Acyl carrier protein
From Proteopedia
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<StructureSection load='' size='350' side='right' caption='Structure of E. coli acyl carrier protein complex with thioester, Zn+2 (grey) and Na+ (purple) ions (PDB code [[2fad]]).' scene='59/592131/Cv/1'> | <StructureSection load='' size='350' side='right' caption='Structure of E. coli acyl carrier protein complex with thioester, Zn+2 (grey) and Na+ (purple) ions (PDB code [[2fad]]).' scene='59/592131/Cv/1'> | ||
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== Function == | == Function == | ||
'''Acyl carrier protein''' (ACP) is a component of the fatty acid biosynthesis cycle. ACP catalyzes the addition of a thioester to a phosphopantetheine moiety. The phosphopantetheine moiety is added post-translationally to ACP serine residue by ACP synthetase (ACPS). The phosphopantetheine contains a free SH group which binds acyl groups in the fatty acid biosynthesis as thioesters. The acyl groups from acetyl-CoA and malonyl-CoA are transferred to ACP. There are 2 types of ACP. '''ACP I''' is a multifunctional polypeptide found in yeast and mammals while '''ACP II''' is a monomeric protein found in bacteria and plants.<ref>PMID:18059524</ref> | '''Acyl carrier protein''' (ACP) is a component of the fatty acid biosynthesis cycle. ACP catalyzes the addition of a thioester to a phosphopantetheine moiety. The phosphopantetheine moiety is added post-translationally to ACP serine residue by ACP synthetase (ACPS). The phosphopantetheine contains a free SH group which binds acyl groups in the fatty acid biosynthesis as thioesters. The acyl groups from acetyl-CoA and malonyl-CoA are transferred to ACP. There are 2 types of ACP. '''ACP I''' is a multifunctional polypeptide found in yeast and mammals while '''ACP II''' is a monomeric protein found in bacteria and plants.<ref>PMID:18059524</ref> | ||
| - | See also [[Molecular Playground/ACP apo]] | + | See also: |
| + | *[[Molecular Playground/ACP apo]] | ||
| + | *[[Acyl carrier protein synthase]] | ||
| + | *[[Lipid metabolism]] | ||
| + | *[[Fatty acid synthesis]] | ||
== Structural highlights == | == Structural highlights == | ||
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</StructureSection> | </StructureSection> | ||
| - | ==3D structures of acyl carrier protein== | ||
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| - | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| - | {{#tree:id=OrganizedByTopic|openlevels=0| | ||
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| - | *Acyl carrier protein | ||
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| - | **[[1l0h]], [[1t8k]] – EcACP – ''Escherichia coli'' <br /> | ||
| - | **[[1l0i]] – EcACP (mutant)<br /> | ||
| - | **[[1hy8]] – BsACP – ''Bacillus subtilis''<br /> | ||
| - | **[[1vku]] – ACP – ''Thermotoga maritima''<br /> | ||
| - | **[[1x3o]] – ACP – ''Thermus thermophilus''<br /> | ||
| - | **[[2fq0]], [[3gzl]], [[3gzm]] – PfACP – ''Plasmodium falciparum''<br /> | ||
| - | **[[2qnw]] – ACP residues 100-180 – ''Toxoplasma gondii''<br /> | ||
| - | **[[2ehs]], [[2eht]] – ACP – ''Aquifex aeolicus''<br /> | ||
| - | **[[3ce7]] – ACP – ''Toxoplasma'' <br /> | ||
| - | **[[5h9g]], [[5h9h]] – HpACP – ''Helicobacter pylori''<br /> | ||
| - | |||
| - | *Acyl carrier protein NMR structures | ||
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| - | **[[2kwl]] – ACP – ''Borrelia burgdorferi'' - NMR <br /> | ||
| - | **[[2l0q]] – ACP (mutant) – ''Vibrio harveyi'' - NMR<br /> | ||
| - | **[[2n57]] – ACP – ''Brucella melitensis'' - NMR <br /> | ||
| - | **[[2l4b]] – ACP – ''Anaplasma phagocytophilum'' - NMR <br /> | ||
| - | **[[2lol]] – ACP – ''Rickettsia prowazekii'' - NMR <br /> | ||
| - | **[[1acp]], [[2k92]], [[2k93]], [[2k94]] – EcACP - NMR<br /> | ||
| - | **[[1or5]] – ACP – ''Streptomyces roseofulvus'' - NMR<br /> | ||
| - | **[[2fq2]] – PfACP - NMR<br /> | ||
| - | **[[2cnr]] – ScACP – ''Streptomyces coelicolor'' - NMR<br /> | ||
| - | **[[2n98]] – ACP – ''Actinoplanes friulensis'' - NMR<br /> | ||
| - | **[[2n50]] – ACP – ''Enterococcus faecalis'' - NMR<br /> | ||
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| - | *Acyl carrier protein complex with fatty acid | ||
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| - | **[[2fac]], [[2fad]], [[2fae]] – EcACP + thioester <br /> | ||
| - | **[[2koo]], [[2kop]], [[2koq]], [[2kor]], [[2kos]] – ScACP + thioester - NMR<br /> | ||
| - | **[[2x2b]] – EcACP + malonate derivative <br /> | ||
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| - | *Acyl carrier protein complex with protein | ||
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| - | **[[2fhs]] – EcACP + enoyl-[ACP] reductase <br /> | ||
| - | **[[3ejb]], [[3ejd]], [[3eje]] – EcACP + thioester + p450BioI<br /> | ||
| - | **[[3ny7]] – EcACP + YchM STAS domain <br /> | ||
| - | **[[5vcb]] – EcACP + holo-[ACP] synthase <br /> | ||
| - | **[[5kof]] – EcACP + oxoacyl-[ACP] synthase <br /> | ||
| - | **[[5wgb]], [[5usr]] – EcACP + cysteine desulferase + LYR motif-containing protein 4 <br /> | ||
| - | **[[5wlw]], [[5wkp]] – EcACP + cysteine desulferase + LYR motif-containing protein 4 + ISCU<br /> | ||
| - | **[[1f80]] – BsACP + ACPS <br /> | ||
| - | **[[4dxe]] – ACP + ACPS – ''Staphylococcus aureus''<br /> | ||
| - | **[[4etw]] – ACP + pimelyl-[ACP] methyl ester esterase – ''Shigella flexneri''<br /> | ||
| - | **[[4ihf]], [[4ihg]], [[4ihh]] – EcACP + protein FIRA <br /> | ||
| - | **[[4keh]] – EcACP + fatty acid dehydratase <br /> | ||
| - | **[[4zjb]] – HpACP + FabZ<br /> | ||
| - | **[[5czd]] – ACP + malonyl-CoA-ACP transacylase – ''Streptomyces halstedii''<br /> | ||
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| - | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Current revision
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References
- ↑ Byers DM, Gong H. Acyl carrier protein: structure-function relationships in a conserved multifunctional protein family. Biochem Cell Biol. 2007 Dec;85(6):649-62. PMID:18059524 doi:http://dx.doi.org/10.1139/o07-109
- ↑ Roujeinikova A, Simon WJ, Gilroy J, Rice DW, Rafferty JB, Slabas AR. Structural studies of fatty acyl-(acyl carrier protein) thioesters reveal a hydrophobic binding cavity that can expand to fit longer substrates. J Mol Biol. 2007 Jan 5;365(1):135-45. Epub 2006 Sep 23. PMID:17059829 doi:10.1016/j.jmb.2006.09.049
