Wherland Sandbox 3 - Revision history

Scot Wherland at 14:01, 19 July 2015

Scot Wherland — Sun, 19 Jul 2015 14:01:29 GMT

←Older revision		Revision as of 14:01, 19 July 2015
Line 9:		Line 9:
	<scene name='70/707393/3fpx_domains_cu_2ndry/1'>structure</scene>. Adding the		<scene name='70/707393/3fpx_domains_cu_2ndry/1'>structure</scene>. Adding the
	<scene name='70/707393/3fpx_domains_cu_ligands/4'>ligands</scene> to the Cu ions shows that they come from different domains. The ligands of the T1 Cu ion (His-395 and 458 and Cys-453) all come from the yellow domain 3. The ligands of one T3 Cu ion come from the yellow domain 3 (His-400 and 452) and the blue domain 1(His 111). The ligands of the other T3 Cu ion also come from the yellow and blue domains 3 and 1, but two (His-66 and 109) come from the blue domain 1 and only one from the yellow domain 3 (His-454). The ligands of the T2 Cu ion come from the blue domain 1 (His 64) and yellow domain 3 (His-398) and in addition there are three water molecules, one in the center of the TNC, one bridging the T3 Cu ions, and one just associated with the T2 Cu ion. None of the ligands come from the blue domain 2, the middle of the sequence. The ligands can be seen better without the surrounding chains, and with the bridging		<scene name='70/707393/3fpx_domains_cu_ligands/4'>ligands</scene> to the Cu ions shows that they come from different domains. The ligands of the T1 Cu ion (His-395 and 458 and Cys-453) all come from the yellow domain 3. The ligands of one T3 Cu ion come from the yellow domain 3 (His-400 and 452) and the blue domain 1(His 111). The ligands of the other T3 Cu ion also come from the yellow and blue domains 3 and 1, but two (His-66 and 109) come from the blue domain 1 and only one from the yellow domain 3 (His-454). The ligands of the T2 Cu ion come from the blue domain 1 (His 64) and yellow domain 3 (His-398) and in addition there are three water molecules, one in the center of the TNC, one bridging the T3 Cu ions, and one just associated with the T2 Cu ion. None of the ligands come from the blue domain 2, the middle of the sequence. The ligands can be seen better without the surrounding chains, and with the bridging
-	<scene name='70/707393/3fpx_domains_cu_ligands_links/2'>bridging</scene> residues between ligands (Trp-65, Ser-110, and Leu-399. The Cys-453 ligand of T1 connects directly to each of the T3 ions with a His ligand using the H452-C453-H454 segment. The two T3 ions are bridged by H398-L399-H400, T2 connects to one T3 by H64-W65-H66 and the other by H398-L399-H400. These bridges between Cu ions can serve as electron transfer (ET) pathways~~. The <scene name='70/707393/3fpx_cu_ligands_exposure/1'>exposure</scene> of the Cu centers is important to the control of their reactivity~~.	+	<scene name='70/707393/3fpx_domains_cu_ligands_links/2'>bridging</scene> residues between ligands (Trp-65, Ser-110, and Leu-399. The Cys-453 ligand of T1 connects directly to each of the T3 ions with a His ligand using the H452-C453-H454 segment. The two T3 ions are bridged by H398-L399-H400, T2 connects to one T3 by H64-W65-H66 and the other by H398-L399-H400. These bridges between Cu ions can serve as electron transfer (ET) pathways.
-	Many of the MCOs have sugars on their surface. In this case there are 4 <scene name='70/707393/3fpx_domains_cu_sugar/1'>sugar chains</scene>.	+	Many of the MCOs have sugars on their surface. In this case there are 4 <scene name='70/707393/3fpx_domains_cu_sugar/1'>sugar chains</scene>.
		+	The <scene name='70/707393/3fpx_cu_ligands_exposure/2'>exposure</scene> of the Cu centers is important to the control of their reactivity. Stop the spinning and manipulate the structure to see (click an atom to identify it) His-458 of the T1 copper and His-111 of one of the T3 Cu ions at the bottom of a deep pit. Peptide is gray, sugars are pale blue.
	== Disease ==		== Disease ==

Scot Wherland at 13:46, 19 July 2015

Scot Wherland — Sun, 19 Jul 2015 13:46:02 GMT

←Older revision		Revision as of 13:46, 19 July 2015
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	<scene name='70/707393/3fpx_domains_cu_2ndry/1'>structure</scene>. Adding the		<scene name='70/707393/3fpx_domains_cu_2ndry/1'>structure</scene>. Adding the
	<scene name='70/707393/3fpx_domains_cu_ligands/4'>ligands</scene> to the Cu ions shows that they come from different domains. The ligands of the T1 Cu ion (His-395 and 458 and Cys-453) all come from the yellow domain 3. The ligands of one T3 Cu ion come from the yellow domain 3 (His-400 and 452) and the blue domain 1(His 111). The ligands of the other T3 Cu ion also come from the yellow and blue domains 3 and 1, but two (His-66 and 109) come from the blue domain 1 and only one from the yellow domain 3 (His-454). The ligands of the T2 Cu ion come from the blue domain 1 (His 64) and yellow domain 3 (His-398) and in addition there are three water molecules, one in the center of the TNC, one bridging the T3 Cu ions, and one just associated with the T2 Cu ion. None of the ligands come from the blue domain 2, the middle of the sequence. The ligands can be seen better without the surrounding chains, and with the bridging		<scene name='70/707393/3fpx_domains_cu_ligands/4'>ligands</scene> to the Cu ions shows that they come from different domains. The ligands of the T1 Cu ion (His-395 and 458 and Cys-453) all come from the yellow domain 3. The ligands of one T3 Cu ion come from the yellow domain 3 (His-400 and 452) and the blue domain 1(His 111). The ligands of the other T3 Cu ion also come from the yellow and blue domains 3 and 1, but two (His-66 and 109) come from the blue domain 1 and only one from the yellow domain 3 (His-454). The ligands of the T2 Cu ion come from the blue domain 1 (His 64) and yellow domain 3 (His-398) and in addition there are three water molecules, one in the center of the TNC, one bridging the T3 Cu ions, and one just associated with the T2 Cu ion. None of the ligands come from the blue domain 2, the middle of the sequence. The ligands can be seen better without the surrounding chains, and with the bridging
-	<scene name='70/707393/3fpx_domains_cu_ligands_links/2'>bridging</scene> residues between ligands (Trp-65, Ser-110, and Leu-399. The Cys-453 ligand of T1 connects directly to each of the T3 ions with a His ligand using the H452-C453-H454 segment. The two T3 ions are bridged by H398-L399-H400, T2 connects to one T3 by H64-W65-H66 and the other by H398-L399-H400. These bridges between Cu ions can serve as electron transfer (ET) pathways.	+	<scene name='70/707393/3fpx_domains_cu_ligands_links/2'>bridging</scene> residues between ligands (Trp-65, Ser-110, and Leu-399. The Cys-453 ligand of T1 connects directly to each of the T3 ions with a His ligand using the H452-C453-H454 segment. The two T3 ions are bridged by H398-L399-H400, T2 connects to one T3 by H64-W65-H66 and the other by H398-L399-H400. These bridges between Cu ions can serve as electron transfer (ET) pathways. The <scene name='70/707393/3fpx_cu_ligands_exposure/1'>exposure</scene> of the Cu centers is important to the control of their reactivity.
	Many of the MCOs have sugars on their surface. In this case there are 4 <scene name='70/707393/3fpx_domains_cu_sugar/1'>sugar chains</scene>.		Many of the MCOs have sugars on their surface. In this case there are 4 <scene name='70/707393/3fpx_domains_cu_sugar/1'>sugar chains</scene>.
	== Disease ==		== Disease ==

Scot Wherland at 13:24, 19 July 2015

Scot Wherland — Sun, 19 Jul 2015 13:24:46 GMT

←Older revision		Revision as of 13:24, 19 July 2015
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	<scene name='70/707393/3fpx_domains_cu_2ndry/1'>structure</scene>. Adding the		<scene name='70/707393/3fpx_domains_cu_2ndry/1'>structure</scene>. Adding the
	<scene name='70/707393/3fpx_domains_cu_ligands/4'>ligands</scene> to the Cu ions shows that they come from different domains. The ligands of the T1 Cu ion (His-395 and 458 and Cys-453) all come from the yellow domain 3. The ligands of one T3 Cu ion come from the yellow domain 3 (His-400 and 452) and the blue domain 1(His 111). The ligands of the other T3 Cu ion also come from the yellow and blue domains 3 and 1, but two (His-66 and 109) come from the blue domain 1 and only one from the yellow domain 3 (His-454). The ligands of the T2 Cu ion come from the blue domain 1 (His 64) and yellow domain 3 (His-398) and in addition there are three water molecules, one in the center of the TNC, one bridging the T3 Cu ions, and one just associated with the T2 Cu ion. None of the ligands come from the blue domain 2, the middle of the sequence. The ligands can be seen better without the surrounding chains, and with the bridging		<scene name='70/707393/3fpx_domains_cu_ligands/4'>ligands</scene> to the Cu ions shows that they come from different domains. The ligands of the T1 Cu ion (His-395 and 458 and Cys-453) all come from the yellow domain 3. The ligands of one T3 Cu ion come from the yellow domain 3 (His-400 and 452) and the blue domain 1(His 111). The ligands of the other T3 Cu ion also come from the yellow and blue domains 3 and 1, but two (His-66 and 109) come from the blue domain 1 and only one from the yellow domain 3 (His-454). The ligands of the T2 Cu ion come from the blue domain 1 (His 64) and yellow domain 3 (His-398) and in addition there are three water molecules, one in the center of the TNC, one bridging the T3 Cu ions, and one just associated with the T2 Cu ion. None of the ligands come from the blue domain 2, the middle of the sequence. The ligands can be seen better without the surrounding chains, and with the bridging
-	<scene name='70/707393/3fpx_domains_cu_ligands_links/2'>bridging</scene> residues between ligands (Trp-65, Ser-110, and Leu-399. The Cys-453 ligand of T1 connects directly to each of the T3 ions with a His ligand using the H452-C453-H454 segment. The two T3 ions are bridged by H398-L399-H400, T2 connects to one T3 by H64-W65-H66 and the other by H398-L399-H400.	+	<scene name='70/707393/3fpx_domains_cu_ligands_links/2'>bridging</scene> residues between ligands (Trp-65, Ser-110, and Leu-399. The Cys-453 ligand of T1 connects directly to each of the T3 ions with a His ligand using the H452-C453-H454 segment. The two T3 ions are bridged by H398-L399-H400, T2 connects to one T3 by H64-W65-H66 and the other by H398-L399-H400. These bridges between Cu ions can serve as electron transfer (ET) pathways.
		+	Many of the MCOs have sugars on their surface. In this case there are 4 <scene name='70/707393/3fpx_domains_cu_sugar/1'>sugar chains</scene>.
	== Disease ==		== Disease ==

Scot Wherland at 13:11, 19 July 2015

Scot Wherland — Sun, 19 Jul 2015 13:11:31 GMT

←Older revision		Revision as of 13:11, 19 July 2015
Line 9:		Line 9:
	<scene name='70/707393/3fpx_domains_cu_2ndry/1'>structure</scene>. Adding the		<scene name='70/707393/3fpx_domains_cu_2ndry/1'>structure</scene>. Adding the
	<scene name='70/707393/3fpx_domains_cu_ligands/4'>ligands</scene> to the Cu ions shows that they come from different domains. The ligands of the T1 Cu ion (His-395 and 458 and Cys-453) all come from the yellow domain 3. The ligands of one T3 Cu ion come from the yellow domain 3 (His-400 and 452) and the blue domain 1(His 111). The ligands of the other T3 Cu ion also come from the yellow and blue domains 3 and 1, but two (His-66 and 109) come from the blue domain 1 and only one from the yellow domain 3 (His-454). The ligands of the T2 Cu ion come from the blue domain 1 (His 64) and yellow domain 3 (His-398) and in addition there are three water molecules, one in the center of the TNC, one bridging the T3 Cu ions, and one just associated with the T2 Cu ion. None of the ligands come from the blue domain 2, the middle of the sequence. The ligands can be seen better without the surrounding chains, and with the bridging		<scene name='70/707393/3fpx_domains_cu_ligands/4'>ligands</scene> to the Cu ions shows that they come from different domains. The ligands of the T1 Cu ion (His-395 and 458 and Cys-453) all come from the yellow domain 3. The ligands of one T3 Cu ion come from the yellow domain 3 (His-400 and 452) and the blue domain 1(His 111). The ligands of the other T3 Cu ion also come from the yellow and blue domains 3 and 1, but two (His-66 and 109) come from the blue domain 1 and only one from the yellow domain 3 (His-454). The ligands of the T2 Cu ion come from the blue domain 1 (His 64) and yellow domain 3 (His-398) and in addition there are three water molecules, one in the center of the TNC, one bridging the T3 Cu ions, and one just associated with the T2 Cu ion. None of the ligands come from the blue domain 2, the middle of the sequence. The ligands can be seen better without the surrounding chains, and with the bridging
-	<scene name='70/707393/3fpx_domains_cu_ligands_links/2'>bridging</scene> residues between ligands (Trp-65, Ser-110, and Leu-399).	+	<scene name='70/707393/3fpx_domains_cu_ligands_links/2'>bridging</scene> residues between ligands (Trp-65, Ser-110, and Leu-399. The Cys-453 ligand of T1 connects directly to each of the T3 ions with a His ligand using the H452-C453-H454 segment. The two T3 ions are bridged by H398-L399-H400, T2 connects to one T3 by H64-W65-H66 and the other by H398-L399-H400.
	== Disease ==		== Disease ==

Scot Wherland at 12:50, 19 July 2015

Scot Wherland — Sun, 19 Jul 2015 12:50:25 GMT

←Older revision		Revision as of 12:50, 19 July 2015
Line 1:		Line 1:
-	==Introduction==
	<StructureSection load='3FPX' size='400' side='right' caption='Multicopper Oxidases' scene=''>		<StructureSection load='3FPX' size='400' side='right' caption='Multicopper Oxidases' scene=''>
		+	==Introduction==
	The multicopper oxidases catalyze the reduction of oxygen to water, using a broad variety of substrates. They share a similar set of copper centers the type 1 (T1) or "blue copper" site..		The multicopper oxidases catalyze the reduction of oxygen to water, using a broad variety of substrates. They share a similar set of copper centers the type 1 (T1) or "blue copper" site..

Scot Wherland at 12:45, 19 July 2015

Scot Wherland — Sun, 19 Jul 2015 12:45:51 GMT

←Older revision		Revision as of 12:45, 19 July 2015
Line 6:		Line 6:
	== Laccase from Trametes hirsuta ==		== Laccase from Trametes hirsuta ==
	The laccase from trametes hirsuta has a single polypeptide chain with 3		The laccase from trametes hirsuta has a single polypeptide chain with 3
-	<scene name='70/707393/3fpx_domains_cu/3'>domains</scene> colored green, blue, and yellow. The copper ions are represented by maroon spheres. The domains each form a beta barrel, illustrated when the structure is color coded by secondary	+	<scene name='70/707393/3fpx_domains_cu/4'>domains</scene> colored green, blue, and yellow. The copper ions are represented by maroon spheres. The domains each form a beta barrel, illustrated when the structure is color coded by secondary
	<scene name='70/707393/3fpx_domains_cu_2ndry/1'>structure</scene>. Adding the		<scene name='70/707393/3fpx_domains_cu_2ndry/1'>structure</scene>. Adding the
	<scene name='70/707393/3fpx_domains_cu_ligands/4'>ligands</scene> to the Cu ions shows that they come from different domains. The ligands of the T1 Cu ion (His-395 and 458 and Cys-453) all come from the yellow domain 3. The ligands of one T3 Cu ion come from the yellow domain 3 (His-400 and 452) and the blue domain 1(His 111). The ligands of the other T3 Cu ion also come from the yellow and blue domains 3 and 1, but two (His-66 and 109) come from the blue domain 1 and only one from the yellow domain 3 (His-454). The ligands of the T2 Cu ion come from the blue domain 1 (His 64) and yellow domain 3 (His-398) and in addition there are three water molecules, one in the center of the TNC, one bridging the T3 Cu ions, and one just associated with the T2 Cu ion. None of the ligands come from the blue domain 2, the middle of the sequence. The ligands can be seen better without the surrounding chains, and with the bridging		<scene name='70/707393/3fpx_domains_cu_ligands/4'>ligands</scene> to the Cu ions shows that they come from different domains. The ligands of the T1 Cu ion (His-395 and 458 and Cys-453) all come from the yellow domain 3. The ligands of one T3 Cu ion come from the yellow domain 3 (His-400 and 452) and the blue domain 1(His 111). The ligands of the other T3 Cu ion also come from the yellow and blue domains 3 and 1, but two (His-66 and 109) come from the blue domain 1 and only one from the yellow domain 3 (His-454). The ligands of the T2 Cu ion come from the blue domain 1 (His 64) and yellow domain 3 (His-398) and in addition there are three water molecules, one in the center of the TNC, one bridging the T3 Cu ions, and one just associated with the T2 Cu ion. None of the ligands come from the blue domain 2, the middle of the sequence. The ligands can be seen better without the surrounding chains, and with the bridging

Scot Wherland at 12:42, 19 July 2015

Scot Wherland — Sun, 19 Jul 2015 12:42:07 GMT

←Older revision		Revision as of 12:42, 19 July 2015
Line 6:		Line 6:
	== Laccase from Trametes hirsuta ==		== Laccase from Trametes hirsuta ==
	The laccase from trametes hirsuta has a single polypeptide chain with 3		The laccase from trametes hirsuta has a single polypeptide chain with 3
-	<scene name='70/707393/3fpx_domains_cu/3'>domains</scene> colored green, blue, and yellow. The copper ions are represented by maroon spheres. Adding the	+	<scene name='70/707393/3fpx_domains_cu/3'>domains</scene> colored green, blue, and yellow. The copper ions are represented by maroon spheres. The domains each form a beta barrel, illustrated when the structure is color coded by secondary
		+	<scene name='70/707393/3fpx_domains_cu_2ndry/1'>structure</scene>. Adding the
	<scene name='70/707393/3fpx_domains_cu_ligands/4'>ligands</scene> to the Cu ions shows that they come from different domains. The ligands of the T1 Cu ion (His-395 and 458 and Cys-453) all come from the yellow domain 3. The ligands of one T3 Cu ion come from the yellow domain 3 (His-400 and 452) and the blue domain 1(His 111). The ligands of the other T3 Cu ion also come from the yellow and blue domains 3 and 1, but two (His-66 and 109) come from the blue domain 1 and only one from the yellow domain 3 (His-454). The ligands of the T2 Cu ion come from the blue domain 1 (His 64) and yellow domain 3 (His-398) and in addition there are three water molecules, one in the center of the TNC, one bridging the T3 Cu ions, and one just associated with the T2 Cu ion. None of the ligands come from the blue domain 2, the middle of the sequence. The ligands can be seen better without the surrounding chains, and with the bridging		<scene name='70/707393/3fpx_domains_cu_ligands/4'>ligands</scene> to the Cu ions shows that they come from different domains. The ligands of the T1 Cu ion (His-395 and 458 and Cys-453) all come from the yellow domain 3. The ligands of one T3 Cu ion come from the yellow domain 3 (His-400 and 452) and the blue domain 1(His 111). The ligands of the other T3 Cu ion also come from the yellow and blue domains 3 and 1, but two (His-66 and 109) come from the blue domain 1 and only one from the yellow domain 3 (His-454). The ligands of the T2 Cu ion come from the blue domain 1 (His 64) and yellow domain 3 (His-398) and in addition there are three water molecules, one in the center of the TNC, one bridging the T3 Cu ions, and one just associated with the T2 Cu ion. None of the ligands come from the blue domain 2, the middle of the sequence. The ligands can be seen better without the surrounding chains, and with the bridging
	<scene name='70/707393/3fpx_domains_cu_ligands_links/2'>bridging</scene> residues between ligands (Trp-65, Ser-110, and Leu-399).		<scene name='70/707393/3fpx_domains_cu_ligands_links/2'>bridging</scene> residues between ligands (Trp-65, Ser-110, and Leu-399).

Scot Wherland at 12:17, 19 July 2015

Scot Wherland — Sun, 19 Jul 2015 12:17:16 GMT

←Older revision		Revision as of 12:17, 19 July 2015
Line 7:		Line 7:
	The laccase from trametes hirsuta has a single polypeptide chain with 3		The laccase from trametes hirsuta has a single polypeptide chain with 3
	<scene name='70/707393/3fpx_domains_cu/3'>domains</scene> colored green, blue, and yellow. The copper ions are represented by maroon spheres. Adding the		<scene name='70/707393/3fpx_domains_cu/3'>domains</scene> colored green, blue, and yellow. The copper ions are represented by maroon spheres. Adding the
-	<scene name='70/707393/3fpx_domains_cu_ligands/3'>ligands</scene> to the Cu ions shows that they come from different domains. The ligands of the T1 Cu ion (His-395 and 458 and Cys-453) all come from the yellow domain 3. The ligands of one T3 Cu ion come from the yellow domain 3 (His-400 and 452) and the blue domain 1(His 111). The ligands of the other T3 Cu ion also come from the yellow and blue domains 3 and 1, but two (His-66 and 109) come from the blue domain 1 and only one from the yellow domain 3 (His-454). The ligands of the T2 Cu ion come from the blue domain 1 (His 64) and yellow domain 3 (His-398) and in addition there are three water molecules, one in the center of the TNC, one bridging the T3 Cu ions, and one just associated with the T2 Cu ion. None of the ligands come from the blue domain 2, the middle of the sequence. The ligands can be seen better without the surrounding chains, and with the bridging	+	<scene name='70/707393/3fpx_domains_cu_ligands/4'>ligands</scene> to the Cu ions shows that they come from different domains. The ligands of the T1 Cu ion (His-395 and 458 and Cys-453) all come from the yellow domain 3. The ligands of one T3 Cu ion come from the yellow domain 3 (His-400 and 452) and the blue domain 1(His 111). The ligands of the other T3 Cu ion also come from the yellow and blue domains 3 and 1, but two (His-66 and 109) come from the blue domain 1 and only one from the yellow domain 3 (His-454). The ligands of the T2 Cu ion come from the blue domain 1 (His 64) and yellow domain 3 (His-398) and in addition there are three water molecules, one in the center of the TNC, one bridging the T3 Cu ions, and one just associated with the T2 Cu ion. None of the ligands come from the blue domain 2, the middle of the sequence. The ligands can be seen better without the surrounding chains, and with the bridging
	<scene name='70/707393/3fpx_domains_cu_ligands_links/2'>bridging</scene> residues between ligands (Trp-65, Ser-110, and Leu-399).		<scene name='70/707393/3fpx_domains_cu_ligands_links/2'>bridging</scene> residues between ligands (Trp-65, Ser-110, and Leu-399).
	== Disease ==		== Disease ==

Scot Wherland at 12:13, 19 July 2015

Scot Wherland — Sun, 19 Jul 2015 12:13:28 GMT

←Older revision		Revision as of 12:13, 19 July 2015
Line 8:		Line 8:
	<scene name='70/707393/3fpx_domains_cu/3'>domains</scene> colored green, blue, and yellow. The copper ions are represented by maroon spheres. Adding the		<scene name='70/707393/3fpx_domains_cu/3'>domains</scene> colored green, blue, and yellow. The copper ions are represented by maroon spheres. Adding the
	<scene name='70/707393/3fpx_domains_cu_ligands/3'>ligands</scene> to the Cu ions shows that they come from different domains. The ligands of the T1 Cu ion (His-395 and 458 and Cys-453) all come from the yellow domain 3. The ligands of one T3 Cu ion come from the yellow domain 3 (His-400 and 452) and the blue domain 1(His 111). The ligands of the other T3 Cu ion also come from the yellow and blue domains 3 and 1, but two (His-66 and 109) come from the blue domain 1 and only one from the yellow domain 3 (His-454). The ligands of the T2 Cu ion come from the blue domain 1 (His 64) and yellow domain 3 (His-398) and in addition there are three water molecules, one in the center of the TNC, one bridging the T3 Cu ions, and one just associated with the T2 Cu ion. None of the ligands come from the blue domain 2, the middle of the sequence. The ligands can be seen better without the surrounding chains, and with the bridging		<scene name='70/707393/3fpx_domains_cu_ligands/3'>ligands</scene> to the Cu ions shows that they come from different domains. The ligands of the T1 Cu ion (His-395 and 458 and Cys-453) all come from the yellow domain 3. The ligands of one T3 Cu ion come from the yellow domain 3 (His-400 and 452) and the blue domain 1(His 111). The ligands of the other T3 Cu ion also come from the yellow and blue domains 3 and 1, but two (His-66 and 109) come from the blue domain 1 and only one from the yellow domain 3 (His-454). The ligands of the T2 Cu ion come from the blue domain 1 (His 64) and yellow domain 3 (His-398) and in addition there are three water molecules, one in the center of the TNC, one bridging the T3 Cu ions, and one just associated with the T2 Cu ion. None of the ligands come from the blue domain 2, the middle of the sequence. The ligands can be seen better without the surrounding chains, and with the bridging
-	<scene name='70/707393/3fpx_domains_cu_ligands_links/1'>~~residues~~</scene> between ligands (Trp-65, Ser-110, and Leu-399).	+	<scene name='70/707393/3fpx_domains_cu_ligands_links/2'>bridging</scene> residues between ligands (Trp-65, Ser-110, and Leu-399).
	== Disease ==		== Disease ==

Scot Wherland at 12:09, 19 July 2015

Scot Wherland — Sun, 19 Jul 2015 12:09:41 GMT

←Older revision		Revision as of 12:09, 19 July 2015
Line 7:		Line 7:
	The laccase from trametes hirsuta has a single polypeptide chain with 3		The laccase from trametes hirsuta has a single polypeptide chain with 3
	<scene name='70/707393/3fpx_domains_cu/3'>domains</scene> colored green, blue, and yellow. The copper ions are represented by maroon spheres. Adding the		<scene name='70/707393/3fpx_domains_cu/3'>domains</scene> colored green, blue, and yellow. The copper ions are represented by maroon spheres. Adding the
-	<scene name='70/707393/3fpx_domains_cu_ligands/3'>ligands</scene> to the Cu ions shows that they come from different domains. The ligands of the T1 Cu ion (His-395 and 458 and Cys-453) all come from the yellow domain 3. The ligands of one T3 Cu ion come from the yellow domain 3 (His-400 and 452) and the blue domain 1(His 111). The ligands of the other T3 Cu ion also come from the yellow and blue domains 3 and 1, but two (His-66 and 109) come from the blue domain 1 and only one from the yellow domain 3 (His-454). The ligands of the T2 Cu ion come from the blue domain 1 (His 64) and yellow domain 3 (His-398) and in addition there are three water molecules, one in the center of the TNC, one bridging the T3 Cu ions, and one just associated with the T2 Cu ion. None of the ligands come from the blue domain 2, the middle of the sequence. The ligands can be seen better without the surrounding chains.	+	<scene name='70/707393/3fpx_domains_cu_ligands/3'>ligands</scene> to the Cu ions shows that they come from different domains. The ligands of the T1 Cu ion (His-395 and 458 and Cys-453) all come from the yellow domain 3. The ligands of one T3 Cu ion come from the yellow domain 3 (His-400 and 452) and the blue domain 1(His 111). The ligands of the other T3 Cu ion also come from the yellow and blue domains 3 and 1, but two (His-66 and 109) come from the blue domain 1 and only one from the yellow domain 3 (His-454). The ligands of the T2 Cu ion come from the blue domain 1 (His 64) and yellow domain 3 (His-398) and in addition there are three water molecules, one in the center of the TNC, one bridging the T3 Cu ions, and one just associated with the T2 Cu ion. None of the ligands come from the blue domain 2, the middle of the sequence. The ligands can be seen better without the surrounding chains, and with the bridging
		+	<scene name='70/707393/3fpx_domains_cu_ligands_links/1'>residues</scene> between ligands (Trp-65, Ser-110, and Leu-399).
	== Disease ==		== Disease ==