Pseudouridine synthase

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:20, 5 December 2019) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
<StructureSection load='1ze2' size='400' side='right' caption='tRNA pseudouridine synthase complex with RNA and pseudouridine monophosphate [[1ze2]]' scene='46/465439/Cv/1' pspeed='8'>
+
<StructureSection load='1ze2' size='400' side='right' caption='tRNA pseudouridine synthase complex with RNA that contains pseudouridine monophosphate [[1ze2]]' scene='46/465439/Cv/1' pspeed='8'>
-
== Function ==
+
__TOC__
-
'''tRNA pseudouridine synthase''' (PUS) catalyzes the conversion of tRNA uridine to tRNA pseudouridine<ref>PMID:14566049</ref>. PUS are active in the ribosomal large subunit (Rlu) and ribosomal small subunit (Rsu). '''Probable tRNA pseudouridine synthase''' (PPUS) is a similar enzyme.
+
==Function==
 +
[[Pseudouridine]] is an abundant modified nucleoside found in RNA molecules. It is formed post-transcriptionally in RNA molecules by isomerization of the bond between base and ribose in uridine. There are two major types of enzymes that catalyze this reaction:
-
== Structural highlights ==
+
* [[RNA-guided pseudouridine synthase]] -- Some ribonucleoproteins (complexes of proteins and small RNAs) have been identified in archaea and eukaryotes to catalyze these reactions. The small RNA in the complex serves as a guide element to select the site of modification through base-pairing with the target RNA molecule.
-
The conversion of uridine to pseudouridine causes conformational change of PUS<ref>PMID:14990747</ref>. <scene name='46/465439/Cv/3'>tRNA pseudouridine synthase binding with pseudouridine monophosphate</scene> ([[1ze2]]).
+
-
</StructureSection>
+
-
== 3D Structures of tRNA pseudouridine synthase==
+
-
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+
* [[Guide-independent Pseudouridine synthase|Guide-independent pseudouridine synthase]] -- The alternative to using the RNA-guided mechanism for pseudouridine formation is a [[Guide-independent Pseudouridine synthase|guide-independent mechanism]] acting through protein-only enzymes to catalyze the isomerization.
-
{{#tree:id=OrganizedByTopic|openlevels=0|
+
-
*tRNA pseudouridine synthase
+
== Structural highlights ==
 +
The conversion of uridine to pseudouridine is accompanied by a conformational change of pseudouridine synthase<ref>PMID:14990747</ref>. <scene name='46/465439/Cv/4'>tRNA pseudouridine synthase binding with pseudouridine monophosphate</scene> ([[1ze2]]).
 +
== 3D Structures of pseudouridine synthase ==
 +
[[Pseudouridine synthase 3D structures]]
-
**[[1dj0]] – EcPUS I – ''Escherichia coli''<BR />
+
</StructureSection>
-
**[[1ksk]], [[1ksl]] – EcPUS RsuA<BR />
+
-
**[[1vio]] - PUS RsuA – ''Haemophilus influenzae''<BR />
+
-
**[[1xpi]] - EcPUS RluC catalytic domain<BR />
+
-
**[[1v9k]] - EcPUS RluC C-terminal domain (mutant) <BR />
+
-
**[[2ist]] - EcPUS RluD <BR />
+
-
**[[1prz]], [[1qyu]], [[1v9f]] - EcPUS RluD catalytic domain<BR />
+
-
**[[2olw]] - EcPUS RluE<BR />
+
-
**[[2oml]] - EcPUS RluE catalytic domain<BR />
+
-
**[[2gml]] - EcPUS RluF catalytic domain<BR />
+
-
**[[4iqm]], [[4its]], [[4j37]], [[4nz6]] - hPUS Pus1 catalytic domain - human<br />
+
-
**[[4nz7]] - hPUS Pus1 catalytic domain (mutant)<br />
+
-
**[[1vs3]] – PUS TruA – ''Thermus thermophilus''<BR />
+
-
**[[1sgv]] – PUS TruB – ''Mycobacterium tuberculosis''<BR />
+
-
**[[1si7]], [[1szw]], [[1sb7]] - EcPUS TruD
+
-
 
+
-
*tRNA pseudouridine synthase complex with RNA
+
-
 
+
-
**[[1k8w]] – EcPUS B + stem-loop RNA<BR />
+
-
**[[1zl3]] - EcPUS B (mutant) + stem-loop RNA<BR />
+
-
**[[2i82]] – EcPUS RluA + RNA<BR />
+
-
**[[3dh3]] - EcPUS RluF + RNA<BR />
+
-
**[[2nqp]], [[2nr0]], [[2nre]] – EcPUS TruA + Leu-tRNA<BR />
+
-
**[[3hjw]], [[3lwo]], [[3lwp]], [[3lwq]], [[3lwr]], [[3lwv]] – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA – ''Pyrococcus furiosus''<BR />
+
-
**[[3mqk]] - PfPUS Cbf5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA<BR />
+
-
**[[3hjy]] - PfPUS Cbf5 + ribosome biogenesis protein NOP10 + RNA<BR />
+
-
**[[1ze2]] - TmPUS B + RNA<BR />
+
-
**[[2ab4]] - TmPUS B (mutant) + RNA<BR />
+
-
**[[1r3e]], [[1r3f]] - EcPUS B + RNA<BR />
+
-
*Probable tRNA pseudouridine synthase
 
-
**[[2apo]] – PPUS + ribosome biogenesis protein Nop10 – ''Methanocaldococcus jannaschii''<br />
 
-
**[[2ey4]] - PfPPUS Cbf5 + ribosome biogenesis protein Nop10 + small nucleolar RNP <br />
 
-
**[[2hvy]], [[3hax]] - PfPPUS B + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA<br />
 
-
**[[2rfk]] - PfPUS B + ribosome biogenesis protein Nop10 + small nucleolar RNP + RNA<br />
 
-
**[[3hay]] - PfPPUS B + ribosome biogenesis protein Nop10 + ribosomal protein L7 + small nucleolar RNP + RNA<br />
 
-
**[[2aus]] – PPUS B + ribosome biogenesis protein Nop10 – ''Pyrococcus abyssi''<br />
 
-
**[[1z2z]] – PPUS D – ''Methanosarcina mazei''<br />
 
-
}}
 
-
== References ==
 
-
<references/>
 
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

tRNA pseudouridine synthase complex with RNA that contains pseudouridine monophosphate 1ze2

Drag the structure with the mouse to rotate
Retrieved from "http://52.214.119.220/wiki/index.php/Pseudouridine_synthase"
Personal tools