TRNA-guanine transglycosylase

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<StructureSection load='1p0e' size='350' side='right' caption='Structure of tRNA-guanine transglycosylase complex with queuine precursor (PDB entry [[1p0e]])' scene=''>
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<StructureSection load='' size='400' side='right' caption='Structure of tRNA-guanine transglycosylase complex with queuine precursor and Zn+2 ion (grey) (PDB entry [[1p0e]])' scene='55/551184/Cv/1'>
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'''tRNA-guanine transglycosylase''' (TGT) is involved in the post-transcriptional modification of tRNA. TGT is involved in the replacement of guanine-34 by queuine precursor (preQ1) in the anticodon of tRNA<ref>PMID:11680848</ref>. In archaebacteria TGT (ATGT) catalyzes the introduction of archaeosin precursor (preQ0) into the D-loop of tRNA.
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'''tRNA-guanine transglycosylase''' (TGT) or '''queuine tRNA-ribosyltransferase''' is involved in the post-transcriptional modification of tRNA. TGT is involved in the replacement of guanine-34 by queuine precursor (preQ1) in the anticodon of tRNA<ref>PMID:11680848</ref>. In archaebacteria TGT (ATGT) catalyzes the introduction of archaeosin precursor (preQ0) into the D-loop of tRNA<ref>PMID:16407303</ref>.
== Structural highlights ==
== Structural highlights ==
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TGT overall structure is of a Tim barrel. A structural Zn+2 ion is tetrahedrally coordinated in the C-terminal subdomain. The active site contains preQ1<ref>PMID:14523925</ref>.
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TGT overall structure is of a <scene name='55/551184/Cv/5'>Tim barrel</scene>. A structural <scene name='55/551184/Cv/6'>Zn+2 ion is tetrahedrally coordinated</scene> in the C-terminal subdomain. The <scene name='55/551184/Cv/7'>active site contains queuine precursor</scene><ref>PMID:14523925</ref>.
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</StructureSection>
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==3D structures of tRNA-guanine transglycosylase==
==3D structures of tRNA-guanine transglycosylase==
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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[[tRNA-guanine transglycosylase 3D structures]]
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*tRNA-guanine transglycosylase
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</StructureSection>
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**[[1pud]], [[1p0d]], [[4pun]] – ZmTGT – ''Zymomonas mobilis''<BR />
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**[[1wkd]], [[1wke]], [[1wkf]], [[1ozm]], [[2oko]], [[2z1v]], [[2nso]], [[3bl3]], [[3hfy]], [[4gd0]], [[4h6e]], [[3unt]], [[3uvi]], [[4dxx]], [[4dy1]], [[4htb]], [[4ipp]], [[4jbr]], [[4l56]], [[4q8m]], [[4q8n]] – ZmTGT (mutant)<BR />
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**[[2ash]] – TGT – ''Thermotoga maritima''<BR />
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*tRNA-guanine transglycosylase complexes
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**[[1efz]], [[1pxg]], [[1ozq]], [[2z1x]], [[2nqz]], [[3bld]], [[4gcx]], [[4e2v]] – ZmTGT (mutant) + preQ1<BR />
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**[[3bll]] – ZmTGT (mutant) + preQ1 derivative<BR />
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**[[1p0e]] – ZmTGT + preQ1<BR />
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**[[1p0b]], [[2qii]] – ZmTGT + preQ0<BR />
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**[[2pwv]] – ZmTGT (mutant) + preQ0<BR />
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**[[3blo]], [[4hqv]], [[4hsh]], [[4hvx]] – ZmTGT (mutant) + queuine<BR />
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**[[1enu]], [[1f3e]] – ZmTGT + aminophthalhydrazide<BR />
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**[[1r5y]], [[1q4w]], [[1q63]], [[1q65]], [[1q66]], [[1s38]], [[1s39]], [[1y5v]], [[1y5w]], [[1y5x]], [[2bbf]], [[2qzr]], [[3c2n]], [[3c2y]], [[3c2z]], [[3eos]], [[3eou]], [[3gc4]], [[1k4g]], [[1k4h]], [[3gc5]], [[3ge7]], [[3gev]], [[4puj]], [[4puk]], [[4q4o]], [[4q4p]], [[4q4q]], [[4q4r]], [[4q4s]], [[4q8t]], [[4q4u]], [[4q8v]], [[4q8w]], [[5jxq]] – ZmTGT + quinazoline inhibitor<BR />
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**[[4q4m]] – ZmTGT + triazine inhibitor<BR />
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**[[4pul]], [[4pum]], [[4q8o]], [[4q8p]], [[4q8q]] – ZmTGT (mutant) + quinazoline inhibitor<BR />
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**[[1n2v]], [[2z1w]] – ZmTGT + pyridazine derivative<BR />
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**[[1q2r]], [[1q2s]] – ZmTGT + RNA<BR />
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**[[2pot]], [[2pwu]], [[4h7z]] – ZmTGT (mutant) + guanine<BR />
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**[[2z7k]], [[3rr4]], [[3tll]], [[4fr6]], [[4fsa]], [[4fr1]], [[4fps]], [[4gi4]], [[4giy]], [[4gkt]], [[4kwo]], [[4lbu]], [[4leq]] – ZmTGT + benzoguanine derivative<BR />
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**[[3v0y]], [[3s1g]], [[3sm0]], [[4gg9]], [[4gh1]], [[4gh3]], [[4ghr]] – ZmTGT + benzohypoxanthine derivative<BR />
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*Archaebacterial tRNA-guanine transglycosylase
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**[[1iq8]] – PhATGT – ''Pyrococcus horikoshii''<BR />
 
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**[[1it7]] – PhATGT + guanine<BR />
 
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**[[1it8]] – PhATGT + preQ0<BR />
 
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**[[1j2b]] – PhATGT + tRNA-Val<BR />
 
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}}
 
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Structure of tRNA-guanine transglycosylase complex with queuine precursor and Zn+2 ion (grey) (PDB entry 1p0e)

Drag the structure with the mouse to rotate

References

  1. ↑ Nonekowski ST, Garcia GA. tRNA recognition by tRNA-guanine transglycosylase from Escherichia coli: the role of U33 in U-G-U sequence recognition. RNA. 2001 Oct;7(10):1432-41. PMID:11680848
  2. ↑ Sabina J, Soll D. The RNA-binding PUA domain of archaeal tRNA-guanine transglycosylase is not required for archaeosine formation. J Biol Chem. 2006 Mar 17;281(11):6993-7001. Epub 2006 Jan 10. PMID:16407303 doi:http://dx.doi.org/M512841200
  3. ↑ Brenk R, Stubbs MT, Heine A, Reuter K, Klebe G. Flexible adaptations in the structure of the tRNA-modifying enzyme tRNA-guanine transglycosylase and their implications for substrate selectivity, reaction mechanism and structure-based drug design. Chembiochem. 2003 Oct 6;4(10):1066-77. PMID:14523925 doi:10.1002/cbic.200300644

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Michal Harel, Alexander Berchansky, Joel L. Sussman

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