Journal:Acta Cryst D:S2059798321009633

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:10, 20 June 2022) (edit) (undo)
 
(19 intermediate revisions not shown.)
Line 11: Line 11:
In this paper, we present the first structure of SorC DBDs bound to DNA duplexes. We show DBD structures of representatives of each subfamily, <scene name='89/895271/Cv/4'>DeoR</scene> and <scene name='89/895271/Cv/7'>CggR</scene>, and compare their binding mode, which is likely common to all SorC family members.
In this paper, we present the first structure of SorC DBDs bound to DNA duplexes. We show DBD structures of representatives of each subfamily, <scene name='89/895271/Cv/4'>DeoR</scene> and <scene name='89/895271/Cv/7'>CggR</scene>, and compare their binding mode, which is likely common to all SorC family members.
-
*<scene name='89/895271/Cv/7'>A cartoon representation of the bsCggRDBD dimer bound to the DNA operator</scene>. bsCggRDBD comprises five α-helices and two β-strands, with a topology of four conse�cutive �-helices �1 (1–13), �2 (15–32), �3 (37–43) and �4 (48–
+
*<scene name='89/895271/Cv/4'>A cartoon representation of the bsDeoRDBD dimer bound to the DNA operator</scene>. First monomer is colored in red and the second one is in salmon.
-
61) followed by a �-hairpin (called a wing) formed by β1 (64–
+
*<scene name='89/895271/Cv/7'>A cartoon representation of the bsCggRDBD dimer bound to the DNA operator</scene>. ''bs''CggRDBD comprises five α-helices and two β-strands, with a topology of four α-helices α1 (1–13), α2 (15–32), α3 (37–43) and α4 (48–61) followed by <scene name='89/895271/Cv/8'>a β-hairpin (called a wing) formed by β1 (64–66) and β2 (71–73)</scene> and continuing with a final α-helix (75–89) that links the N-terminal DBD to the C-terminal EBD (α-helices are colored in red, β-strands are in yellow and second monomer is in salmon).
-
66) and β2 (71–73) and continuing with a final �-helix (75–89)
+
 
-
that links the N-terminal DBD to the C-terminal EBD.
+
*<scene name='89/895271/Cv/15'>Morph showing the conformational change of bsDeoR-DBD upon DNA binding</scene>. DNA-free ''bs''DeoRDBD is colored in pink and DNA-bound ''bs''DeoRDBD is in red. <jmol><jmolButton>
 +
<script>if (_animating); anim pause;set echo bottom left; color echo white; font echo 20 sansserif;echo Animation Paused; else; anim resume; set echo off;endif;</script>
 +
<text>Stop Animation</text>
 +
</jmolButton></jmol>
 +
 
 +
'''PDB references:''' DNA-binding domain of DeoR in complex with DNA operator, [[7bhy]]; DNA-binding domain of CggR in complex with DNA operator, [[7oyk]].
<b>References</b><br>
<b>References</b><br>

Current revision

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

Alexander Berchansky, Jaime Prilusky

This page complements a publication in scientific journals and is one of the Proteopedia's Interactive 3D Complement pages. For aditional details please see I3DC.
Retrieved from "http://52.214.119.220/wiki/index.php/Journal:Acta_Cryst_D:S2059798321009633"
Personal tools