User:Justin Smith/Sandbox 1 - Revision history

Justin Smith at 19:06, 13 April 2021

Justin Smith — Tue, 13 Apr 2021 19:06:14 GMT

←Older revision		Revision as of 19:06, 13 April 2021
Line 29:		Line 29:

	==Tunnels==		==Tunnels==
-	DGAT consists of 3 tunnels, a cytosolic tunnel, an ER-luminal funnel, and a membrane-embedded lateral gate. The cytosolic tunnel is the site of acyl-CoA binding, with the CoA group pointing at the cytosolic face and its acyl chain pointing towards the endoplasmic reticulum lumen. DAG then enters via the lateral gate on the luminal side via the lateral gate where it can then access the active site. The resulting product can then be released to either side of the membrane.	+	DGAT consists of 3 tunnels, a cytosolic tunnel, an ER-luminal funnel, and a membrane-embedded lateral gate. The cytosolic tunnel is the site of acyl-CoA binding, with the CoA group pointing at the cytosolic face and its acyl chain pointing towards the endoplasmic reticulum lumen. DAG then enters via the lateral gate on the luminal side via the lateral gate where it can then access the active site. The resulting product can then be released to either side of the membrane. <ref name="Sui">PMID:32433611</ref>.

	== References ==		== References ==


-	<ref name="Sui">PMID:32433611</ref>.

-	Rodriguez, A. M. (2020). Zooming in on the architecture of DGAT1, a transmembrane lipid factory. Baylor College of Medicine.
-
-	Stone, S. J. Mammalian Diacylglycerol Acyltransferases (DGAT). AOCS Lipid Library.

	<references/>		<references/>
	==Student Contributers==		==Student Contributers==
	*Justin Smith		*Justin Smith

Justin Smith at 19:05, 13 April 2021

Justin Smith — Tue, 13 Apr 2021 19:05:14 GMT

←Older revision		Revision as of 19:05, 13 April 2021
Line 26:		Line 26:
	== Structural highlights ==		== Structural highlights ==

-	This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.	+	This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.<ref name="wang">PMID:32433610</ref>.
		+
		+	==Tunnels==
		+	DGAT consists of 3 tunnels, a cytosolic tunnel, an ER-luminal funnel, and a membrane-embedded lateral gate. The cytosolic tunnel is the site of acyl-CoA binding, with the CoA group pointing at the cytosolic face and its acyl chain pointing towards the endoplasmic reticulum lumen. DAG then enters via the lateral gate on the luminal side via the lateral gate where it can then access the active site. The resulting product can then be released to either side of the membrane.
		+
	== References ==		== References ==
-	~~<ref name="wang">PMID:32433610</ref>.~~	+

	<ref name="Sui">PMID:32433611</ref>.		<ref name="Sui">PMID:32433611</ref>.

Justin Smith at 20:10, 16 March 2021

Justin Smith — Tue, 16 Mar 2021 20:10:38 GMT

←Older revision		Revision as of 20:10, 16 March 2021
Line 28:		Line 28:
	This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.		This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
	== References ==		== References ==
-	Wang, L, Qian, H, Nian, Y, Han, Y, Ren, Z, Hanzhi, Z, Hu, L, Prasad, B.V., Laganowsky, A, Yan, N, Zhou, M. (2020). Structure and mechanism of human diacylglycerol O-acyltransferase 1. Nature, 581, 329-332.
	<ref name="wang">PMID:32433610</ref>.		<ref name="wang">PMID:32433610</ref>.

-	Sui~~, X, Wang, K, Gluchowski, N, Elliott, S, Liao, M, Walther, T, Farese Jr, R. (2020). Structure and catalytic mechanism of a human triacylglycerol-synthesis enzyme. Nature, 581, 323-328~~.	+	<ref name="Sui">PMID:32433611</ref>.

	Rodriguez, A. M. (2020). Zooming in on the architecture of DGAT1, a transmembrane lipid factory. Baylor College of Medicine.		Rodriguez, A. M. (2020). Zooming in on the architecture of DGAT1, a transmembrane lipid factory. Baylor College of Medicine.

Justin Smith at 20:06, 16 March 2021

Justin Smith — Tue, 16 Mar 2021 20:06:23 GMT

←Older revision		Revision as of 20:06, 16 March 2021
Line 29:		Line 29:
	== References ==		== References ==
	Wang, L, Qian, H, Nian, Y, Han, Y, Ren, Z, Hanzhi, Z, Hu, L, Prasad, B.V., Laganowsky, A, Yan, N, Zhou, M. (2020). Structure and mechanism of human diacylglycerol O-acyltransferase 1. Nature, 581, 329-332.		Wang, L, Qian, H, Nian, Y, Han, Y, Ren, Z, Hanzhi, Z, Hu, L, Prasad, B.V., Laganowsky, A, Yan, N, Zhou, M. (2020). Structure and mechanism of human diacylglycerol O-acyltransferase 1. Nature, 581, 329-332.
		+	<ref name="wang">PMID:32433610</ref>.

	Sui, X, Wang, K, Gluchowski, N, Elliott, S, Liao, M, Walther, T, Farese Jr, R. (2020). Structure and catalytic mechanism of a human triacylglycerol-synthesis enzyme. Nature, 581, 323-328.		Sui, X, Wang, K, Gluchowski, N, Elliott, S, Liao, M, Walther, T, Farese Jr, R. (2020). Structure and catalytic mechanism of a human triacylglycerol-synthesis enzyme. Nature, 581, 323-328.

Justin Smith at 19:57, 16 March 2021

Justin Smith — Tue, 16 Mar 2021 19:57:33 GMT

←Older revision		Revision as of 19:57, 16 March 2021
Line 22:		Line 22:

	==Mecanism==		==Mecanism==
-	[[Image:Mec.PNG\|~~100~~ px\|left\|thumb\|Figure 1. Mecanism of base catalyized reaction]]	+	[[Image:Mec.PNG\|300 px\|left\|thumb\|Figure 1. Mecanism of base catalyized reaction]]

	== Structural highlights ==		== Structural highlights ==

Justin Smith at 19:55, 16 March 2021

Justin Smith — Tue, 16 Mar 2021 19:55:42 GMT

←Older revision		Revision as of 19:55, 16 March 2021
Line 20:		Line 20:
	<scene name='87/877628/His415/1'>His415 </scene>is the active base catalytic function		<scene name='87/877628/His415/1'>His415 </scene>is the active base catalytic function
	== Relevance ==		== Relevance ==
		+
		+	==Mecanism==
		+	[[Image:Mec.PNG\|100 px\|left\|thumb\|Figure 1. Mecanism of base catalyized reaction]]

	== Structural highlights ==		== Structural highlights ==

Justin Smith at 19:51, 16 March 2021

Justin Smith — Tue, 16 Mar 2021 19:51:50 GMT

←Older revision		Revision as of 19:51, 16 March 2021
Line 8:		Line 8:
	DGAT enzymes were identified more than 20 years ago		DGAT enzymes were identified more than 20 years ago

-	Polytopic Endoplasmic Reticulum Membrane Protein	+	Polytopic Endoplasmic Reticulum [https://en.wikipedia.org/wiki/Membrane_protein Membrane Protein]

	Embedded in membrane of ER		Embedded in membrane of ER

Justin Smith at 19:50, 16 March 2021

Justin Smith — Tue, 16 Mar 2021 19:50:03 GMT

←Older revision		Revision as of 19:50, 16 March 2021
Line 30:		Line 30:

	Rodriguez, A. M. (2020). Zooming in on the architecture of DGAT1, a transmembrane lipid factory. Baylor College of Medicine.		Rodriguez, A. M. (2020). Zooming in on the architecture of DGAT1, a transmembrane lipid factory. Baylor College of Medicine.
		+
	Stone, S. J. Mammalian Diacylglycerol Acyltransferases (DGAT). AOCS Lipid Library.		Stone, S. J. Mammalian Diacylglycerol Acyltransferases (DGAT). AOCS Lipid Library.

Justin Smith at 19:49, 16 March 2021

Justin Smith — Tue, 16 Mar 2021 19:49:14 GMT

←Older revision		Revision as of 19:49, 16 March 2021
Line 24:		Line 24:

	This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.		This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
-
-	</StructureSection>
	== References ==		== References ==
	Wang, L, Qian, H, Nian, Y, Han, Y, Ren, Z, Hanzhi, Z, Hu, L, Prasad, B.V., Laganowsky, A, Yan, N, Zhou, M. (2020). Structure and mechanism of human diacylglycerol O-acyltransferase 1. Nature, 581, 329-332.		Wang, L, Qian, H, Nian, Y, Han, Y, Ren, Z, Hanzhi, Z, Hu, L, Prasad, B.V., Laganowsky, A, Yan, N, Zhou, M. (2020). Structure and mechanism of human diacylglycerol O-acyltransferase 1. Nature, 581, 329-332.

Justin Smith at 19:48, 16 March 2021

Justin Smith — Tue, 16 Mar 2021 19:48:36 GMT

←Older revision		Revision as of 19:48, 16 March 2021
Line 18:		Line 18:

	==Active Site==		==Active Site==
-	<scene name='87/877628/His415/1'>His415</scene>is the active base catalytic function	+	<scene name='87/877628/His415/1'>His415 </scene>is the active base catalytic function
	== Relevance ==		== Relevance ==

Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	== References ==		== References ==
		+	Wang, L, Qian, H, Nian, Y, Han, Y, Ren, Z, Hanzhi, Z, Hu, L, Prasad, B.V., Laganowsky, A, Yan, N, Zhou, M. (2020). Structure and mechanism of human diacylglycerol O-acyltransferase 1. Nature, 581, 329-332.
		+
		+	Sui, X, Wang, K, Gluchowski, N, Elliott, S, Liao, M, Walther, T, Farese Jr, R. (2020). Structure and catalytic mechanism of a human triacylglycerol-synthesis enzyme. Nature, 581, 323-328.
		+
		+	Rodriguez, A. M. (2020). Zooming in on the architecture of DGAT1, a transmembrane lipid factory. Baylor College of Medicine.
		+	Stone, S. J. Mammalian Diacylglycerol Acyltransferases (DGAT). AOCS Lipid Library.
		+
	<references/>		<references/>
	==Student Contributers==		==Student Contributers==
	*Justin Smith		*Justin Smith