Xylosidase

Function

Xylosidase (XYL) catalyzes the hydrolysis of α- or β-glycosidic linkages.

• α-XYL catalyzes the exo-hydrolysis of 1,4-β-D-glycosidic or exo-hydrolysis of 1,3-β-D-glycosidic linkage from the non-reducing termini of xylans^[1]. XYL catalyzes the conversion of cellulose and hemicellulose xylan to xylose.
• β-XYL catalyzes the endo-hydrolysis of 1,4-β-D-glycosidic or endo-hydrolysis of 1,3-β-D-glycosidic linkage^[2].
• α-XYL family 31 catalyzes the hydrolysis of α-xylosyl from isoprimeverose^[3].
• -XYL catalyzes the endo-hydrolysis of 1,4-β-D-glycosidic or endo-hydrolysis of 1,3-β-D-glycosidic linkage^[4].

Structural highlights

The ^[5]. Water molecules are shown as red spheres. .

3D Structures of xylosidase

Xylosidase 3D structures

References

1. ↑ Moracci M, Cobucci Ponzano B, Trincone A, Fusco S, De Rosa M, van Der Oost J, Sensen CW, Charlebois RL, Rossi M. Identification and molecular characterization of the first alpha -xylosidase from an archaeon. J Biol Chem. 2000 Jul 21;275(29):22082-9. PMID:10801892 doi:http://dx.doi.org/10.1074/jbc.M910392199
2. ↑ Martinez GA, Chaves AR, Civello PM. beta-xylosidase activity and expression of a beta-xylosidase gene during strawberry fruit ripening. Plant Physiol Biochem. 2004 Feb;42(2):89-96. PMID:15283123 doi:http://dx.doi.org/10.1016/j.plaphy.2003.12.001
3. ↑ Nakamichi Y, Matsuzawa T, Watanabe M, Yaoi K. Crystal structure of glycoside hydrolase family 31 α-xylosidase from a soil metagenome. Biosci Biotechnol Biochem. 2022 Jun 25;86(7):855-864. PMID:35441671 doi:10.1093/bbb/zbac058
4. ↑ Martinez GA, Chaves AR, Civello PM. beta-xylosidase activity and expression of a beta-xylosidase gene during strawberry fruit ripening. Plant Physiol Biochem. 2004 Feb;42(2):89-96. PMID:15283123 doi:http://dx.doi.org/10.1016/j.plaphy.2003.12.001
5. ↑ Larsbrink J, Izumi A, Ibatullin F, Nakhai A, Gilbert HJ, Davies GJ, Brumer H. Structural and enzymatic characterisation of a Glycoside Hydrolase Family 31 alphaxylosidase from Cellvibrio japonicus involved in xyloglucan saccharification. Biochem J. 2011 Mar 22. PMID:21426303 doi:10.1042/BJ20110299