Collagenase (non-MMP)
From Proteopedia
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'''Collagenase''' (Col) catalyzes the breaking of peptide bonds in collagen. Col cleaves pro-collagen to create collagen. Some collagenases are part of the [[Matrix metalloproteinase]] family. | '''Collagenase''' (Col) catalyzes the breaking of peptide bonds in collagen. Col cleaves pro-collagen to create collagen. Some collagenases are part of the [[Matrix metalloproteinase]] family. | ||
| + | *'''Collagenase G''' recognises and unravels collagen microfibrils into triple helices and unwind them<ref>PMID:21947205</ref>. | ||
| + | *'''Collagenase H''' targets collagen III<ref>PMID:23768818</ref>. | ||
== Relevance == | == Relevance == | ||
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== Structural highlights == | == Structural highlights == | ||
| - | ''Clostridium histolycum'' collagenase contains several domains among them: peptidase domain (residues 331-721), polycystic kidney disease domain (PKD residues 792-880), collagen-binding domain (CBD residues 1003-1118). The peptidase domain contains a <scene name='47/477092/Cv/ | + | ''Clostridium histolycum'' collagenase contains several domains among them: peptidase domain (residues 331-721), polycystic kidney disease domain (PKD residues 792-880), collagen-binding domain (CBD residues 1003-1118). The peptidase domain contains a <scene name='47/477092/Cv/8'>Zn+2 coordination site</scene>, a <scene name='47/477092/Cv/9'>substrate-recognition site</scene> and an <scene name='47/477092/Cv/10'>oxyanion hole</scene>.<ref>PMID:23703618</ref> Water molecules are shown as red spheres. <scene name='47/477092/Cv/11'>Click here to see surface of binding site</scene>. |
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== 3D Structures of collagenase == | == 3D Structures of collagenase == | ||
| + | [[Collagenase 3D structures]] | ||
| - | + | </StructureSection> | |
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| - | **[[1azz]] – Col + ecotin – fiddler crab<br /> | ||
| - | **[[1hyl]], [[2hlc]] – Col – ''Hypoderma lineatum''<br /> | ||
| - | **[[5sv5]] – Col C-terminal – ''Bacillus anthracis''<br /> | ||
| - | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Current revision
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References
- ↑ Eckhard U, Schonauer E, Nuss D, Brandstetter H. Structure of collagenase G reveals a chew-and-digest mechanism of bacterial collagenolysis. Nat Struct Mol Biol. 2011 Sep 25;18(10):1109-14. doi: 10.1038/nsmb.2127. PMID:21947205 doi:10.1038/nsmb.2127
- ↑ Fujio A, Murayama K, Yamagata Y, Watanabe K, Imura T, Inagaki A, Ohbayashi N, Shima H, Sekiguchi S, Fujimori K, Igarashi K, Ohuchi N, Satomi S, Goto M. Collagenase H is crucial for isolation of rat pancreatic islets. Cell Transplant. 2014;23(10):1187-98. PMID:23768818 doi:10.3727/096368913X668654
- ↑ Eckhard U, Schonauer E, Brandstetter H. Structural basis for activity regulation and substrate preference of clostridial collagenases G, H, and T. J Biol Chem. 2013 May 23. PMID:23703618 doi:10.1074/jbc.M112.448548
