Rhodopsin kinase
From Proteopedia
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| - | <StructureSection load=' | + | <StructureSection load='' size='350' side='right' caption='Bovine rhodopsin kinase complex with ATP, Mg+2 and Cl- ions (PDB code [[3c4x]])' scene='70/706261/Cv/1'> |
== Function == | == Function == | ||
'''Rhodopsin kinase''' (RK) is an enzyme which catalyzes the conversion of rhodopsin and ATP to phosphorhodopsin and ADP. RK is retina-specific and the phosphorylation of rhodopsin initiates the phototransduction cascade<ref>PMID:3040978</ref>. The rapid desensitization permits rapid adaptation to changes in illumination. RK is inhibited by the neuronal calcium-binding protein recoverin. | '''Rhodopsin kinase''' (RK) is an enzyme which catalyzes the conversion of rhodopsin and ATP to phosphorhodopsin and ADP. RK is retina-specific and the phosphorylation of rhodopsin initiates the phototransduction cascade<ref>PMID:3040978</ref>. The rapid desensitization permits rapid adaptation to changes in illumination. RK is inhibited by the neuronal calcium-binding protein recoverin. | ||
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== Structural highlights == | == Structural highlights == | ||
| - | The active site of RK is located between its small and large lobes and contains the nucleotide and | + | The active site of RK is located between its <scene name='70/706261/Cv/8'>small and large lobes</scene> and <scene name='70/706261/Cv/9'>contains the nucleotide and Mg+2 ion</scene><ref>PMID:18339619</ref>. An <scene name='70/706261/Cv/10'>Asp residue is the catalytic base residue</scene>. Water molecules are shown as red spheres. |
</StructureSection> | </StructureSection> | ||
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[[4pni]] – bRK + inhibitor <br /> | [[4pni]] – bRK + inhibitor <br /> | ||
[[4wbo]] – bRK + almexanox <br /> | [[4wbo]] – bRK + almexanox <br /> | ||
| - | [[2i94]] – bRK + recoverin - NMR [[ | + | [[2i94]] – bRK + recoverin - NMR <br /> |
| + | [[7mt9]] – bRK + rhodpsin + retinal – Cryo EM <br /> | ||
| + | [[7mtb]] – bRK (mutant) + rhodpsin + retinal – Cryo EM <br /> | ||
| + | [[7mta]] – bRK (mutant) + rhodpsin + retinal + antibody – Cryo EM <br /> | ||
| + | [[7mt8]] – bRK (mutant) + rhodpsin + retinal + antibiotic – Cryo EM <br /> | ||
== References == | == References == | ||
<references/> | <references/> | ||
| + | [[Category:Topic Page]] | ||
Current revision
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3D Structures of rhodopsin kinase
Updated on 17-October-2023
3c4y – bRK – bovine
3t8o – bRK (mutant)
3c4w, 3c4x – bRK + ATP
3c4z, 3c50, 3c51 – bRK + ADP
3qc9 – bRK (mutant) + ADP
4l9i – bRK + paroxetine
4pni – bRK + inhibitor
4wbo – bRK + almexanox
2i94 – bRK + recoverin - NMR
7mt9 – bRK + rhodpsin + retinal – Cryo EM
7mtb – bRK (mutant) + rhodpsin + retinal – Cryo EM
7mta – bRK (mutant) + rhodpsin + retinal + antibody – Cryo EM
7mt8 – bRK (mutant) + rhodpsin + retinal + antibiotic – Cryo EM
References
- ↑ Kuhn H, Wilden U. Deactivation of photoactivated rhodopsin by rhodopsin-kinase and arrestin. J Recept Res. 1987;7(1-4):283-98. PMID:3040978
- ↑ Yamamoto S, Sippel KC, Berson EL, Dryja TP. Defects in the rhodopsin kinase gene in the Oguchi form of stationary night blindness. Nat Genet. 1997 Feb;15(2):175-8. PMID:9020843 doi:http://dx.doi.org/10.1038/ng0297-175
- ↑ Singh P, Wang B, Maeda T, Palczewski K, Tesmer JJ. Structures of rhodopsin kinase in different ligand states reveal key elements involved in G protein-coupled receptor kinase activation. J Biol Chem. 2008 May 16;283(20):14053-62. Epub 2008 Mar 13. PMID:18339619 doi:10.1074/jbc.M708974200
