User:Rick H. Cote/PDE6 - Revision history

Rick H. Cote at 17:52, 6 July 2019

Rick H. Cote — Sat, 06 Jul 2019 17:52:19 GMT

←Older revision		Revision as of 17:52, 6 July 2019
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	== Structure of PDE6 holoenzyme ==		== Structure of PDE6 holoenzyme ==
	PDE6 is a member of the Class I cyclic nucleotide phosphodiesterases, of which 11 families exist in mammals. Rod photoreceptor PDE6 holoenzyme consists of a catalytic heterodimer (PDE6A and PDE6B) to which two identical inhibitory gamma subunits (PDE6G) bind, forming a tetrameric complex. Cone PDE6 holoenzyme is a catalytic homodimer (2 identical PDE6C subunits) to which cone-specific gamma-subunits (PDE6H) bind.		PDE6 is a member of the Class I cyclic nucleotide phosphodiesterases, of which 11 families exist in mammals. Rod photoreceptor PDE6 holoenzyme consists of a catalytic heterodimer (PDE6A and PDE6B) to which two identical inhibitory gamma subunits (PDE6G) bind, forming a tetrameric complex. Cone PDE6 holoenzyme is a catalytic homodimer (2 identical PDE6C subunits) to which cone-specific gamma-subunits (PDE6H) bind.
-	*6MZB -- cryo-EM structure of rod PDE6 holoenzyme at 3.4 A resolution.	+	*6MZB -- cryo-EM structure of rod PDE6 holoenzyme at 3.4 A resolution. <Structure load='6mZB' size='350' frame='true' align='right' caption='Rod PDE6 holoenzyme, from Gulati et al., 2019' scene='Insert optional scene name here' />

	== PDE6 catalytic subunit partial structures ==		== PDE6 catalytic subunit partial structures ==
-	*3dba -- Crystal structure of the cGMP-bound GAF a domain from the photoreceptor phosphodiesterase 6C <Structure load='3dba' size='~~500~~' frame='true' align='right' caption='GAFa domain of PDE6C with bound cGMP' scene='Insert optional scene name here' />	+	*3dba -- Crystal structure of the cGMP-bound GAF a domain from the photoreceptor phosphodiesterase 6C <Structure load='3dba' size='200' frame='true' align='right' caption='GAFa domain of PDE6C with bound cGMP' scene='Insert optional scene name here' />
	== PDE5/PDE6 chimeric structures ==		== PDE5/PDE6 chimeric structures ==
	*3jwq -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with sildenafil		*3jwq -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with sildenafil

Rick H. Cote at 17:48, 6 July 2019

Rick H. Cote — Sat, 06 Jul 2019 17:48:26 GMT

←Older revision		Revision as of 17:48, 6 July 2019
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	== Structure of PDE6 holoenzyme ==		== Structure of PDE6 holoenzyme ==
	PDE6 is a member of the Class I cyclic nucleotide phosphodiesterases, of which 11 families exist in mammals. Rod photoreceptor PDE6 holoenzyme consists of a catalytic heterodimer (PDE6A and PDE6B) to which two identical inhibitory gamma subunits (PDE6G) bind, forming a tetrameric complex. Cone PDE6 holoenzyme is a catalytic homodimer (2 identical PDE6C subunits) to which cone-specific gamma-subunits (PDE6H) bind.		PDE6 is a member of the Class I cyclic nucleotide phosphodiesterases, of which 11 families exist in mammals. Rod photoreceptor PDE6 holoenzyme consists of a catalytic heterodimer (PDE6A and PDE6B) to which two identical inhibitory gamma subunits (PDE6G) bind, forming a tetrameric complex. Cone PDE6 holoenzyme is a catalytic homodimer (2 identical PDE6C subunits) to which cone-specific gamma-subunits (PDE6H) bind.
-	*6MZB -- cryo-EM structure of rod PDE6 holoenzyme at 3.4 A resolution	+	*6MZB -- cryo-EM structure of rod PDE6 holoenzyme at 3.4 A resolution.

	== PDE6 catalytic subunit partial structures ==		== PDE6 catalytic subunit partial structures ==

Rick H. Cote at 17:44, 6 July 2019

Rick H. Cote — Sat, 06 Jul 2019 17:44:15 GMT

←Older revision		Revision as of 17:44, 6 July 2019
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	== Structure of PDE6 holoenzyme ==		== Structure of PDE6 holoenzyme ==
	PDE6 is a member of the Class I cyclic nucleotide phosphodiesterases, of which 11 families exist in mammals. Rod photoreceptor PDE6 holoenzyme consists of a catalytic heterodimer (PDE6A and PDE6B) to which two identical inhibitory gamma subunits (PDE6G) bind, forming a tetrameric complex. Cone PDE6 holoenzyme is a catalytic homodimer (2 identical PDE6C subunits) to which cone-specific gamma-subunits (PDE6H) bind.		PDE6 is a member of the Class I cyclic nucleotide phosphodiesterases, of which 11 families exist in mammals. Rod photoreceptor PDE6 holoenzyme consists of a catalytic heterodimer (PDE6A and PDE6B) to which two identical inhibitory gamma subunits (PDE6G) bind, forming a tetrameric complex. Cone PDE6 holoenzyme is a catalytic homodimer (2 identical PDE6C subunits) to which cone-specific gamma-subunits (PDE6H) bind.
		+	*6MZB -- cryo-EM structure of rod PDE6 holoenzyme at 3.4 A resolution

	== PDE6 catalytic subunit partial structures ==		== PDE6 catalytic subunit partial structures ==

Rick H. Cote at 13:48, 14 June 2011

Rick H. Cote — Tue, 14 Jun 2011 13:48:37 GMT

←Older revision		Revision as of 13:48, 14 June 2011
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	*3dba -- Crystal structure of the cGMP-bound GAF a domain from the photoreceptor phosphodiesterase 6C <Structure load='3dba' size='500' frame='true' align='right' caption='GAFa domain of PDE6C with bound cGMP' scene='Insert optional scene name here' />		*3dba -- Crystal structure of the cGMP-bound GAF a domain from the photoreceptor phosphodiesterase 6C <Structure load='3dba' size='500' frame='true' align='right' caption='GAFa domain of PDE6C with bound cGMP' scene='Insert optional scene name here' />
	== PDE5/PDE6 chimeric structures ==		== PDE5/PDE6 chimeric structures ==
-	*3jwq -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with sildenafil ~~<Structure load='3jwq' size='500' frame='true' align='right' caption='PDE5/6 chimeric catalytic domain with bound sildenafil' scene='Insert optional scene name here' />~~	+	*3jwq -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with sildenafil
	*3jwr -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with 3-isobutyl-1-methylxanthine (IBMX) and PDE6 gamma-subunit inhibitory peptide 70-87		*3jwr -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with 3-isobutyl-1-methylxanthine (IBMX) and PDE6 gamma-subunit inhibitory peptide 70-87

Rick H. Cote at 13:46, 14 June 2011

Rick H. Cote — Tue, 14 Jun 2011 13:46:27 GMT

←Older revision		Revision as of 13:46, 14 June 2011
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	*3dba -- Crystal structure of the cGMP-bound GAF a domain from the photoreceptor phosphodiesterase 6C <Structure load='3dba' size='500' frame='true' align='right' caption='GAFa domain of PDE6C with bound cGMP' scene='Insert optional scene name here' />		*3dba -- Crystal structure of the cGMP-bound GAF a domain from the photoreceptor phosphodiesterase 6C <Structure load='3dba' size='500' frame='true' align='right' caption='GAFa domain of PDE6C with bound cGMP' scene='Insert optional scene name here' />
	== PDE5/PDE6 chimeric structures ==		== PDE5/PDE6 chimeric structures ==
-	*3jwq -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with sildenafil	+	*3jwq -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with sildenafil <Structure load='3jwq' size='500' frame='true' align='right' caption='PDE5/6 chimeric catalytic domain with bound sildenafil' scene='Insert optional scene name here' />
	*3jwr -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with 3-isobutyl-1-methylxanthine (IBMX) and PDE6 gamma-subunit inhibitory peptide 70-87		*3jwr -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with 3-isobutyl-1-methylxanthine (IBMX) and PDE6 gamma-subunit inhibitory peptide 70-87

Rick H. Cote at 13:44, 14 June 2011

Rick H. Cote — Tue, 14 Jun 2011 13:44:35 GMT

←Older revision		Revision as of 13:44, 14 June 2011
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	== PDE6 catalytic subunit partial structures ==		== PDE6 catalytic subunit partial structures ==
-	*3dba -- Crystal structure of the cGMP-bound GAF a domain from the photoreceptor phosphodiesterase 6C <Structure load='3dba' size='500' frame='true' align='right' caption='~~Insert caption here~~' scene='Insert optional scene name here' />	+	*3dba -- Crystal structure of the cGMP-bound GAF a domain from the photoreceptor phosphodiesterase 6C <Structure load='3dba' size='500' frame='true' align='right' caption='GAFa domain of PDE6C with bound cGMP' scene='Insert optional scene name here' />
	== PDE5/PDE6 chimeric structures ==		== PDE5/PDE6 chimeric structures ==
	*3jwq -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with sildenafil		*3jwq -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with sildenafil

Rick H. Cote at 13:42, 14 June 2011

Rick H. Cote — Tue, 14 Jun 2011 13:42:26 GMT

←Older revision		Revision as of 13:42, 14 June 2011
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	== PDE6 catalytic subunit partial structures ==		== PDE6 catalytic subunit partial structures ==
-	*3dba -- Crystal structure of the cGMP-bound GAF a domain from the photoreceptor phosphodiesterase 6C	+	*3dba -- Crystal structure of the cGMP-bound GAF a domain from the photoreceptor phosphodiesterase 6C <Structure load='3dba' size='500' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' />
	== PDE5/PDE6 chimeric structures ==		== PDE5/PDE6 chimeric structures ==
	*3jwq -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with sildenafil		*3jwq -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with sildenafil

Rick H. Cote: /* PDE5/PDE6 structures */

Rick H. Cote — Tue, 14 Jun 2011 03:38:40 GMT

PDE5/PDE6 structures

←Older revision		Revision as of 03:38, 14 June 2011
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	== PDE6 catalytic subunit partial structures ==		== PDE6 catalytic subunit partial structures ==
	*3dba -- Crystal structure of the cGMP-bound GAF a domain from the photoreceptor phosphodiesterase 6C		*3dba -- Crystal structure of the cGMP-bound GAF a domain from the photoreceptor phosphodiesterase 6C
-	== PDE5/PDE6 structures ==	+	== PDE5/PDE6 chimeric structures ==
	*3jwq -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with sildenafil		*3jwq -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with sildenafil
	*3jwr -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with 3-isobutyl-1-methylxanthine (IBMX) and PDE6 gamma-subunit inhibitory peptide 70-87		*3jwr -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with 3-isobutyl-1-methylxanthine (IBMX) and PDE6 gamma-subunit inhibitory peptide 70-87
		+
	== PDE6 inhibitory γ-subunit structures ==		== PDE6 inhibitory γ-subunit structures ==
	*2ju4 -- NMR structure of the gamma subunit of cGMP phosphodiesterase		*2ju4 -- NMR structure of the gamma subunit of cGMP phosphodiesterase
	*1fqj -- CRYSTAL STRUCTURE OF THE HETEROTRIMERIC COMPLEX OF THE RGS DOMAIN OF RGS9, THE GAMMA SUBUNIT OF PHOSPHODIESTERASE AND THE GT/I1 CHIMERA ALPHA SUBUNIT [(RGS9)-(PDEGAMMA)-(GT/I1ALPHA)-(GDP)-(ALF4-)-(MG2+)]		*1fqj -- CRYSTAL STRUCTURE OF THE HETEROTRIMERIC COMPLEX OF THE RGS DOMAIN OF RGS9, THE GAMMA SUBUNIT OF PHOSPHODIESTERASE AND THE GT/I1 CHIMERA ALPHA SUBUNIT [(RGS9)-(PDEGAMMA)-(GT/I1ALPHA)-(GDP)-(ALF4-)-(MG2+)]

Rick H. Cote: /* PDE6 inhibitory gamma subunit structures */

Rick H. Cote — Tue, 14 Jun 2011 03:38:13 GMT

PDE6 inhibitory gamma subunit structures

←Older revision		Revision as of 03:38, 14 June 2011
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	*3jwq -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with sildenafil		*3jwq -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with sildenafil
	*3jwr -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with 3-isobutyl-1-methylxanthine (IBMX) and PDE6 gamma-subunit inhibitory peptide 70-87		*3jwr -- Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with 3-isobutyl-1-methylxanthine (IBMX) and PDE6 gamma-subunit inhibitory peptide 70-87
-	== PDE6 inhibitory ~~gamma~~ subunit structures ==	+	== PDE6 inhibitory γ-subunit structures ==
	*2ju4 -- NMR structure of the gamma subunit of cGMP phosphodiesterase		*2ju4 -- NMR structure of the gamma subunit of cGMP phosphodiesterase
	*1fqj -- CRYSTAL STRUCTURE OF THE HETEROTRIMERIC COMPLEX OF THE RGS DOMAIN OF RGS9, THE GAMMA SUBUNIT OF PHOSPHODIESTERASE AND THE GT/I1 CHIMERA ALPHA SUBUNIT [(RGS9)-(PDEGAMMA)-(GT/I1ALPHA)-(GDP)-(ALF4-)-(MG2+)]		*1fqj -- CRYSTAL STRUCTURE OF THE HETEROTRIMERIC COMPLEX OF THE RGS DOMAIN OF RGS9, THE GAMMA SUBUNIT OF PHOSPHODIESTERASE AND THE GT/I1 CHIMERA ALPHA SUBUNIT [(RGS9)-(PDEGAMMA)-(GT/I1ALPHA)-(GDP)-(ALF4-)-(MG2+)]

Rick H. Cote: User:Rick H. Cote/sandbox moved to User:Rick H. Cote/PDE6

Rick H. Cote — Tue, 14 Jun 2011 03:35:36 GMT

User:Rick H. Cote/sandbox moved to User:Rick H. Cote/PDE6

←Older revision

Revision as of 03:35, 14 June 2011