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2eh7

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{{Seed}}
 
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[[Image:2eh7.png|left|200px]]
 
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==Crystal structure of humanized KR127 FAB==
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The line below this paragraph, containing "STRUCTURE_2eh7", creates the "Structure Box" on the page.
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<StructureSection load='2eh7' size='340' side='right'caption='[[2eh7]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2eh7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EH7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EH7 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eh7 OCA], [https://pdbe.org/2eh7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eh7 RCSB], [https://www.ebi.ac.uk/pdbsum/2eh7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eh7 ProSAT]</span></td></tr>
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{{STRUCTURE_2eh7| PDB=2eh7 | SCENE= }}
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eh/2eh7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eh7 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The humanized monoclonal antibody HzKR127 recognizes the preS1 domain of the human hepatitis B virus surface proteins with a broadly neutralizing activity in vivo. We present the crystal structures of HzKR127 Fab and its complex with a major epitope peptide. In the complex structure, the bound peptide forms a type IV beta-turn followed by 3(10) helical turn, the looped-out conformation of which provides a structural basis for broad neutralization. Upon peptide binding, the antibody undergoes a dramatic complementarity determining region H3 lid opening. To understand the structural implication of the virus neutralization, we carried out comprehensive alanine-scanning mutagenesis of all complementarity determining region residues in HzKR127 Fab. The functional mapping of the antigen-combining site demonstrates the specific roles of major binding determinants in antigen binding, contributing to the rational design for maximal humanization and affinity maturation of the antibody.
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===Crystal structure of humanized KR127 FAB===
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Broadly neutralizing anti-hepatitis B virus antibody reveals a complementarity determining region H3 lid-opening mechanism.,Chi SW, Maeng CY, Kim SJ, Oh MS, Ryu CJ, Kim SJ, Han KH, Hong HJ, Ryu SE Proc Natl Acad Sci U S A. 2007 May 29;104(22):9230-5. Epub 2007 May 17. PMID:17517649<ref>PMID:17517649</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2eh7" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_17517649}}, adds the Publication Abstract to the page
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*[[Antibody 3D structures|Antibody 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 17517649 is the PubMed ID number.
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*[[Sandbox 20009|Sandbox 20009]]
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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{{ABSTRACT_PUBMED_17517649}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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2EH7 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EH7 OCA].
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</StructureSection>
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[[Category: Large Structures]]
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==Reference==
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<ref group="xtra">PMID:17517649</ref><references group="xtra"/>
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Chi, S W.]]
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[[Category: Chi S-W]]
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[[Category: Hong, H J.]]
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[[Category: Hong HJ]]
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[[Category: Kim, S J.]]
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[[Category: Kim S-J]]
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[[Category: Maeng, C Y.]]
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[[Category: Maeng C-Y]]
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[[Category: Ryu, S E.]]
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[[Category: Ryu S-E]]
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[[Category: Crystal structure]]
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[[Category: Hepatitis b virus]]
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[[Category: Humanized antibody]]
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[[Category: Immune system]]
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[[Category: Monoclonal antibody]]
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[[Category: Neutralization]]
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[[Category: Pres1]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 15:36:27 2009''
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Current revision

Crystal structure of humanized KR127 FAB

PDB ID 2eh7

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