TRNA-guanine transglycosylase
From Proteopedia
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<StructureSection load='' size='400' side='right' caption='Structure of tRNA-guanine transglycosylase complex with queuine precursor and Zn+2 ion (grey) (PDB entry [[1p0e]])' scene='55/551184/Cv/1'> | <StructureSection load='' size='400' side='right' caption='Structure of tRNA-guanine transglycosylase complex with queuine precursor and Zn+2 ion (grey) (PDB entry [[1p0e]])' scene='55/551184/Cv/1'> | ||
| - | '''tRNA-guanine transglycosylase''' (TGT) is involved in the post-transcriptional modification of tRNA. TGT is involved in the replacement of guanine-34 by queuine precursor (preQ1) in the anticodon of tRNA<ref>PMID:11680848</ref>. In archaebacteria TGT (ATGT) catalyzes the introduction of archaeosin precursor (preQ0) into the D-loop of tRNA<ref>PMID:16407303</ref>. | + | '''tRNA-guanine transglycosylase''' (TGT) or '''queuine tRNA-ribosyltransferase''' is involved in the post-transcriptional modification of tRNA. TGT is involved in the replacement of guanine-34 by queuine precursor (preQ1) in the anticodon of tRNA<ref>PMID:11680848</ref>. In archaebacteria TGT (ATGT) catalyzes the introduction of archaeosin precursor (preQ0) into the D-loop of tRNA<ref>PMID:16407303</ref>. |
== Structural highlights == | == Structural highlights == | ||
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**[[1wkd]], [[1wke]], [[1wkf]], [[1ozm]], [[2oko]], [[2z1v]], [[2nso]], [[3bl3]], [[3hfy]], [[4gd0]], [[4h6e]], [[3unt]], [[3uvi]], [[4dxx]], [[4dy1]], [[4htb]], [[4ipp]], [[4jbr]], [[4l56]], [[4q8m]], [[4q8n]] – ZmTGT (mutant)<BR /> | **[[1wkd]], [[1wke]], [[1wkf]], [[1ozm]], [[2oko]], [[2z1v]], [[2nso]], [[3bl3]], [[3hfy]], [[4gd0]], [[4h6e]], [[3unt]], [[3uvi]], [[4dxx]], [[4dy1]], [[4htb]], [[4ipp]], [[4jbr]], [[4l56]], [[4q8m]], [[4q8n]] – ZmTGT (mutant)<BR /> | ||
**[[2ash]] – TGT – ''Thermotoga maritima''<BR /> | **[[2ash]] – TGT – ''Thermotoga maritima''<BR /> | ||
| + | **[[6h42]] – hTGT catalytic subunit – human<BR /> | ||
| + | **[[6fv5]] – TGT non-catalytic subunit – mouse<BR /> | ||
*tRNA-guanine transglycosylase complexes | *tRNA-guanine transglycosylase complexes | ||
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**[[1enu]], [[1f3e]] – ZmTGT + aminophthalhydrazide<BR /> | **[[1enu]], [[1f3e]] – ZmTGT + aminophthalhydrazide<BR /> | ||
**[[1r5y]], [[1q4w]], [[1q63]], [[1q65]], [[1q66]], [[1s38]], [[1s39]], [[1y5v]], [[1y5w]], [[1y5x]], [[2bbf]], [[2qzr]], [[3eos]], [[3eou]], [[3gc4]], [[1k4g]], [[1k4h]], [[3gc5]], [[3ge7]], [[4puj]], [[4puk]], [[4q4o]], [[4q4p]], [[4q4q]], [[4q4r]], [[4q4s]], [[4q8t]], [[4q4u]], [[4q8v]], [[4q8w]], [[5jxq]], [[5jsw]], [[5jxq]], [[5lpo]], [[5lpp]], [[5lpq]], [[5lps]], [[5lpt]] – ZmTGT + quinazoline inhibitor<BR /> | **[[1r5y]], [[1q4w]], [[1q63]], [[1q65]], [[1q66]], [[1s38]], [[1s39]], [[1y5v]], [[1y5w]], [[1y5x]], [[2bbf]], [[2qzr]], [[3eos]], [[3eou]], [[3gc4]], [[1k4g]], [[1k4h]], [[3gc5]], [[3ge7]], [[4puj]], [[4puk]], [[4q4o]], [[4q4p]], [[4q4q]], [[4q4r]], [[4q4s]], [[4q8t]], [[4q4u]], [[4q8v]], [[4q8w]], [[5jxq]], [[5jsw]], [[5jxq]], [[5lpo]], [[5lpp]], [[5lpq]], [[5lps]], [[5lpt]] – ZmTGT + quinazoline inhibitor<BR /> | ||
| - | **[[4q4m]] – ZmTGT + triazine inhibitor<BR /> | ||
**[[4pul]], [[4pum]], [[4q8o]], [[4q8p]], [[4q8q]] – ZmTGT (mutant) + quinazoline inhibitor<BR /> | **[[4pul]], [[4pum]], [[4q8o]], [[4q8p]], [[4q8q]] – ZmTGT (mutant) + quinazoline inhibitor<BR /> | ||
| + | **[[4q4m]] – ZmTGT + triazine inhibitor<BR /> | ||
**[[1n2v]], [[2z1w]] – ZmTGT + pyridazine derivative<BR /> | **[[1n2v]], [[2z1w]] – ZmTGT + pyridazine derivative<BR /> | ||
**[[5sw3]] – ZmTGT + pyridine derivative<BR /> | **[[5sw3]] – ZmTGT + pyridine derivative<BR /> | ||
| + | **[[5utj]] – ZmTGT + azapurine derivative<BR /> | ||
| + | **[[5v3c]] – ZmTGT + cyclohexane derivative<BR /> | ||
**[[1q2r]], [[1q2s]] – ZmTGT + RNA<BR /> | **[[1q2r]], [[1q2s]] – ZmTGT + RNA<BR /> | ||
**[[2pot]], [[2pwu]], [[4h7z]] – ZmTGT (mutant) + guanine<BR /> | **[[2pot]], [[2pwu]], [[4h7z]] – ZmTGT (mutant) + guanine<BR /> | ||
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**[[5j9m]], [[5j9n]], [[5j9o]], [[5jt5]], [[5jt6]], [[5jt7]] – ZmTGT + benzoimidazole derivative<BR /> | **[[5j9m]], [[5j9n]], [[5j9o]], [[5jt5]], [[5jt6]], [[5jt7]] – ZmTGT + benzoimidazole derivative<BR /> | ||
**[[3s1g]], [[3sm0]], [[4gg9]], [[4gh1]], [[4gh3]], [[4ghr]] – ZmTGT + benzohypoxanthine derivative<BR /> | **[[3s1g]], [[3sm0]], [[4gg9]], [[4gh1]], [[4gh3]], [[4ghr]] – ZmTGT + benzohypoxanthine derivative<BR /> | ||
| + | **[[6h45]] – hTGT + queuine<BR /> | ||
*Archaebacterial tRNA-guanine transglycosylase | *Archaebacterial tRNA-guanine transglycosylase | ||
Revision as of 20:46, 27 September 2018
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3D structures of tRNA-guanine transglycosylase
Updated on 27-September-2018
References
- ↑ Nonekowski ST, Garcia GA. tRNA recognition by tRNA-guanine transglycosylase from Escherichia coli: the role of U33 in U-G-U sequence recognition. RNA. 2001 Oct;7(10):1432-41. PMID:11680848
- ↑ Sabina J, Soll D. The RNA-binding PUA domain of archaeal tRNA-guanine transglycosylase is not required for archaeosine formation. J Biol Chem. 2006 Mar 17;281(11):6993-7001. Epub 2006 Jan 10. PMID:16407303 doi:http://dx.doi.org/M512841200
- ↑ Brenk R, Stubbs MT, Heine A, Reuter K, Klebe G. Flexible adaptations in the structure of the tRNA-modifying enzyme tRNA-guanine transglycosylase and their implications for substrate selectivity, reaction mechanism and structure-based drug design. Chembiochem. 2003 Oct 6;4(10):1066-77. PMID:14523925 doi:10.1002/cbic.200300644
