1ruk

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{{STRUCTURE_1ruk| PDB=1ruk | SCENE= }}
{{STRUCTURE_1ruk| PDB=1ruk | SCENE= }}
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'''Crystal structure (C) of native cationic cyclization antibody 4C6 fab at pH 4.6 with a data set collected at SSRL beamline 9-1'''
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===Crystal structure (C) of native cationic cyclization antibody 4C6 fab at pH 4.6 with a data set collected at SSRL beamline 9-1===
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==Overview==
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Antibodies can catalyze the generation of hydrogen peroxide (H2O2) from singlet dioxygen (1O2*) and water via the postulated intermediacy of dihydrogen trioxide (H2O3) and other trioxygen species. Nine different crystal structures were determined to elucidate the chemical consequences to the antibody molecule itself of exposure to such reactive intermediates and to provide insights into the location on the antibody where these species could be generated. Herein, we report structural evidence for modifications of two specific antibody residues within the interfacial region of the variable and constant domains of different murine antibody antigen-binding fragments (Fabs) by reactive species generated during the antibody-catalyzed water oxidation process. Crystal structure analyses of murine Fabs 4C6 and 13G5 after UV-irradiation revealed complex oxidative modifications to tryptophan L163 and, in 4C6, hydroxylation of the Cgamma of glutamine H6. These discrete modifications of specific residues add further support for the "active site" of the water-oxidation pathway being located within the interfacial region of the constant and variable domains and highlight the general resistance of the antibody molecule to oxidation by reactive oxygen species generated during the water-oxidation process.
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(as it appears on PubMed at http://www.pubmed.gov), where 14982995 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14982995}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Probing the antibody-catalyzed water-oxidation pathway at atomic resolution., Zhu X, Wentworth P Jr, Wentworth AD, Eschenmoser A, Lerner RA, Wilson IA, Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2247-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14982995 14982995]
Probing the antibody-catalyzed water-oxidation pathway at atomic resolution., Zhu X, Wentworth P Jr, Wentworth AD, Eschenmoser A, Lerner RA, Wilson IA, Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2247-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14982995 14982995]
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Structural basis for antibody catalysis of a cationic cyclization reaction., Zhu X, Heine A, Monnat F, Houk KN, Janda KD, Wilson IA, J Mol Biol. 2003 May 23;329(1):69-83. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12742019 12742019]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Immunoglobulin]]
[[Category: Immunoglobulin]]
[[Category: Water oxidation]]
[[Category: Water oxidation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:55:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:52:25 2008''

Revision as of 11:52, 29 July 2008

Image:1ruk.png

Template:STRUCTURE 1ruk

Crystal structure (C) of native cationic cyclization antibody 4C6 fab at pH 4.6 with a data set collected at SSRL beamline 9-1

Template:ABSTRACT PUBMED 14982995

About this Structure

1RUK is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Probing the antibody-catalyzed water-oxidation pathway at atomic resolution., Zhu X, Wentworth P Jr, Wentworth AD, Eschenmoser A, Lerner RA, Wilson IA, Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2247-52. PMID:14982995

Structural basis for antibody catalysis of a cationic cyclization reaction., Zhu X, Heine A, Monnat F, Houk KN, Janda KD, Wilson IA, J Mol Biol. 2003 May 23;329(1):69-83. PMID:12742019

Page seeded by OCA on Tue Jul 29 14:52:25 2008

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