V-ATPase - Revision history

Michal Harel at 10:55, 28 February 2016

Michal Harel — Sun, 28 Feb 2016 10:55:14 GMT

←Older revision		Revision as of 10:55, 28 February 2016
Line 16:		Line 16:
	==Mechanism of rotation==		==Mechanism of rotation==

-	==V-ATPase structures==	+	==3D V-ATPase structures==

-	~~===PDB===~~	+	See [[ATPase]]
-		+
-	~~====V1 complex====~~	+
-	[[~~3j0j]]: Fitted structure of Thermus thermophilus in a 9.7Å resolution cryo-EM map.~~	+
-		+
-	~~[[3a5c]]~~	+
-		+
-	~~[[3a5d]]~~	+
-	~~====A3B3 complex====~~	+
-	~~[[3gqb]]~~	+
-		+
-	~~====Subunit C====~~	+
-	~~[[1r5z]]~~	+
-		+
-	~~[[1u7l]]~~	+
-		+
-	~~[[1v9m]]~~	+
-		+
-	~~====Subunit E====~~	+
-	~~[[2kz9]]~~	+
-		+
-	~~[[3k5b]]~~	+
-		+
-	~~[[3v6i]]~~	+
-		+
-	~~====Subunit F====~~	+
-	~~[[2d00]]~~	+
-		+
-	~~====Subunit G====~~	+
-	~~[[2kwy]]~~	+
-		+
-	~~[[2k88]]~~	+
-		+
-	~~====Subunit H====~~	+
-	~~[[1ho8]]~~	+
-		+
-	~~====Vo complex====~~	+
-		+
-	~~[[3aou]]~~	+
-		+
-	~~[[2db4]]~~	+
-		+
-	~~[[2bl2]]~~	+
-		+
-	~~[[2cyd]]~~	+
-		+
-	~~====Subunit a====~~	+
-	~~[[2rpw]]~~	+
-		+
-	~~[[2nvj~~]]	+

	===EMDB===		===EMDB===

Gydo van Zundert at 14:15, 6 September 2012

Gydo van Zundert — Thu, 06 Sep 2012 14:15:36 GMT

←Older revision		Revision as of 14:15, 6 September 2012
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	====V1 complex====		====V1 complex====
-	[[3j0j]]: Fitted structure of Thermus ~~Thermophilus~~ in a 9.7Å resolution cryo-EM map.	+	[[3j0j]]: Fitted structure of Thermus thermophilus in a 9.7Å resolution cryo-EM map.

	[[3a5c]]		[[3a5c]]

Gydo van Zundert at 14:13, 6 September 2012

Gydo van Zundert — Thu, 06 Sep 2012 14:13:38 GMT

←Older revision		Revision as of 14:13, 6 September 2012
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	===V1-domain===		===V1-domain===
	<StructureSection load='3a5c' size='250' side='right' caption='V1-domain of V-ATPase of Thermus Thermophilus (PDB entry [[3j0j]])' scene='V-ATPase/V1-part/1'>		<StructureSection load='3a5c' size='250' side='right' caption='V1-domain of V-ATPase of Thermus Thermophilus (PDB entry [[3j0j]])' scene='V-ATPase/V1-part/1'>
-	The <scene name='V-ATPase/V1-part/1'>V1-domain</scene> consist of the A3B3 complex where the hydrolysis of ATP occurs (<scene name='V-ATPase/V1-part/3'>A is blue, B is red</scene>), subunit C (<scene name='V-ATPase/V1-part/5'>green</scene>), the D-subunit which is the central stalk (<scene name='V-ATPase/V1-part/2'>purple</scene>), subunit E (<scene name='V-ATPase/V1-part/6'>orange</scene>), subunit F (<scene name='V-ATPase/V1-part/4'>yellow</scene>)and subunit G (<scene name='V-ATPase/V1-part/7'>dark green</scene>).	+	The <scene name='V-ATPase/V1-part/1'>V1-domain</scene> consist of the A3B3 complex where the hydrolysis of ATP occurs (<scene name='V-ATPase/V1-part/3'>A is blue, B is red</scene>), subunit C (<scene name='V-ATPase/V1-part/8'>green</scene>), the D-subunit which is the central stalk (<scene name='V-ATPase/V1-part/2'>purple</scene>), subunit E (<scene name='V-ATPase/V1-part/6'>orange</scene>), subunit F (<scene name='V-ATPase/V1-part/4'>yellow</scene>)and subunit G (<scene name='V-ATPase/V1-part/7'>dark green</scene>).
	</StructureSection>		</StructureSection>

Gydo van Zundert at 14:01, 6 September 2012

Gydo van Zundert — Thu, 06 Sep 2012 14:01:11 GMT

←Older revision		Revision as of 14:01, 6 September 2012
Line 10:		Line 10:
	<StructureSection load='3a5c' size='250' side='right' caption='V1-domain of V-ATPase of Thermus Thermophilus (PDB entry [[3j0j]])' scene='V-ATPase/V1-part/1'>		<StructureSection load='3a5c' size='250' side='right' caption='V1-domain of V-ATPase of Thermus Thermophilus (PDB entry [[3j0j]])' scene='V-ATPase/V1-part/1'>
	The <scene name='V-ATPase/V1-part/1'>V1-domain</scene> consist of the A3B3 complex where the hydrolysis of ATP occurs (<scene name='V-ATPase/V1-part/3'>A is blue, B is red</scene>), subunit C (<scene name='V-ATPase/V1-part/5'>green</scene>), the D-subunit which is the central stalk (<scene name='V-ATPase/V1-part/2'>purple</scene>), subunit E (<scene name='V-ATPase/V1-part/6'>orange</scene>), subunit F (<scene name='V-ATPase/V1-part/4'>yellow</scene>)and subunit G (<scene name='V-ATPase/V1-part/7'>dark green</scene>).		The <scene name='V-ATPase/V1-part/1'>V1-domain</scene> consist of the A3B3 complex where the hydrolysis of ATP occurs (<scene name='V-ATPase/V1-part/3'>A is blue, B is red</scene>), subunit C (<scene name='V-ATPase/V1-part/5'>green</scene>), the D-subunit which is the central stalk (<scene name='V-ATPase/V1-part/2'>purple</scene>), subunit E (<scene name='V-ATPase/V1-part/6'>orange</scene>), subunit F (<scene name='V-ATPase/V1-part/4'>yellow</scene>)and subunit G (<scene name='V-ATPase/V1-part/7'>dark green</scene>).
-
	</StructureSection>		</StructureSection>
		+
		+	===V0-domain===

	==Mechanism of rotation==		==Mechanism of rotation==

Gydo van Zundert at 13:53, 6 September 2012

Gydo van Zundert — Thu, 06 Sep 2012 13:53:51 GMT

←Older revision		Revision as of 13:53, 6 September 2012
Line 1:		Line 1:
	==Introduction==		==Introduction==
-	Vacuolar (H+)-ATPases (V-ATPases)<ref>PMID:17912264</ref> are mainly found in vacuoles of eukaryotic cells where they catalyze the hydrolysis of [[ATP]] in order to transport solutes.	+	Vacuolar (H+)-ATPases (V-ATPases)<ref>PMID:17912264</ref> are mainly found in vacuoles of eukaryotic cells where they catalyze the hydrolysis of [[ATP]] in order to transport solutes. V-ATPases are structurally and mechanically related to F- and A-ATPases.<ref>PMID:15473999</ref>

	==V-ATPase components==		==V-ATPase components==
-	The structure of the whole V-ATPase complex can be divided in two domains. The V1 domain, which consist of eight different sub-units (A-H) and is responsible for the hydrolysis of ATP, and the intermembrane V0 domain consisting of six different sub-units and which ~~transports~~ the ~~protons~~.<ref>PMID:20450191</ref>	+	The structure of the whole V-ATPase complex can be divided in two domains. The V1 domain, which consist of eight different sub-units (A-H) and is responsible for the hydrolysis of ATP, and the intermembrane V0 domain consisting of six different sub-units (a, d, e, c, c' and c") and which acts as a proton translocator from the cytoplasm to the lumen.<ref>PMID:20450191</ref>
		+
		+	ATP hydrolysis occurs at catalytic sites located at the interface of the A and B subunits.
		+
		+	===V1-domain===
		+	<StructureSection load='3a5c' size='250' side='right' caption='V1-domain of V-ATPase of Thermus Thermophilus (PDB entry [[3j0j]])' scene='V-ATPase/V1-part/1'>
		+	The <scene name='V-ATPase/V1-part/1'>V1-domain</scene> consist of the A3B3 complex where the hydrolysis of ATP occurs (<scene name='V-ATPase/V1-part/3'>A is blue, B is red</scene>), subunit C (<scene name='V-ATPase/V1-part/5'>green</scene>), the D-subunit which is the central stalk (<scene name='V-ATPase/V1-part/2'>purple</scene>), subunit E (<scene name='V-ATPase/V1-part/6'>orange</scene>), subunit F (<scene name='V-ATPase/V1-part/4'>yellow</scene>)and subunit G (<scene name='V-ATPase/V1-part/7'>dark green</scene>).
		+
		+	</StructureSection>

	==Mechanism of rotation==		==Mechanism of rotation==

Gydo van Zundert at 12:20, 6 September 2012

Gydo van Zundert — Thu, 06 Sep 2012 12:20:52 GMT

←Older revision		Revision as of 12:20, 6 September 2012
Line 59:		Line 59:

	[[2nvj]]		[[2nvj]]
-
-
-

	===EMDB===		===EMDB===

Gydo van Zundert at 12:20, 6 September 2012

Gydo van Zundert — Thu, 06 Sep 2012 12:20:32 GMT

←Older revision		Revision as of 12:20, 6 September 2012
Line 1:		Line 1:
-	== Your Heading Here (maybe something like 'Structure') ==
-	<StructureSection load='3j0j' size='350' side='right' caption='Fitted structure of Thermus thermophilus V-ATPase, based on the EMD-5335 (PDB entry [[3j0j]])' scene=''>
-	Anything in this section will appear adjacent to the 3D structure and will be scrollable.
-
-	<scene name='V-ATPase/Adp/1'>TextToBeDisplayed</scene>
-	</StructureSection>
-
	==Introduction==		==Introduction==
-	V-~~ATPase~~ are mainly found in vacuoles of eukaryotic cells where they catalyze the ~~hydrolyzation~~ of [[ATP]] in order to transport solutes.	+	Vacuolar (H+)-ATPases (V-ATPases)<ref>PMID:17912264</ref> are mainly found in vacuoles of eukaryotic cells where they catalyze the hydrolysis of [[ATP]] in order to transport solutes.

	==V-ATPase components==		==V-ATPase components==
-	The structure of the whole V-ATPase complex can be divided in two domains. The V1 domain, which consist of eight different sub-units (A-H) and is responsible for the ~~hydrolyzation~~ of ATP, and the intermembrane V0 domain consisting of six different sub-units ~~(a-f)~~ and which transports the protons.	+	The structure of the whole V-ATPase complex can be divided in two domains. The V1 domain, which consist of eight different sub-units (A-H) and is responsible for the hydrolysis of ATP, and the intermembrane V0 domain consisting of six different sub-units and which transports the protons.<ref>PMID:20450191</ref>
		+
	==Mechanism of rotation==		==Mechanism of rotation==

Line 16:		Line 10:

	===PDB===		===PDB===
-	[[3j0j]]	+
		+	====V1 complex====
		+	[[3j0j]]: Fitted structure of Thermus Thermophilus in a 9.7Å resolution cryo-EM map.

	[[3a5c]]		[[3a5c]]

-	[[~~3k5b~~]]	+	[[3a5d]]
		+	====A3B3 complex====
		+	[[3gqb]]

		+	====Subunit C====
	[[1r5z]]		[[1r5z]]
		+
		+	[[1u7l]]
		+
		+	[[1v9m]]
		+
		+	====Subunit E====
		+	[[2kz9]]
		+
		+	[[3k5b]]
		+
		+	[[3v6i]]
		+
		+	====Subunit F====
		+	[[2d00]]
		+
		+	====Subunit G====
		+	[[2kwy]]
		+
		+	[[2k88]]
		+
		+	====Subunit H====
		+	[[1ho8]]
		+
		+	====Vo complex====
		+
		+	[[3aou]]
		+
		+	[[2db4]]
		+
		+	[[2bl2]]
		+
		+	[[2cyd]]
		+
		+	====Subunit a====
		+	[[2rpw]]
		+
		+	[[2nvj]]
		+
		+


	===EMDB===		===EMDB===
-	5335	+	[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/5335_summary.html 5335]: 9.7Å resolution map of Thermus Thermophilus V-ATPase.
		+
		+	[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1888_summary.html 1888]: 16Å resolution map of Thermus Thermophilus V-ATPase.
		+
		+	[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1640_summary.html 1640]: 25Å resolution map of Saccharomyces cerevisiae V-ATPase.
		+
		+	[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1590_summary.html 1590]: 17Å resolution map of Manduca sexta V-ATPase.
		+
	==References==		==References==
		+
		+	<references />

Gydo van Zundert: V-ATPase start page.

Gydo van Zundert — Thu, 06 Sep 2012 09:29:15 GMT

V-ATPase start page.

←Older revision		Revision as of 09:29, 6 September 2012
Line 1:		Line 1:
-	~~#REDIRECT~~ [[ATP ~~synthase~~]]	+	== Your Heading Here (maybe something like 'Structure') ==
		+	<StructureSection load='3j0j' size='350' side='right' caption='Fitted structure of Thermus thermophilus V-ATPase, based on the EMD-5335 (PDB entry [[3j0j]])' scene=''>
		+	Anything in this section will appear adjacent to the 3D structure and will be scrollable.
		+
		+	<scene name='V-ATPase/Adp/1'>TextToBeDisplayed</scene>
		+	</StructureSection>
		+
		+	==Introduction==
		+	V-ATPase are mainly found in vacuoles of eukaryotic cells where they catalyze the hydrolyzation of [[ATP]] in order to transport solutes.
		+
		+	==V-ATPase components==
		+	The structure of the whole V-ATPase complex can be divided in two domains. The V1 domain, which consist of eight different sub-units (A-H) and is responsible for the hydrolyzation of ATP, and the intermembrane V0 domain consisting of six different sub-units (a-f) and which transports the protons.
		+	==Mechanism of rotation==
		+
		+	==V-ATPase structures==
		+
		+	===PDB===
		+	[[3j0j]]
		+
		+	[[3a5c]]
		+
		+	[[3k5b]]
		+
		+	[[1r5z]]
		+
		+
		+	===EMDB===
		+	5335
		+	==References==

Alexander Berchansky: Redirecting to ATP synthase

Alexander Berchansky — Wed, 23 Feb 2011 07:35:11 GMT

Redirecting to ATP synthase

New page

#REDIRECT [[ATP synthase]]