TRNA-guanine transglycosylase
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
TGT overall structure is of a <scene name='55/551184/Cv/5'>Tim barrel</scene>. A structural <scene name='55/551184/Cv/6'>Zn+2 ion is tetrahedrally coordinated</scene> in the C-terminal subdomain. The <scene name='55/551184/Cv/7'>active site contains queuine precursor</scene><ref>PMID:14523925</ref>. | TGT overall structure is of a <scene name='55/551184/Cv/5'>Tim barrel</scene>. A structural <scene name='55/551184/Cv/6'>Zn+2 ion is tetrahedrally coordinated</scene> in the C-terminal subdomain. The <scene name='55/551184/Cv/7'>active site contains queuine precursor</scene><ref>PMID:14523925</ref>. | ||
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| + | ==3D structures of tRNA-guanine transglycosylase== | ||
| + | [[tRNA-guanine transglycosylase 3D structures]] | ||
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</StructureSection> | </StructureSection> | ||
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**[[6h42]] – hTGT catalytic subunit – human<BR /> | **[[6h42]] – hTGT catalytic subunit – human<BR /> | ||
**[[6fv5]] – mTGT non-catalytic subunit – mouse<BR /> | **[[6fv5]] – mTGT non-catalytic subunit – mouse<BR /> | ||
| + | **[[7b2i]] – mTGT catalytic + accessory subunits (mutant)<BR /> | ||
**[[6h62]] – mTGT catalytic subunit <BR /> | **[[6h62]] – mTGT catalytic subunit <BR /> | ||
**[[1pud]], [[1p0d]], [[4pun]] – ZmTGT – ''Zymomonas mobilis''<BR /> | **[[1pud]], [[1p0d]], [[4pun]] – ZmTGT – ''Zymomonas mobilis''<BR /> | ||
| - | **[[1wkd]], [[1wke]], [[1wkf]], [[1ozm]], [[2oko]], [[2z1v]], [[2nso]], [[3bl3]], [[3hfy]], [[4gd0]], [[4h6e]], [[3unt]], [[3uvi]], [[4dxx]], [[4dy1]], [[4htb]], [[4ipp]], [[4jbr]], [[4l56]], [[4q8m]], [[4q8n]], [[6h7c]], [[6ygm]], [[6yry]], [[6yfx]], [[6yfw]], [[6z0d]], [[7apm]], [[7apl]] – ZmTGT (mutant)<BR /> | + | **[[1wkd]], [[1wke]], [[1wkf]], [[1ozm]], [[2oko]], [[2z1v]], [[2nso]], [[3bl3]], [[3hfy]], [[4gd0]], [[4h6e]], [[3unt]], [[3uvi]], [[4dxx]], [[4dy1]], [[4htb]], [[4ipp]], [[4jbr]], [[4l56]], [[4q8m]], [[4q8n]], [[6h7c]], [[6ygm]], [[6yry]], [[6yfx]], [[6yfw]], [[6z0d]], [[7a0b]], [[7a3v]], [[7a3x]], [[7a4k]], [[7a4x]], [[7a6d]], [[7a9e]], [[7adn]], [[7apm]], [[7apl]] – ZmTGT (mutant)<BR /> |
**[[2ash]] – TGT – ''Thermotoga maritima''<BR /> | **[[2ash]] – TGT – ''Thermotoga maritima''<BR /> | ||
*tRNA-guanine transglycosylase complexes | *tRNA-guanine transglycosylase complexes | ||
| + | **[[6h45]] – hTGT + queuine<BR /> | ||
| + | **[[7nq4]] – hTGT catalytic + accessory subunits + RNA<BR /> | ||
**[[1p0e]] – ZmTGT + preQ1<BR /> | **[[1p0e]] – ZmTGT + preQ1<BR /> | ||
**[[1efz]], [[1pxg]], [[1ozq]], [[2z1x]], [[2nqz]], [[3bld]], [[4gcx]], [[4e2v]] – ZmTGT (mutant) + preQ1<BR /> | **[[1efz]], [[1pxg]], [[1ozq]], [[2z1x]], [[2nqz]], [[3bld]], [[4gcx]], [[4e2v]] – ZmTGT (mutant) + preQ1<BR /> | ||
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**[[5j9m]], [[5j9n]], [[5j9o]], [[5jt5]], [[5jt6]], [[5jt7]] – ZmTGT + benzoimidazole derivative<BR /> | **[[5j9m]], [[5j9n]], [[5j9o]], [[5jt5]], [[5jt6]], [[5jt7]] – ZmTGT + benzoimidazole derivative<BR /> | ||
**[[3s1g]], [[3sm0]], [[4gg9]], [[4gh1]], [[4gh3]], [[4ghr]] – ZmTGT + benzohypoxanthine derivative<BR /> | **[[3s1g]], [[3sm0]], [[4gg9]], [[4gh1]], [[4gh3]], [[4ghr]] – ZmTGT + benzohypoxanthine derivative<BR /> | ||
| - | **[[6h45]] – hTGT + queuine<BR /> | ||
*Archaebacterial tRNA-guanine transglycosylase | *Archaebacterial tRNA-guanine transglycosylase | ||
Revision as of 09:01, 18 May 2022
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3D structures of tRNA-guanine transglycosylase
Updated on 18-May-2022
References
- ↑ Nonekowski ST, Garcia GA. tRNA recognition by tRNA-guanine transglycosylase from Escherichia coli: the role of U33 in U-G-U sequence recognition. RNA. 2001 Oct;7(10):1432-41. PMID:11680848
- ↑ Sabina J, Soll D. The RNA-binding PUA domain of archaeal tRNA-guanine transglycosylase is not required for archaeosine formation. J Biol Chem. 2006 Mar 17;281(11):6993-7001. Epub 2006 Jan 10. PMID:16407303 doi:http://dx.doi.org/M512841200
- ↑ Brenk R, Stubbs MT, Heine A, Reuter K, Klebe G. Flexible adaptations in the structure of the tRNA-modifying enzyme tRNA-guanine transglycosylase and their implications for substrate selectivity, reaction mechanism and structure-based drug design. Chembiochem. 2003 Oct 6;4(10):1066-77. PMID:14523925 doi:10.1002/cbic.200300644
