[1nah]], [[1nai]] – EcGalE + NAD + UDP – ''Escherichia coli''<br />
	**[[1gy8]], [[5gy7]] - EcGalE (mutant) + NAD + UDP <br />		**[[1gy8]], [[5gy7]] - EcGalE (mutant) + NAD + UDP <br />
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	**[[1udc]] - EcGalE + NAD + UDP-mannose<br />		**[[1udc]] - EcGalE + NAD + UDP-mannose<br />
	**[[1kvr]], [[1kvs]], [[1kvt]], [[1kvu]], [[1kvq]] – EcGalE (mutant) + NAD + UDP-phenol<br />		**[[1kvr]], [[1kvs]], [[1kvt]], [[1kvu]], [[1kvq]] – EcGalE (mutant) + NAD + UDP-phenol<br />
-	**[[2udp]] - EcGalE + NAD + UDP-phenol<br />
-	**[[1ek6]] - hGalE + NAD + UDP-glucose<br />
-	**[[1hzj]] - hGalE + NAD + UDP-glucosamine<br />
-	**[[1i3k]] - hGalE (mutant) + NAD + UDP-glucose<br />
-	**[[1i3l]], [[2cnb]] - hGalE (mutant) + NAD + UDP-galactose<br />
-	**[[1i3m]], [[1i3n]] - hGalE (mutant) + NAD + UDP-acetylglucosamine<br />
	**[[3aw9]] - PcGalE (mutant) + NAD + UDP-galactopyranose <br />		**[[3aw9]] - PcGalE (mutant) + NAD + UDP-galactopyranose <br />
	**[[4lis]] – GalE + NAD + UDP + UPG – ''Aspergillus nidulans''<br />		**[[4lis]] – GalE + NAD + UDP + UPG – ''Aspergillus nidulans''<br />
		+	**[[6k0g]] – BlGalE + NAD + UDP – ''Bifidobacterium longum''<br />
		+	**[[6k0h]] – BlGalE + NAD + UDP-GlcNAc<br />
		+	**[[6k0i]] – BlGalE + NAD + UDP-glucose<br />
		+
	}}		}}
	==References==		==References==
	<references />		<references />
	[[Category:Topic Page]]		[[Category:Topic Page]]

Alexander Berchansky at 12:21, 7 October 2019

Alexander Berchansky — Mon, 07 Oct 2019 12:21:09 GMT

←Older revision		Revision as of 12:21, 7 October 2019
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	== Structural highlights ==		== Structural highlights ==
-	GalE active site contains the <scene name='57/572287/Cv/4'>substrate UDP-galactose</scene> (UDP-gal) and the <scene name='57/572287/Cv/6'>cofactor NAD</scene>. Water molecules shown as red spheres. <scene name='57/572287/Cv/8'>Tyrosine residue is the catalytic base</scene> which produces the ketose intermediate<ref>PMID:16946458</ref>.	+	GalE active site contains the <scene name='57/572287/Cv/9'>substrate UDP-galactose</scene> (UDP-gal) and the <scene name='57/572287/Cv/10'>cofactor NAD</scene>. Water molecules are shown as red spheres. <scene name='57/572287/Cv/11'>Tyrosine residue is the catalytic base</scene> which produces the ketose intermediate<ref>PMID:16946458</ref>.
	</StructureSection>		</StructureSection>

Michal Harel at 19:34, 4 October 2018

Michal Harel — Thu, 04 Oct 2018 19:34:28 GMT

←Older revision		Revision as of 19:34, 4 October 2018
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-	<StructureSection load='2cnb' size='~~450~~' side='right' caption='Structure of UDP-galactose 4 epimerase complex with NAD and UDP-galactose (PDB entry [[2cnb]])' scene='57/572287/Cv/1' pspeed='8'>	+	<StructureSection load='2cnb' size='350' side='right' caption='Structure of UDP-galactose 4 epimerase complex with NAD and UDP-galactose (PDB entry [[2cnb]])' scene='57/572287/Cv/1' pspeed='8'>
	== Function ==		== Function ==
	'''UDP-galactose 4-epimerase''' (GalE) reversibly converts UDP-galactose to UDP-glucose. GalE catalyzes the last step in galactose metabolism<ref>PMID:12534808</ref>. GalE uses NAD+ as a cofactor.		'''UDP-galactose 4-epimerase''' (GalE) reversibly converts UDP-galactose to UDP-glucose. GalE catalyzes the last step in galactose metabolism<ref>PMID:12534808</ref>. GalE uses NAD+ as a cofactor.

Alexander Berchansky at 12:38, 23 March 2017

Alexander Berchansky — Thu, 23 Mar 2017 12:38:28 GMT

←Older revision		Revision as of 12:38, 23 March 2017
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	== Structural highlights ==		== Structural highlights ==
-	GalE active site contains the <scene name='57/572287/Cv/4'>substrate UDP-galactose</scene> (UDP-gal) and the <scene name='57/572287/Cv/6'>cofactor NAD</scene>. Water molecules shown as red spheres.	+	GalE active site contains the <scene name='57/572287/Cv/4'>substrate UDP-galactose</scene> (UDP-gal) and the <scene name='57/572287/Cv/6'>cofactor NAD</scene>. Water molecules shown as red spheres. <scene name='57/572287/Cv/8'>Tyrosine residue is the catalytic base</scene> which produces the ketose intermediate<ref>PMID:16946458</ref>.
-	*<scene name='57/572287/Cv/7'>Tyrosine residue is the catalytic base</scene> which produces the ketose intermediate<ref>PMID:16946458</ref>.	+
	</StructureSection>		</StructureSection>

Alexander Berchansky at 12:31, 23 March 2017

Alexander Berchansky — Thu, 23 Mar 2017 12:31:15 GMT

←Older revision		Revision as of 12:31, 23 March 2017
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	== Structural highlights ==		== Structural highlights ==
-	GalE active site contains the ~~substrate~~ <scene name='57/572287/Cv/4'>UDP-galactose</scene> (UDP-gal) and the cofactor NAD. Tyrosine residue is the catalytic base which produces the ketose intermediate<ref>PMID:16946458</ref>.	+	GalE active site contains the <scene name='57/572287/Cv/4'>substrate UDP-galactose</scene> (UDP-gal) and the <scene name='57/572287/Cv/6'>cofactor NAD</scene>. Water molecules shown as red spheres.
		+	*<scene name='57/572287/Cv/7'>Tyrosine residue is the catalytic base</scene> which produces the ketose intermediate<ref>PMID:16946458</ref>.
	</StructureSection>		</StructureSection>

Alexander Berchansky at 12:12, 23 March 2017

Alexander Berchansky — Thu, 23 Mar 2017 12:12:33 GMT

←Older revision		Revision as of 12:12, 23 March 2017
Line 7:		Line 7:

	== Structural highlights ==		== Structural highlights ==
-	GalE active site contains the substrate UDP-galactose (UDP-gal) and the cofactor NAD. Tyrosine residue is the catalytic base which produces the ketose intermediate<ref>PMID:16946458</ref>.	+	GalE active site contains the substrate <scene name='57/572287/Cv/4'>UDP-galactose</scene> (UDP-gal) and the cofactor NAD. Tyrosine residue is the catalytic base which produces the ketose intermediate<ref>PMID:16946458</ref>.
	</StructureSection>		</StructureSection>

Alexander Berchansky at 11:38, 23 March 2017

Alexander Berchansky — Thu, 23 Mar 2017 11:38:05 GMT

←Older revision		Revision as of 11:38, 23 March 2017
Line 1:		Line 1:
-	<StructureSection load='2cnb' size='~~350~~' side='right' caption='Structure of UDP-galactose 4 epimerase complex with NAD and UDP-galactose (PDB entry [[2cnb]])' scene=''>	+	<StructureSection load='2cnb' size='450' side='right' caption='Structure of UDP-galactose 4 epimerase complex with NAD and UDP-galactose (PDB entry [[2cnb]])' scene='57/572287/Cv/1' pspeed='8'>
	== Function ==		== Function ==
	'''UDP-galactose 4-epimerase''' (GalE) reversibly converts UDP-galactose to UDP-glucose. GalE catalyzes the last step in galactose metabolism<ref>PMID:12534808</ref>. GalE uses NAD+ as a cofactor.		'''UDP-galactose 4-epimerase''' (GalE) reversibly converts UDP-galactose to UDP-glucose. GalE catalyzes the last step in galactose metabolism<ref>PMID:12534808</ref>. GalE uses NAD+ as a cofactor.

Michal Harel at 10:42, 5 December 2016

Michal Harel — Mon, 05 Dec 2016 10:42:11 GMT

←Older revision		Revision as of 10:42, 5 December 2016
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	**[[1nah]], [[1nai]] – EcGalE + NAD + UDP – ''Escherichia coli''<br />		**[[1nah]], [[1nai]] – EcGalE + NAD + UDP – ''Escherichia coli''<br />
-	**[[1gy8]] - EcGalE (mutant) + NAD + UDP <br />	+	**[[1gy8]], [[5gy7]] - EcGalE (mutant) + NAD + UDP <br />
	**[[1xel]], [[1udb]], [[1a9y]] - EcGalE + NAD + UDP-glucose<br />		**[[1xel]], [[1udb]], [[1a9y]] - EcGalE + NAD + UDP-glucose<br />
	**[[1a9z]], [[1lrl]] - EcGalE (mutant) + NAD + UDP-glucose<br />		**[[1a9z]], [[1lrl]] - EcGalE (mutant) + NAD + UDP-glucose<br />
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	**[[1i3m]], [[1i3n]] - hGalE (mutant) + NAD + UDP-acetylglucosamine<br />		**[[1i3m]], [[1i3n]] - hGalE (mutant) + NAD + UDP-acetylglucosamine<br />
	**[[3aw9]] - PcGalE (mutant) + NAD + UDP-galactopyranose <br />		**[[3aw9]] - PcGalE (mutant) + NAD + UDP-galactopyranose <br />
		+	**[[4lis]] – GalE + NAD + UDP + UPG – ''Aspergillus nidulans''<br />
	}}		}}
	==References==		==References==
	<references />		<references />
	[[Category:Topic Page]]		[[Category:Topic Page]]

Michal Harel at 08:26, 5 December 2016

Michal Harel — Mon, 05 Dec 2016 08:26:39 GMT

←Older revision		Revision as of 08:26, 5 December 2016
Line 1:		Line 1:
-	<StructureSection load='2cnb' size='350' side='right' caption='Structure of UDP-galactose 4 epimerase complex with NAD, UDP ~~(stick model), PEG and Na+1 (purple) ions~~ (PDB entry [[~~1nah~~]])' scene=''>	+	<StructureSection load='2cnb' size='350' side='right' caption='Structure of UDP-galactose 4 epimerase complex with NAD and UDP-galactose (PDB entry [[2cnb]])' scene=''>
	== Function ==		== Function ==
	'''UDP-galactose 4-epimerase''' (GalE) reversibly converts UDP-galactose to UDP-glucose. GalE catalyzes the last step in galactose metabolism<ref>PMID:12534808</ref>. GalE uses NAD+ as a cofactor.		'''UDP-galactose 4-epimerase''' (GalE) reversibly converts UDP-galactose to UDP-glucose. GalE catalyzes the last step in galactose metabolism<ref>PMID:12534808</ref>. GalE uses NAD+ as a cofactor.
Line 5:		Line 5:
	== Disease ==		== Disease ==
	Mutations in GalE are the cause of galactosemia which is autosomal recessive disorder<ref>PMID:16301867</ref>.		Mutations in GalE are the cause of galactosemia which is autosomal recessive disorder<ref>PMID:16301867</ref>.
		+
		+	== Structural highlights ==
		+	GalE active site contains the substrate UDP-galactose (UDP-gal) and the cofactor NAD. Tyrosine residue is the catalytic base which produces the ketose intermediate<ref>PMID:16946458</ref>.
	</StructureSection>		</StructureSection>