Arabinanase

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== Function ==
== Function ==
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'''Arabinanase''' (Ara) hydrolyzes the α-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans.<ref>PMID:19505290</ref> See more details in [[Exo-alpha-1,5-L-Arabinanase]].
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'''Arabinanase''' (Ara) hydrolyzes the α-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans.<ref>PMID:19505290</ref> See more details in [[Exo-alpha-1,5-L-Arabinanase]]. '''Endo-arabinanase''' hydrolyzes from the N-terminal of the chain while '''exo-arabinanase''' hydrolyzes from the end of the chain.
== Structural highlights ==
== Structural highlights ==
The <scene name='45/451702/Cv/4'>active site of Ara is composed of 3 acidic residues</scene>.<ref>PMID:12198486</ref>. <scene name='45/451702/Cv/5'>Whole active site</scene>.
The <scene name='45/451702/Cv/4'>active site of Ara is composed of 3 acidic residues</scene>.<ref>PMID:12198486</ref>. <scene name='45/451702/Cv/5'>Whole active site</scene>.
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*<scene name='45/451702/Cv/7'>Arabinohexose binding pocket</scene>.
*<scene name='45/451702/Cv/6'>Cl coordination site</scene>.
*<scene name='45/451702/Cv/6'>Cl coordination site</scene>.
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</StructureSection>
 
== 3D Structures of Arabinanase ==
== 3D Structures of Arabinanase ==
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[[Arabinanase 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*Endo-arabinanase
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**[[2x8f]], [[1uv4]] – BsEndo-Ara – ''Bacillus subtilis''<br />
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**[[2x8t]], [[4cot]] - BsEndo-Ara (mutant) <br />
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**[[3cu9]], [[1wl7]], [[5ho0]], [[5ho2]], [[5hp6]] – GsEndo-Ara – ''Geobacillus stearothermophilus''<br />
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**[[3d5y]], [[3d60]] - GsEndo-Ara (mutant) <br />
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**[[1gyd]] – CcEndo-Ara – ''Cellvibrio cellulose''<br />
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**[[1gyh]] - CcEndo-Ara (mutant)<br />
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**[[4kc7]], [[4kc8]] - TpEndo-Ara – ''Thermotoga petrophila''<br />
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**[[4kca]], [[4kcb]] - Endo-Ara – bovine<br />
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*Endo-arabinanase complex with substrate
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**[[1gye]] - CcEndo-Ara (mutant) + arabinohexose<br />
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**[[3d5z]], [[3d61]] - GsEndo-Ara (mutant) + arabinotriose<br />
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**[[5ho9]] - GsEndo-Ara + arabinooctaose<br />
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**[[5hof]] - GsEndo-Ara + pentaose<br />
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**[[5hon]] - GsEndo-Ara + arabinotriose<br />
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**[[2x8s]] - BsEndo-Ara (mutant) + arabinotriose<br />
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*Exo-arabinanase
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**[[3a71]] – PcExo-Ara residues 24-378 – ''Penicillium chrysogenum''<br />
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**[[2w5n]] – GzExo-Ara – ''Gibberella zeae''
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*Exo-arabinanase complex with substrate
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**[[3a72]] - PcExo-Ara residues 24-378 + arabinobiose<br />
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**[[2w5o]] - GzExo-Ara + arabinobiose<br />
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}}
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== References ==
== References ==

Current revision

Endo-arabinanase complex with arabinohexose (yellow) and Cl- ion (green) (PDB code 1gye)

Drag the structure with the mouse to rotate

References

  1. ↑ Alhassid A, Ben-David A, Tabachnikov O, Libster D, Naveh E, Zolotnitsky G, Shoham Y, Shoham G. Crystal structure of an inverting GH 43 1,5-alpha-L-arabinanase from Geobacillus stearothermophilus complexed with its substrate. Biochem J. 2009 Jul 29;422(1):73-82. PMID:19505290 doi:10.1042/BJ20090180
  2. ↑ Nurizzo D, Turkenburg JP, Charnock SJ, Roberts SM, Dodson EJ, McKie VA, Taylor EJ, Gilbert HJ, Davies GJ. Cellvibrio japonicus alpha-L-arabinanase 43A has a novel five-blade beta-propeller fold. Nat Struct Biol. 2002 Sep;9(9):665-8. PMID:12198486 doi:10.1038/nsb835

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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