Resolvase

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(New page: Crystal structure of yeast Holliday junction resolvase, 1kcf {{STRUCTURE_1kcf| PDB=1kcf | SIZE=300| SCENE= |right|CAPTION=yeast Holliday junction ...)
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[[Image:1kcf.png|left|200px|thumb|Crystal structure of yeast Holliday junction resolvase, [[1kcf]]]]
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<StructureSection load='1kcf' size='340' side='right' caption='Yeast Holliday junction resolvase dimer complex with sulfate, [[1kcf]]' scene='' >
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{{STRUCTURE_1kcf| PDB=1kcf | SIZE=300| SCENE= |right|CAPTION=yeast Holliday junction resolvase, [[1kcf]] }}
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== Function ==
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[[Resolvase]] or '''recombinase''' (Rec) is a nuclease which is involved in DNA recombination. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase.
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*'''Holliday junction resolvase''' (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands<ref>PMID:19442245</ref>. <br />
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*'''Tyr-recombinases''' include '''Cre''' which recombines loxP sites, FLP and lambda integrase (LamInt). <br />
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*'''Ser-recombinases''' (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase.<br />
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*'''RadA recombinase''' promotes DNA recombination. Detailed analysis of its <scene name='Resolvase/Cv/2'>dimeric structure</scene> suggests mechanisms for junction isomerization and communication between the two active sites <ref>PMID:19143611</ref>. <br />
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*'''RadB recombinase''' is a paralogue of RadA assisting in RadA-mediated strand exchange <ref>PMID:28501701</ref>. <br />
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*'''Hin-recombinase''' (HRec) is a protein of ''Salmonella''. HRec inverts a 900 base pair DNA segment which contains the promoters of flagellar genes. The inversion changes the these genes' expressions<ref>PMID:15454079</ref>.
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*'''Tre recombinase''' recognizes a target sequence of the HIV virus resulting in excision and eradication of the provirus from infected human cells <ref>PMID:19066582</ref>. <br />
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==3D structures of resolvase==
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[[Resolvase 3D structures]]
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</StructureSection>
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==References==
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<references />
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[[Category:Topic Page]]

Current revision

Yeast Holliday junction resolvase dimer complex with sulfate, 1kcf

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References

  1. ↑ West SC. The search for a human Holliday junction resolvase. Biochem Soc Trans. 2009 Jun;37(Pt 3):519-26. doi: 10.1042/BST0370519. PMID:19442245 doi:http://dx.doi.org/10.1042/BST0370519
  2. ↑ Haldenby S, White MF, Allers T. RecA family proteins in archaea: RadA and its cousins. Biochem Soc Trans. 2009 Feb;37(Pt 1):102-7. doi: 10.1042/BST0370102. PMID:19143611 doi:http://dx.doi.org/10.1042/BST0370102
  3. ↑ Wardell K, Haldenby S, Jones N, Liddell S, Ngo GHP, Allers T. RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator. DNA Repair (Amst). 2017 Jul;55:7-16. PMID:28501701 doi:10.1016/j.dnarep.2017.04.005
  4. ↑ Dhar G, Sanders ER, Johnson RC. Architecture of the hin synaptic complex during recombination: the recombinase subunits translocate with the DNA strands. Cell. 2004 Oct 1;119(1):33-45. PMID:15454079 doi:http://dx.doi.org/10.1016/j.cell.2004.09.010
  5. ↑ Buchholz F. Molecular evolution of the tre recombinase. J Vis Exp. 2008 May 29;(15):791. PMID:19066582 doi:10.3791/791

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Michal Harel, Alexander Berchansky, Joel L. Sussman, Jaime Prilusky

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