6izj
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structural characterization of mutated NreA protein in nitrate binding site from Staphylococcus aureus== | |
| + | <StructureSection load='6izj' size='340' side='right'caption='[[6izj]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6izj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IZJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IZJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6izj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6izj OCA], [https://pdbe.org/6izj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6izj RCSB], [https://www.ebi.ac.uk/pdbsum/6izj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6izj ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0H3JU14_STAAM A0A0H3JU14_STAAM] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Staphylococcus aureus is an anaerobic facultative microorganism that features the NreABC system for nitrate respiration. NreB is the sensor histidine kinase that phosphorylates the response regulator NreC to stimulate the expression of target genes. NreA is a nitrate sensor which dissociates from NreB in the present of nitrate and relieves its inhibition on NreB. However, the molecular basis of how NreA regulate NreB remains unknown. In this study, we determined the crystal structures of nitrate-bound NreA from S. aureus (SaNreA/NO3(-)) and its apoNreA-like mutant SaNreAY(94A) in complex with ethanediol (SaNreA(Y94A)/EDO). Structural comparison reveals that the C-terminal loop in SaNreA/NO3(-) rearranges to an alpha-helix (alpha7) in SaNreA(Y94A)/EDO, which converts an acidic pocket on the surface to a positively charged region. This conformational change of SaNreA C-terminus might play a role in SaNreB binding. | ||
| - | + | Structural insights into the conformational change of Staphylococcus aureus NreA at C-terminus.,Sangare L, Chen W, Wang C, Chen X, Wu M, Zhang X, Zang J Biotechnol Lett. 2020 Jan 22. pii: 10.1007/s10529-020-02807-2. doi:, 10.1007/s10529-020-02807-2. PMID:31970556<ref>PMID:31970556</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6izj" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Sangare | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Staphylococcus aureus subsp. aureus Mu50]] |
| + | [[Category: Chen W]] | ||
| + | [[Category: Chen X]] | ||
| + | [[Category: Sangare L]] | ||
| + | [[Category: Wang C]] | ||
| + | [[Category: Wu M]] | ||
| + | [[Category: Zang J]] | ||
| + | [[Category: Zhang X]] | ||
Current revision
Structural characterization of mutated NreA protein in nitrate binding site from Staphylococcus aureus
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