7me1
From Proteopedia
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==YfeA oligomer crystal 1, form 1== | ==YfeA oligomer crystal 1, form 1== | ||
| - | <StructureSection load='7me1' size='340' side='right'caption='[[7me1]]' scene=''> | + | <StructureSection load='7me1' size='340' side='right'caption='[[7me1]], [[Resolution|resolution]] 2.05Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ME1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ME1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7me1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ME1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ME1 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7me1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7me1 OCA], [https://pdbe.org/7me1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7me1 RCSB], [https://www.ebi.ac.uk/pdbsum/7me1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7me1 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7me1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7me1 OCA], [https://pdbe.org/7me1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7me1 RCSB], [https://www.ebi.ac.uk/pdbsum/7me1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7me1 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/YFEA_YERPE YFEA_YERPE] Part of an ATP-driven transport system YfeABCD for chelated iron. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The substrate-binding protein YfeA (also known as YPO2439 or y1897) is a polyspecific metal-binding protein that is crucial for nutrient acquisition and virulence in Yersinia pestis, the causative microbe of plague. YfeA folds into a monomeric c-clamp like other substrate-binding proteins and has two metal-binding sites (sites 1 and 2). Site 2 is a bidentate surface site capable of binding Zn and Mn atoms and is a unique feature of YfeA. Occasionally, the site 2 residues of two YfeA molecules will cooperate with the histidine tag of a third YfeA molecule in coordinating the same metal and lead to metal-dependent crystallographic packing. Here, three crystal structures of YfeA are presented at 1.85, 2.05 and 2.25 A resolution. A comparison of the structures reveals that the metal can be displaced at five different locations ranging from approximately 4 to approximately 16 A away from the canonical site 2. These observations reveal different configurations of site 2 that enable cooperative metal binding and demonstrate how site 2 is dynamic and freely available for inter-protein metal coordination. | ||
| + | |||
| + | Site 2 of the Yersinia pestis substrate-binding protein YfeA is a dynamic surface metal-binding site.,Radka CD, Aller SG Acta Crystallogr F Struct Biol Commun. 2021 Sep 1;77(Pt 9):286-293. doi:, 10.1107/S2053230X21008086. Epub 2021 Aug 24. PMID:34473105<ref>PMID:34473105</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7me1" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Yersinia pestis]] | ||
[[Category: Aller SG]] | [[Category: Aller SG]] | ||
[[Category: Radka CD]] | [[Category: Radka CD]] | ||
Current revision
YfeA oligomer crystal 1, form 1
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