8jyz

From Proteopedia

(Difference between revisions)

Current revision

Cryo-EM structure of RCD-1 pore from Neurospora crassa

Structural highlights

8jyz is a 22 chain structure with sequence from Neurospora crassa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.63Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RCD11_NEUCR Gasdermin-like protein involved in heterokaryon incompatibility, a process that ensures that during spontaneous vegetative cell fusion, only compatible cells from the same colony survive (non-self-recognition) (PubMed:31636083, PubMed:32703806). In N.crassa, the rcd-1 locus exists as 2 incompatible alleles, rcd-1-1 (this entry) and rcd-1-2 (AC P0DW10) (PubMed:31636083). During the allorecognition process, forms a heterooligomer with rcd-1-2, thereby forming a functional gasdermin-like complex that binds to membranes and forms pores, triggering cell death (PubMed:32703806). Binds negatively charged phospholipids, such as cardiolipin and phosphatidylserine (PubMed:32703806). Also binds to phosphoinositides, preferentially to phosphatidylinositol-3-phosphate (PtdIns-3-P), PtdIns-5-P and PtdIns-3,5-P2 (PubMed:32703806).^[1] ^[2]

Publication Abstract from PubMed

Gasdermins (GSDMs) are pore-forming proteins that execute pyroptosis for immune defense. GSDMs are two-domain proteins, activated by proteolytic removal of the inhibitory domain. Here we report two types of cleavage-independent GSDM activation. First, TrichoGSDM, a pore-forming-domain-only protein from the basal metazoan Trichoplax adhaerens, is a disulfides-linked autoinhibited dimer, activated by reduction of the disulfides. Cryo-electron microscopy (cryo-EM) structure illustrates assembly mechanism for the 44-mer TrichoGSDM pore. Second, RCD-1-1/RCD-1-2, encoded by polymorphic rcd-1 in filamentous fungus Neurospora crassa, are also pore-forming-domain-only GSDMs. RCD-1-1 and RCD-1-2, when encountering each other, form pores and cause pyroptosis, underlying allorecognition in Neurospora. Cryo-EM structure reveals a pore of 11 RCD-1-1/RCD-1-2 heterodimers and heterodimerization-triggered pore assembly mechanism. This study shows mechanistic diversities in GSDM activation and indicates versatile functions of GSDMs.

Cleavage-independent activation of ancient eukaryotic gasdermins and structural mechanisms.,Li Y, Hou Y, Sun Q, Zeng H, Meng F, Tian X, He Q, Shao F, Ding J Science. 2024 Apr 25:eadm9190. doi: 10.1126/science.adm9190. PMID:38662913^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Daskalov A, Gladieux P, Heller J, Glass NL. Programmed Cell Death in Neurospora crassa Is Controlled by the Allorecognition Determinant rcd-1. Genetics. 2019 Dec;213(4):1387-1400. PMID:31636083 doi:10.1534/genetics.119.302617
↑ Daskalov A, Mitchell PS, Sandstrom A, Vance RE, Glass NL. Molecular characterization of a fungal gasdermin-like protein. Proc Natl Acad Sci U S A. 2020 Aug 4;117(31):18600-18607. PMID:32703806 doi:10.1073/pnas.2004876117
↑ Li Y, Hou Y, Sun Q, Zeng H, Meng F, Tian X, He Q, Shao F, Ding J. Cleavage-independent activation of ancient eukaryotic gasdermins and structural mechanisms. Science. 2024 Apr 25:eadm9190. PMID:38662913 doi:10.1126/science.adm9190

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/8jyz"

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 8jyz is ON HOLD
+==Cryo-EM structure of RCD-1 pore from Neurospora crassa==
+<StructureSection load='8jyz' size='340' side='right'caption='[[8jyz]], [[Resolution|resolution]] 3.63&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8jyz]] is a 22 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8JYZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8JYZ FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.63&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8jyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8jyz OCA], [https://pdbe.org/8jyz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8jyz RCSB], [https://www.ebi.ac.uk/pdbsum/8jyz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8jyz ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RCD11_NEUCR RCD11_NEUCR] Gasdermin-like protein involved in heterokaryon incompatibility, a process that ensures that during spontaneous vegetative cell fusion, only compatible cells from the same colony survive (non-self-recognition) (PubMed:31636083, PubMed:32703806). In N.crassa, the rcd-1 locus exists as 2 incompatible alleles, rcd-1-1 (this entry) and rcd-1-2 (AC P0DW10) (PubMed:31636083). During the allorecognition process, forms a heterooligomer with rcd-1-2, thereby forming a functional gasdermin-like complex that binds to membranes and forms pores, triggering cell death (PubMed:32703806). Binds negatively charged phospholipids, such as cardiolipin and phosphatidylserine (PubMed:32703806). Also binds to phosphoinositides, preferentially to phosphatidylinositol-3-phosphate (PtdIns-3-P), PtdIns-5-P and PtdIns-3,5-P2 (PubMed:32703806).<ref>PMID:31636083</ref> <ref>PMID:32703806</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Gasdermins (GSDMs) are pore-forming proteins that execute pyroptosis for immune defense. GSDMs are two-domain proteins, activated by proteolytic removal of the inhibitory domain. Here we report two types of cleavage-independent GSDM activation. First, TrichoGSDM, a pore-forming-domain-only protein from the basal metazoan Trichoplax adhaerens, is a disulfides-linked autoinhibited dimer, activated by reduction of the disulfides. Cryo-electron microscopy (cryo-EM) structure illustrates assembly mechanism for the 44-mer TrichoGSDM pore. Second, RCD-1-1/RCD-1-2, encoded by polymorphic rcd-1 in filamentous fungus Neurospora crassa, are also pore-forming-domain-only GSDMs. RCD-1-1 and RCD-1-2, when encountering each other, form pores and cause pyroptosis, underlying allorecognition in Neurospora. Cryo-EM structure reveals a pore of 11 RCD-1-1/RCD-1-2 heterodimers and heterodimerization-triggered pore assembly mechanism. This study shows mechanistic diversities in GSDM activation and indicates versatile functions of GSDMs.
-Authors:
+Cleavage-independent activation of ancient eukaryotic gasdermins and structural mechanisms.,Li Y, Hou Y, Sun Q, Zeng H, Meng F, Tian X, He Q, Shao F, Ding J Science. 2024 Apr 25:eadm9190. doi: 10.1126/science.adm9190. PMID:38662913<ref>PMID:38662913</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 8jyz" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Neurospora crassa]]
+[[Category: Ding J]]
+[[Category: Hou YJ]]
+[[Category: Li Y]]
+[[Category: Sun Q]]

8jyz

From Proteopedia

Current revision

Cryo-EM structure of RCD-1 pore from Neurospora crassa

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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