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2h7o

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[[Image:2h7o.gif|left|200px]]
[[Image:2h7o.gif|left|200px]]
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{{Structure
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The line below this paragraph, containing "STRUCTURE_2h7o", creates the "Structure Box" on the page.
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|GENE= ypkA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=633 Yersinia pseudotuberculosis])
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{{STRUCTURE_2h7o| PDB=2h7o | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7o OCA], [http://www.ebi.ac.uk/pdbsum/2h7o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h7o RCSB]</span>
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'''Crystal structure of the Rho-GTPase binding domain of YpkA'''
'''Crystal structure of the Rho-GTPase binding domain of YpkA'''
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[[Category: Prehna, G.]]
[[Category: Prehna, G.]]
[[Category: Stebbins, C E.]]
[[Category: Stebbins, C E.]]
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[[Category: gdi]]
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[[Category: Gdi]]
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[[Category: yersinia]]
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[[Category: Yersinia]]
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[[Category: yopo]]
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[[Category: Yopo]]
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[[Category: ypka]]
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[[Category: Ypka]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:58:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:26:38 2008''
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Revision as of 02:58, 4 May 2008

Image:2h7o.gif

Template:STRUCTURE 2h7o

Crystal structure of the Rho-GTPase binding domain of YpkA


Overview

Yersinia spp. cause gastroenteritis and the plague, representing historically devastating pathogens that are currently an important biodefense and antibiotic resistance concern. A critical virulence determinant is the Yersinia protein kinase A, or YpkA, a multidomain protein that disrupts the eukaryotic actin cytoskeleton. Here we solve the crystal structure of a YpkA-Rac1 complex and find that YpkA possesses a Rac1 binding domain that mimics host guanidine nucleotide dissociation inhibitors (GDIs) of the Rho GTPases. YpkA inhibits nucleotide exchange in Rac1 and RhoA, and mutations that disrupt the YpkA-GTPase interface abolish this activity in vitro and impair in vivo YpkA-induced cytoskeletal disruption. In cell culture experiments, the kinase and the GDI domains of YpkA act synergistically to promote cytoskeletal disruption, and a Y. pseudotuberculosis mutant lacking YpkA GDI activity shows attenuated virulence in a mouse infection assay. We conclude that virulence in Yersinia depends strongly upon mimicry of host GDI proteins by YpkA.

About this Structure

2H7O is a Single protein structure of sequence from Yersinia pseudotuberculosis. Full crystallographic information is available from OCA.

Reference

Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors., Prehna G, Ivanov MI, Bliska JB, Stebbins CE, Cell. 2006 Sep 8;126(5):869-80. PMID:16959567 Page seeded by OCA on Sun May 4 05:58:03 2008

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