Jaime Prilusky: New page: ==Cryo-EM structure of the 90S pre-ribosome== == Structural hig...

Jaime Prilusky — Tue, 26 Mar 2019 10:58:49 GMT

New page: ==Cryo-EM structure of the 90S pre-ribosome== <SX load='5jpq' viewer='molstar' size='340' side='right' caption='5jpq, resolution 7.30Å' scene=''> == Structural hig...

New page

==Cryo-EM structure of the 90S pre-ribosome==
<SX load='5jpq' viewer='molstar' size='340' side='right' caption='[[5jpq]], [[Resolution|resolution]] 7.30Å' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5jpq]] is a 55 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast], [http://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum] and [http://en.wikipedia.org/wiki/Chaetomium_thermophilum_ Chaetomium thermophilum ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JPQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JPQ FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jpq OCA], [http://pdbe.org/5jpq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jpq RCSB], [http://www.ebi.ac.uk/pdbsum/5jpq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jpq ProSAT]</span></td></tr>
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== Function ==
[[http://www.uniprot.org/uniprot/G0S3V7_CHATD G0S3V7_CHATD]] Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.[PIRNR:PIRNR006515] [[http://www.uniprot.org/uniprot/UTP21_YEAST UTP21_YEAST]] Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.<ref>PMID:15590835</ref>
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== Publication Abstract from PubMed ==
The 90S pre-ribosome is an early biogenesis intermediate formed during co-transcriptional ribosome formation, composed of approximately 70 assembly factors and several small nucleolar RNAs (snoRNAs) that associate with nascent pre-rRNA. We report the cryo-EM structure of the Chaetomium thermophilum 90S pre-ribosome, revealing how a network of biogenesis factors including 19 beta-propellers and large alpha-solenoid proteins engulfs the pre-rRNA. Within the 90S pre-ribosome, we identify the UTP-A, UTP-B, Mpp10-Imp3-Imp4, Bms1-Rcl1, and U3 snoRNP modules, which are organized around 5'-ETS and partially folded 18S rRNA. The U3 snoRNP is strategically positioned at the center of the 90S particle to perform its multiple tasks during pre-rRNA folding and processing. The architecture of the elusive 90S pre-ribosome gives unprecedented structural insight into the early steps of pre-rRNA maturation. Nascent rRNA that is co-transcriptionally folded and given a particular shape by encapsulation within a dedicated mold-like structure is reminiscent of how polypeptides use chaperone chambers for their protein folding.

Architecture of the 90S Pre-ribosome: A Structural View on the Birth of the Eukaryotic Ribosome.,Kornprobst M, Turk M, Kellner N, Cheng J, Flemming D, Kos-Braun I, Kos M, Thoms M, Berninghausen O, Beckmann R, Hurt E Cell. 2016 Jul 14;166(2):380-93. doi: 10.1016/j.cell.2016.06.014. PMID:27419870<ref>PMID:27419870</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5jpq" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ribosome 3D structures|Ribosome 3D structures]]
== References ==
<references/>
__TOC__
</SX>
[[Category: Baker's yeast]]
[[Category: Chaetomium thermophilum]]
[[Category: Beckmann, R]]
[[Category: Berninghausen, O]]
[[Category: Cheng, J]]
[[Category: Flemming, D]]
[[Category: Hurt, E]]
[[Category: Kornprobst, M]]
[[Category: Kos, M]]
[[Category: Kos-Braun, I C]]
[[Category: Thoms, M]]
[[Category: Turk, M]]
[[Category: Nuclear rnp]]
[[Category: Ribosome]]

User:Jaime Prilusky/Test/5jpq - Revision history

Jaime Prilusky: New page: ==Cryo-EM structure of the 90S pre-ribosome== == Structural hig...