Shikimate dehydrogenase

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<StructureSection load='' size='350' side='right' scene='54/540162/Cv/1' caption='Shikimate dehydrogenase dimer complex with shikimate, sulfate and NADP [[2ev9]]'>
<StructureSection load='' size='350' side='right' scene='54/540162/Cv/1' caption='Shikimate dehydrogenase dimer complex with shikimate, sulfate and NADP [[2ev9]]'>
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__TOC__
== Function ==
== Function ==
'''Shikimate dehydrogenase''' (AroE) catalyzes the conversion of shikimate and NADP+ to 3-dihydroshikimate, NADPH and H+. It is part of the shikimate pathway which is responsible for the biosynthesis of phenylalanine, tyrosine and tryptophan. This pathway is found in bacteria, fungi, plants, algae and parasites and is missing in animals and humans<ref>PMID:18669580</ref>.
'''Shikimate dehydrogenase''' (AroE) catalyzes the conversion of shikimate and NADP+ to 3-dihydroshikimate, NADPH and H+. It is part of the shikimate pathway which is responsible for the biosynthesis of phenylalanine, tyrosine and tryptophan. This pathway is found in bacteria, fungi, plants, algae and parasites and is missing in animals and humans<ref>PMID:18669580</ref>.
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==Structural highlights ==
==Structural highlights ==
AroE changes its conformation from an open to closed one upon binding of the cofactor NADP. The active site contains the substrate shikimate<ref>PMID:17825835</ref>.
AroE changes its conformation from an open to closed one upon binding of the cofactor NADP. The active site contains the substrate shikimate<ref>PMID:17825835</ref>.
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*<scene name='54/540162/Cv/2'>NADP binding site</scene>. Water molecules shown as red spheres.
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*<scene name='54/540162/Cv/5'>NADP binding site</scene>. Water molecules are shown as red spheres.
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*<scene name='54/540162/Cv/4'>Whole binding site</scene>.
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*<scene name='54/540162/Cv/6'>Whole binding site</scene>.
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</StructureSection>
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== 3D Structures of shikimate dehydrogenase ==
== 3D Structures of shikimate dehydrogenase ==
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[[Shikimate dehydrogenase 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*Shikimate dehydrogenase
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**[[1p74]], [[1p77]] – AroE – ''Haemophilus influenzae''<br />
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**[[1wxd]] – TtAroE – ''Thermus thermophilus'' <br />
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**[[2hk7]], [[2hk8]] – AaAroE – ''Aquifex aeolicus'' <br />
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**[[2egg]] – AroE – ''Geobacillus kaustophilus'' <br />
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**[[3fbt]] – AroE/chorismate mutase – ''Clostridium acetobutylicum'' <br />
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**[[3don]] – SeAroE – ''Staphylococcus epidermidis'' <br />
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**[[3o8q]] – VcAroE – ''Vibrio cholerae'' <br />
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**[[3pwz]], [[4omu]] – AroE 3 – ''Pseudomonas putida'' <br />
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**[[3phg]] – HpAroE – ''Helicobacter pylori'' <br />
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**[[4foo]], [[4fpx]] – HpAroE (mutant) <br />
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**[[3u62]] – AroE – ''Thermotoga maritima'' <br />
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**[[2nlo]] – CgAroE – ''Corynebacterium glutamicum''<br />
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**[[4p4n]] - MtAroE – ''Mycobacterium tuberculosis''<br />
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**[[4xij]] - AroE – ''Mycobacterium fortuitum''<br />
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**[[5dzs]] - AroE – ''Peptoclostridium difficile''<br />
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*Shikimate dehydrogenase complex with NADP
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**[[1nyt]], [[1vi2]] – EcAroE + NADP – ''Escherichia coli''<br />
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**[[1nvt]] – MjAroE + NADP – ''Methanocaldococcus jannaschii''<br />
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**[[2cy0]] – TtAroE + NADP <br />
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**[[3toz]] – LmAroE + NAD – ''Listeria monocytogenes''<br />
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**[[3t4e]] – AroE + NAD – ''Salmonella enterica''<br />
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**[[3jyo]] - CgAroE + NAD <br />
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*Shikimate dehydrogenase complex with shikimate
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**[[2d5c]] – TtAroE + shikimate <br />
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**[[3doo]] – SeAroE + shikimate <br />
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**[[3pgj]] – VcAroE + shikimate <br />
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**[[3phh]] – HpAroE + shikimate <br />
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**[[4fos]], [[4fq8]], [[4fr5]], [[4fsh]] – HpAroE (mutant) + shikimate <br />
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**[[3phj]] – HpAroE + 3-dihydroshikimate <br />
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**[[4p4g]], [[4p4l]] - MtAroE + shikimate <br />
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*Shikimate dehydrogenase complex with NADP and shikimate
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**[[2ev9]] – TtAroE + shikimate + NADP <br />
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**[[2hk9]] – AaAroE + shikimate + NADP <br />
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**[[3sef]] – VcAroE + shikimate + NADP <br />
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**[[3tnl]] – LmAroE + shikimate + NAD <br />
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**[[3phi]] – HpAroE + shikimate + NADP <br />
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**[[3jyp]] - CgAroE + quinate + NAD <br />
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**[[3jyq]] - CgAroE + shikimate + NAD <br />
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}}
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== References ==
== References ==
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</StructureSection>
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Shikimate dehydrogenase dimer complex with shikimate, sulfate and NADP 2ev9

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  1. ↑ Singh S, Stavrinides J, Christendat D, Guttman DS. A phylogenomic analysis of the shikimate dehydrogenases reveals broadscale functional diversification and identifies one functionally distinct subclass. Mol Biol Evol. 2008 Oct;25(10):2221-32. doi: 10.1093/molbev/msn170. Epub 2008 Jul, 31. PMID:18669580 doi:http://dx.doi.org/10.1093/molbev/msn170
  2. ↑ Bagautdinov B, Kunishima N. Crystal structures of shikimate dehydrogenase AroE from Thermus thermophilus HB8 and its cofactor and substrate complexes: insights into the enzymatic mechanism. J Mol Biol. 2007 Oct 19;373(2):424-38. Epub 2007 Aug 21. PMID:17825835 doi:10.1016/j.jmb.2007.08.017

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